標題: Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring [打印本頁] 作者: Assert 時間: 2025-3-21 17:19
書目名稱Calcium Signalling影響因子(影響力)
書目名稱Calcium Signalling影響因子(影響力)學科排名
書目名稱Calcium Signalling網(wǎng)絡公開度
書目名稱Calcium Signalling網(wǎng)絡公開度學科排名
書目名稱Calcium Signalling被引頻次
書目名稱Calcium Signalling被引頻次學科排名
書目名稱Calcium Signalling年度引用
書目名稱Calcium Signalling年度引用學科排名
書目名稱Calcium Signalling讀者反饋
書目名稱Calcium Signalling讀者反饋學科排名
作者: Adj異類的 時間: 2025-3-21 22:57 作者: hereditary 時間: 2025-3-22 01:00
Patch-Clamp Analysis of the Mitochondrial Calcium Uniporter,ortant considerations in applying patch-clamp technique to such a small subcellular organelle. With small variations in the bath and pipette solution composition, the same methodology can be applied to study any other currents (e.g., H. or Cl.) from the mitochondrial inner membrane.作者: 注視 時間: 2025-3-22 07:52 作者: 減去 時間: 2025-3-22 11:54 作者: 緯線 時間: 2025-3-22 12:53 作者: 緯線 時間: 2025-3-22 17:22 作者: 不容置疑 時間: 2025-3-23 00:00
Untersuchungen zur Sichtfreihaltung,ortant considerations in applying patch-clamp technique to such a small subcellular organelle. With small variations in the bath and pipette solution composition, the same methodology can be applied to study any other currents (e.g., H. or Cl.) from the mitochondrial inner membrane.作者: 使服水土 時間: 2025-3-23 01:38
Fluchtmigration und berufliche Ausbildungs in the different subcellular compartments. Here we report a protocol that combines these techniques to study astrocyte Ca. signaling in response to somatostatin (SST)-expressing interneurons, one of the main classes of GABAergic inhibitory interneurons.作者: 無關緊要 時間: 2025-3-23 05:59 作者: Esophagitis 時間: 2025-3-23 13:44
,Der kernbasierte AD-Sch?tzer KADE,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).作者: 替代品 時間: 2025-3-23 16:42
,LEISTUNGSERSTELLUNG IN S?GEBETRIEBEN,l, using the electrophysiological technique of planar lipid bilayer..We showed that MCU gives rise to single-channel Ca. currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.作者: 弓箭 時間: 2025-3-23 21:10 作者: CUMB 時間: 2025-3-23 23:31
Peter Weise,Wolfgang Brandes,Manfred Kraftred contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca. handling in cardiomyocytes represents a central method in experimental molecular cardiology.作者: Little 時間: 2025-3-24 02:51 作者: 品嘗你的人 時間: 2025-3-24 08:32
Fluchtmigration und berufliche Ausbildung . parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca. assays.作者: 反抗者 時間: 2025-3-24 14:10
Neue Migrationen als Herausforderung? One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca. signaling from SOCE in human embryonic kidney (HEK) cells.作者: Wernickes-area 時間: 2025-3-24 18:33
,Webern, Sch?nberg und Strawinsky,ical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.作者: ADJ 時間: 2025-3-24 22:45
,Dieter Schnebel — Heinz Holliger, estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.作者: 貪婪性 時間: 2025-3-25 01:07
Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).作者: 牛馬之尿 時間: 2025-3-25 05:20
MCU Regulation in Lipid Bilayer and Electrophysiological Recording,l, using the electrophysiological technique of planar lipid bilayer..We showed that MCU gives rise to single-channel Ca. currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.作者: 學術討論會 時間: 2025-3-25 07:40
Electrophysiological Characterization of Calcium-Permeable Channels Using Planar Lipid Bilayer,protein complex responsible for the uptake of this ion into mitochondria..Here we describe the protocol used for the electrophysiological characterization of the mitochondrial calcium uniporter (MCU) complex: the following outline indicates step-by-step the setup of planar lipid bilayer experiments.作者: investigate 時間: 2025-3-25 14:35
Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes,red contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca. handling in cardiomyocytes represents a central method in experimental molecular cardiology.作者: 啪心兒跳動 時間: 2025-3-25 16:41 作者: SOBER 時間: 2025-3-25 22:14
Employing Transgenic Parasite Strains to Study the Ca, Dynamics in the Human Malaria Parasite ,, . parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca. assays.作者: fertilizer 時間: 2025-3-26 03:25 作者: 投票 時間: 2025-3-26 07:18
Assessing Calcium-Stimulated Mitochondrial Bioenergetics Using the Seahorse XF96 Analyzer,ical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.作者: RALES 時間: 2025-3-26 09:17 作者: 盤旋 時間: 2025-3-26 16:02 作者: 性滿足 時間: 2025-3-26 20:49
Textilforschungsanstalt Krefeldle alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in . root hairs.作者: 政府 時間: 2025-3-27 00:59 作者: cochlea 時間: 2025-3-27 01:53
Methods to Measure Intracellular Ca2+ Concentration Using Ca2+-Sensitive Dyes,will be first presented. Then, the implementation of Fura-2 to detect [Ca.]. in two specific cell types, namely, human adrenocortical cells and primary skin fibroblasts, will be discussed in more particulars. Finally, the procedure to monitor Ca. influx through the plasma membrane using Fura-2 will be described.作者: Watemelon 時間: 2025-3-27 07:52
In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in ,,le alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in . root hairs.作者: Haphazard 時間: 2025-3-27 12:40
Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes,the most prominent ROS (hydrogen peroxide, H.O.) using genetically encoded protein sensors and fluorescent dyes. We also provide guidelines on how to simultaneously detect Ca. and H.O. and how to examine the influence of Ca. signals on cellular ROS production and vice versa.作者: 確認 時間: 2025-3-27 16:45 作者: Indecisive 時間: 2025-3-27 20:22
Book 2019yses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introduction作者: Acumen 時間: 2025-3-27 23:08
Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibers by mitochondrial Ca. transients in the same fiber. Probe encoding plasmids are expressed in flexor digitorum brevis (FDB) muscles by means of the in vivo electroporation technique. Measurements are then performed ex vivo in isolated single myofibers.作者: 出來 時間: 2025-3-28 02:45
Book 2019bleshooting and avoiding known pitfalls..Comprehensive and cutting-edge, .Calcium Signalling: Methods and Protocols. is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research..作者: OTTER 時間: 2025-3-28 07:29 作者: 鴕鳥 時間: 2025-3-28 10:34
In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle,roscopy as well as force transducers and associated hardware for data acquisition. Information on how to determine subcellular localization of the genetically encoded Ca. sensors and on how to calibrate the ratiometric data in a semiquantitative manner is given in the final paragraphs.作者: Intuitive 時間: 2025-3-28 14:44 作者: paleolithic 時間: 2025-3-28 22:24 作者: Middle-Ear 時間: 2025-3-28 23:13 作者: 疼死我了 時間: 2025-3-29 05:35 作者: 先兆 時間: 2025-3-29 10:51 作者: visual-cortex 時間: 2025-3-29 12:13 作者: Obliterate 時間: 2025-3-29 19:08 作者: thrombus 時間: 2025-3-29 20:22
In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in ,,ng and phototoxicity as possible to the sample. Light sheet fluorescence microscopy offers these capabilities, with the further chance to mount the sample in vertical position, mimicking the plant’s growth and physiological conditions..A protocol for plant preparation and mounting in a light sheet m作者: 音樂會 時間: 2025-3-30 02:32
Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibers by urements are based on the use of genetically encoded probes. Addition of targeting DNA sequences, in frame with the probe encoding sequence, ensures protein expression in specific compartments. The use of probes with different excitation spectra allows the simultaneous determination of cytosolic and作者: 反應 時間: 2025-3-30 04:12 作者: Benign 時間: 2025-3-30 10:35
In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle,n. However, microscopic observation of Ca. signalling in live skeletal muscle tissue has been hampered, in particular, by the combination of the high speed of Ca. transients and the contractile properties that are inherent to muscle. The present chapter describes methods to visualize Ca. signals dur作者: 巧辦法 時間: 2025-3-30 14:32
TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis,al exocytosis and clearance of lysosomal accumulation in various cellular models of lysosomal storage disorders (LSDs). Here, we described methods to determine TFEB activation and lysosomal exocytosis that may represent innovative tools to study lysosomal function and to develop novel therapeutic ap作者: nocturia 時間: 2025-3-30 20:00 作者: Scintigraphy 時間: 2025-3-31 00:13 作者: Cabinet 時間: 2025-3-31 02:58
Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2,ions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-作者: DAUNT 時間: 2025-3-31 07:59
Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes,st abundant glial cells in the brain. Astrocytes respond to neurotransmitters with Ca. elevations which represent a key event in the modulation of local brain circuits played by these glial cells. Due to technical limitations, the study of Ca. signal dynamics in astrocytes has focused for decades al作者: 男學院 時間: 2025-3-31 09:47
Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes,. Reactive oxygen species (ROS) are important factors that influence these redox processes. Calcium ion (Ca.) dynamics and signals are also essential regulators of key cellular processes. Therefore, the combined and precise monitoring of ROS and Ca. in single cells, with a high spatial and temporal 作者: landfill 時間: 2025-3-31 15:02 作者: REIGN 時間: 2025-3-31 19:16
Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography ergetic effect of Ca. signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we d作者: Feigned 時間: 2025-4-1 00:31
Anna Raffaello,Denis Vecellio ReaneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 搖曳的微光 時間: 2025-4-1 03:06
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220789.jpg作者: engagement 時間: 2025-4-1 07:26
https://doi.org/10.1007/978-1-4939-9018-4Ca2+; Organelle-Targeted Aequorin-Based Probes; ; Sensitive Dyes; ; FRET; Toxoplasma作者: 小歌劇 時間: 2025-4-1 11:39
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: Felicitous 時間: 2025-4-1 17:00
Aerodynamische Entwicklungswerkzeuge,elles in intact cells. After the binding of Ca. to three high-affinity binding sites, an irreversible reaction occurs leading to the emission of photons that is proportional to [Ca.]. While native aequorin is suitable for measuring cytosolic [Ca.] after cell stimulation in a range from 0.5 to 10?μM,
