作者: pineal-gland 時間: 2025-3-22 00:02 作者: 愛得痛了 時間: 2025-3-22 03:39
Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.), however, these preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to cells. The author will describe a .Ca.-release assay u作者: muffler 時間: 2025-3-22 04:38
Microinjection of ,-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adhanalogs, such as photolabile “caged Ins(1,4,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.); however, microsomal preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to permea作者: 薄膜 時間: 2025-3-22 10:19 作者: 沉默 時間: 2025-3-22 14:10
1064-3745 ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for978-1-61737-573-6978-1-59259-949-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 沉默 時間: 2025-3-22 18:31 作者: 大暴雨 時間: 2025-3-22 21:50 作者: alliance 時間: 2025-3-23 02:32 作者: Enteropathic 時間: 2025-3-23 09:01
,Haltestellenplanung in St?dten,analogs, such as photolabile “caged Ins(1,4,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.); however, microsomal preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to permea作者: 合唱隊 時間: 2025-3-23 12:31
Modellbildung mit Exponentialfunktionen,his organelle with Ca., as reported by ER-trapped dye, could be directly visualized in single permeabilized cells with high spatiotemporal resolution (.,.). This basic approach has been adopted by a number of laboratories to investigate phenomena ranging from Ca. oscillations (.–.) to quantal releas作者: 得意牛 時間: 2025-3-23 15:17
Book 2005Latest edition. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for作者: GROSS 時間: 2025-3-23 20:46
Book 2005Latest editionpers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the vo作者: violate 時間: 2025-3-24 01:42
Neue Makro?konomik und Au?enhandelof protein kinase C isozymes, and Ins(1,4,5)P. mobilizes intracellular Ca.. G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLC-γ isozymes (.,.). Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P..作者: LAY 時間: 2025-3-24 02:20 作者: 脫毛 時間: 2025-3-24 09:25
https://doi.org/10.1007/978-3-642-69146-1ntagonists at a target of interest, making the system a valuable screening tool for interrogation of compound libraries. Typically, FLIPR can be used to screen more than 150 compound plates per day in a high-throughput screening environment equating to more than 50,000 compounds at a single concentration in a 384-well system.作者: right-atrium 時間: 2025-3-24 12:21
Ratiometric Ca2+ Measurements Using the FlexStation? Scanning Fluorometerr, under certain circumstances researchers may prefer to conduct high-throughput experiments with ratiometric dyes, particularly when issues of dye leakage, photobleaching, or signal-to-noise ratio become a concern.作者: 流浪 時間: 2025-3-24 18:11 作者: 通便 時間: 2025-3-24 22:04 作者: 路標(biāo) 時間: 2025-3-25 01:09
,Eigene Interpretation von § 84 Abs. 2 AMG,hese patterns cannot be observed in population studies since cells oscillate out of phase and at different frequencies, resulting in a smooth population average. It is only when cells are electrically well coupled that synchronized, population oscillations can be recorded, but this case is more the exception than the rule.作者: Figate 時間: 2025-3-25 06:44
Measurement of [Ca2+]i in Cell Suspensions Using Indo-1ally dyes that respond to Ca. with shifts of excitation or emission spectra (.–.). By measuring at two selected wavelengths (either dual emission or dual excitation), it is possible to calculate the proportion of dye in the Ca.-bound and Ca.-free forms.作者: indigenous 時間: 2025-3-25 11:03 作者: 起波瀾 時間: 2025-3-25 12:24
1064-3745 r Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! T作者: Delude 時間: 2025-3-25 16:56
Ulfert Herlyn,Gitta Scheller,Wulf Tessinrange of detection equipment that can be used. Consequently, this chapter is designed to provide the user with an overview of the technology in order that he or she can move toward developing a protocol that will suit the experimental objectives, the cells, and the equipment available to the investigator.作者: Precursor 時間: 2025-3-25 21:48 作者: Hippocampus 時間: 2025-3-26 02:04
Studies in Contemporary Economicsnd messenger. Through this relationship, activation of VSCCs is tightly coupled to the gamut of cellular functions dependent on intracellular Ca., including muscle contraction, energy metabolism, gene expression, and exocytotic/endocytotic cycling.作者: Enliven 時間: 2025-3-26 04:33 作者: achlorhydria 時間: 2025-3-26 10:52
Measurement of [3H]PN200-110 and [125I]ω-Conotoxin MVIIA Binding and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransmitter release (.). In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca2. channels to reduce Ca. influx (.,.).作者: 鑒賞家 時間: 2025-3-26 15:09 作者: Allure 時間: 2025-3-26 18:58 作者: 救護(hù)車 時間: 2025-3-26 23:06 作者: HAVOC 時間: 2025-3-27 03:39
Measurement of [Ca2+]i in Cell Suspensions Using Indo-1mulation of receptors, belonging to almost all the classes so far described, will result in changes in [Ca.].. These changes might be directly induced by either Ca.-influx or Ca.-mobilization from intracellular stores, or indirectly by a number of other mechanisms (.–.). The development of fluoresce作者: Strength 時間: 2025-3-27 05:52
Confocal Microscopyhow recent advances in this technology, allied with the continued development of Ca.-sensitive fluorescent probes, have provided us with methodologies for unravelling the complexities of Ca. signaling at the cellular and subcellular level. Specifically, we will provide a detailed methodology for the作者: 內(nèi)行 時間: 2025-3-27 09:26
Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentrationr cell parameters, but the usefulness of this approach is limited by several problems, not the least of which is temporal averaging. In population studies, agonists often evoke a characteristic peak and plateau type of response irrespective of the stimulus intensity. However, equivalent experiments 作者: Autobiography 時間: 2025-3-27 14:01
Ratiometric Ca2+ Measurements Using the FlexStation? Scanning Fluorometer measurements of intracellular Ca. concentration (.Chapter 7), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications, the primary limitation of FLIPR (i.e., its requirement for single wavelength fluorescent probes using vis作者: labyrinth 時間: 2025-3-27 20:23 作者: 輕快走過 時間: 2025-3-28 00:57 作者: Instantaneous 時間: 2025-3-28 03:41 作者: antipsychotic 時間: 2025-3-28 09:42
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocog inositol phospholipid-specific phospholipase C (PLC). Activated PLC catalyzes the hydrolysis of phosphatidylinositol bisphosphate (PIP.), a minor membrane phospholipid, to form two second messengers, diacylglycerol (DAG) and inositol (1,4,5)trisphosphate [Ins(1,4,5,)P.]. DAG is a direct activator 作者: Priapism 時間: 2025-3-28 12:53
Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assayiverse cellular processes including neuronal signaling, smooth muscle contraction, fertilization, and sensory perception (.). Ins(1,4,5)P. formation is triggered by the activation of a wide variety of seven-transmembrane, G protein-linked receptors, e.g., muscarinic, glutamate, dopamine, and opioid 作者: 沙發(fā) 時間: 2025-3-28 18:14
Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assayroduce the second messengers, inositol(1,4,5)trisphosphate [Ins(1,4,5)P.] and diacylglycerol (.). Ins(1,4,5)P. interacts with specific receptor populations of ligand-gated channels to mobilize nonmitochondrial intracellular calcium (Ca.) stores (.). Because Ins(1,4,5)P. is very hydrophilic, it canno作者: 苦笑 時間: 2025-3-28 19:17
Microinjection of ,-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adhosphoinositidase Cs (.). These enzymes catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to produce the second messengers, .-inositol(1,4,5)trisphosphate [Ins(1,4,5)P.] and diacylglycerol (.). Ins(1,4,5)P. interacts with a specific receptor that is a ligand-gated channel that allows m作者: DEMUR 時間: 2025-3-28 23:34 作者: defendant 時間: 2025-3-29 05:59
https://doi.org/10.1385/1592599494ATP; ATPase; Calcium; Endoplasmatisches Reticulum; Nucleotide; molecular biology; protein; reproducible tec作者: 開始發(fā)作 時間: 2025-3-29 10:48
Calcium Signaling Protocols978-1-59259-949-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Largess 時間: 2025-3-29 14:59
Video-Boom Neue Medien im Vormarsch,An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.作者: curettage 時間: 2025-3-29 19:27
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.作者: 遺產(chǎn) 時間: 2025-3-29 23:26 作者: 微塵 時間: 2025-3-30 03:12
Bernd Schorb,Hans-J?rg Stiehlermulation of receptors, belonging to almost all the classes so far described, will result in changes in [Ca.].. These changes might be directly induced by either Ca.-influx or Ca.-mobilization from intracellular stores, or indirectly by a number of other mechanisms (.–.). The development of fluoresce作者: 燒瓶 時間: 2025-3-30 04:36 作者: Collision 時間: 2025-3-30 12:05
,Eigene Interpretation von § 84 Abs. 2 AMG,r cell parameters, but the usefulness of this approach is limited by several problems, not the least of which is temporal averaging. In population studies, agonists often evoke a characteristic peak and plateau type of response irrespective of the stimulus intensity. However, equivalent experiments 作者: 伸展 時間: 2025-3-30 14:22
,Eigene Interpretation von § 84 Abs. 2 AMG, measurements of intracellular Ca. concentration (.Chapter 7), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications, the primary limitation of FLIPR (i.e., its requirement for single wavelength fluorescent probes using vis作者: Virtues 時間: 2025-3-30 19:11 作者: 令人心醉 時間: 2025-3-31 00:38
https://doi.org/10.1007/978-3-662-08933-0embrane down the steep concentration gradient (.–.). Ca. can enter through two main classes of plasma membrane-located Ca. channels: receptor operated and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransm
