標(biāo)題: Titlebook: CRISPR-Cas Methods; Volume 2 M. Tofazzal Islam,Kutubuddin Ali Molla Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus [打印本頁(yè)] 作者: Madison 時(shí)間: 2025-3-21 17:21
書(shū)目名稱CRISPR-Cas Methods影響因子(影響力)
作者: 戲服 時(shí)間: 2025-3-21 20:18
Chr. Gutenbrunner,G. Hildebrandtew crop variety. This chapter briefly describes the methods of CRISPR-Cas9 vector construction with single and multiple gene targets in rice. The protocol covers all important steps of target sequence selection, single/multiple-target vector construction, mutation detection, and selection of T-DNA free mutant lines.作者: 樂(lè)意 時(shí)間: 2025-3-22 00:36 作者: MAZE 時(shí)間: 2025-3-22 07:02 作者: 下級(jí) 時(shí)間: 2025-3-22 11:36 作者: 墻壁 時(shí)間: 2025-3-22 14:41
In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency,ro method to screen multiple sgRNAs to identify the most suitable one that can efficiently introduce a double-stranded break at a?particular genomic target site. This screening method allows a researcher to choose the best one among several online predicted sgRNAs prior to deliver genome editing reagents into live plant or animal cells.作者: 墻壁 時(shí)間: 2025-3-22 18:34
Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9,o the CRISPR-Cas9-mediated targeted mutagenesis or large fragment deletions in soybean. Detailed procedures will guide through the essential steps including the design of sgRNAs, construction of CRISPR-Cas9 vectors, .-mediated soybean transformation, and identification of mutant lines.作者: minimal 時(shí)間: 2025-3-23 00:39
1949-2448 ation advice from the experts.This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary s作者: 不透氣 時(shí)間: 2025-3-23 02:38
https://doi.org/10.1007/978-3-662-25284-0e, wheat, maize, and tomato, which substantially expands the scope and capabilities of precision plant breeding. Here, we describe a fast and efficient method for construction of prime editing vectors based on Gateway assembly and efficiency assessment of prime editors through transient expression analyses in rice protoplasts.作者: engrave 時(shí)間: 2025-3-23 07:41 作者: Ventricle 時(shí)間: 2025-3-23 11:57
https://doi.org/10.1007/978-3-662-08919-4al applications. Here, we describe the design principle of the microRNA-responsive Cas9 and AcrllA4 (anti-CRISPR protein for SpCas9) switch and the procedures for executing its cell-type-specific genome editing.作者: AUGER 時(shí)間: 2025-3-23 14:13 作者: MONY 時(shí)間: 2025-3-23 18:08 作者: BIAS 時(shí)間: 2025-3-23 22:27
Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9, the . gene induces formation of maternal haploid seeds. Genome editing of all these four genes in hybrid rice simultaneously could fix the heterozygosity and obtain clonal seeds from hybrid rice. Here, we describe a detailed method for generating clonal seeds from hybrid rice by using the multiplex CRISPR-Cas9 technology.作者: Palliation 時(shí)間: 2025-3-24 02:53 作者: Plaque 時(shí)間: 2025-3-24 09:45
An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA That Regulate CRISPR-Cas Systems,RNA ligase 1 (single-stranded RNA ligase) to link two base-paired RNA molecules to investigate potential interaction between sRNA and CRISPR-Cas system. Our goal is to provide readers a detailed method of identifying candidate sRNAs that may regulate CRISPR-Cas adaptation and/or other functions through unbiased screening and validation.作者: 不開(kāi)心 時(shí)間: 2025-3-24 12:19
Naturheilkundliche Pflege von Kindern structure in target recognition and efficacy of CRISPR-Cas systems, it is vital to assess the gRNA secondary structure. Here, we describe a protocol to determine the gRNA secondary structure using RNA-fold software and explain how to interpret the results.作者: 笨重 時(shí)間: 2025-3-24 16:19
Zwischenbilanz und Perspektiven,. Here, we describe a simple method to accurately assemble completely natural, multiplex CRISPR arrays that can be completed in 1–2?days. This should be of great use both in prokaryotes with their own native CRISPR systems and in eukaryotes when paired with Cas12a or other CRISPR nucleases that also process their own arrays.作者: vascular 時(shí)間: 2025-3-24 22:24
Chr. Gutenbrunner,G. Hildebrandtposed of nCas9, a rat cytidine deaminase enzyme APOBEC1, and a uracil DNA glycosylase inhibitor (UGI). The CBE was constructed in the PTF101 vector background. The PTF101-CBE has successfully achieved single-base alteration in soybean. Here, we present a detailed protocol of this base editor to generate a point mutation in soybean.作者: 錯(cuò)事 時(shí)間: 2025-3-25 00:23 作者: 減震 時(shí)間: 2025-3-25 04:14
Jürgen S?keland,Angelika S?kelandbinase polymerase amplification, target gene detection with Cas12a, and lateral flow assay. Owing to its simplicity, efficiency, robustness, and low-cost, this all-paper-based Cas12a DNA test method could be easily applied in field for crop disease diagnosis and GMO test.作者: 艱苦地移動(dòng) 時(shí)間: 2025-3-25 10:59 作者: 民間傳說(shuō) 時(shí)間: 2025-3-25 15:24 作者: GREEN 時(shí)間: 2025-3-25 19:30
In Silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing, structure in target recognition and efficacy of CRISPR-Cas systems, it is vital to assess the gRNA secondary structure. Here, we describe a protocol to determine the gRNA secondary structure using RNA-fold software and explain how to interpret the results.作者: chandel 時(shí)間: 2025-3-25 20:50 作者: PAD416 時(shí)間: 2025-3-26 01:46
Targeted Base Editing in Soybean Using a CRISPR-Cas9 Cytidine Deaminase Fusion,posed of nCas9, a rat cytidine deaminase enzyme APOBEC1, and a uracil DNA glycosylase inhibitor (UGI). The CBE was constructed in the PTF101 vector background. The PTF101-CBE has successfully achieved single-base alteration in soybean. Here, we present a detailed protocol of this base editor to generate a point mutation in soybean.作者: fidelity 時(shí)間: 2025-3-26 05:05
Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato,ovide a detailed protocol for highly efficient plant transformation and regeneration in tomato to generate stable lines along with variant analysis of putative mutant lines followed by phenotypic analysis for the trait-of-interest. From the design of target site, generation of genome-edited tomato plants can be realized in 16–20?weeks.作者: 香料 時(shí)間: 2025-3-26 12:23
CRISPR-Cas12a-Based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants,binase polymerase amplification, target gene detection with Cas12a, and lateral flow assay. Owing to its simplicity, efficiency, robustness, and low-cost, this all-paper-based Cas12a DNA test method could be easily applied in field for crop disease diagnosis and GMO test.作者: Criteria 時(shí)間: 2025-3-26 14:30 作者: 啜泣 時(shí)間: 2025-3-26 18:24 作者: alliance 時(shí)間: 2025-3-26 23:33 作者: 清晰 時(shí)間: 2025-3-27 02:40 作者: GOAD 時(shí)間: 2025-3-27 07:35 作者: OFF 時(shí)間: 2025-3-27 13:05
A Complete Methodology for the Instruction of CRISPR-Based Gene Editing Using a Simplified Cell-Freo system is novel in its ability to present the broad spectrum of gene editing reaction outcomes without the complexity or cost of in vivo cell work. A major variability in inconsistent results arises from the transfection of mammalian cells, often skewing the ratio of successful, precise, and error作者: Obsessed 時(shí)間: 2025-3-27 13:42
Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants,ure precise processing. This multiplex system is highly flexible, allowing researchers to make modifications based on plant species and project objectives. The use of this multiplexing toolbox will broaden the application of CRISPR-Cas12a in basic and translational research in plants.作者: aggrieve 時(shí)間: 2025-3-27 20:17 作者: ADORE 時(shí)間: 2025-3-27 22:27
Designing, Performing, and Analyzing CRISPR-Cas9-Mediated Genome Editing Experiments in Leguminous ning of the CRISPR?constructs, transformation, screening, and analysis of the data. We have also highlighted?different web based tools and resources for assisting genome editing experiments in legumes.作者: 怎樣才咆哮 時(shí)間: 2025-3-28 02:27
1949-2448 organism..The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..978-1-0716-1659-8978-1-0716-1657-4Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: 豐滿有漂亮 時(shí)間: 2025-3-28 08:17 作者: 宣稱 時(shí)間: 2025-3-28 14:19 作者: 焦慮 時(shí)間: 2025-3-28 15:33
https://doi.org/10.1007/978-3-642-87580-9ate the effectiveness and specificity of the construct. Our protocol will make these tools accessible to individuals interested in targeted manipulation of DNA methylation in plants for basic research or crop engineering.作者: 抵制 時(shí)間: 2025-3-28 21:27 作者: 易怒 時(shí)間: 2025-3-28 23:28 作者: maintenance 時(shí)間: 2025-3-29 07:05
In Silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing,acy of the CRISPR-Cas-mediated genome editing are primarily determined by a short sequence known as guide RNA (gRNA). Recent studies have demonstrated that the secondary structure of gRNAs plays a key role in target recognition in CRISPR-Cas-mediated genome editing. Although there are many tools cur作者: 繁榮中國(guó) 時(shí)間: 2025-3-29 08:00
In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency, genomes of interest in a wide variety of organisms. The Cas9 endonuclease enzyme is targeted to a specific genomic region by a small single guide RNA (sgRNA). The cleavage efficiency of Cas9 varies greatly from one sgRNA to another sgRNA. Mutagenesis rate of a?CRISPR-Cas experiment strongly depends作者: bronchodilator 時(shí)間: 2025-3-29 13:30 作者: 憲法沒(méi)有 時(shí)間: 2025-3-29 15:47 作者: 加強(qiáng)防衛(wèi) 時(shí)間: 2025-3-29 23:21
Rapid Assembly of Multiplex Natural CRISPR Arrays,plexing with CRISPR-Cas9 and its homologs presents various technical challenges, such as very long synthetic targeting arrays and time-consuming assembly. Recently, other CRISPR-associated, single-effector nucleases such as Cas12a have been shown to process their own CRISPR arrays, enabling the use 作者: 要素 時(shí)間: 2025-3-30 03:58 作者: WITH 時(shí)間: 2025-3-30 05:42 作者: HEDGE 時(shí)間: 2025-3-30 09:29
Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9,ng. CRISPR-Cas systems can generate highly specific double-strand breaks (DSBs) at the target site, and desired sequence modifications can be introduced during the DSB repair process, such as nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways. Among Cas nuclease proteins, Ca作者: syring 時(shí)間: 2025-3-30 13:33
Targeted Base Editing in Soybean Using a CRISPR-Cas9 Cytidine Deaminase Fusion,ingle-base change or SNP without double-strand break in the genome. Cytosine base editors (CBEs) and adenine base editors (ABEs) are two types of base editors, which are composed of a deactivated Cas9 endonuclease (dCas9) or a Cas9 nickase (nCas9) and a catalytic domain which is capable of deaminati作者: Default 時(shí)間: 2025-3-30 17:15 作者: Left-Atrium 時(shí)間: 2025-3-30 21:52
Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato,-Cas system has become commonplace around the world. This system has been adopted in several plant species. In this protocol, we describe, in a step-wise manner, guidelines for the selection of a suitable vector and target sites, design of sgRNAs, including analysis of off-target activity, construct作者: 倫理學(xué) 時(shí)間: 2025-3-31 02:02 作者: Allege 時(shí)間: 2025-3-31 08:05 作者: BROOK 時(shí)間: 2025-3-31 09:19 作者: 令人心醉 時(shí)間: 2025-3-31 14:31 作者: 夾克怕包裹 時(shí)間: 2025-3-31 20:58
Cell-Type-Specific CRISPR-Cas9 System with miRNAs,e. Regulating Cas9 activity is necessary for the system’s precise genome editing, with editing in only specific cell types being key for future clinical applications. Here, we describe the design principle of the microRNA-responsive Cas9 and AcrllA4 (anti-CRISPR protein for SpCas9) switch and the pr作者: Deceit 時(shí)間: 2025-3-31 21:49
CRISPR/Cas9 Gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-Puro Vectors,l as biotechnology. CRISPR/Cas9 is a form of bacterial defense mechanism that can be used for editing genomes by targeting a 20-nucleotide sequence using a guide RNA and nuclease enzyme called Cas9 enzyme that cleaves target gene. Different protocols have been provided; however, the success of CRISP作者: Ingest 時(shí)間: 2025-4-1 02:05 作者: 有節(jié)制 時(shí)間: 2025-4-1 06:35 作者: 松雞 時(shí)間: 2025-4-1 12:26
Springer Protocols Handbookshttp://image.papertrans.cn/c/image/220558.jpg作者: 發(fā)現(xiàn) 時(shí)間: 2025-4-1 16:09 作者: Foreknowledge 時(shí)間: 2025-4-1 20:54
Inga Mühlenpfordt,Georg Seifertacking for high school, community college, and 4-year college courses. Through funding from a National Science Foundation Advanced Technological Education (NSF-ATE) grant, we have developed an innovative laboratory exercise and curriculum on gene editing. A modular laboratory exercise, based on in v
