標(biāo)題: Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na [打印本頁(yè)] 作者: INEPT 時(shí)間: 2025-3-21 16:16
書(shū)目名稱(chēng)CRISPR-Cas Methods影響因子(影響力)
作者: 熱情贊揚(yáng) 時(shí)間: 2025-3-21 21:45
Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific ExpressioNA double-strand breaks. However, up to now its application was mostly restricted to nonhomologous end-joining-mediated targeted mutagenesis. In contrast, precise genome modifications using a suitable donor sequence for homologous still pose a particular challenge in plants, as NHEJ is the dominant 作者: aesthetic 時(shí)間: 2025-3-22 02:38
Rice Gene Knockout or Downregulation through CRISPR-Cas9, a powerful tool for improving abiotic and biotic stress tolerance and increasing yield in rice. Valuable agronomical traits and functional study of a novel gene can be obtained by targeting single or multiple mutations in the rice genome. Here we describe the sgRNA design, cloning, and assembly of 作者: concentrate 時(shí)間: 2025-3-22 08:05 作者: 拱形大橋 時(shí)間: 2025-3-22 11:42
CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species ,explored in the model grass species . Selection of a single-guide RNA based on location in the gene and low risk of potential off-target effects is initially presented, with the goal of generating INDELS in a single-gene target. Next, the use of the polycistronic t-RNA/gRNA system to simultaneously 作者: AFFIX 時(shí)間: 2025-3-22 16:05 作者: AFFIX 時(shí)間: 2025-3-22 19:48 作者: AMPLE 時(shí)間: 2025-3-22 22:43
An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (,re created mainly by random mutagenesis, which produced many undesirable mutations and genomic rearrangements. With the emergence of programmable site-specific nucleases, mutations can be introduced precisely at specific genomic locations. CRISPR-Cas9 technology represents the most versatile and eff作者: 語(yǔ)源學(xué) 時(shí)間: 2025-3-23 04:21 作者: octogenarian 時(shí)間: 2025-3-23 09:07
Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detectioditing. The recent efforts of scientists allow to improve current genome engineering techniques, which are used to generate modified cell lines for functional studies. Here we describe, in detail, the protocol for DNA-free CRISPR-Cas9-mediated gene editing in a human liver cell line. Briefly, we use作者: COM 時(shí)間: 2025-3-23 10:59 作者: 擋泥板 時(shí)間: 2025-3-23 13:59
Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Elowing for rapid and efficient generation of crop varieties with novel or improved traits. Despite wide applicability of the technology, a challenge in bringing edited crops to market is the lack of regulatory alignment between jurisdictions (e.g., EU). Additionally, technical difficulties in effici作者: 遺棄 時(shí)間: 2025-3-23 18:37
CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol,uable for understanding the function of genes but also offers an efficient approach for genetic manipulation and crop improvement. However, the efficiency of gene editing in wheat (. L.) is highly variable because of the genetic complexity of this plant species including 17 gigabases of genetic code作者: exacerbate 時(shí)間: 2025-3-23 22:46 作者: 圍巾 時(shí)間: 2025-3-24 03:52
Homology-Directed Transgene-Free Gene Editing in ,-based transformation techniques are prohibitively inefficient and lead to predominantly nonhomologous (i.e. off-target) integration. Standard CRISPR-based gene editing protocols have proved too ineffective to enable routine application. We have found that the use of CRISPR-Cpf1 in conjunction with 作者: Indigence 時(shí)間: 2025-3-24 07:59
Der Entwicklungsgang des Menschen,thin a single nucleotide difference to the wild-type sequence) to quickly ascertain the number of bases inserted or deleted on each allele of the targeted gene, which can then be followed by sequencing homozygous progeny of the initial regenerated transformants.作者: 詞匯 時(shí)間: 2025-3-24 14:23 作者: 注意力集中 時(shí)間: 2025-3-24 17:58 作者: THROB 時(shí)間: 2025-3-24 22:51 作者: 設(shè)施 時(shí)間: 2025-3-25 02:30
1949-2448 .aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism..978-1-0716-0618-6978-1-0716-0616-2Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: inculpate 時(shí)間: 2025-3-25 04:33
Correction to: A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome 作者: 我正派 時(shí)間: 2025-3-25 08:03
CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species ,thin a single nucleotide difference to the wild-type sequence) to quickly ascertain the number of bases inserted or deleted on each allele of the targeted gene, which can then be followed by sequencing homozygous progeny of the initial regenerated transformants.作者: 加強(qiáng)防衛(wèi) 時(shí)間: 2025-3-25 11:55
An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (,n a single T-DNA construct via stable .-mediated transformation of immature embryos. Mutations induced at the target loci are detected in T. plants, and homozygous transgene-free mutants segregate in the T. and T. generations. Here, we present methods and strategies for the induction of single and m作者: 切掉 時(shí)間: 2025-3-25 16:26
Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Eer non-transgenic means to genome editing. In this chapter, we describe a novel method for delivery of in vitro purified Cas9/sgRNA complexes into wheat microspores for generation of homozygous edited plants. We provide guidelines for purification of Cas9 protein from bacterial culture, in vitro tra作者: 吹氣 時(shí)間: 2025-3-25 20:21
CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol,protocol for the implementation of CRISPR-mediated wheat gene editing and trait improvement. We begin with gRNA designing using online tools, followed by assembling CRISPR constructs or ribonucleoprotein (RNP) complex and in vitro and in vivo gRNA validation using wheat mesophyll protoplasts. Furthe作者: 音樂(lè)戲劇 時(shí)間: 2025-3-26 03:16
Noch einmal: Geschichte in Schichtgesteinen, proteins) has revolutionized our ability to manipulate DNA and RNA sequences in vitro, ex vivo, and in vivo. In this introductory chapter, we present a brief overview of basics of CRISPR-Cas-mediated genome editing and different orthologues and engineered versions of Cas protein with altered specif作者: GLADE 時(shí)間: 2025-3-26 05:30 作者: Predigest 時(shí)間: 2025-3-26 09:21
Aus der Geschichte der wirbellosen Tiere, a powerful tool for improving abiotic and biotic stress tolerance and increasing yield in rice. Valuable agronomical traits and functional study of a novel gene can be obtained by targeting single or multiple mutations in the rice genome. Here we describe the sgRNA design, cloning, and assembly of 作者: Debark 時(shí)間: 2025-3-26 16:09 作者: 平 時(shí)間: 2025-3-26 20:40 作者: 咆哮 時(shí)間: 2025-3-26 23:13
Die Entfaltung der Wirbeltiere,olecular mechanisms underlying its pathogenicity and broad host range. Gene knockout using homologous recombination has been unviable in oomycetes due to their ploidy (diploidy to polyploidy) and low rates of homologous recombination. CRISPR-mediated genome editing offers a boon for oomycete functio作者: 遭受 時(shí)間: 2025-3-27 03:17 作者: Observe 時(shí)間: 2025-3-27 06:32 作者: saturated-fat 時(shí)間: 2025-3-27 13:01 作者: 墻壁 時(shí)間: 2025-3-27 14:47 作者: 集聚成團(tuán) 時(shí)間: 2025-3-27 21:38
Die Entfaltung der Wirbeltiere, developed as an effective genome-editing tool. It is used for targeted mutagenesis, whereby Cas9 enzyme creates a DNA double-strand break (DSB), which is then repaired by mutation-prone non-homologous end joining (NHEJ) repair system; consequently, the resulting DNA sequence collects mutations eith作者: 顯示 時(shí)間: 2025-3-27 23:04 作者: 大罵 時(shí)間: 2025-3-28 05:39
Der Entwicklungsgang des Menschen,uable for understanding the function of genes but also offers an efficient approach for genetic manipulation and crop improvement. However, the efficiency of gene editing in wheat (. L.) is highly variable because of the genetic complexity of this plant species including 17 gigabases of genetic code作者: motor-unit 時(shí)間: 2025-3-28 06:35 作者: Volatile-Oils 時(shí)間: 2025-3-28 13:28 作者: 吹氣 時(shí)間: 2025-3-28 17:19 作者: Injunction 時(shí)間: 2025-3-28 20:34
978-1-0716-0618-6Springer Science+Business Media, LLC, part of Springer Nature 2020作者: 裹住 時(shí)間: 2025-3-28 22:55
CRISPR-Cas Methods978-1-0716-0616-2Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: 乞丐 時(shí)間: 2025-3-29 07:02 作者: obligation 時(shí)間: 2025-3-29 08:26 作者: 巨碩 時(shí)間: 2025-3-29 13:26 作者: 消極詞匯 時(shí)間: 2025-3-29 18:26 作者: SKIFF 時(shí)間: 2025-3-29 20:55 作者: Project 時(shí)間: 2025-3-30 02:38 作者: Infant 時(shí)間: 2025-3-30 06:44 作者: 小蟲(chóng) 時(shí)間: 2025-3-30 08:56
Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expressioependent of high transformation efficiencies. Here, we describe an updated protocol of ipGT for the model plant Arabidopsis, taking into account our recent improvements based on egg-cell-specific expression of . Cas9.作者: 教唆 時(shí)間: 2025-3-30 14:04 作者: pulmonary 時(shí)間: 2025-3-30 16:53
Homology-Directed Transgene-Free Gene Editing in ,possibility to purchase all necessary reagents commercially with no preparation time (besides design) facilitates rapid and routine genetic engineering in this organism. Here we describe the use of this technique to knockout locus ., which leads to rapamycin resistance and lends itself to an easy assay when adopting this gene-editing protocol.作者: Mingle 時(shí)間: 2025-3-31 00:05 作者: 傳授知識(shí) 時(shí)間: 2025-3-31 03:52 作者: MORPH 時(shí)間: 2025-3-31 07:13
Die Entfaltung der Wirbeltiere, system over other transformation methods. In this chapter, we describe a detailed protocol for generating . mutants via AMT to deliver single-guide RNA and Cas9, the components required for gene editing. This protocol can be used directly for CRISPR-Cas9-mediated gene editing of . and modified for other culturable oomycete species.
