標(biāo)題: Titlebook: CRISPR Gene Editing; Methods and Protocol Yonglun Luo Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR-C [打印本頁] 作者: 導(dǎo)彈 時間: 2025-3-21 19:57
書目名稱CRISPR Gene Editing影響因子(影響力)
書目名稱CRISPR Gene Editing影響因子(影響力)學(xué)科排名
書目名稱CRISPR Gene Editing網(wǎng)絡(luò)公開度
書目名稱CRISPR Gene Editing網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱CRISPR Gene Editing被引頻次
書目名稱CRISPR Gene Editing被引頻次學(xué)科排名
書目名稱CRISPR Gene Editing年度引用
書目名稱CRISPR Gene Editing年度引用學(xué)科排名
書目名稱CRISPR Gene Editing讀者反饋
書目名稱CRISPR Gene Editing讀者反饋學(xué)科排名
作者: Mortal 時間: 2025-3-21 20:43 作者: 死亡率 時間: 2025-3-22 01:13
James Corner,Gilles A. Tiberghientable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.作者: 馬具 時間: 2025-3-22 06:21
Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAsreptococcus pyogenes complexed with chemically modified sgRNAs. We have found this protocol to work very efficiently in numerous cell lines and primary cells that are difficult to transfect by conventional chemical-based transfection methods.作者: BUST 時間: 2025-3-22 12:08
CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cellstable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.作者: 軟膏 時間: 2025-3-22 13:19
Book 2019 target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included作者: 軟膏 時間: 2025-3-22 20:40
CRISPR-gRNA Designapter. The gRNA targets the genome site to be edited, giving great importance to its design to obtain increased efficiency and decreased off-target events. In this chapter, we describe different tools to design suitable gRNAs for a variety of experimental purposes.作者: 借喻 時間: 2025-3-22 23:54
Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery protein and single guide RNA (sgRNA), the key components of the CRISPR genome editing system. Here, we provide a protocol for production and validation of VSV-G-pseudotyped lentiviral vectors for delivery of the CRISPR system and generation of knockout cell lines.作者: Melodrama 時間: 2025-3-23 02:29
Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9iciency. This chapter covers protocols for the preparation of reagents to target loci of interest, the transfection, and for the genotyping of single cell-derived iPSC clones. Furthermore, we provide a protocol for the convenient generation of plasmids enabling multiplex gene targeting.作者: Barter 時間: 2025-3-23 07:39
Contemporary Urban Design Thinkinglow transfection efficiency by conventional transfection method. In this chapter, what we present is an easy method by fabricating CRISPR-Cas9 plasmids into nanoparticle system and efficiently delivered into target cells to achieve gene editing.作者: Conspiracy 時間: 2025-3-23 10:43
Methodologischer Hintergrund der Arbeit,netic engineering of the . genomic safe harbor. When implemented in human pluripotent stem cells (hPSCs), the approach can be then efficiently applied to virtually any hPSC-derived human cell type at various stages of development or disease.作者: 小卒 時間: 2025-3-23 15:49 作者: 死亡率 時間: 2025-3-23 18:58 作者: ORBIT 時間: 2025-3-24 00:50
Book 2019oritative and invaluable, .CRISPR Gene Editing: Methods and Protocols. will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.?.Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com..作者: CRUE 時間: 2025-3-24 03:31 作者: APRON 時間: 2025-3-24 09:16 作者: 容易生皺紋 時間: 2025-3-24 14:18 作者: 群居動物 時間: 2025-3-24 18:02
Aida Bani,Dolja Pavlova,Seit Shallariintroducing DSBs, known as CRISPR’s DNA-binding footprints. The indel tracking methods have contributed greatly to the improvement of CRISPR-Cas9 activity and specificity. Here, we review and discuss strategies developed over that past few years to track the CRISPR’s footprints, their advantages, and limitations.作者: DAUNT 時間: 2025-3-24 19:13
Godmother of Sustainable Development,Here we describe our method for rapid and efficient generation of gene knockout or deletion cells using CRISPR/Cas9 within the time span of one month. The design of gRNAs, plasmid cloning, transfection, cell culturing, positive clone selection, and screening can be obtained from this method.作者: 特別容易碎 時間: 2025-3-24 23:20
Tracking CRISPR’s Footprintsintroducing DSBs, known as CRISPR’s DNA-binding footprints. The indel tracking methods have contributed greatly to the improvement of CRISPR-Cas9 activity and specificity. Here, we review and discuss strategies developed over that past few years to track the CRISPR’s footprints, their advantages, and limitations.作者: 歡樂中國 時間: 2025-3-25 07:13
Rapid and Efficient Gene Deletion by CRISPR/Cas9Here we describe our method for rapid and efficient generation of gene knockout or deletion cells using CRISPR/Cas9 within the time span of one month. The design of gRNAs, plasmid cloning, transfection, cell culturing, positive clone selection, and screening can be obtained from this method.作者: fastness 時間: 2025-3-25 07:51
Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDERorithm (available at . or .). The routine is easy, fast, and provides much more detailed information than current enzyme-based assays. TIDE and TIDER accelerate testing and designing of DSB-based genome editing strategies.作者: 無節(jié)奏 時間: 2025-3-25 14:21
Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAf CRISPR/Cas9 KO and repurposed systems. To this end, validation of gRNA functionality depends on efficient, accurate, and sensitive identification of indels induced by a given gRNA design. For in vitro indel profiling the most commonly used methods are based on amplicon size discrimination or seque作者: 離開就切除 時間: 2025-3-25 18:58
Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Checktivity, as well as for enrichment of gene edited cells. In this chapter, we will give a step-by-step instruction on the design, generation, and application of the C-Check system for quantifying gRNA activities.作者: Deceit 時間: 2025-3-25 20:26
Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Virspecificities. Here, we describe a fast and simple protocol for small-scale packaging of CRISPR/Cas9 components into AAV vectors. To showcase its potential, we employ this method for screening of gRNAs targeting the murine miR-122 locus in Hepa1–6 cells (using AAV serotype 6, AAV6) or the 5′LTR of t作者: prediabetes 時間: 2025-3-26 02:19 作者: PAGAN 時間: 2025-3-26 05:28 作者: 逃避系列單詞 時間: 2025-3-26 08:56 作者: 節(jié)約 時間: 2025-3-26 13:22 作者: interior 時間: 2025-3-26 18:06
https://doi.org/10.1007/978-3-030-89525-9specificities. Here, we describe a fast and simple protocol for small-scale packaging of CRISPR/Cas9 components into AAV vectors. To showcase its potential, we employ this method for screening of gRNAs targeting the murine miR-122 locus in Hepa1–6 cells (using AAV serotype 6, AAV6) or the 5′LTR of t作者: NICE 時間: 2025-3-26 23:42
https://doi.org/10.1007/978-3-658-37228-6d clones. We also present our experiences of generating single nucleotide changes at 15 sites, which show considerable variability between both guides and target sites in the efficiency at which such changes can be introduced.作者: 同來核對 時間: 2025-3-27 02:23
1064-3745 idelines and methods for the vitally important CRISPR gene editing field.?.Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com..978-1-4939-9170-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: incarcerate 時間: 2025-3-27 06:10 作者: 現(xiàn)暈光 時間: 2025-3-27 11:37 作者: observatory 時間: 2025-3-27 16:28
Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER mutations by the genome editing method are largely dependent on the target site. As a consequence, the outcome of the editing operation is difficult to predict. Therefore, a quick assay to quantify the frequency of mutations is vital for a proper assessment of genome editing actions. We developed t作者: Albumin 時間: 2025-3-27 21:43
Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAls, tissues, and whole animals showing great promise for translational applications. With regard to CRISPR/Cas9, a variety of repurposed systems have been developed to achieve gene knock-out, base editing, targeted knock-in, gene activation/repression, epigenetic modulation, and locus-specific label作者: 潛移默化 時間: 2025-3-28 01:21 作者: fertilizer 時間: 2025-3-28 03:18 作者: 紀(jì)念 時間: 2025-3-28 09:09 作者: 真 時間: 2025-3-28 12:58
Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Virntal biological questions and to target and treat genetic diseases. The CRISPR system, originally derived from bacteria and archaea, can be delivered into cells using different techniques, comprising (1) transfection of mRNA or plasmid DNA, (2) electroporation of plasmid DNA or the Cas9 protein in a作者: 密碼 時間: 2025-3-28 16:11 作者: 走調(diào) 時間: 2025-3-28 22:11 作者: Nucleate 時間: 2025-3-29 00:56 作者: 改變 時間: 2025-3-29 06:54
Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9ut experiments using CRISPR/Cas9 are very valuable, these are not well suited to study stage-specific gene function in dynamic situations such as development or disease. Here we describe a CRISPR/Cas9-based OPTimized inducible gene KnockOut method (OPTiKO) for conditional loss-of-function studies in作者: 挖掘 時間: 2025-3-29 10:14
CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. H作者: 迅速飛過 時間: 2025-3-29 14:47 作者: Nebulizer 時間: 2025-3-29 16:59
Yonglun LuoIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: happiness 時間: 2025-3-29 22:55
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220555.jpg作者: indignant 時間: 2025-3-30 01:09 作者: 原始 時間: 2025-3-30 05:35 作者: 最低點(diǎn) 時間: 2025-3-30 10:40
https://doi.org/10.5822/978-1-61091-797-1Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.作者: Obscure 時間: 2025-3-30 15:25
Genome Editing in MiceProgrammable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.作者: catagen 時間: 2025-3-30 18:15
Studies in Computational Intelligence(CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool. To fulfill CRISPR experiment’s goals, two components are important: an endonuclease and a gRNA. The most commonly used endonucleases are Cpf1 and Cas9 and are described in depth in this ch作者: Psychogenic 時間: 2025-3-31 00:34 作者: 思考 時間: 2025-3-31 04:07
Shalini Dhyani,Anil Kumar Gupta,Madhav Karki mutations by the genome editing method are largely dependent on the target site. As a consequence, the outcome of the editing operation is difficult to predict. Therefore, a quick assay to quantify the frequency of mutations is vital for a proper assessment of genome editing actions. We developed t作者: Galactogogue 時間: 2025-3-31 07:39
https://doi.org/10.1007/978-981-15-4712-6ls, tissues, and whole animals showing great promise for translational applications. With regard to CRISPR/Cas9, a variety of repurposed systems have been developed to achieve gene knock-out, base editing, targeted knock-in, gene activation/repression, epigenetic modulation, and locus-specific label
