標(biāo)題: Titlebook: CRISPR; Methods and Protocol Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fi Book 2015 Springer Science+Business Media New York 2015 CRI [打印本頁] 作者: 方面 時間: 2025-3-21 19:40
書目名稱CRISPR影響因子(影響力)
書目名稱CRISPR影響因子(影響力)學(xué)科排名
書目名稱CRISPR網(wǎng)絡(luò)公開度
書目名稱CRISPR網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱CRISPR被引頻次
書目名稱CRISPR被引頻次學(xué)科排名
書目名稱CRISPR年度引用
書目名稱CRISPR年度引用學(xué)科排名
書目名稱CRISPR讀者反饋
書目名稱CRISPR讀者反饋學(xué)科排名
作者: misanthrope 時間: 2025-3-21 20:29 作者: Musket 時間: 2025-3-22 01:11 作者: GRAZE 時間: 2025-3-22 07:31 作者: 休戰(zhàn) 時間: 2025-3-22 09:47
Samiran Bera,Santosh Kumar Das,Joydev Ghoshs a metal-dependent endonuclease activity. Here we describe the protocols for the analysis of nuclease activity of purified Cas1 proteins against various DNA substrates including Holliday junctions and other intermediates of DNA recombination and repair.作者: 吊胃口 時間: 2025-3-22 14:37 作者: 吊胃口 時間: 2025-3-22 18:10
In Vitro Co-reconstitution of Cas Protein Complexes,roduced as inactive inclusion bodies. Here, we present a detailed protocol for the isolation and purification of insoluble Cas proteins. Guidelines for their solubilization via co-reconstitution strategies and procedures to upscale the production of soluble multimeric Cas protein complexes are provided.作者: 種屬關(guān)系 時間: 2025-3-22 23:56
Rapid Multiplex Creation of , Strains Capable of Interfering with Phage Infection Through CRISPR,,” can be used for very simple and rapid construction of multiple . strains capable of targeting, through CRISPR interference, any phage or plasmid of interest. Availability of such strains should allow rapid progress in the analysis of CRISPR-Cas system function against diverse mobile genetic elements.作者: 暗語 時間: 2025-3-23 03:18
Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes,es of crRNA-Cas complexes can be quantified by calculating the dissociation constant (..). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from . as an example.作者: 社團(tuán) 時間: 2025-3-23 09:37 作者: 圣歌 時間: 2025-3-23 10:50 作者: 愛國者 時間: 2025-3-23 16:56
Book 2015chemical and structural levels.?.CRISPR: Methods and Protocols.?guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from?standard protocols of DNA, RNA and protein biology.?Written in the highly successful?.Methods in Molecula作者: foliage 時間: 2025-3-23 19:01 作者: Morose 時間: 2025-3-23 23:48
Jesus Soto,Patricia Melin,Oscar Castillots in DNA degradation. Here, we describe assays for monitoring of St-Cas3 nuclease, ATPase and helicase activities in a stand-alone form and in the presence of the Cascade ribonucleoprotein complex. These assays can be easily adapted for biochemical analysis of Cas3 proteins from different microorganisms.作者: 有常識 時間: 2025-3-24 05:23
Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS) performed using RNase digestion in conjunction with liquid chromatography tandem MS analysis. Using the intact mass of the crRNA, in conjunction with RNase mapping experiments enabled the identification and characterisation of the crRNA, providing further insight into crRNA processing in a number of type I CRISPR-Cas systems.作者: 小淡水魚 時間: 2025-3-24 07:34 作者: 隱藏 時間: 2025-3-24 13:09 作者: 制度 時間: 2025-3-24 17:44
Computational Detection of CRISPR/crRNA Targets,s have a discriminatory role in accurate target detection. Here, we describe a bioinformatic method, called CRISPRTarget, to use the sequence of a CRISPR array (e.g., predicted via CRISPRDetect/CRISPRDirection) to identify the foreign nucleic acids it targets.作者: ablate 時間: 2025-3-24 20:56
Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification of Target Intompared to those obtained for a reference molecule in independent experiments. Here we describe the use of a transforming mixture of plasmids that includes the non-targeted vector as an internal reference to obtain normalized data which are unbiased by empirical variations.作者: Progesterone 時間: 2025-3-25 02:43
Expression and Purification of the CMR (Type III-B) Complex in ,,xes, which degrade invading nucleic acids in a sequence homology-dependent manner in many prokaryotic species. Here, we describe the expression of a tandem-tagged subunit of the Type III-B (CMR) complex in . and subsequent isolation and purification of the whole complex by affinity purification of the tagged subunit.作者: nepotism 時間: 2025-3-25 03:53 作者: Abrupt 時間: 2025-3-25 09:58 作者: Hallmark 時間: 2025-3-25 12:41
Studies in Computational IntelligenceO.) and the specific cleavage of single-stranded DNA by Nuclease P1 can be used to probe an R-loop formation by Cascade. Localization of the Cascade-crRNA complex on the DNA can be achieved by an Exonuclease III protection assay.作者: 諷刺 時間: 2025-3-25 17:44 作者: 搬運工 時間: 2025-3-25 21:11 作者: 高射炮 時間: 2025-3-26 01:41
https://doi.org/10.1007/978-3-319-13650-9ucts. The mechanism of cleavage is investigated by chemical and enzymatic characterization of the reaction products as well as by the demonstration that a specific 2′-deoxy substitution 5′ to the scissile phosphate blocks endonucleolytic cleavage.作者: reject 時間: 2025-3-26 06:50
Yang Liu,Alan Kwan,Yacine Rezgui,Haijiang Lis have a discriminatory role in accurate target detection. Here, we describe a bioinformatic method, called CRISPRTarget, to use the sequence of a CRISPR array (e.g., predicted via CRISPRDetect/CRISPRDirection) to identify the foreign nucleic acids it targets.作者: mydriatic 時間: 2025-3-26 09:07 作者: 明智的人 時間: 2025-3-26 16:27
José M. Araújo,Carlos E. T. Dóreaxes, which degrade invading nucleic acids in a sequence homology-dependent manner in many prokaryotic species. Here, we describe the expression of a tandem-tagged subunit of the Type III-B (CMR) complex in . and subsequent isolation and purification of the whole complex by affinity purification of the tagged subunit.作者: bifurcate 時間: 2025-3-26 18:39 作者: 馬籠頭 時間: 2025-3-26 22:10 作者: miscreant 時間: 2025-3-27 03:29
Adis Alihodzic,Eva Tuba,Milan Tubas for each of which signature genes have been identified. Comparative genomic analysis of the CRISPR-Cas systems in new archaeal and bacterial genomes performed over the 3 years elapsed since the development of this classification makes it clear that new types and subtypes of CRISPR-Cas need to be i作者: neutrophils 時間: 2025-3-27 06:38 作者: 不連貫 時間: 2025-3-27 13:04 作者: Anhydrous 時間: 2025-3-27 15:08
Nabil Sabor,Mohammed Abo-Zahhad, suspected as required for this lethal activity. This procedure enabled us to identify a novel gene, ., that is required for the activity of the CRISPR-Cas system. The procedures described here can be adjusted to various organisms to identify genes required for their CRISPR-Cas activity.作者: heterogeneous 時間: 2025-3-27 21:39
Investigating CRISPR RNA Biogenesis and Function Using RNA-seq, a genome-wide annotation of transcriptional start sites. This approach helped to identify a new crRNA biogenesis pathway of Type II CRISPR-Cas systems that involves a trans-encoded small RNA, tracrRNA, and the host factor RNase III.作者: 擴(kuò)張 時間: 2025-3-27 23:06 作者: indubitable 時間: 2025-3-28 05:15
Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids,as the model system in the first published study on novel spacer acquisition and in many studies ever since, the protocols in this chapter describe specific conditions, media, and buffers that have been used with this microorganism. Details for other species will be given when possible, but readers 作者: 清楚 時間: 2025-3-28 09:02 作者: 使顯得不重要 時間: 2025-3-28 11:01
Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function,, suspected as required for this lethal activity. This procedure enabled us to identify a novel gene, ., that is required for the activity of the CRISPR-Cas system. The procedures described here can be adjusted to various organisms to identify genes required for their CRISPR-Cas activity.作者: Oscillate 時間: 2025-3-28 15:23 作者: 拱形面包 時間: 2025-3-28 20:39
In Vitro Co-reconstitution of Cas Protein Complexes,n of the mechanistic details and the protein interaction partners requires production of recombinant Cas proteins. However, these proteins are often produced as inactive inclusion bodies. Here, we present a detailed protocol for the isolation and purification of insoluble Cas proteins. Guidelines fo作者: bifurcate 時間: 2025-3-29 00:31
Analysis of CRISPR Pre-crRNA Cleavage,Cas system by incubation of radiolabeled model RNAs with recombinant CRISPR-associated (Cas) endoribonucleases, followed by denaturing polyacrylamide gel electrophoresis (PAGE) of the products. Determination of cleavage position is based on comparison with RNase T1 digestion and base hydrolysis prod作者: osteopath 時間: 2025-3-29 05:25 作者: indecipherable 時間: 2025-3-29 10:44
Computational Detection of CRISPR/crRNA Targets,ys specifically determines the targets in invader genomes. These spacers provide the short specific RNA nucleotide sequences within the guide crRNAs. In addition to complementarity in the spacer–target (protospacer) interaction, short flanking protospacer adjacent motifs (PAMs), or mismatching flank作者: 生氣的邊緣 時間: 2025-3-29 11:35
High-Throughput CRISPR Typing of , Complex and , Serotype Typhimurium,inical isolates at a phylogeographic level. For other pathogens, such as ., recent studies suggest that specifically designed spoligotyping techniques could be interesting for public health purposes. Spoligotyping was in its original format a reverse line-blot hybridization method using capture prob作者: tariff 時間: 2025-3-29 16:22
Spacer-Based Macroarrays for CRISPR Genotyping, loci in bacteria. To date, it was used almost exclusively for . and was named spoligotyping (spacer oligonucleotides typing). Here, we describe the pipeline of this approach that includes search of loci and selection of spacers, preparation of the membrane with immobilized probes and spoligotyping 作者: 細(xì)胞 時間: 2025-3-29 21:36
Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS)id chromatography interfaced with electrospray ionization mass spectrometry (LC ESI MS). The direct purification of crRNA from the Cascade-crRNA complex was performed using denaturing ion pair reverse phase chromatography. Following purification of the crRNA, the intact mass was determined by LC ESI作者: 熱心 時間: 2025-3-30 00:28 作者: companion 時間: 2025-3-30 08:03 作者: BLANK 時間: 2025-3-30 09:18 作者: 該得 時間: 2025-3-30 13:27
Expression and Purification of the CMR (Type III-B) Complex in ,,n or a multi-protein complex. However, expression and purification of one subunit of a complex in the absence of its binding partners has often proven difficult to achieve due to the issues such as instability and mis-folding. This is the case for the components of the CRISPR-Cas interference comple作者: FIR 時間: 2025-3-30 16:32
Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids,lution. The first main step of CRISPR-Cas activity is the immune adaptation through spacer(s) acquisition into an active CRISPR locus. This step is also mandatory for the final stage of CRISPR-Cas activity, namely interference. This chapter describes general procedures for studying the CRISPR adapta作者: gustation 時間: 2025-3-30 23:18 作者: BRAND 時間: 2025-3-31 02:33 作者: 罐里有戒指 時間: 2025-3-31 05:57 作者: sperse 時間: 2025-3-31 09:30 作者: 機密 時間: 2025-3-31 16:29 作者: fatty-streak 時間: 2025-3-31 17:43
Chemical and Enzymatic Footprint Analyses of R-Loop Formation by Cascade-crRNA Complex,DNA-loop) at the crRNA-complementary sequence (protospacer). After initial unspecific binding to the double-stranded DNA, Cascade-crRNA complex slides along the DNA to find the protospacer. Once the target site is detected, the crRNA hybridizes to the complementary strand with subsequent displacemen作者: characteristic 時間: 2025-3-31 23:23 作者: 騙子 時間: 2025-4-1 02:11
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220552.jpg作者: infelicitous 時間: 2025-4-1 08:34 作者: 偽造 時間: 2025-4-1 13:29 作者: 劇毒 時間: 2025-4-1 14:19
