作者: 造反,叛亂 時(shí)間: 2025-3-21 22:15 作者: zonules 時(shí)間: 2025-3-22 01:47
Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8,ation is an elegant and efficient procedure to investigate endogenous protein interactions or complexes. Provided that the targeted complex is soluble in mild detergent these complexes can be recovered using protein A/G-coupled Sepharose beads and further analyzed after denaturation and electrophore作者: 字的誤用 時(shí)間: 2025-3-22 06:33
In Vitro Evaluation of the Apoptosis Function in Human Activated T Cells, the intrinsic pathway on in vitro activated T cells. Her, we describe an in vitro assay allowing the monitoring of three different apoptosis pathways: (1) the FAS-induced pathway, (2) the activation-induced cell death (AICD), and (3) the death induced by starvation of the cells, called activated-ce作者: jabber 時(shí)間: 2025-3-22 11:51
Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition,c signal at the detriment of the non-apoptotic signaling pathway [1–6]. Also CD95 can form different protein complexes via dynamic protein–protein interactions (PPIs) according to its interaction with soluble or transmembrane CD95L. Therefore, spatiotemporal identification of these PPIs is pivotal t作者: 不朽中國(guó) 時(shí)間: 2025-3-22 14:44 作者: 不朽中國(guó) 時(shí)間: 2025-3-22 20:05 作者: armistice 時(shí)間: 2025-3-22 23:02
CD95-Mediated Calcium Signaling,r calcium signals that control the cell fate, apoptosis, or survival, depending on the ligand (membrane or soluble). Intracellular calcium determination is an exquisite readout to explore the molecular mechanisms elicited by CD95 engagement. The most widely applied methods for studying calcium signa作者: Tartar 時(shí)間: 2025-3-23 02:35 作者: 南極 時(shí)間: 2025-3-23 09:18
Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K,ence 245(4915):301–305, 1989). Accumulating evidence indicate that this so-called death receptor can also trigger non-apoptotic signaling pathways promoting inflammation and oncogenesis (Barnhart et al., Embo J 23(15):3175–3185, 2004; Chen et al., Nature 465(7297):492-496, 2010; Legembre et al., Cel作者: 委托 時(shí)間: 2025-3-23 13:28
Organelle Separation and Cell Signaling, for the signal transduction. For instance, ubiquitylated components of the NF-κB pathway accumulated at the endoplasmic reticulum while ubiquitylated components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components作者: 軍火 時(shí)間: 2025-3-23 17:21 作者: 不妥協(xié) 時(shí)間: 2025-3-23 21:19
Isolation of Lipid Rafts Through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Rce to detergent solubilization. Despite rafts have been involved in survival processes, these membrane domains have also been shown to play a major role in the modulation of death receptor signaling. Here, we describe a detailed protocol for isolating lipid rafts from whole cells by taking advantage作者: 課程 時(shí)間: 2025-3-24 00:07
Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Micro physiological conditions. It can also be used to measure the forces and mechanics from single molecule interaction to cell–cell adhesion. Here, we present a methodology that allows to quantify cell elastic properties (using the Young modulus) and cell membrane tension modulated by CD95/cl-CD95L int作者: Aesthete 時(shí)間: 2025-3-24 03:51
Sketching of CD95 Oligomers by In Silico Investigations,e been able to obtain a consensual organization of the complex. Our strategy permitted the construction of a plausible trimer, and to sketch the interface between protomers. The final model will guide further experimental investigations and understanding of CD95 structure and functions.作者: Adj異類(lèi)的 時(shí)間: 2025-3-24 10:28
Site-Specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventionaival signals. Recently, site-specific monoclonal antibodies against phosphorylated tyrosines of CD95 have been generated and used to successfully detect each phosphorylated death domain tyrosine of CD95 directly and separately by immunoblotting. Here we provide detailed protocols and useful tips for作者: CLAY 時(shí)間: 2025-3-24 12:33 作者: Classify 時(shí)間: 2025-3-24 18:21
Exploration of Fas S-Nitrosylation by the Biotin Switch Assay,ted in cancer cell lines by NO donors or iNOS activation. This posttranslational modification (PTM) induces Fas aggregation into lipid rafts and enhances FasL-mediated signaling and apoptosis. In this report, we describe the detection of Fas S-nitrosylation by the most commonly used method, the biot作者: 繼承人 時(shí)間: 2025-3-24 19:32 作者: 合乎習(xí)俗 時(shí)間: 2025-3-25 00:06
Book 2017 biochemical, cellular methods and animal models?to in order to better understand the biological functions of this cytokine. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and 作者: Thyroiditis 時(shí)間: 2025-3-25 03:30
https://doi.org/10.1007/978-3-322-95767-2es on the ability of ascorbate to reduce the covalent bond between the NO radical and the protein, allowing the exchange of the NO radical with a thiol reactive biotin-HPDP. The biotinylated proteins are then easily purified by using NeutrAvidin resin, separated by SDS-PAGE resolution and analyzed by Western blotting.作者: CROW 時(shí)間: 2025-3-25 11:11
Exploration of Fas S-Nitrosylation by the Biotin Switch Assay,es on the ability of ascorbate to reduce the covalent bond between the NO radical and the protein, allowing the exchange of the NO radical with a thiol reactive biotin-HPDP. The biotinylated proteins are then easily purified by using NeutrAvidin resin, separated by SDS-PAGE resolution and analyzed by Western blotting.作者: intercede 時(shí)間: 2025-3-25 14:35
Thomas Koszinowski,Hanspeter Mattestic separation by western blotting or mass spectrometry. Herein, we describe in detail the method used in our laboratory to immunoprecipitate and analyze by immunoblot complexes containing caspase-8, using a commercial antibody directed against caspase-8.作者: Prophylaxis 時(shí)間: 2025-3-25 16:23 作者: concise 時(shí)間: 2025-3-25 23:50 作者: BURSA 時(shí)間: 2025-3-26 03:34 作者: 補(bǔ)充 時(shí)間: 2025-3-26 07:19 作者: 路標(biāo) 時(shí)間: 2025-3-26 09:53 作者: 實(shí)施生效 時(shí)間: 2025-3-26 16:07 作者: Ingenuity 時(shí)間: 2025-3-26 19:40
Thomas Koszinowski,Hanspeter Mattes components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays.作者: 牛的細(xì)微差別 時(shí)間: 2025-3-26 23:29 作者: POLYP 時(shí)間: 2025-3-27 04:11 作者: V洗浴 時(shí)間: 2025-3-27 06:57
CD95-Mediated Calcium Signaling,on is an exquisite readout to explore the molecular mechanisms elicited by CD95 engagement. The most widely applied methods for studying calcium signaling pathways use fluorescent indicators and imaging methods with fluorescence microscopy. This technical approach, however, requires many precautions that we discuss in this chapter.作者: Tincture 時(shí)間: 2025-3-27 13:24 作者: 貪婪性 時(shí)間: 2025-3-27 14:20 作者: 比賽用背帶 時(shí)間: 2025-3-27 18:20
Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microesent a methodology that allows to quantify cell elastic properties (using the Young modulus) and cell membrane tension modulated by CD95/cl-CD95L interactions by coupling nanoindentation and membrane tube pulling using suitably decorated AFM levers.作者: 圍裙 時(shí)間: 2025-3-28 00:29 作者: PLIC 時(shí)間: 2025-3-28 02:25
Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8,tic separation by western blotting or mass spectrometry. Herein, we describe in detail the method used in our laboratory to immunoprecipitate and analyze by immunoblot complexes containing caspase-8, using a commercial antibody directed against caspase-8.作者: PHONE 時(shí)間: 2025-3-28 10:03
Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization Induced by CD95 Activattablished protocol using classical fluorescent probes, DIOC.(3) and TMRM. Living cells are loaded with these cationic dyes, which accumulate in mitochondria. After CD95 activation, organelle depolarization is assessed using flow cytometry.作者: 運(yùn)氣 時(shí)間: 2025-3-28 10:55
Isolation of Lipid Rafts Through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death R of the lipid raft resistance to Triton X-100 solubilization at 4 °C, followed by sucrose gradient centrifugation, with subsequent analysis of Fas/CD95 death receptor localization in the raft fractions by immunoblotting. This method is also useful to localize additional proteins in membrane rafts.作者: MAG 時(shí)間: 2025-3-28 17:47 作者: HARP 時(shí)間: 2025-3-28 20:25 作者: 中世紀(jì) 時(shí)間: 2025-3-28 23:54 作者: NAIVE 時(shí)間: 2025-3-29 06:54 作者: Mortar 時(shí)間: 2025-3-29 07:45
Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L, erythematosus by promoting the accumulation of activated T lymphocytes in enflamed organs (Tauzin et al., PLoS Biol 9:e1001090, 2011). This chapter aims at describing the methodology used to measure the chemoattractive effect of cl-CD95L on human cancer cells and lymphocytes.作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-29 13:41 作者: Ligneous 時(shí)間: 2025-3-29 16:45 作者: frivolous 時(shí)間: 2025-3-29 21:30 作者: 禁止 時(shí)間: 2025-3-30 02:54 作者: 臭了生氣 時(shí)間: 2025-3-30 07:41 作者: 全神貫注于 時(shí)間: 2025-3-30 10:58
https://doi.org/10.1007/978-1-4939-6780-3Cell motility; Immunoprecipitation; TNF Receptor; apoptosis; pathophysiology作者: 事先無(wú)準(zhǔn)備 時(shí)間: 2025-3-30 15:27 作者: CUR 時(shí)間: 2025-3-30 17:13 作者: 多產(chǎn)魚(yú) 時(shí)間: 2025-3-30 22:05 作者: Ornament 時(shí)間: 2025-3-31 04:32
https://doi.org/10.1007/978-3-322-95556-2g. 1a). This membrane-bound cytokine is mainly expressed at the surface of activated T lymphocytes and natural killer cells, where it is used as an apoptotic factor to eliminate infected and transformed cells (Strasser et al., Immunity 30:180–192, 2009).作者: custody 時(shí)間: 2025-3-31 07:42
Die politische Entwicklung 1989 im überblickce apoptosis in cancer and virus-infected cells. The goal of this chapter is to describe a method used to immunoprecipitate CD95 and analyze its associated protein complex in cells stimulated with a cytotoxic CD95L (i.e., Ig-CD95L).作者: 極小量 時(shí)間: 2025-3-31 10:12 作者: 小卒 時(shí)間: 2025-3-31 17:19 作者: 飛來(lái)飛去真休 時(shí)間: 2025-3-31 19:14 作者: 樣式 時(shí)間: 2025-4-1 00:14
Die politische Entwicklung 1990 im überblickof crucial interest to monitor the CD95-mediated apoptotic signal. In this chapter, we report how we evaluate the drop of the mitochondrial transmembrane potential in leukemic cells and adherent triple negative breast cancer cells exposed to cytotoxic CD95L. We describe a simple, robust, and well-es
