標(biāo)題: Titlebook: C. elegans; Methods and Applicat Kevin Strange Book 2006 Humana Press 2006 SNP.microscopy.molecular biology.mutant.transcription [打印本頁] 作者: 拐杖 時間: 2025-3-21 19:56
書目名稱C. elegans影響因子(影響力)
書目名稱C. elegans影響因子(影響力)學(xué)科排名
書目名稱C. elegans網(wǎng)絡(luò)公開度
書目名稱C. elegans網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱C. elegans被引頻次
書目名稱C. elegans被引頻次學(xué)科排名
書目名稱C. elegans年度引用
書目名稱C. elegans年度引用學(xué)科排名
書目名稱C. elegans讀者反饋
書目名稱C. elegans讀者反饋學(xué)科排名
作者: 沖擊力 時間: 2025-3-21 21:32
https://doi.org/10.1007/978-1-349-15593-4erous experimental advantages for such studies. These advantages include a short life cycle, production of large numbers of offspring, easy and inexpensive laboratory culture, forward and reverse genetic tractability, and a relatively simple anatomy. This chapter will provide a brief overview of . biology.作者: 表皮 時間: 2025-3-22 02:14 作者: diathermy 時間: 2025-3-22 05:13 作者: CRP743 時間: 2025-3-22 10:53 作者: 討厭 時間: 2025-3-22 16:00
Creation of Transgenic Lines Using Microparticle Bombardment Methods,our different microparticle bombardment methods used to transform . with exogenous DNA. We include a discussion of the advantages and disadvantages of using micro-particle bombardment for transformation, list cotransformation markers that have been used successfully in microparticle bombardment experiments, and discuss transformation efficiency.作者: 討厭 時間: 2025-3-22 20:21
Intracellular Ca, Imaging in ,,rgeted to cells of interest with appropriate tissue-specific promoters. Here, we describe a specific protocol, suitable for monitoring neuronal activity, for rapid calcium imaging in . using the cameleon indicator. Notes are provided to assist with adapting this protocol for use with other indicators and slower time scales.作者: Addictive 時間: 2025-3-22 21:33
Stability and Introductory Dynamicsdience includes users wishing to query the database beyond the confines of the web interface or fetch data .. No knowledge of programming is necessary or assumed, although users with intermediate skills in the Perl scripting language will be able to utilize additional data-mining approaches.作者: 不發(fā)音 時間: 2025-3-23 03:48
Theory of Market Demand and Supply categorize the steps needed to do this as follows: (1) mutagenesis and DNA template preparation, (2) PCR detection of deletions, (3) sibling selection, and (4) deletion stabilization. These are discussed in detail in this chapter.作者: 外向者 時間: 2025-3-23 06:25 作者: 領(lǐng)帶 時間: 2025-3-23 12:33
C.-U. Herrmann,G. Klar,M. Kahlweitducting whole genome microarray experiments and for carrying out genome-wide reverse-genetic screens using a commercially available . bacterial RNAi feeding library. Both approaches are complimentary and can be used to rapidly gain genome-wide insights into the genes and gene networks controlling specific physiological processes.作者: 殺蟲劑 時間: 2025-3-23 14:01
J. Meril?,B. C. Sheldon,L. E. B. Kruuk of . crystal proteins toward .. The assays are quantitative growth measurement, brood size measurement, and determination of lethal concentration, as well as a qualitative health assay based on worm appearance. Although these assays are described for crystal proteins, the approaches are suited for the studies of other toxins with ..作者: DEMN 時間: 2025-3-23 18:59 作者: 大廳 時間: 2025-3-23 23:53 作者: Sciatica 時間: 2025-3-24 05:49
VLSI Interconnection by Bumpless TAB this technique is roughly one order of magnitude lower than chemical mutagens. However, the molecular identification of the mutated genes is extremely rapid. Therefore, before using .-mediated mutagenesis, one must evaluate the trade-off between time spent screening for mutants vs time spent mapping and rescuing a mutation.作者: Aggrandize 時間: 2025-3-24 07:25 作者: Irrigate 時間: 2025-3-24 11:58 作者: 聲音刺耳 時間: 2025-3-24 18:11
Multiscale Modeling of Gas Flows, and for the postembedding immunogold labeling of proteins contained within thin sections of embedded animals. These protocols enable high-resolution analysis of both morphological features and molecular domains within most tissues of ..作者: 弄皺 時間: 2025-3-24 21:43 作者: 空氣傳播 時間: 2025-3-25 00:08 作者: 幻影 時間: 2025-3-25 04:59
Electrophysiological Analysis of Neuronal and Muscle Function in ,, developed. This chapter will provide an overview of the technical requirements for patch-clamp electrophysiological analysis of . neurons and muscle cells, as well as provide some illustrative examples of insights gained from the pairing of electrophysiological techniques with molecular and genetic analysis.作者: cushion 時間: 2025-3-25 10:47
Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis,and proteomic applications, including those aiming to identify proteins based on affinity or biological activity. Germline mRNA expression profiles, RNAi feeding clones, and Gateway?-engineered ORFeome vectors are available to help validate experimental results.作者: Outspoken 時間: 2025-3-25 15:15 作者: 統(tǒng)治人類 時間: 2025-3-25 19:01
Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants,mic, and even behavioral relationships to individual cellular pHi can be inferred. In combination with dye loading of excised or cultured cells, this technique also provides a powerful means of contrasting these relationships to biophysical measurements of ion flux.作者: POINT 時間: 2025-3-25 22:38
WormBase,dience includes users wishing to query the database beyond the confines of the web interface or fetch data .. No knowledge of programming is necessary or assumed, although users with intermediate skills in the Perl scripting language will be able to utilize additional data-mining approaches.作者: 原始 時間: 2025-3-26 02:53
, Deletion Mutant Screening, categorize the steps needed to do this as follows: (1) mutagenesis and DNA template preparation, (2) PCR detection of deletions, (3) sibling selection, and (4) deletion stabilization. These are discussed in detail in this chapter.作者: placebo 時間: 2025-3-26 04:38 作者: 分開 時間: 2025-3-26 09:18 作者: Muffle 時間: 2025-3-26 13:53 作者: 小說 時間: 2025-3-26 18:08 作者: 搏斗 時間: 2025-3-26 23:56
In Vitro Culture of , Somatic Cells,ifferentiation within 24 h. Cultured embryo cells have been used recently to characterize ion channel function and regulation and to determine cell specific gene expression patterns. This chapter will provide a detailed description of the methods for isolating and culturing . embryo cells.作者: 盡責(zé) 時間: 2025-3-27 01:19 作者: 劇本 時間: 2025-3-27 07:48 作者: vibrant 時間: 2025-3-27 11:38 作者: erythema 時間: 2025-3-27 17:00 作者: 引起痛苦 時間: 2025-3-27 21:30
Theory of Exchange and General Equilibriumematode species. In this chapter, we describe the methods that we used to compare the . genome to that of .. We discuss how these methods could be developed to compare the . and . genomes to those of . n. sp. represented by strains PB2801 and CB5161, among others (.), and ., which are currently bein作者: 斜谷 時間: 2025-3-27 23:58
Stability and Introductory Dynamicsr to most . researchers, WormBase also offers powerful data-mining features for addressing questions of comparative genomics, genome structure, and evolution. In this chapter, we focus on data mining at WormBase through the use of flexible web interfaces, custom queries, and scripts. The intended au作者: ECG769 時間: 2025-3-28 04:05 作者: 不安 時間: 2025-3-28 09:49 作者: 羊欄 時間: 2025-3-28 10:44
https://doi.org/10.1007/978-94-009-0917-5ciated phenotype, making them ideal markers for mapping. SNP mapping has three steps. First, recombinant mutant animals are generated over a polymorphic strain (usually CB4856) using standard genetic techniques. Second, the genotype of these animals at SNP loci is determined using one of a variety o作者: 不能妥協(xié) 時間: 2025-3-28 18:20 作者: Cupping 時間: 2025-3-28 22:30 作者: indemnify 時間: 2025-3-29 00:29
J. Meril?,B. C. Sheldon,L. E. B. Kruuk. Quantitative parameters of . that can be assayed include growth, size, progeny production, behavior, and mortality. Qualitative parameters of toxicity, such as changes in appearance or movement, can also be determined. This chapter describes four assays we have used for analyzing the toxic effects作者: insular 時間: 2025-3-29 07:05
Matthias Adler,Sophie Waldschmidtgenetically encoded markers like the fluorescent proteins of the GFP family became the reporter of choice for gene expression studies and protein localization. The small size and transparency of the worm allows the visualization of in vivo dynamics, which increases the number of potential applicatio作者: Adulterate 時間: 2025-3-29 08:14 作者: 混雜人 時間: 2025-3-29 15:08
Prototype Applications of Gas Flowsl is that . is amenable to genetic manipulation. The complete genome sequence, advances in mass spectrometry, and RNA-mediated interference (RNAi) are now providing a technical infrastructure that complements biochemical and proteomic approaches. This chapter describes simple methods for sperm and o作者: 相容 時間: 2025-3-29 17:05
Multiscale Modeling of Gas Flows, an adult . hermaphrodite approaches its widest girth of approx 0.1 mm. In theory, an entire living adult animal can be physically immobilized instantly in amorphous ice by HPF, thus, providing a unique opportunity to examine cellular architecture with exquisite spatial preservation. The following 作者: Heretical 時間: 2025-3-29 23:24 作者: Vertical 時間: 2025-3-30 00:09
Numerical Methods for Continuum Simulationhes allow for precise quantitative definitions of behavioral phenotypes that have traditionally been subjectively described by individual observers. Automated systems also facilitate the analysis of behaviors that occur over long time scales or are difficult to detect by eye. Here we describe the de作者: 神圣將軍 時間: 2025-3-30 05:08 作者: 高腳酒杯 時間: 2025-3-30 10:39
Governing Equations and Slip Modelsted cells from larvae and adult worms is probably not technically feasible because of difficulties in removing the animal’s cuticle and dissociating cells. In contrast, large numbers of developing embryo cells can be isolated relatively easily. When placed in culture, embryo cells undergo terminal d作者: Perigee 時間: 2025-3-30 16:24
C. elegans978-1-59745-151-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: lymphedema 時間: 2025-3-30 19:06
Theory of Exchange and General Equilibriumematode species. In this chapter, we describe the methods that we used to compare the . genome to that of .. We discuss how these methods could be developed to compare the . and . genomes to those of . n. sp. represented by strains PB2801 and CB5161, among others (.), and ., which are currently being sequenced.作者: 招人嫉妒 時間: 2025-3-30 22:28
https://doi.org/10.1385/1597451517SNP; microscopy; molecular biology; mutant; transcription作者: STRIA 時間: 2025-3-31 01:35 作者: 激勵 時間: 2025-3-31 06:56 作者: geriatrician 時間: 2025-3-31 13:14 作者: 改變 時間: 2025-3-31 13:30
Delivery Methods for RNA Interference in ,,This chapter describes four methods for delivery of double-stranded RNA to . (injection, feeding, soaking, and in vivo delivery), and suggests schemes that should facilitate detection of specific gene silencing.作者: 炸壞 時間: 2025-3-31 20:22
Comparative Genomics in ,, and Other , Species,ematode species. In this chapter, we describe the methods that we used to compare the . genome to that of .. We discuss how these methods could be developed to compare the . and . genomes to those of . n. sp. represented by strains PB2801 and CB5161, among others (.), and ., which are currently being sequenced.作者: 軟膏 時間: 2025-3-31 23:36 作者: 推延 時間: 2025-4-1 02:04 作者: FORGO 時間: 2025-4-1 06:41 作者: 祖?zhèn)?nbsp; 時間: 2025-4-1 10:26
1064-3745 that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or “emergent” properties of complex, multicomponen978-1-61737-668-9978-1-59745-151-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Pander 時間: 2025-4-1 15:34 作者: Vertical 時間: 2025-4-1 19:23 作者: 蘑菇 時間: 2025-4-2 02:02
