標題: Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under excl [打印本頁] 作者: 一個希拉里 時間: 2025-3-21 17:40
書目名稱Bacterial Chromatin影響因子(影響力)
書目名稱Bacterial Chromatin影響因子(影響力)學科排名
書目名稱Bacterial Chromatin網絡公開度
書目名稱Bacterial Chromatin網絡公開度學科排名
書目名稱Bacterial Chromatin被引頻次
書目名稱Bacterial Chromatin被引頻次學科排名
書目名稱Bacterial Chromatin年度引用
書目名稱Bacterial Chromatin年度引用學科排名
書目名稱Bacterial Chromatin讀者反饋
書目名稱Bacterial Chromatin讀者反饋學科排名
作者: Fracture 時間: 2025-3-22 00:12 作者: 使閉塞 時間: 2025-3-22 03:49 作者: 傾聽 時間: 2025-3-22 05:26
ChIP-qPCR of FLAG-Tagged Proteins in Bacteriazation. Mapping the distribution of the DNA-bound proteins on the chromosome is essential for understanding their associated biological process. Chromatin immunoprecipitation (ChIP) involves the antibody-mediated enrichment of DNA fragments bound by a target protein and has become one of the most po作者: GRAVE 時間: 2025-3-22 12:45 作者: 壓迫 時間: 2025-3-22 13:52 作者: interrupt 時間: 2025-3-22 19:37 作者: colostrum 時間: 2025-3-22 21:15 作者: dithiolethione 時間: 2025-3-23 02:35 作者: BLOT 時間: 2025-3-23 05:46 作者: Factual 時間: 2025-3-23 12:06 作者: 學術討論會 時間: 2025-3-23 16:13
Genetic Engineering of , Using Competence-Induced Homologous Recombination Techniquesa . cell can initiate one out of various distinct differentiation processes to cope with changing environmental conditions. One of these differentiation processes is natural competence that allows cells to adsorb exogenous DNA and subsequently incorporate it into its chromosome by homologous recombi作者: artless 時間: 2025-3-23 20:11
Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organizationimple cell manipulation procedure that enables stepwise dissection of the nucleoid. The procedure includes (i) on-substrate lysis of cells and (ii) enzyme treatment, followed by atomic force microscopy. This type of dissection analysis permits analysis of nucleoid structure ranging from the fundamen作者: gruelling 時間: 2025-3-24 00:38
Atomic Force Microscopy Characterization of Reconstituted Protein-DNA Complexes straightforward method to characterize the binding behavior of different chromatin architectural proteins and to analyze the increasingly complex structural units assembled on the DNA. The protocol describes the preparation, AFM imaging, and structural analysis of chromatin that is reconstituted in作者: MEN 時間: 2025-3-24 05:19
Approaches for Determining DNA Persistence Length Using Atomic Force Microscopynical properties of DNA polymers depend on electrostatics and the strength of DNA base stacking by studying double-stranded DNA molecules incorporating several different neutral and charged base modifications. Here we describe ten complementary approaches for determining DNA persistence length by AF作者: lymphoma 時間: 2025-3-24 07:16 作者: 發(fā)微光 時間: 2025-3-24 12:21 作者: Brittle 時間: 2025-3-24 15:12
Book 2024Latest edition...?..Authoritative and cutting-edge,?.Bacterial Chromatin: Methods and Protocols, Second Edition .aims to be a?useful up-to-date reference work for researchers currently in the field and to those entering the field..作者: 引水渠 時間: 2025-3-24 22:27
Chavaunne T. Thorpe,Hazel R. C. Screenre (3C) methods revolutionized our ability to explore the hierarchical structure and the dynamics of bacterial genomes. Here, we review the major advances in the field of bacterial chromosome structure using 3C technology over the past decade.作者: 運動的我 時間: 2025-3-25 02:32 作者: 貿易 時間: 2025-3-25 04:48 作者: 沒收 時間: 2025-3-25 08:59 作者: Basilar-Artery 時間: 2025-3-25 13:37 作者: VEIL 時間: 2025-3-25 17:24 作者: Cognizance 時間: 2025-3-25 21:15
GeF-seq: A Simple Procedure for Base-Pair Resolution ChIP-seqir resolution. GeF-seq detects binding sites of DBPs as sharp peaks and thus makes it possible to identify the recognition sequence in each “binding peak” more easily and accurately compared to the common ChIP-seq.作者: 不如屎殼郎 時間: 2025-3-26 01:32
ChIP-qPCR of FLAG-Tagged Proteins in Bacteriaibe ChIP-qPCR. We provide a step-by-step protocol for the preparation of a ChIP library of a 3× FLAG-tagged protein in bacteria, describe how downstream qPCR experiments can be performed with the appropriate controls, and explain how the data is analyzed. This chapter provides reliable technical guidance for ChIP-qPCR studies in bacteria.作者: 允許 時間: 2025-3-26 08:02 作者: Rustproof 時間: 2025-3-26 09:04 作者: BROOK 時間: 2025-3-26 12:38 作者: Mingle 時間: 2025-3-26 18:45 作者: 間接 時間: 2025-3-27 00:29
Getting on the Path to Engineering Biologyg several different neutral and charged base modifications. Here we describe ten complementary approaches for determining DNA persistence length by AFM imaging. The combination of different approaches provides increased confidence and statistical reliability over existing methods utilizing only a single approach.作者: 大包裹 時間: 2025-3-27 01:17 作者: 下級 時間: 2025-3-27 07:20 作者: enmesh 時間: 2025-3-27 10:03 作者: 精美食品 時間: 2025-3-27 15:13
Prashant Chauhan,Arup Sarkar,Bhaskar Sahaing. Here we describe a detailed protocol of the gSELEX screening system, which uses purified regulatory proteins and fragments of genomic DNA from .. Moreover, we describe methods and examples of results using cell-free synthetic proteins.作者: Palpable 時間: 2025-3-27 19:30 作者: ALIAS 時間: 2025-3-28 01:41
O. Wieland,E. Helmreich,H. Holzercontact. The procedure described here will suffice to provide a comprehensive map of transposition frequencies between tens of thousands of loci in a bacterial genome, with the resolution limited by the diversity of the insertion site library used and the sequencing depth applied.作者: 歸功于 時間: 2025-3-28 05:54 作者: 上腭 時間: 2025-3-28 08:06
Metabolic Interconversion of Enzymes 1980nation. Due to competence development, the genome of . can be easily manipulated, and this has contributed to . being a model system. In this chapter, we describe some of the most common genetic tools that can be used in combination with natural competence to tailor the genome of ..作者: 神秘 時間: 2025-3-28 11:14 作者: Coronary-Spasm 時間: 2025-3-28 14:38 作者: UTTER 時間: 2025-3-28 22:16 作者: Corroborate 時間: 2025-3-29 01:54
High-Resolution Characterization of DNA/Protein Complexes in Living Bacteriavage (ChEC), coupled with ligation-mediated polymerase chain reaction (LM-PCR) and Southern blot analysis. As an example, we apply these in vivo protein-mapping methods to . to show direct binding of architectural proteins in the Lac repressor-mediated DNA repression loop.作者: 迅速飛過 時間: 2025-3-29 06:09
High-Throughput Mapping of Chromosomal Conformations in , Under Physiological Conditions Using Massicontact. The procedure described here will suffice to provide a comprehensive map of transposition frequencies between tens of thousands of loci in a bacterial genome, with the resolution limited by the diversity of the insertion site library used and the sequencing depth applied.作者: Asymptomatic 時間: 2025-3-29 08:16 作者: Prognosis 時間: 2025-3-29 15:01 作者: GREEN 時間: 2025-3-29 16:08 作者: Factorable 時間: 2025-3-29 23:25 作者: 有惡意 時間: 2025-3-30 01:40
Visualization, Quantification, and Statistical Evaluation of Dynamics for DNA-Binding Proteins in Ban dynamics in mutant cells or cells exposed to changes in environmental conditions..In this chapter, we describe the preparation of . cells, the recording of movies of those cells expressing a monomeric variant of a yellow fluorescent protein (mNeonGreen) fused to a protein of choice, and the subseq作者: ZEST 時間: 2025-3-30 06:55 作者: Complement 時間: 2025-3-30 08:37
1064-3745 g and avoiding known pitfalls...?..Authoritative and cutting-edge,?.Bacterial Chromatin: Methods and Protocols, Second Edition .aims to be a?useful up-to-date reference work for researchers currently in the field and to those entering the field..978-1-0716-3932-0978-1-0716-3930-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 智力高 時間: 2025-3-30 12:42 作者: 中止 時間: 2025-3-30 20:22
Learning Materials in Bioscienceses on the wide range of ways in which these experiments are set up and the diverse types of information they reveal. These aspects are illustrated with four very different systems: the sequence-dependent DNA compaction by architectural protein HMfB, the sequential binding of core histone complexes t作者: 生意行為 時間: 2025-3-30 21:21
https://doi.org/10.1007/978-1-0716-3930-63D genome organization; Hi-C derivatives; SELEX; 3D-seq; RNA-seq作者: 演講 時間: 2025-3-31 01:46 作者: 咒語 時間: 2025-3-31 05:19
Remus T. DameIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 和諧 時間: 2025-3-31 12:22 作者: Isometric 時間: 2025-3-31 13:23
Chavaunne T. Thorpe,Hazel R. C. Screeneristics. Among the many complexities of genetic material organization, the folding and arrangement of DNA into chromosomes play a critical role in regulating gene expression, replication, and other essential cellular processes. Bacteria, despite their apparently simple cellular structure, exhibit a作者: 蛛絲 時間: 2025-3-31 20:13 作者: Anthropoid 時間: 2025-4-1 00:13
Inês Mesquita,Fernando Rodriguesomosome segregation, cell division, and nucleoid structure in bacterial cells. Therefore, determination of the binding sequences of DBPs is important not only to study DBP recognition mechanisms but also to understand the fundamentals of cell homeostasis. While ChIP-seq analysis appears to be an eff作者: 該得 時間: 2025-4-1 05:04 作者: Ccu106 時間: 2025-4-1 06:59
Prashant Chauhan,Arup Sarkar,Bhaskar Sahath one of seven species of the sigma subunit and a total of approximately 300 species of transcription factor (TFs). For comprehensive identification of the regulatory targets of these two groups of regulatory proteins on the genome, we developed an in vitro approach, “Genomic SELEX” (gSELEX) screen作者: ironic 時間: 2025-4-1 11:23 作者: lavish 時間: 2025-4-1 16:40
