標題: Titlebook: Breast Cancer; Methods and Protocol Jian Cao Book 2016 Springer Science+Business Media New York 2016 diagnosing breast cancer.clinical prac [打印本頁] 作者: deep-sleep 時間: 2025-3-21 19:10
書目名稱Breast Cancer影響因子(影響力)
書目名稱Breast Cancer影響因子(影響力)學科排名
書目名稱Breast Cancer網(wǎng)絡公開度
書目名稱Breast Cancer網(wǎng)絡公開度學科排名
書目名稱Breast Cancer被引頻次
書目名稱Breast Cancer被引頻次學科排名
書目名稱Breast Cancer年度引用
書目名稱Breast Cancer年度引用學科排名
書目名稱Breast Cancer讀者反饋
書目名稱Breast Cancer讀者反饋學科排名
作者: biopsy 時間: 2025-3-21 23:08 作者: 過份好問 時間: 2025-3-22 01:32
Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.作者: PARA 時間: 2025-3-22 08:28 作者: 催眠 時間: 2025-3-22 12:34
Clinical Applications for Immunohistochemistry of Breast Lesionsies to the best course of clinical action. In this chapter, we discuss the use of immunohistochemistry testing for some of the key molecular markers involved in breast pathology that are crucial for classifying breast cancers and the guidelines for the interpretation of testing results that assist in clinical management.作者: maintenance 時間: 2025-3-22 16:54 作者: 全神貫注于 時間: 2025-3-22 18:40 作者: MOTIF 時間: 2025-3-22 22:22
Breast Cancer Stem Cell Isolationhus, the technique to identify and purify the cancer stem cell is the key in any cancer stem cell research. In this protocol, we provide the basic technology of identification and purification of breast cancer stem cells as well as further functional assays to help the researchers achieve their research goals.作者: Perennial長期的 時間: 2025-3-23 03:34
Cellular Apoptosis Assay of Breast Cancerchondrial transmembrane potential, an early event of mitochondrial involvement, is also included. Of note, since apoptotic features may not appear to the same extent depending on the context of cell types and the death-inducing insults, a common practice is to use more than one method to assess apoptosis, qualitatively and quantitatively.作者: LINES 時間: 2025-3-23 07:37
Assessment of Matrix Metalloproteinases by Gelatin Zymographyhe enzymes are refolded in order for proteolysis of the incorporated substrate to occur. This assay yields valuable information about MMP isoforms or changes in activation and can be used to analyze the role of MMPs in normal versus pathological conditions.作者: 叢林 時間: 2025-3-23 10:19 作者: Airtight 時間: 2025-3-23 14:46 作者: 揮舞 時間: 2025-3-23 21:32
https://doi.org/10.1007/978-3-642-41769-6stochemical markers can be used as a surrogate for genomic profiling and thus to identify basal-like breast cancers. We describe the panels of immunohistochemical markers that can be applied and how to interpret these markers herein.作者: Cuisine 時間: 2025-3-23 23:32
M. D. Lechner,E. H. Nordmeier,K. Gehrke biological, pathological, and clinical roles of genes in health and disease. This chapter describes a real-time PCR approach to detect and quantify mRNA expression levels, which can be used for both laboratorial and clinical studies in breast cancer research.作者: 人類 時間: 2025-3-24 03:37 作者: 畢業(yè)典禮 時間: 2025-3-24 06:31
Harry Melville K.C.B., Ph.D., D.Sc., F.R.S.chondrial transmembrane potential, an early event of mitochondrial involvement, is also included. Of note, since apoptotic features may not appear to the same extent depending on the context of cell types and the death-inducing insults, a common practice is to use more than one method to assess apoptosis, qualitatively and quantitatively.作者: Carbon-Monoxide 時間: 2025-3-24 12:32
https://doi.org/10.1007/978-3-662-55956-7he enzymes are refolded in order for proteolysis of the incorporated substrate to occur. This assay yields valuable information about MMP isoforms or changes in activation and can be used to analyze the role of MMPs in normal versus pathological conditions.作者: 孤僻 時間: 2025-3-24 14:53
M. D. Lechner,E. H. Nordmeier,K. Gehrke not go into any chemical change during this reaction. Next, bisulfite treated DNA is used in the regular PCR reaction to get a single band PCR amplicon which will be used as a template for the pyrosequencing. Pyrosequencing is a powerful method to make a quantitative methylation analysis for each specific CG pair.作者: 提名的名單 時間: 2025-3-24 19:30 作者: fiscal 時間: 2025-3-25 01:11
Historie und Begriffsdefinition,ly for basic cancer biology but especially for drug development (Friedl and Gilmour, Nat Rev Mol Cell Biol 10: 445–457, 2009). Researchers continue to develop new techniques for measuring cell migration in vitro. This chapter will discuss two techniques commonly used to study cell migration: wound healing and Boyden chamber migration assays.作者: Osteoarthritis 時間: 2025-3-25 06:00
Determination of Breast Cancer Cell Migratory Abilityly for basic cancer biology but especially for drug development (Friedl and Gilmour, Nat Rev Mol Cell Biol 10: 445–457, 2009). Researchers continue to develop new techniques for measuring cell migration in vitro. This chapter will discuss two techniques commonly used to study cell migration: wound healing and Boyden chamber migration assays.作者: DEI 時間: 2025-3-25 08:05 作者: 預防注射 時間: 2025-3-25 13:25
https://doi.org/10.1007/978-1-4939-3444-7diagnosing breast cancer; clinical practice; molecular markers of breast cancer; genetic alterations; in作者: 捏造 時間: 2025-3-25 17:26 作者: paltry 時間: 2025-3-25 20:22
Breast Cancer978-1-4939-3444-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AV-node 時間: 2025-3-26 01:34 作者: 古老 時間: 2025-3-26 05:34
Der Lebenszyklus von Makroplastik,f effective assays for monitoring cancer cell migration. Here we describe a novel method to microscopically monitor cell migration in a quantitative fashion. This assay can be used to study genes involved in cancer cell migration, as well as screening anticancer drugs that target this cellular process.作者: 利用 時間: 2025-3-26 10:17 作者: 治愈 時間: 2025-3-26 13:33 作者: 葡萄糖 時間: 2025-3-26 20:28 作者: 裂口 時間: 2025-3-26 21:05 作者: 臆斷 時間: 2025-3-27 02:13
Jian CaoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: meretricious 時間: 2025-3-27 07:23
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/190540.jpg作者: 一個攪動不安 時間: 2025-3-27 11:55 作者: LAITY 時間: 2025-3-27 17:36 作者: 增強 時間: 2025-3-27 17:46
https://doi.org/10.1007/978-3-642-41769-6strictly defined as invasive carcinomas lacking expression of estrogen receptor, progesterone receptor, and HER2, some use the terms triple-negative and basal-like cancer synonymously. It should be noted that these are not entirely equivalent. Nevertheless, it has been shown that a panel of immunohi作者: Fulminate 時間: 2025-3-27 22:08
,Qualitative Analyse von Makromolekülen, for anti-HER2 antibody therapy and should be performed in accordance with current ASCO-CAP guidelines (Hammond et al., J Clin Oncol 29(15):e458, 2011; Wolff et al., J Clin Oncol 31(31):3997–4013, 2013). Here we describe a HER2 FISH methodology to evaluate HER2 gene status in FFPE breast tumor speci作者: 緩和 時間: 2025-3-28 05:00 作者: Dawdle 時間: 2025-3-28 08:57
M. D. Lechner,E. H. Nordmeier,K. Gehrkede of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA s作者: 完整 時間: 2025-3-28 13:42
M. D. Lechner,E. H. Nordmeier,K. Gehrkesamples, it is very difficult to get methylation profile of only tumor cells. Laser capture Microdissection (LCM) is giving us a chance to isolate the DNA only from the tumor cells without any stroma cell’s DNA contamination. Once we capture the breast tumor cells, we can isolate the genomic DNA whi作者: Soliloquy 時間: 2025-3-28 14:49
https://doi.org/10.1007/978-3-0348-5094-0sitivity testing of short-term cultures of CTCs, single cell sorting of CTCs by fluorescence activated cell sorting (FACS), animal injection tumor and/or metastasis formation studies, next generation sequencing (NGS), gene expression profiling, gene copy number determination, and epigenomic analyses作者: Ceremony 時間: 2025-3-28 20:55 作者: 皺痕 時間: 2025-3-29 01:22
Harry Melville K.C.B., Ph.D., D.Sc., F.R.S.y introduce methodologies to assay caspases dependent biochemical and morphological changes in vitro breast cancer cell lines and in vivo breast cancer tissues. In addition, mitochondrial involvement is crucial to distinguish two different apoptotic pathways. Methodology to assay dissipation of mito作者: NATAL 時間: 2025-3-29 07:01
https://doi.org/10.1007/978-3-662-55956-7to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression. In this approach, enzymes are resolved electrophoretically on a sodium dodecyl sulfate-polyacrylamide gel copolymerized with the substrate for the MMP of interest. Post electrophoresis, t作者: epidermis 時間: 2025-3-29 09:00
https://doi.org/10.1007/978-3-662-55956-7M). While MMPs are involved in many normal biological processes, unregulated MMP activity has been linked to many detrimental diseases, including cancer, neurodegenerative diseases, stroke, and cardiovascular disease. Developed as tools to investigate MMP function and as potential new therapeutics, 作者: 闖入 時間: 2025-3-29 15:22 作者: 落葉劑 時間: 2025-3-29 18:44
Der Lebenszyklus von Makroplastik,f effective assays for monitoring cancer cell migration. Here we describe a novel method to microscopically monitor cell migration in a quantitative fashion. This assay can be used to study genes involved in cancer cell migration, as well as screening anticancer drugs that target this cellular proce作者: Chromatic 時間: 2025-3-29 22:31 作者: BLAND 時間: 2025-3-30 03:19 作者: 預知 時間: 2025-3-30 05:07 作者: 混亂生活 時間: 2025-3-30 08:39 作者: VERT 時間: 2025-3-30 12:32
Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)s can be quantified by this approach. It is well known that changes in mRNA expression in disease, and correlation of mRNA expression profiles with clinical parameters, serve as clinically relevant biomarkers. Hence, accurate determination of the mRNA levels is critically important in describing the作者: 修剪過的樹籬 時間: 2025-3-30 16:33
Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancerde of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA s作者: 送秋波 時間: 2025-3-30 22:14
Pyrosequencing Analysis for Breast Cancer DNA Methylomesamples, it is very difficult to get methylation profile of only tumor cells. Laser capture Microdissection (LCM) is giving us a chance to isolate the DNA only from the tumor cells without any stroma cell’s DNA contamination. Once we capture the breast tumor cells, we can isolate the genomic DNA whi作者: 公共汽車 時間: 2025-3-31 02:20
Vita-Assay? Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cesitivity testing of short-term cultures of CTCs, single cell sorting of CTCs by fluorescence activated cell sorting (FACS), animal injection tumor and/or metastasis formation studies, next generation sequencing (NGS), gene expression profiling, gene copy number determination, and epigenomic analyses作者: peptic-ulcer 時間: 2025-3-31 06:08 作者: MOCK 時間: 2025-3-31 11:18
Cellular Apoptosis Assay of Breast Cancery introduce methodologies to assay caspases dependent biochemical and morphological changes in vitro breast cancer cell lines and in vivo breast cancer tissues. In addition, mitochondrial involvement is crucial to distinguish two different apoptotic pathways. Methodology to assay dissipation of mito作者: Enthralling 時間: 2025-3-31 13:49
Assessment of Matrix Metalloproteinases by Gelatin Zymographyto breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression. In this approach, enzymes are resolved electrophoretically on a sodium dodecyl sulfate-polyacrylamide gel copolymerized with the substrate for the MMP of interest. Post electrophoresis, t作者: 蒸發(fā) 時間: 2025-3-31 21:19
Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate AssayM). While MMPs are involved in many normal biological processes, unregulated MMP activity has been linked to many detrimental diseases, including cancer, neurodegenerative diseases, stroke, and cardiovascular disease. Developed as tools to investigate MMP function and as potential new therapeutics,
