作者: Consequence 時(shí)間: 2025-3-22 00:09 作者: certain 時(shí)間: 2025-3-22 02:02 作者: 斜 時(shí)間: 2025-3-22 06:15
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/190439.jpg作者: 修飾 時(shí)間: 2025-3-22 12:19
Brassinosteroids978-1-4939-6813-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Juvenile 時(shí)間: 2025-3-22 13:23
Joachim St?hr,Hans Christoph Siegmannocesses, including root and shoot growth, vascular differentiation, fertility, and seed germination. A characteristic feature of all plant hormones, including BRs, is that their concentration is extremely low in plant tissues and, therefore, the methods dealing with their determination belong to ult作者: 莎草 時(shí)間: 2025-3-22 18:44
Polarized Electrons and Magnetismluorescent analogs. However, most biologically active BRs are not suitable for direct chemical conjugation techniques because their derivatization typically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify 作者: Antecedent 時(shí)間: 2025-3-23 00:06 作者: 臆斷 時(shí)間: 2025-3-23 04:25 作者: 鍍金 時(shí)間: 2025-3-23 09:20 作者: Chagrin 時(shí)間: 2025-3-23 11:25 作者: Occlusion 時(shí)間: 2025-3-23 17:45
Polarized Electrons and MagnetismSUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-through作者: PANG 時(shí)間: 2025-3-23 21:49 作者: ROOF 時(shí)間: 2025-3-23 23:15
Joachim St?hr,Hans Christoph Siegmann as to make predictions and select the most efficient follow-up experiments. Here we outline a method to model systems of a few proteins that interact transcriptionally and/or posttranscriptionally, by representing the system as Ordinary Differential Equations and to study the model dynamics and sta作者: syncope 時(shí)間: 2025-3-24 06:21
Joachim St?hr,Hans Christoph Siegmann of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endom作者: expunge 時(shí)間: 2025-3-24 08:34 作者: 歹徒 時(shí)間: 2025-3-24 13:37 作者: sed-rate 時(shí)間: 2025-3-24 16:33 作者: 毗鄰 時(shí)間: 2025-3-24 21:20
Polarized Electromagnetic WavesD INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants作者: 牢騷 時(shí)間: 2025-3-24 23:54
Polarized Electrons and Magnetism plant model system . has yielded important advances in the molecular and cellular understanding of root growth and development. Several studies have uncovered how the hormones brassinosteroids (BRs) control cell cycle and differentiation programs through different cell-specific signaling pathways t作者: 蛛絲 時(shí)間: 2025-3-25 03:31 作者: 反復(fù)無(wú)常 時(shí)間: 2025-3-25 11:31
Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues,ocesses, including root and shoot growth, vascular differentiation, fertility, and seed germination. A characteristic feature of all plant hormones, including BRs, is that their concentration is extremely low in plant tissues and, therefore, the methods dealing with their determination belong to ult作者: 慷慨不好 時(shí)間: 2025-3-25 14:59 作者: 左右連貫 時(shí)間: 2025-3-25 17:30 作者: grudging 時(shí)間: 2025-3-25 22:34
Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability be a method to observe changes in protein stability under different light conditions. In brief, . seedlings were maintained under various light regimes from continuous light to total darkness or transitions from light to dark, whereafter total protein was extracted from plants. Proteins were measure作者: 過(guò)多 時(shí)間: 2025-3-26 02:50
Approaches to Study Light Effects on Brassinosteroid Sensitivity, responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In . seedlings, hypocotyl length is strongly affected by light condi作者: 起皺紋 時(shí)間: 2025-3-26 07:03 作者: 蒸發(fā) 時(shí)間: 2025-3-26 09:08
Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-ThSUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-through作者: canvass 時(shí)間: 2025-3-26 12:40
Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Assone expression, has therefore been the focus of many studies. These analyses have been primarily conducted on total extracts derived from a mixture of tissues and cell types, consequentially limiting delineation of precise models. In this chapter, methods for tissue-specific functional genomics are o作者: projectile 時(shí)間: 2025-3-26 18:24 作者: 抒情短詩(shī) 時(shí)間: 2025-3-26 23:19
Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells, of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endom作者: Concerto 時(shí)間: 2025-3-27 01:40 作者: itinerary 時(shí)間: 2025-3-27 07:31
Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass SpectrometrSpectrometry/Mass Spectrometry (LCMS/MS) has proven to be a reliable method to qualitatively characterize membrane-bound receptor complexes from plants. Success depends on a range of parameters, such as abundance and stability of the complex and functionality of the tagged receptors, efficiency of t作者: Sputum 時(shí)間: 2025-3-27 13:11
Simplified Enrichment of Plasma Membrane Proteins from , Seedlings Using Differential Centrifugatios, including perception of extracellular signals to coordinate cellular changes. Analyses of PM proteins, however, are often limited by the relatively low abundance of these proteins in the total cellular protein pool. Techniques traditionally used for enrichment of PM proteins are time consuming, t作者: 道學(xué)氣 時(shí)間: 2025-3-27 17:38
Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by ImmunoprD INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants作者: 縮減了 時(shí)間: 2025-3-27 20:55
The Primary Root of , , (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops, plant model system . has yielded important advances in the molecular and cellular understanding of root growth and development. Several studies have uncovered how the hormones brassinosteroids (BRs) control cell cycle and differentiation programs through different cell-specific signaling pathways t作者: 大量殺死 時(shí)間: 2025-3-27 23:18 作者: 連詞 時(shí)間: 2025-3-28 02:12 作者: 傾聽(tīng) 時(shí)間: 2025-3-28 07:41 作者: 把…比做 時(shí)間: 2025-3-28 13:52
Springer Series in Solid-State Sciences interest, allowing tissue-specific immunopurification of the polysome complexes. The methods presented assess establishment and selection of suitable transgenic lines. A protocol for hormonal treatment of the . root as a case study, TRAP and linear amplification of the purified polysome-associated 作者: 難理解 時(shí)間: 2025-3-28 18:19
Polarized Electrons and Magnetismass transmembrane domain. The latest updated laboratory protocol is presented as well as examples of data analysis and typical results obtained. Potential drawbacks of the procedure employed for plant membrane proteins will be pointed out.作者: 發(fā)怨言 時(shí)間: 2025-3-28 22:30 作者: 厚顏 時(shí)間: 2025-3-29 00:48 作者: Ingenuity 時(shí)間: 2025-3-29 03:07
Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues,acts. The protocol is designed for sensitive liquid chromatography-tandem mass spectrometry-based method for simultaneous detection of 22 naturally occurring BRs, including their biosynthetic precursors and most of their biologically active metabolites, without need for derivatization.作者: 詩(shī)集 時(shí)間: 2025-3-29 08:12
Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Asso interest, allowing tissue-specific immunopurification of the polysome complexes. The methods presented assess establishment and selection of suitable transgenic lines. A protocol for hormonal treatment of the . root as a case study, TRAP and linear amplification of the purified polysome-associated 作者: 發(fā)誓放棄 時(shí)間: 2025-3-29 14:01
Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometrass transmembrane domain. The latest updated laboratory protocol is presented as well as examples of data analysis and typical results obtained. Potential drawbacks of the procedure employed for plant membrane proteins will be pointed out.作者: 引起 時(shí)間: 2025-3-29 16:45
The Primary Root of , , (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops,y of the BRASSINOSTEROID INSENSITIVE1-like receptor family in . and its orthologous genes in sorghum. Overall, we support the use of sorghum as a suitable crop model species for the study of BR signaling in root growth and development. The methods presented enable any laboratory worldwide to use sor作者: Confound 時(shí)間: 2025-3-29 23:27 作者: Fulminate 時(shí)間: 2025-3-30 02:24
Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Thput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.作者: Adherent 時(shí)間: 2025-3-30 05:54
1064-3745 results.Contains key implementation advice from the expertsThis detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques作者: 焦慮 時(shí)間: 2025-3-30 11:28
Polarized Electrons and Magnetismically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify an Alexa Fluor 647-castasterone (AFCS) from commercially available materials. The reported strategy is also amenable to the introduction of various other amine-based labeling groups.作者: 注射器 時(shí)間: 2025-3-30 12:52
Springer Series in Solid-State Sciencescribe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (.) seedlings.作者: Contracture 時(shí)間: 2025-3-30 16:43 作者: 載貨清單 時(shí)間: 2025-3-30 22:47 作者: macrophage 時(shí)間: 2025-3-31 00:57 作者: fidelity 時(shí)間: 2025-3-31 07:49
Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprifferent posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein–protein interactions.作者: TRAWL 時(shí)間: 2025-3-31 10:26 作者: granite 時(shí)間: 2025-3-31 15:49 作者: 匯總 時(shí)間: 2025-3-31 19:58
Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability responding antibodies for the visualization of protein bands. The protocol described has been successfully applied in wild-type, different transgenic, and mutant background plants to study how light alone or in combination with other factors influences protein stability.作者: 寬敞 時(shí)間: 2025-3-31 22:11
