標(biāo)題: Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na [打印本頁(yè)] 作者: lutein 時(shí)間: 2025-3-21 18:40
書目名稱Brain Development影響因子(影響力)
書目名稱Brain Development影響因子(影響力)學(xué)科排名
書目名稱Brain Development網(wǎng)絡(luò)公開度
書目名稱Brain Development網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Brain Development被引頻次
書目名稱Brain Development被引頻次學(xué)科排名
書目名稱Brain Development年度引用
書目名稱Brain Development年度引用學(xué)科排名
書目名稱Brain Development讀者反饋
書目名稱Brain Development讀者反饋學(xué)科排名
作者: galley 時(shí)間: 2025-3-21 23:07 作者: 廚師 時(shí)間: 2025-3-22 03:54
Computational Methods in Applied Sciencesthe fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene?knockout.作者: FUSC 時(shí)間: 2025-3-22 08:13 作者: 毛細(xì)血管 時(shí)間: 2025-3-22 09:57
CRISPR/Cas9 Genome Editing to Study Nervous System Development in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene?knockout.作者: indoctrinate 時(shí)間: 2025-3-22 14:38
A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hyeps of cell fate specification to be followed at a single cell resolution. This protocol describes preparation of . embryos, classical in situ hybridization protocol coupled with nuclear staining, and a guide to identify gene expression in specific precursors of the developing brain at neural plate stages of development.作者: 態(tài)度暖昧 時(shí)間: 2025-3-22 18:24 作者: 多樣 時(shí)間: 2025-3-23 00:34 作者: 凝視 時(shí)間: 2025-3-23 02:25
Vladimir Leble,George N. Barakosrotocols for wholemount and flat preparations of . embryos, which allow a more detailed analysis of gene expression patterns in relation to the cellular context of the early brain (and facilitate the identification of individual brain neuroblasts) using conventional light microscopy.作者: MEET 時(shí)間: 2025-3-23 07:47
Zhengshun Cheng,Torgeir Moan,Zhen Gaoin multiple colors. This is followed by a detailed protocol as to how to prepare samples for imaging. Finally, we provide specifications to facilitate multichannel image acquisition using confocal microscopy.作者: 脫毛 時(shí)間: 2025-3-23 11:17 作者: 假裝是你 時(shí)間: 2025-3-23 15:10 作者: Intellectual 時(shí)間: 2025-3-23 21:04
The Search for Life: Past and Present,stages—usually 70–300?μm in size—as well as for vibratome and cryostat sections of complex brains. Although our procedures have been optimized for marine molluscs, they may easily be adapted to other (marine) organisms by the creative neurobiologist.作者: Agility 時(shí)間: 2025-3-23 22:30 作者: lanugo 時(shí)間: 2025-3-24 02:27 作者: NOVA 時(shí)間: 2025-3-24 09:14 作者: 熱烈的歡迎 時(shí)間: 2025-3-24 11:38
Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain, as new model system. The embryonic development of marbled crayfish is well characterized and these animals can be easily cultivated in the lab. This chapter describes protocols for immunolocalization of neuroactive substances in the developing crayfish brain.作者: 樂(lè)意 時(shí)間: 2025-3-24 15:43
Methods in Brain Development of Molluscsstages—usually 70–300?μm in size—as well as for vibratome and cryostat sections of complex brains. Although our procedures have been optimized for marine molluscs, they may easily be adapted to other (marine) organisms by the creative neurobiologist.作者: 鞭子 時(shí)間: 2025-3-24 20:54 作者: Monocle 時(shí)間: 2025-3-25 01:45 作者: RAG 時(shí)間: 2025-3-25 06:15 作者: Figate 時(shí)間: 2025-3-25 08:00
Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in abeling can be combined with the analysis of gene expression by whole-mount in situ hybridization. This co-immunodetection procedure is well adapted to visualize and quantify the dynamics of de novo neurogenesis. Upon continuous BrdU labeling, the repeated measurements of BrdU-labeling indexes in sp作者: guzzle 時(shí)間: 2025-3-25 13:45 作者: 浮夸 時(shí)間: 2025-3-25 16:11 作者: 絕種 時(shí)間: 2025-3-25 21:39
Immunostaining and In Situ Hybridization of the Developing Acoel Nervous Systemin some of these protocols in detail, with the aim that should prove useful in our much-needed understanding of the origins of bilaterian animals. An anatomical sketch is provided at the beginning as a necessary guide for those not familiar with the Acoela.作者: Negotiate 時(shí)間: 2025-3-26 01:48 作者: groggy 時(shí)間: 2025-3-26 07:50
Analysis of Complete Neuroblast Cell Lineages in the , Embryonic Brain via DiI Labelingdual cells within a labeled NB lineage. These protocols make it feasible to uncover precise lineage relationships between a brain NB and its daughter cells, and to assign gene expression to individual clonal cells. Such lineage-based information is a critical key for understanding the cellular and m作者: 羅盤 時(shí)間: 2025-3-26 10:39
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Emre complementary to mature mRNAs often produce diffuse signals. We demonstrate that RNA in situ probes complementary to intronic gene sequences facilitate single cell resolution because the fluorescent signal is restricted to the nucleus. We believe our protocols can be adapted easily to suit the an作者: 歪曲道理 時(shí)間: 2025-3-26 14:19 作者: 勛章 時(shí)間: 2025-3-26 18:37 作者: Mundane 時(shí)間: 2025-3-26 21:37
MAP and TOP Application Case Studies,abeling can be combined with the analysis of gene expression by whole-mount in situ hybridization. This co-immunodetection procedure is well adapted to visualize and quantify the dynamics of de novo neurogenesis. Upon continuous BrdU labeling, the repeated measurements of BrdU-labeling indexes in sp作者: chemoprevention 時(shí)間: 2025-3-27 03:17 作者: Defiance 時(shí)間: 2025-3-27 08:02
Curl-Conformin Basis Functions, cnidarian model organism for studying the evolution of developmental processes. We also provide an overview of existing transgenic . lines that have been used to study conserved and derived aspects of nervous system development.作者: 煩人 時(shí)間: 2025-3-27 09:37
Vladimir Leble,George N. Barakosin some of these protocols in detail, with the aim that should prove useful in our much-needed understanding of the origins of bilaterian animals. An anatomical sketch is provided at the beginning as a necessary guide for those not familiar with the Acoela.作者: Relinquish 時(shí)間: 2025-3-27 16:37
Duje Veic,Marek Kraskowski,Tomasz Bugalskions. Thus, antibody probes that recognize defined tissues, cells, or subcellular structures like axons or synaptic terminals can be used as markers to identify and analyze phenotypes in embryos and larvae. Several antibodies, combined with different labels can be used concurrently to examine protein作者: 蠟燭 時(shí)間: 2025-3-27 18:48
Emilio Di Lorenzo,Simone Manzatodual cells within a labeled NB lineage. These protocols make it feasible to uncover precise lineage relationships between a brain NB and its daughter cells, and to assign gene expression to individual clonal cells. Such lineage-based information is a critical key for understanding the cellular and m作者: 音樂(lè)等 時(shí)間: 2025-3-27 23:42 作者: 瑣碎 時(shí)間: 2025-3-28 02:06
I. Akkerman,K. Benner,Y. Bazilevsompared to other tissues such as muscles or cuticularized body parts, the interpretation in μCT scans is challenging and needs some practice. The protocol described here is also applicable for larger specimens of a variety of species and spans over 2–3?days resulting in an image stack ready for post作者: Range-Of-Motion 時(shí)間: 2025-3-28 10:16
The Search for Life: Past and Present,nd localization during development. Thus, genetic tools and immunohistochemistry are complementary techniques for studying cellular and developmental processes in .. Protocols for immunostaining in . are similar to those in other organisms; however, some features of these nematodes provide unique ch作者: condone 時(shí)間: 2025-3-28 13:31 作者: 預(yù)知 時(shí)間: 2025-3-28 18:04 作者: 可卡 時(shí)間: 2025-3-28 19:51 作者: A保存的 時(shí)間: 2025-3-29 01:23
Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian c reporter lines in which fluorescent proteins are expressed in defined populations of neurons are important tools that can overcome these difficulties. By using membrane-attached fluorescent proteins, such reporter transgenes can identify the complete outline of subsets of neurons or they can highl作者: averse 時(shí)間: 2025-3-29 05:26
Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System. Understanding how organ systems and tissues develop and the molecular underpinnings of the processes involved has become an area of new research. The microscopic anatomy of these organisms is best understood through the systematic use of immunochemistry and in situ hybridization procedures. These 作者: CBC471 時(shí)間: 2025-3-29 07:53 作者: BLANK 時(shí)間: 2025-3-29 11:30 作者: Forehead-Lift 時(shí)間: 2025-3-29 16:51 作者: GROSS 時(shí)間: 2025-3-29 21:34
Flybow to Dissect Circuit Assembly in the , Brain: An Updatesms that control neural circuit development and function. This chapter provides detailed technical information on how to use . variants of the mouse Brainbow-2 system, called Flybow, for stochastic labeling of individual cells or lineages with different fluorescent proteins in one sample. We describ作者: FIG 時(shí)間: 2025-3-30 02:14
Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brainto keep the cells under best physiological condition and to prevent abnormal cellular behavior, which might be caused by phototoxicity during microscopy. In this chapter we describe a protocol to visualize division patterns of neural stem cells in live whole mount brains of . larvae. We also present作者: reception 時(shí)間: 2025-3-30 06:27
CRISPR/Cas9 Genome Editing to Study Nervous System Development in nervous system. While transgenic approaches have been heavily used to investigate how the brain develops, genome editing has been notoriously hard in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to 作者: embolus 時(shí)間: 2025-3-30 10:06 作者: impaction 時(shí)間: 2025-3-30 14:07
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Em genesis is driven by specific gene products whose expression underlies a tight spatiotemporal control. Therefore, the analysis of gene expression in time and space provides valuable insights into the molecular mechanisms that govern brain development. Since .-specific antibodies are scarce, fluores作者: 先驅(qū) 時(shí)間: 2025-3-30 17:05 作者: 低三下四之人 時(shí)間: 2025-3-30 23:59 作者: vocation 時(shí)間: 2025-3-31 01:44 作者: 廚師 時(shí)間: 2025-3-31 08:21
Immunostainings in Nervous System Development of the Nematode nslucence, short life cycle, and abundance of genetic tools (WormBase. ., 2018; WormBook. The C. elegans Research Community. ., 2018). Due to the relative ease of genetic transformation, the majority of studies in . use transgenes (e.g., green fluorescent proteins) to assess the expression and distr作者: Eviction 時(shí)間: 2025-3-31 11:26 作者: 袖章 時(shí)間: 2025-3-31 15:30
