標(biāo)題: Titlebook: Brachypodium Genomics; Methods and Protocol Gaurav Sablok,Hikmet Budak,Peter J. Ralph Book 2018 Springer Science+Business Media LLC 2018 im [打印本頁(yè)] 作者: 租期 時(shí)間: 2025-3-21 17:54
書(shū)目名稱(chēng)Brachypodium Genomics影響因子(影響力)
作者: 外來(lái) 時(shí)間: 2025-3-22 00:15 作者: Genetics 時(shí)間: 2025-3-22 01:30
Sudhir Gupta,Eugene Butcher,William Paulinto grass karyotype evolution. In this protocol we describe the methods of making somatic and meiotic chromosome preparations, probe labeling, FISH with BAC clones, a strategy for chromosome barcoding and chromosome painting in ., and comparative chromosome painting in the other . species.作者: synchronous 時(shí)間: 2025-3-22 04:54 作者: 染色體 時(shí)間: 2025-3-22 12:14
Lymphocytes, Macrophages, and Cancerare available throughout the year in contrast to immature embryos. We explain a tissue culture protocol for . using mature embryos with the selected inbred lines from our collection. Embryogenic calluses obtained from mature embryos are used to transform . with both plant transformation techniques t作者: strain 時(shí)間: 2025-3-22 14:07 作者: CAND 時(shí)間: 2025-3-22 17:15
Identification of Pseudogenes in , Chromosomes,pseudogenes functionally paralogous as the pseudogene paternal locus, (3) uses the intron–exon structure of paternal genes to distinguish between pseudogene types..The pipeline is presented in its composing steps and tested on the . Bd1 scaffold.作者: single 時(shí)間: 2025-3-22 23:56 作者: 緯度 時(shí)間: 2025-3-23 02:05
Review of International Studies of LITassay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible . infection assay for .. We believe this method will help further develop . as a model for genetic improvement of disease resistance in cereals against . pathogens.作者: Biguanides 時(shí)間: 2025-3-23 09:31
Controversies About LIT: REMIS Studys but not during seed transmission. We developed . transgenic plants that can express the . element. Here, we describe an efficient tissue culture-based approach to generate . insertional mutant population using transgenic . line expressing the . retrotransposon.作者: 折磨 時(shí)間: 2025-3-23 12:20 作者: Neolithic 時(shí)間: 2025-3-23 17:40
Lymphocyte Trafficking in Health and Diseaseethods including capillary electrophoresis. Here, we introduce the development of the mutant population BRACHYLIFE and present a Brachypodium TILLING protocol based on fluorescing primers for PCR, enzymatic cleavage, and detection with Applied Biosystems 3130xl Genetic Analyzer.作者: 暴發(fā)戶(hù) 時(shí)間: 2025-3-23 19:36
T Cell Heterogeneity and Function,pproaches towards the whole-genome and transcriptome-assisted simple sequence repeats (SSRs) marker mining in . and identification of orthologus SSRs and their validation in . ecotypes. We also present a protocol for the validation of the identified markers.作者: 搖曳的微光 時(shí)間: 2025-3-23 23:31
Recent Results in Cancer Researchmodules to map these key genes as e-QTLs. High throughput sequencing approaches has revolutionized the mining of the gene behavior and also the association of the genes over time-series. The present protocol chapter presents a unified workflow to understand the transcriptional modules in . using weighted coexpressed gene network analysis approach.作者: 說(shuō)明 時(shí)間: 2025-3-24 03:23
1064-3745 ation advice from the experts.This volume presents protocols for?.Brachypodium.?genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic bas作者: 技術(shù) 時(shí)間: 2025-3-24 06:32
https://doi.org/10.1007/0-387-34132-3polysaccharide structures. Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in ..作者: delegate 時(shí)間: 2025-3-24 12:01 作者: Congregate 時(shí)間: 2025-3-24 17:33 作者: 節(jié)約 時(shí)間: 2025-3-24 21:29
Whole Genome DNA Methylation Analysis of , Using Next-Generation Sequencing (BS-seq), have been widely also demonstrated at the flowering, tissue-specific, and also at developmental stages revealing a strong association of the observed epi-alleles to the physiological state. In the present chapter, epi-genomic analysis of the . has been described with functional workflow and illustratedmethodology.作者: 結(jié)構(gòu) 時(shí)間: 2025-3-25 01:32 作者: 主講人 時(shí)間: 2025-3-25 03:51 作者: 燕麥 時(shí)間: 2025-3-25 09:25
DNA Replication Units in Eukaryotes,e sequence resources for . crops, wheat, and barley. We introduce the functionalities and availability of these important information resources. Furthermore, we also present brief descriptions of useful online tools that facilitate Brachypodium functional genomics studies.作者: 搜尋 時(shí)間: 2025-3-25 14:39
Raffaele Badolato,Silvano Sozzaniaccomplish this protocol, the readers need some basic command-line skills. Briefly, the protocol comprises four major steps: downloading data and software, setting up computing environment, preparing data, and estimating CUB.作者: Root494 時(shí)間: 2025-3-25 17:14 作者: Pathogen 時(shí)間: 2025-3-25 20:59 作者: 尊敬 時(shí)間: 2025-3-26 00:48 作者: 陳列 時(shí)間: 2025-3-26 05:39
Review of International Studies of LITbeen identified both in animal?and plant systems, the regulatory roles of lncRNAs are only beginning to be recognized and the molecular basis of lncRNA mediated gene regulation remains largely unexplored, particularly in plants..Here, we describe an efficient methodology to identify long noncoding RNAs using next-generation sequencing data.作者: CODE 時(shí)間: 2025-3-26 10:09
A. Castellani,C. Catena,G. Biondified conserved AS patterns among monocot and dicot plants. In this chapter, using a Brachypodium-virus pathosystem as a research template, we provide an overview of genomic and bioinformatic tools that can be used to investigate constitutive and alternative splicing in plants.作者: Flat-Feet 時(shí)間: 2025-3-26 15:44
https://doi.org/10.1007/978-3-642-74225-5by?mapping of the smallRNAs taking into account the transcript coordinates. Here, we describe a workflow that can be used for the identification . and corresponding phasiRNAs in . using publically availabe smallRNAs datasets.作者: Chandelier 時(shí)間: 2025-3-26 18:15 作者: QUAIL 時(shí)間: 2025-3-27 00:49
Genomic Approaches to Analyze Alternative Splicing, A Key Regulator of Transcriptome and Proteome Dfied conserved AS patterns among monocot and dicot plants. In this chapter, using a Brachypodium-virus pathosystem as a research template, we provide an overview of genomic and bioinformatic tools that can be used to investigate constitutive and alternative splicing in plants.作者: 玉米 時(shí)間: 2025-3-27 04:42 作者: Trochlea 時(shí)間: 2025-3-27 05:20
A Highly Efficient and Reproducible , spp. Inoculation Method for ,,assay that facilitates quick screening of germplasm (e.g., mutant lines). Here, we provide an efficient and reproducible . infection assay for .. We believe this method will help further develop . as a model for genetic improvement of disease resistance in cereals against . pathogens.作者: instructive 時(shí)間: 2025-3-27 11:45 作者: 衍生 時(shí)間: 2025-3-27 14:00 作者: Goblet-Cells 時(shí)間: 2025-3-27 20:12
TILLING in ,,ethods including capillary electrophoresis. Here, we introduce the development of the mutant population BRACHYLIFE and present a Brachypodium TILLING protocol based on fluorescing primers for PCR, enzymatic cleavage, and detection with Applied Biosystems 3130xl Genetic Analyzer.作者: 急急忙忙 時(shí)間: 2025-3-28 00:04 作者: nonplus 時(shí)間: 2025-3-28 03:31
Protocol for Coexpression Network Construction and Stress-Responsive Expression Analysis in ,,modules to map these key genes as e-QTLs. High throughput sequencing approaches has revolutionized the mining of the gene behavior and also the association of the genes over time-series. The present protocol chapter presents a unified workflow to understand the transcriptional modules in . using weighted coexpressed gene network analysis approach.作者: needle 時(shí)間: 2025-3-28 07:45
Book 2018etabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of?.Brachypodium.?that will help researchers better understand the model plant using NGS technologies. Written in the highly successful?.Methods in Molecular Biology?.series for作者: 有角 時(shí)間: 2025-3-28 13:03 作者: myocardium 時(shí)間: 2025-3-28 15:25 作者: 百科全書(shū) 時(shí)間: 2025-3-28 22:14 作者: 確認(rèn) 時(shí)間: 2025-3-29 00:57
Sudhir Gupta,Eugene Butcher,William Paul (.?=?5) basic number of chromosomes easily identifiable on the basis of their morphometric features. Some of these features, such as genome compactness, are shared by the other members of the genus, thus making them amenable for comparative cytogenetic mapping. Cytogenetic infrastructure establishe作者: adroit 時(shí)間: 2025-3-29 05:51
Fusion of Mouse Myeloma and Spleen Cells,der to survive in suboptimal conditions..Here, we describe a standardized in vivo soil drought assay to investigate the effects of drought stress on leaf growth. Since it is now clear that stress responses can be specific to developmental stages and cell types, we describe a procedure to dissect the作者: 堅(jiān)毅 時(shí)間: 2025-3-29 09:02 作者: invade 時(shí)間: 2025-3-29 12:41 作者: MURAL 時(shí)間: 2025-3-29 17:49 作者: 主講人 時(shí)間: 2025-3-29 20:47
https://doi.org/10.1007/0-387-34132-3les, such as DNA, peptides, and oligosaccharides. This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures. Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypod作者: Gourmet 時(shí)間: 2025-3-30 02:21
A. Castellani,C. Catena,G. Biondiss. Advancements in next-generation sequencing, bioinformatics, and computational biology tools have allowed biologists to investigate AS landscapes on a genome-wide scale in several plant species. Furthermore, the development of . (Brachypodium) as a model system for grasses has facilitated compara作者: 填滿(mǎn) 時(shí)間: 2025-3-30 06:50 作者: accordance 時(shí)間: 2025-3-30 08:13 作者: etidronate 時(shí)間: 2025-3-30 16:09 作者: BLA 時(shí)間: 2025-3-30 18:37
Raffaele Badolato,Silvano Sozzani are often coded by 61 codons, so one amino acid can be coded by more than one codon and the codons coding the same amino acid are called synonymous. Intriguingly, synonymous codons’ usage is often uneven: some are used more often than their alternatives in a genome. The unevenness of codon usage is作者: Prosaic 時(shí)間: 2025-3-31 00:16 作者: 巨碩 時(shí)間: 2025-3-31 01:00 作者: 史前 時(shí)間: 2025-3-31 07:31
https://doi.org/10.1007/978-3-642-74225-5e been shown to be the first dominant class?and has been widely characterized as post-transcriptional regulators. In addition to microRNAs, triggered by miRNAs, transcripts called as . (or .) generate abundant class?of small RNAs in 21-nt manner, which is a pattern formed by DICER-LIKE 4 (DCL4) proc作者: 許可 時(shí)間: 2025-3-31 12:25 作者: 調(diào)整 時(shí)間: 2025-3-31 13:51
Recent Results in Cancer Researche scale gene association. Application of the graph theory and combination of tools has allowed to understand the genic interaction and to understand the role of hub and non-hub proteins in plant development and its ability to cope with stress. Association genetics has also been coupled with network 作者: 繞著哥哥問(wèn) 時(shí)間: 2025-3-31 21:02 作者: 先行 時(shí)間: 2025-4-1 00:48
Lymphocytes, Macrophages, and Cancerits of a model species is its capacity to be transformed and ease of growing both in tissue culture and in greenhouse conditions. Hence, plant transformation technology is crucial for improvements in agricultural studies, both for the study of new genes and in the production of new transgenic plant
