作者: 真實(shí)的你 時(shí)間: 2025-3-21 21:53
Martin G?bl,Andreas Froschmayerdetected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in . has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in .作者: Transfusion 時(shí)間: 2025-3-22 01:37 作者: LUT 時(shí)間: 2025-3-22 05:56
Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue,n the two loci allows for discrimination between strains representing all the areas of distribution. The methods presented here are meant for genotyping directly from ticks and from blood and tissue samples from vertebrates.作者: Etymology 時(shí)間: 2025-3-22 09:04
Identification of Acetylated Proteins in ,,detected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in . has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in .作者: 意外的成功 時(shí)間: 2025-3-22 16:14
,Analysis of , Proteome and Protein–Protein Interactions,identification via liquid chromatography-mass spectrometry and database searching. We also describe assays for studying the interaction between borrelial proteins: a novel high-throughput luciferase assay, yeast two-hybrid assay, and a far-Western assay that are routinely used in our laboratories.作者: indenture 時(shí)間: 2025-3-22 18:44
Herbert Kopfer,Christian Bierwirtherived proteins interacting with complement regulator Factor H is described, including the preparation of whole cell lysates, the separation of proteins by Tris-Tricine SDS-PAGE, the transfer of the proteins to nitrocellulose membranes, and the detection of Factor H-interacting proteins by Far western.作者: 圓桶 時(shí)間: 2025-3-22 23:16
https://doi.org/10.1007/978-3-642-60184-2lates that are attenuated for infection in mice and thus cannot be naturally acquired by ticks. We also describe a quantitative PCR-based protocol for the measurement of . in tick and murine hosts targeting spirochete RNA that is highly efficient, reproducible, and a better surrogate of active infection.作者: BARB 時(shí)間: 2025-3-23 03:20 作者: intrude 時(shí)間: 2025-3-23 07:30
Artificial Infection of Ticks with , Using a Microinjection Method and Their Detection In Vivo Usinlates that are attenuated for infection in mice and thus cannot be naturally acquired by ticks. We also describe a quantitative PCR-based protocol for the measurement of . in tick and murine hosts targeting spirochete RNA that is highly efficient, reproducible, and a better surrogate of active infection.作者: Palatial 時(shí)間: 2025-3-23 10:56 作者: thyroid-hormone 時(shí)間: 2025-3-23 15:19
1064-3745 ation advice from the experts.This volume details protocols that broadly cover many aspects of basic and translational research on?.Borrelia burgdorferi.?.Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, anima作者: 規(guī)范就好 時(shí)間: 2025-3-23 20:58 作者: 字形刻痕 時(shí)間: 2025-3-24 02:08 作者: outrage 時(shí)間: 2025-3-24 05:36 作者: ACTIN 時(shí)間: 2025-3-24 08:07
Schluss: Zusammenfassung und Ausblick,l caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and transform competent cells, select for and isolate transformants, and complement or genetically restore mutants.作者: Aerophagia 時(shí)間: 2025-3-24 11:26
Analysis of Lipids and Lipid Rafts in ,,rafts define the ability of cells to form microdomains and compartmentalize the membrane. In this chapter, we describe the biophysical, biochemical, and molecular approaches used to define and characterize lipid rafts in the Lyme disease agent, ..作者: 瑣碎 時(shí)間: 2025-3-24 16:57
Trace Element Analysis of , by Inductively Coupled Plasma-Sector Field Mass Spectrometry,onutrients and referred to as trace elements. Manganese (Mn), iron (Fe), copper (Cu), and zinc (Zn) are the most prevalent transition metals in the Lyme disease spirochete .. Here, we describe a method for the accurate measurement of these trace elements in . utilizing inductively coupled plasma-sector field mass spectrometry (ICP-SFMS).作者: DRILL 時(shí)間: 2025-3-24 22:20 作者: Constrain 時(shí)間: 2025-3-25 02:19
Genetic Transformation and Complementation,l caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and transform competent cells, select for and isolate transformants, and complement or genetically restore mutants.作者: Expurgate 時(shí)間: 2025-3-25 06:43 作者: 遭受 時(shí)間: 2025-3-25 10:48 作者: Calibrate 時(shí)間: 2025-3-25 14:37
https://doi.org/10.1007/978-3-322-92074-4mutants are also broadly useful for phenotypic analyses of genes that require a tightly regulated and artificially inducible copy of the target gene. Herein, we describe the methods used to generate and confirm conditional mutant clones in . utilizing the .-adapted . operator/repressor system.作者: 偏見(jiàn) 時(shí)間: 2025-3-25 16:51 作者: NAIVE 時(shí)間: 2025-3-25 20:18
Generation of Conditional Mutants in ,,mutants are also broadly useful for phenotypic analyses of genes that require a tightly regulated and artificially inducible copy of the target gene. Herein, we describe the methods used to generate and confirm conditional mutant clones in . utilizing the .-adapted . operator/repressor system.作者: 警告 時(shí)間: 2025-3-26 01:10 作者: Tartar 時(shí)間: 2025-3-26 07:32 作者: 信徒 時(shí)間: 2025-3-26 12:30 作者: AORTA 時(shí)間: 2025-3-26 13:05
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/189902.jpg作者: PLE 時(shí)間: 2025-3-26 17:44
Borrelia burgdorferi978-1-4939-7383-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 真實(shí)的人 時(shí)間: 2025-3-26 23:44 作者: 整體 時(shí)間: 2025-3-27 03:26 作者: corn732 時(shí)間: 2025-3-27 08:27 作者: 金絲雀 時(shí)間: 2025-3-27 11:28 作者: FIN 時(shí)間: 2025-3-27 13:52
Thomas Spengler,Stefan Vo?,Herbert Kopferary. Nowadays, molecular methods are the mainstay for bacterial identification and sequence-based analyses are of ever-growing importance. For diagnostics, immediate results are needed and often real-time PCR of one or two loci is the method of choice while for epidemiological or evolutionary studie作者: ONYM 時(shí)間: 2025-3-27 19:04 作者: 售穴 時(shí)間: 2025-3-27 23:31 作者: 壕溝 時(shí)間: 2025-3-28 04:51 作者: 新奇 時(shí)間: 2025-3-28 07:45 作者: Indecisive 時(shí)間: 2025-3-28 13:50 作者: forager 時(shí)間: 2025-3-28 18:25
Herbert Kopfer,Christian Bierwirth, inhibiting activation of complement as innate immunity’s first line of defense. Hence, the identification and characterization of interacting ligands is a prerequisite to gain deeper insights into the molecular principles of how spirochetes overcome the detrimental effects of complement. Far weste作者: Alveoli 時(shí)間: 2025-3-28 21:55 作者: 明智的人 時(shí)間: 2025-3-29 02:54 作者: 發(fā)電機(jī) 時(shí)間: 2025-3-29 07:07
Martin G?bl,Andreas Froschmayerions of interest. Here, we describe modifications to employ this genome-wide screening method for ., the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the . transcriptome during infection, despite the low bacterial numbers i作者: 火海 時(shí)間: 2025-3-29 08:50
Martin G?bl,Andreas Froschmayerbind DNA or RNA in a given prokaryote. However, biological relevance of these regulatory proteins requires methods to rapidly purify and determine their binding properties within the physiological context or life style of the organism. Here, we describe the experimental approaches to determine the n作者: Thyroid-Gland 時(shí)間: 2025-3-29 13:03
Martin G?bl,Andreas Froschmayerne acetylation—the acetylation of the epsilon amino group of lysine residues. Nε-lysine acetylation is known to regulate multiple cellular processes. In eukaryotes, it regulates chromatin structure, transcription, metabolism, signal transduction, and the cytoskeleton. Recently, multiple groups have 作者: 內(nèi)部 時(shí)間: 2025-3-29 18:42
Schluss: Zusammenfassung und Ausblick,tants in these recalcitrant bacteria, while not straightforward, is routinely accomplished in numerous laboratories, although there are several crucial caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and t作者: FLEET 時(shí)間: 2025-3-29 22:57 作者: 能得到 時(shí)間: 2025-3-30 01:24
https://doi.org/10.1007/978-3-322-92074-4erate deletion strains lacking the gene of interest. To circumvent this limitation, it is possible to generate conditional mutants in which a copy of the gene of interest is introduced into the bacteria to compensate for the loss of the native allele. Expression of the non-native copy of the target 作者: Trabeculoplasty 時(shí)間: 2025-3-30 06:32 作者: 完成才會(huì)征服 時(shí)間: 2025-3-30 11:59
https://doi.org/10.1007/978-3-658-23805-6of . proteome and protein–protein interactions, which play central roles in biological processes in diverse organisms, are critical in understanding biology and infectivity of spirochetes. Here, we describe the proteomic analysis of . by two-dimensional (2-D) gel electrophoresis followed by protein 作者: antecedence 時(shí)間: 2025-3-30 13:22 作者: 預(yù)兆好 時(shí)間: 2025-3-30 19:49 作者: NICHE 時(shí)間: 2025-3-30 23:06
Co-immunoprecipitation for Identifying Protein-Protein Interactions in ,,co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete .. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.作者: Corral 時(shí)間: 2025-3-31 03:43 作者: Mercurial 時(shí)間: 2025-3-31 07:39 作者: cavity 時(shí)間: 2025-3-31 12:00
1064-3745 thoritative and cutting-edge,?.Borrelia burgdorferi:Methods and Protocols?.aims to ensure successful results in the further study of this vital field of biomedical research..978-1-4939-8468-8978-1-4939-7383-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: HERE 時(shí)間: 2025-3-31 15:18
Species Identification and Phylogenetic Analysis of , Sensu Lato Using Molecular Biological Methodsecies complex, the importance of accurate species and strain typing has come to the fore. This is particularly significant with a view of differentiating human pathogenic and non-pathogenic strains or species and understanding the epidemiology, ecology, population structure, and evolution of species作者: 血友病 時(shí)間: 2025-3-31 21:10
Analysis of DNA and RNA Binding Properties of , Regulatory Proteins,escribed. The methods described are intended to facilitate empirical assessment of the binding affinity, co-factor requirements, quality of the interacting partners, and readily modifiable assay conditions to assess the binding properties to define known and unknown regulatory properties of nucleic 作者: interrupt 時(shí)間: 2025-4-1 00:48
Genome-Wide Mutagenesis in ,,rent time points using Luminex Flex.Map? technology provides a robust data set. . transposon mutant screening indicates that a high proportion of genes are the novel virulence determinants that are required for mouse and tick infection. In this protocol, an effective signature-tagged .-based transpo作者: 單調(diào)女 時(shí)間: 2025-4-1 03:35 作者: chlorosis 時(shí)間: 2025-4-1 09:49
Thomas Spengler,Stefan Vo?,Herbert Kopferecies complex, the importance of accurate species and strain typing has come to the fore. This is particularly significant with a view of differentiating human pathogenic and non-pathogenic strains or species and understanding the epidemiology, ecology, population structure, and evolution of species作者: Esophagitis 時(shí)間: 2025-4-1 12:30 作者: CRACY 時(shí)間: 2025-4-1 17:26
https://doi.org/10.57088/978-3-7329-9093-1rent time points using Luminex Flex.Map? technology provides a robust data set. . transposon mutant screening indicates that a high proportion of genes are the novel virulence determinants that are required for mouse and tick infection. In this protocol, an effective signature-tagged .-based transpo作者: spondylosis 時(shí)間: 2025-4-1 21:01
Siegfried Augustin,Christian Frühwaldce. Molecular genetic techniques to evaluate and quantitate . infection are laborious and costly. To partly circumvent these issues, a codon optimized firefly luciferase, under the control of a constitutive borrelial promoter, was introduced into . enabling the characterization of mutant or modified
