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標(biāo)題: Titlebook: Bone Research Protocols; Miep H. Helfrich,Stuart H. Ralston Book 20031st edition Humana Press 2003 [打印本頁(yè)]

作者: 冰凍    時(shí)間: 2025-3-21 19:22
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作者: 索賠    時(shí)間: 2025-3-21 22:54
https://doi.org/10.1007/978-3-642-58641-5es, and antigenic profiles (.–.). Most importantly, they develop a capacity for bone pit resorption, the unique and defining functional attribute of OCs. Bone resorption and formation are normally carefully balanced processes in adults. However, in various diseases or pathological conditions, an imb
作者: Cytokines    時(shí)間: 2025-3-22 04:24

作者: 匍匐    時(shí)間: 2025-3-22 04:58

作者: Estrogen    時(shí)間: 2025-3-22 11:00
J. Pettavel,S. Leyvraz,P. Douglas mature OCs exist and the few pre-OC cell lines that have been reported either do not undergo full OC differentiation (.,.) or involve coculture systems and cells that may not be readily available to all researchers (.–.). To compound the problem further, it has proven difficult or impossible until
作者: 偽造者    時(shí)間: 2025-3-22 13:49
Cornelis J. H. Velde,Paul H. Sugarbakernd human .. More recently, techniques have been developed to generate osteoclasts in vitro (.,.) and then incubate these on bone slices to produce bone resorption. With these approaches, osteoclasts are defined by their ability to produce resorption pits on bone slices. However, this definition may
作者: reject    時(shí)間: 2025-3-22 17:24
Mineralizing Fibroblast-Colony-Forming Assaysxogenous agents in a similar manner to that observed in vivo (.). Recently a number of in vitro models have been established that re-create discrete elements of the cellular network present in the bone micro-environment. The advantage of these models is that they have reduced complexity compared wit
作者: certitude    時(shí)間: 2025-3-23 00:30
Primary Isolation and Culture of Chicken Osteoclastses, and antigenic profiles (.–.). Most importantly, they develop a capacity for bone pit resorption, the unique and defining functional attribute of OCs. Bone resorption and formation are normally carefully balanced processes in adults. However, in various diseases or pathological conditions, an imb
作者: 沉思的魚    時(shí)間: 2025-3-23 04:31

作者: 玩忽職守    時(shí)間: 2025-3-23 06:26
Osteoclast Formation in the Mouse Coculture Assay interactions. It has been widely used to study the origin of the osteoclast (.) and the effects of growth factors and drugs on osteoclast formation (.,.). In studies with osteopetrotic mice, the coculture assay has been used to determine whether the underlying mechanism was due to a defect in the o
作者: 伸展    時(shí)間: 2025-3-23 11:08
RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells mature OCs exist and the few pre-OC cell lines that have been reported either do not undergo full OC differentiation (.,.) or involve coculture systems and cells that may not be readily available to all researchers (.–.). To compound the problem further, it has proven difficult or impossible until
作者: 泥沼    時(shí)間: 2025-3-23 15:50

作者: 熟練    時(shí)間: 2025-3-23 21:09

作者: 使入迷    時(shí)間: 2025-3-24 02:10

作者: pantomime    時(shí)間: 2025-3-24 04:25

作者: 他一致    時(shí)間: 2025-3-24 10:32

作者: 袋鼠    時(shí)間: 2025-3-24 13:29
Assessing Bone Formation Using Mouse Calvarial Organ Culturesrtisone (.) and bisphosphonates (.). Others have used rodent calvarial and long bone explants to study the effects of cytokines on bone resorption (.–.) and bone formation (.,.). This chapter focuses on the use of cultured neonatal calvariae as an assay for agents with anabolic activity (.–.) (..).
作者: 拉開這車床    時(shí)間: 2025-3-24 18:49
Shahid M. Hussain,Michael F. Sorrellines appear unstable to some extent. In addition, their clonal selection has favored rapidly growing cells, but has not necessarily selected for the whole range of bone-specific gene expression characteristic of primary bone cells. This means that in certain experiments, the use of primary bone cells is preferred to the use of cell lines.
作者: 暗指    時(shí)間: 2025-3-24 22:40
https://doi.org/10.1007/978-3-642-58641-5tes were outside the mainstream of bone research. Increasing interest in the mechanoregulation of bone has changed this, and today there is a general consensus that osteocytes play a pivotal role as mechanosensors and effectors in bone (.). Whether osteocytes have other functions remains to be elucidated.
作者: 得罪人    時(shí)間: 2025-3-24 23:20
Bruno F. Fernandes,Miguel N. Burnier Jrrtisone (.) and bisphosphonates (.). Others have used rodent calvarial and long bone explants to study the effects of cytokines on bone resorption (.–.) and bone formation (.,.). This chapter focuses on the use of cultured neonatal calvariae as an assay for agents with anabolic activity (.–.) (..).
作者: 飛行員    時(shí)間: 2025-3-25 06:19
https://doi.org/10.1007/978-3-642-58641-5last biology. Data from such short term cultures complements that obtained from bone organ culture resorption models and long-term cultures of osteoclast forming hematopoietic stem cells derived from marrow or peripheral blood.
作者: amnesia    時(shí)間: 2025-3-25 10:28

作者: 本土    時(shí)間: 2025-3-25 12:50
Isolated Osteoclast Cultureslast biology. Data from such short term cultures complements that obtained from bone organ culture resorption models and long-term cultures of osteoclast forming hematopoietic stem cells derived from marrow or peripheral blood.
作者: Ovulation    時(shí)間: 2025-3-25 16:05
Isolation and Purification of Rabbit Osteoclastsoclasts for studies on metabolic processes in osteoclasts, or molecular studies on osteoclast biology using Western blotting, enzyme assays or reverse transcriptase-polymerase chain reaction (RT-PCR) (.–.).
作者: nuclear-tests    時(shí)間: 2025-3-25 20:43
Book 20031st editionrders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. Thi
作者: Limousine    時(shí)間: 2025-3-26 03:05
1543-1894 bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on
作者: 600    時(shí)間: 2025-3-26 04:23

作者: 柱廊    時(shí)間: 2025-3-26 10:37

作者: 裝入膠囊    時(shí)間: 2025-3-26 15:54

作者: 大包裹    時(shí)間: 2025-3-26 18:22
Surgery for Breast Cancer Liver Metastases,her calcified bone or dentine but not with plastic or uncalcified collagen-based matrices. This subcellular space is dependent upon the formation of a “tight seal” by the osteoclast, a process involving rearrangement of the cytoskeleton into a characteristic F-actin ring structure (. the chapter by Nesbitt and Horton, ., for details).
作者: 恃強(qiáng)凌弱的人    時(shí)間: 2025-3-26 21:41
Generating Human Osteoclasts from Peripheral Bloodarrow precursor (.). These studies included parabiosis experiments in which normal and affected (osteopetrotic/radiation-treated) littermates are linked by a common circulation; these experiments established that the mononuclear osteoclast precursor is present in peripheral blood (.,.).
作者: Blanch    時(shí)間: 2025-3-27 02:10
Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Bloodher calcified bone or dentine but not with plastic or uncalcified collagen-based matrices. This subcellular space is dependent upon the formation of a “tight seal” by the osteoclast, a process involving rearrangement of the cytoskeleton into a characteristic F-actin ring structure (. the chapter by Nesbitt and Horton, ., for details).
作者: 喪失    時(shí)間: 2025-3-27 08:53
Methods in Molecular Medicinehttp://image.papertrans.cn/b/image/189708.jpg
作者: 體貼    時(shí)間: 2025-3-27 11:02
Human Osteoblast Cultureequent mineralization. Once a phase of active bone formation is completed the osteoblasts do not become senescent but instead redifferentiate into one of two other cell types: osteocytes and bone lining cells, both of which play a major role in the regulation of calcium homeostasis and bone remodeling.
作者: Mercurial    時(shí)間: 2025-3-27 14:24

作者: 使虛弱    時(shí)間: 2025-3-27 19:34
Biliary Hamartomas (von Meyenberg Complexes)equent mineralization. Once a phase of active bone formation is completed the osteoblasts do not become senescent but instead redifferentiate into one of two other cell types: osteocytes and bone lining cells, both of which play a major role in the regulation of calcium homeostasis and bone remodeling.
作者: 知識(shí)分子    時(shí)間: 2025-3-27 22:26
Biliary Hamartomas (von Meyenberg Complexes)equent mineralization. Once a phase of active bone formation is completed the osteoblasts do not become senescent but instead redifferentiate into one of two other cell types: osteocytes and bone lining cells, both of which play a major role in the regulation of calcium homeostasis and bone remodeli
作者: 松軟    時(shí)間: 2025-3-28 05:06

作者: aquatic    時(shí)間: 2025-3-28 07:05
Shahid M. Hussain,Michael F. Sorrellrix. These cells interact with each other and with the extracellular matrix, and when cell populations are removed from the network they cease to function normally. In the past, bone cell differentiation was studied using histological methods in either whole embryos or organ cultures. Although this
作者: fluoroscopy    時(shí)間: 2025-3-28 13:40

作者: 拍翅    時(shí)間: 2025-3-28 14:44

作者: Vasoconstrictor    時(shí)間: 2025-3-28 20:21
https://doi.org/10.1007/978-3-642-58641-5es: bone-resorbing osteoclasts (OCs) which are uniquely capable of dissolving and removing a small volume of bone, and bone-forming osteoblasts that subsequently fill in these lacunae or pits with new bone tissue. Whereas osteoblasts originate from mesenchymal cell precursors, OCs derive from hemato
作者: 無(wú)孔    時(shí)間: 2025-3-29 02:58

作者: Commentary    時(shí)間: 2025-3-29 04:49
Cryotherapy and Other Ablative Procedures, osteoclasts were thought to share a common progenitor cell with osteoblasts, bone-forming cells. Osteoclast formation occurring as a consequence of fusion of nonosteoblastic mononuclear precursor cells was suggested by a number of early investigators including Pommer (1883), Mallory (1912), La Cost
作者: 媒介    時(shí)間: 2025-3-29 10:25
Surgery for Breast Cancer Liver Metastases,bunit of the vitronectin receptor (VNR) and the calcitonin receptor (CTR). The extracellular degradative process, known as bone resorption, is the hallmark of the osteoclast, and includes removal of both the hydroxyapatite and organic components of the skeleton. For bone resorption to occur, osteocl
作者: enchant    時(shí)間: 2025-3-29 13:18
Gastrointestinal Stromal Tumors,oped a mouse bone marrow culture system, in which osteoclasts are formed in response to several bone-resorbing factors such as 1α,25-dihydroxyvitamin D. [1α,25-(OH).D.], parathyroid hormone (PTH), prostaglandin E. (PGE.) and interleukin-11 (IL-11) (.,.). We also developed a mouse coculture system of
作者: 侵略主義    時(shí)間: 2025-3-29 16:55
Cornelis J. H. Velde,Paul H. Sugarbaker osteoclasts. In this assay, osteoblasts are stimulated with 1,25-dihydroxyvitamin D. (D3) to stimulate RANKL and macrophage colony-stimulating factor (M-CSF) expression. These factors then stimulate early osteoclast precursors present in the spleen or bone marrow cell populations to differentiate i
作者: 畏縮    時(shí)間: 2025-3-29 21:54

作者: 仲裁者    時(shí)間: 2025-3-30 01:25

作者: habile    時(shí)間: 2025-3-30 05:44
Bruno F. Fernandes,Miguel N. Burnier Jrole in bone mineralization based on studies of chick bone fragments. Subsequently, Reynolds used bone explants to study collagen synthesis in bone (.) and to investigate the effects of a wide variety of other agents on bone turnover such as vitamin A (.), ascorbic acid (.), calcitonin (.,.), hydroco
作者: Lignans    時(shí)間: 2025-3-30 12:11

作者: 畫布    時(shí)間: 2025-3-30 12:25
Human Osteoblast Cultureequent mineralization. Once a phase of active bone formation is completed the osteoblasts do not become senescent but instead redifferentiate into one of two other cell types: osteocytes and bone lining cells, both of which play a major role in the regulation of calcium homeostasis and bone remodeli
作者: dysphagia    時(shí)間: 2025-3-30 16:44

作者: Flavouring    時(shí)間: 2025-3-30 21:01
Mineralizing Fibroblast-Colony-Forming Assaysrix. These cells interact with each other and with the extracellular matrix, and when cell populations are removed from the network they cease to function normally. In the past, bone cell differentiation was studied using histological methods in either whole embryos or organ cultures. Although this
作者: A保存的    時(shí)間: 2025-3-31 04:04

作者: 砍伐    時(shí)間: 2025-3-31 06:04
Isolated Osteoclast Cultures the cells responsible for resorbing bone. Osteoclasts are usually few in number relative to other cell types in bone and are difficult to isolate because they are contained in a hard tissue; in addition, they are at the end of their proliferation and differentiation cycle, presenting problems for t
作者: 愚笨    時(shí)間: 2025-3-31 12:28
Primary Isolation and Culture of Chicken Osteoclastses: bone-resorbing osteoclasts (OCs) which are uniquely capable of dissolving and removing a small volume of bone, and bone-forming osteoblasts that subsequently fill in these lacunae or pits with new bone tissue. Whereas osteoblasts originate from mesenchymal cell precursors, OCs derive from hemato
作者: 哥哥噴涌而出    時(shí)間: 2025-3-31 16:57





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