標題: Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe [打印本頁] 作者: 搭話 時間: 2025-3-21 17:10
書目名稱Bone Marrow Environment影響因子(影響力)
書目名稱Bone Marrow Environment影響因子(影響力)學科排名
書目名稱Bone Marrow Environment網(wǎng)絡公開度
書目名稱Bone Marrow Environment網(wǎng)絡公開度學科排名
書目名稱Bone Marrow Environment被引頻次
書目名稱Bone Marrow Environment被引頻次學科排名
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書目名稱Bone Marrow Environment年度引用學科排名
書目名稱Bone Marrow Environment讀者反饋
書目名稱Bone Marrow Environment讀者反饋學科排名
作者: Entrancing 時間: 2025-3-21 23:14 作者: 斗爭 時間: 2025-3-22 00:55
Intravital Imaging of Bone Marrow Microenvironment in the Mouse Calvaria and Tibiae surgical approach, and introduce a bone shaving-based tibial imaging as a complementary method. To demonstrate the applicability of our method we used Lyz2-EGFP transgenic mice to track bone marrow neutrophil activities as an example.作者: infarct 時間: 2025-3-22 07:59
Literature and the Authority of Technology,genitor cells remains essential in the analysis of OCL biology, underlining the importance of reliable gold standard protocols to be applied throughout OCL research. This chapter will deal with in vitro differentiation of OCLs from murine bone marrow cells, as well as isolated monocytes and dendriti作者: 不容置疑 時間: 2025-3-22 10:32
Analysing Literature through Filmshree dimensions at high resolution. Here, we provide a detailed experimental protocol for the generation of thick slices of BM from murine femoral cavities, the immunostaining of cellular and structural components within these samples, and their optical clearing, which enhances the depth at which op作者: CURB 時間: 2025-3-22 14:13 作者: Isolate 時間: 2025-3-22 18:29
1064-3745 d cutting-edge,?.Bone Marrow Environment: Methods and Protocols .aims to help?new investigators to pursue the characterization of the BM microenvironment in the coming years..978-1-0716-1427-3978-1-0716-1425-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: GULF 時間: 2025-3-22 21:47 作者: 陰郁 時間: 2025-3-23 03:37 作者: 使?jié)M足 時間: 2025-3-23 05:33
Willie van Peer,Aikaterini Nousipermitted in-depth studies of HSC niches in mice. Here, we describe our protocol for visualizing and analyzing the localization, morphology, and function of niche components in the mouse calvarium, using combined confocal and two-photon intravital microscopy, and we present the specific example of measuring vascular permeability.作者: 踉蹌 時間: 2025-3-23 13:16
Intrafemoral Delivery of Hematopoietic Progenitorsvironment. As a demonstrative example, we provide a protocol for the isolation of granulocyte–monocyte progenitors (GMP) by cell sorting, the delivery of these cells into recipient animals by intrafemoral transfer, and finally, the analysis of GMP-derived progenies by flow cytometry.作者: 酷熱 時間: 2025-3-23 14:50 作者: tenuous 時間: 2025-3-23 20:24
Book 2021arrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful .Methods in Mol作者: 藐視 時間: 2025-3-23 23:46
https://doi.org/10.1007/978-94-017-4851-3nd respiratory capacity in a single experiment. Here we describe the protocol used to study concomitantly the respiratory and glycolytic metabolism of primary MSCs from the determination of oxygen consumption (OCR) and extracellular acidification (ECAR) rates.作者: 蛤肉 時間: 2025-3-24 03:13
https://doi.org/10.1007/978-94-010-2770-0tive oxygen species and low mitochondrial activity..Here, we describe the methodology to characterize the physiologic state of HSPCs isolated from their native hematopoietic organ using flow cytometry-based assays. These protocols allow evaluation of their ROS levels and activated signaling pathways under various conditions.作者: CERE 時間: 2025-3-24 09:46 作者: Isolate 時間: 2025-3-24 10:40 作者: 千篇一律 時間: 2025-3-24 18:40
Literature and the Authority of Technology,nally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.作者: Osteoporosis 時間: 2025-3-24 19:28
https://doi.org/10.1007/978-94-017-4851-3s. Here we describe the minimum requirements and an efficient method for isolation, expansion of mouse bone-derived multipotent mesenchymal stromal cells and their differentiation into osteoblasts, responsible for the bone matrix synthesis and mineralization.作者: 澄清 時間: 2025-3-24 23:59
https://doi.org/10.1007/978-94-017-4851-3 an original in vitro system allowing to quantify by flow cytometry the differentiation of mouse HSCs into lineage-primed multipotent hematopoietic progenitors (MPPs) in a cytokine-supplemented feeder-free medium.作者: 沉思的魚 時間: 2025-3-25 03:20
https://doi.org/10.1007/978-94-010-2770-0ation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.作者: 四牛在彎曲 時間: 2025-3-25 10:51
Willie van Peer,Aikaterini Nousit bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.作者: 積極詞匯 時間: 2025-3-25 14:20
Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cellsnally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.作者: 出血 時間: 2025-3-25 18:07
Culture, Expansion and Differentiation of Mouse Bone-Derived Mesenchymal Stromal Cellss. Here we describe the minimum requirements and an efficient method for isolation, expansion of mouse bone-derived multipotent mesenchymal stromal cells and their differentiation into osteoblasts, responsible for the bone matrix synthesis and mineralization.作者: 身心疲憊 時間: 2025-3-25 23:42 作者: 放逐某人 時間: 2025-3-26 00:32 作者: Resign 時間: 2025-3-26 05:34
Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrowt bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.作者: SYN 時間: 2025-3-26 10:23 作者: 黑豹 時間: 2025-3-26 13:28
https://doi.org/10.1007/978-94-010-2770-0ate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental characteristics of these diverse cell populations.作者: 珠寶 時間: 2025-3-26 19:52
Introduction: the Mirror and the Image,treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment.作者: Vertebra 時間: 2025-3-26 23:03 作者: decode 時間: 2025-3-27 04:48 作者: 無價值 時間: 2025-3-27 05:36 作者: 熟練 時間: 2025-3-27 12:09
Imaging of Bone Marrow Plasma Cells and of Their Nichesheir interaction with other cell types in situ. We achieved to successfully establish longitudinal imaging of the bone marrow (LIMB) that is based on an implantable endoscopic device. In this chapter, basic approaches on how to investigate plasma cell–stroma interaction and surgical implantation procedures are introduced.作者: 好忠告人 時間: 2025-3-27 17:14
Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cellsscovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling time evaluation will be developed. Fi作者: rectum 時間: 2025-3-27 19:35 作者: 繼承人 時間: 2025-3-27 22:51 作者: Anterior 時間: 2025-3-28 04:28
Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cellslecular components required for their lifelong maintenance and differentiation. Although HSCs have been extensively analyzed and characterized, their ex vivo expansion, which constitutes a promising approach for therapeutic development in regenerative medicine, remains challenging. Here, we describe作者: 煤渣 時間: 2025-3-28 08:37 作者: Brain-Imaging 時間: 2025-3-28 12:29
Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cellsze subsets of hematopoietic stem and progenitor cells isolated from long bones of mice. We further show that this method allows for comparing JAM-C protein expression between subsets of hematopoietic stem and progenitor cells.作者: Interlocking 時間: 2025-3-28 18:18 作者: 愚笨 時間: 2025-3-28 20:58
Immunophenotyping of the Medullary B Cell Compartment In Mouse Modelsce in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopul作者: atrophy 時間: 2025-3-28 23:21 作者: Ornithologist 時間: 2025-3-29 06:48
Multicolor Immunofluorescence Staining of Paraffin-Embedded Human Bone Marrow Sectionsaraffin-embedded (FFPE) tissue sections of human bone marrow. However, the procedure shows technical limitations because of the chemical and physical treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow 作者: 凹槽 時間: 2025-3-29 08:56 作者: VEN 時間: 2025-3-29 12:48 作者: 引起痛苦 時間: 2025-3-29 17:32 作者: 大包裹 時間: 2025-3-29 22:40 作者: Offbeat 時間: 2025-3-30 03:58 作者: 清晰 時間: 2025-3-30 06:45 作者: 有限 時間: 2025-3-30 10:47
Bone Marrow Environment978-1-0716-1425-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 連鎖 時間: 2025-3-30 15:52 作者: arrhythmic 時間: 2025-3-30 18:29 作者: 發(fā)酵劑 時間: 2025-3-30 23:23 作者: 南極 時間: 2025-3-31 02:03
https://doi.org/10.1007/978-94-017-4851-3n differentiate in a variety of cell types such as osteoblasts, chondrocytes and adipocytes. The isolation of MSCs has been carried out by many studies that aim to control their differentiation into cartilaginous and bone cells in vitro in order to use this technology in the repair of damaged tissue作者: Migratory 時間: 2025-3-31 08:17
https://doi.org/10.1007/978-94-017-4851-3lecular components required for their lifelong maintenance and differentiation. Although HSCs have been extensively analyzed and characterized, their ex vivo expansion, which constitutes a promising approach for therapeutic development in regenerative medicine, remains challenging. Here, we describe作者: 強所 時間: 2025-3-31 10:36 作者: 切掉 時間: 2025-3-31 13:28 作者: 慷慨不好 時間: 2025-3-31 20:36
https://doi.org/10.1007/978-94-010-2770-0rvest a good quantity of BMSCs with good viability using fluorescence-activated cell sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncov作者: 過濾 時間: 2025-3-31 22:37 作者: 值得 時間: 2025-4-1 02:07
