標(biāo)題: Titlebook: Bioreactors in Stem Cell Biology; Methods and Protocol Kursad Turksen Book 2016 Springer Science+Business Media New York 2016 Biomaterials. [打印本頁] 作者: CRUST 時間: 2025-3-21 18:36
書目名稱Bioreactors in Stem Cell Biology影響因子(影響力)
書目名稱Bioreactors in Stem Cell Biology影響因子(影響力)學(xué)科排名
書目名稱Bioreactors in Stem Cell Biology網(wǎng)絡(luò)公開度
書目名稱Bioreactors in Stem Cell Biology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioreactors in Stem Cell Biology被引頻次
書目名稱Bioreactors in Stem Cell Biology被引頻次學(xué)科排名
書目名稱Bioreactors in Stem Cell Biology年度引用
書目名稱Bioreactors in Stem Cell Biology年度引用學(xué)科排名
書目名稱Bioreactors in Stem Cell Biology讀者反饋
書目名稱Bioreactors in Stem Cell Biology讀者反饋學(xué)科排名
作者: 摸索 時間: 2025-3-21 22:36 作者: 檢查 時間: 2025-3-22 02:16 作者: investigate 時間: 2025-3-22 07:21 作者: 安裝 時間: 2025-3-22 11:39 作者: 安裝 時間: 2025-3-22 15:12
Gabriel Flynn,David Smith,Mary Kirwanase of up to 4.2 over a period of 5 days compared to a maximum 1.5–2-fold increase in the adherent static culture over a 1 week period. In the bioreactor culture, these cells maintained self-renewal, karyotype stability, and cloning efficiency capabilities. This approach can be also used for human n作者: Matrimony 時間: 2025-3-22 18:39
Multicompartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture,livers oxygen and carbon dioxide for pH regulation. Such bioreactors are available at various dimensions ranging from 0.2 to 800?mL cell compartment volumes (manufactured by StemCell Systems, Berlin, Germany). Here, we describe in detail the setup and maintenance of a small-scale 4-chamber bioreacto作者: 脫落 時間: 2025-3-22 23:56
Uniform Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Steissue development and organogenesis. This chapter describes a protocol for using a rotary suspension bioreactor to produce embryoid bodies and process the differentiation of mouse embryonic stem cells (mESCs), and to assess the efficiency of EB differentiation in the bioreactor by real-time PCR and 作者: AMPLE 時間: 2025-3-23 04:59
Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment,andling. Here, we describe the use of stirred suspension bioreactors, as a novel system for enriching undifferentiated germ cells from 1-week-old pigs. This method capitalizes on the adherent properties of somatic cells within a controlled environment, thus promoting the enrichment of progenitor cel作者: Receive 時間: 2025-3-23 08:46 作者: 蕨類 時間: 2025-3-23 10:54
Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells,in 3D cultures have great potential as complementary tools in preclinical research, bridging the gap between human clinical studies and animal models. The development of robust and scalable processes for the 3D differentiation of hNSC can improve the accuracy of early stage development in preclinica作者: conquer 時間: 2025-3-23 15:32 作者: Osteoarthritis 時間: 2025-3-23 20:54 作者: 預(yù)知 時間: 2025-3-24 01:20 作者: UTTER 時間: 2025-3-24 05:36
https://doi.org/10.1007/978-3-031-28967-5based on the presentation of pertinent markers are also presented. Spinner flask culture with its relatively low capital and operating costs is appealing to laboratories interested in scaling up their production of stem/progenitor cells.作者: 莊嚴(yán) 時間: 2025-3-24 08:47
People in Business: Context and Charactermmonly used in large scale expansion of adherent cells. This chapter describes a detailed culture protocol for hMSC expansion in a 125?mL spinner flask using microcarriers, Cytodex I, and a procedure for cell seeding, expansion, metabolic sampling, and quantification and visualization using microculture tetrazolium (MTT) reagent.作者: 反復(fù)拉緊 時間: 2025-3-24 12:10
Corporate Culture and Organisational Ethicsd differentiation of MSCs, including the procedures to monitor the proliferation, metabolic status and phenotype of MSCs during suspension culture. Moreover, this work proposes suitable materials for cGMP compliant culture conditions enabling the clinical grade production of MSCs.作者: 責(zé)問 時間: 2025-3-24 18:24 作者: GOAD 時間: 2025-3-24 19:30
Monica Moran,Carole Steketee,Kirsty Marlesicroenvironment. The bioreactor platform used in these experiments also allows the implementation of physiologically relevant mechanical signals. Here, we describe a method to build an in vitro model of Ewing’s sarcoma that mimics the key properties of the native tumor and provides the tissue context and physical regulatory signals.作者: Essential 時間: 2025-3-25 01:28
Dawn Forman,Marion Jones,Jill Thistlethwaiteerentiation. We also showed that bundles of axons formed and extended into the microchannels..Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.作者: geriatrician 時間: 2025-3-25 06:38 作者: Cursory 時間: 2025-3-25 08:17 作者: 急性 時間: 2025-3-25 14:26
Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor,mmonly used in large scale expansion of adherent cells. This chapter describes a detailed culture protocol for hMSC expansion in a 125?mL spinner flask using microcarriers, Cytodex I, and a procedure for cell seeding, expansion, metabolic sampling, and quantification and visualization using microculture tetrazolium (MTT) reagent.作者: hangdog 時間: 2025-3-25 17:07 作者: linear 時間: 2025-3-25 19:58
Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading,(3) tissue processing post-harvest for gene expression analysis. These methods may be applied for a variety of applications including graft production, cell priming prior to transplantation and basic investigations of tendon cell biology.作者: 松馳 時間: 2025-3-26 00:57
Bioengineered Models of Solid Human Tumors for Cancer Research,icroenvironment. The bioreactor platform used in these experiments also allows the implementation of physiologically relevant mechanical signals. Here, we describe a method to build an in vitro model of Ewing’s sarcoma that mimics the key properties of the native tumor and provides the tissue context and physical regulatory signals.作者: insular 時間: 2025-3-26 05:17
Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neerentiation. We also showed that bundles of axons formed and extended into the microchannels..Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.作者: 邊緣帶來墨水 時間: 2025-3-26 09:18 作者: 我不重要 時間: 2025-3-26 13:18
https://doi.org/10.1007/978-1-4020-8429-4f combining microcarriers with the controllability of bioreactors to generate higher cell densities in smaller reactor volumes leading to a more efficient and cost-effective cell production for applications in regenerative medicine.作者: Inexorable 時間: 2025-3-26 19:58
Bioreactor Expansion of Skin-Derived Precursor Schwann Cells,f combining microcarriers with the controllability of bioreactors to generate higher cell densities in smaller reactor volumes leading to a more efficient and cost-effective cell production for applications in regenerative medicine.作者: 良心 時間: 2025-3-26 22:54
1064-3745 sults.Contains key notes and implementation advice from the This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs.? No作者: 謙卑 時間: 2025-3-27 04:29 作者: Deduct 時間: 2025-3-27 08:44
Jill Thistlethwaite,Marion Jones investigated models to the process of whole-organ tissue engineering. Hereby, we present the first bioreactor system that allows the integrated 3D biomechanical stimulation of a whole-heart construct while allowing for simultaneous controlled perfusion of the coronary system.作者: 口訣法 時間: 2025-3-27 11:10
Duncan Reid,Marion Jones,Daniel O’Brienn the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining.作者: MERIT 時間: 2025-3-27 14:02 作者: sigmoid-colon 時間: 2025-3-27 20:52 作者: hair-bulb 時間: 2025-3-27 22:45 作者: 壁畫 時間: 2025-3-28 02:18
Gabriel Flynn,Patricia H. Werhaneulture experiments. Here, we describe the two manufacturing techniques photolithography and soft lithography that are used in order to easily produce microfluidic bioreactors. The use of these bioreactors is exemplified by a toxicity assessment on 3D clustered human pluripotent stem cells (hPSC)-derived cardiomyocytes by beating frequency imaging.作者: exceed 時間: 2025-3-28 07:20
Leadership Development Strategies,ardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.作者: 避開 時間: 2025-3-28 10:43
Book 2016dies, not only to large quantities of cells but also to heterogeneous cell constructs.? Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem c作者: 允許 時間: 2025-3-28 17:57 作者: CAJ 時間: 2025-3-28 21:28 作者: trigger 時間: 2025-3-29 02:35
https://doi.org/10.1007/978-1-4939-6478-9Biomaterials; Stem cells; Regenerative medicine; Tissue and organ repair; Heterogeneous cell constructs作者: 聽覺 時間: 2025-3-29 04:05
978-1-4939-8213-4Springer Science+Business Media New York 2016作者: KEGEL 時間: 2025-3-29 07:42
https://doi.org/10.1007/978-3-031-08053-1ich cannot be achieved in conventional static two-dimensional petri dish culture. In cultures of tissue-density this problem can be addressed by decentral perfusion through artificial micro-capillaries. While the static medium exchange in petri dishes leads to metabolite peaks, perfusion culture pro作者: 真繁榮 時間: 2025-3-29 15:18 作者: 離開可分裂 時間: 2025-3-29 19:11 作者: 不能平靜 時間: 2025-3-29 23:42
Nature of Evil and Spiritual Intelligence,y, hiPSCs are expanded in two-dimensional static culture as colonies in the presence or absence of feeder cells. However, this expansion procedure is associated with lack of reproducibility and low cell yields. To fulfill the large cell number demand for clinical use, robust large-scale production o作者: Eviction 時間: 2025-3-30 01:03
Gabriel Flynn,Patricia H. Werhanel studies and have a huge potential in many therapeutic areas. ESCs can aggregate to form embryoid body (EB) in static suspension culture by spontaneous differentiation, which resembles an intact embryo; while static suspension culture cannot prevent agglomeration of cells and offers little control 作者: 膽汁 時間: 2025-3-30 06:15
People in Business: Context and Characterbilities, and secretions of trophic factors. In clinical application, a large quantity of therapeutically competent hMSCs is required that cannot be produced in conventional petri dish culture. Bioreactors are scalable and have the capacity to meet the production demand. Microcarrier suspension cult作者: Console 時間: 2025-3-30 08:42 作者: NAV 時間: 2025-3-30 14:26 作者: lattice 時間: 2025-3-30 19:34
Gabriel Flynn,Patricia H. Werhane of cells. However, there are only few germ line stem cells in the testis, contained in the fraction of undifferentiated spermatogonia. The lack of specific markers makes it difficult to isolate these cells. The long term maintenance and proliferation of nonrodent germ cells in culture has so far be作者: In-Situ 時間: 2025-3-30 23:39 作者: chassis 時間: 2025-3-31 02:02 作者: 樹膠 時間: 2025-3-31 06:14 作者: Accommodation 時間: 2025-3-31 12:26 作者: 大吃大喝 時間: 2025-3-31 13:52
Leadership Development Strategies,tions, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactor-based methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo c作者: prick-test 時間: 2025-3-31 18:28
