標(biāo)題: Titlebook: Biophysics of Membrane Proteins; Methods and Protocol Vincent L. G. Postis,Adrian Goldman Book 2020 Springer Science+Business Media, LLC, p [打印本頁] 作者: Aggrief 時間: 2025-3-21 19:45
書目名稱Biophysics of Membrane Proteins影響因子(影響力)
書目名稱Biophysics of Membrane Proteins影響因子(影響力)學(xué)科排名
書目名稱Biophysics of Membrane Proteins網(wǎng)絡(luò)公開度
書目名稱Biophysics of Membrane Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Biophysics of Membrane Proteins被引頻次
書目名稱Biophysics of Membrane Proteins被引頻次學(xué)科排名
書目名稱Biophysics of Membrane Proteins年度引用
書目名稱Biophysics of Membrane Proteins年度引用學(xué)科排名
書目名稱Biophysics of Membrane Proteins讀者反饋
書目名稱Biophysics of Membrane Proteins讀者反饋學(xué)科排名
作者: 決定性 時間: 2025-3-21 20:45
Thermostability Assays: a Generic and Versatile Tool for Studying the Functional and Structural Prop by changes in specific amino acid interactions. Finally, the assay can be used to study state-dependent domain interactions, for example, as part of regulatory mechanisms. The CPM thermostability assay represents a broadly applicable and versatile tool for a wide range of applications in the functi作者: 激怒 時間: 2025-3-22 03:03
Direct Monitoring of GPCR Reconstitution and Ligand-Binding Activity by Plasmon Waveguide Resonancens can also be investigated from cell membrane fragments overexpressing the receptor of interest, bypassing isolation and reconstitution procedures. The method is direct, sensitive, and does not rely on the use of any labeled material.作者: 是他笨 時間: 2025-3-22 06:48
Interpreting SAXS /WAXS Data with Explicit-Solvent Simulations: A Practical Guide trajectory as required to validate an MD simulation against experimental SWAXS data. Second, we present the structure refinement of an atomic model against SWAXS data using SAXS-driven MD simulations. Common technical problems together with appropriate solutions are discussed.作者: 偽證 時間: 2025-3-22 11:43 作者: 隨意 時間: 2025-3-22 13:11 作者: 鑲嵌細(xì)工 時間: 2025-3-22 21:00 作者: 大溝 時間: 2025-3-23 01:00 作者: 津貼 時間: 2025-3-23 04:54
https://doi.org/10.1007/978-1-4614-0359-3 trajectory as required to validate an MD simulation against experimental SWAXS data. Second, we present the structure refinement of an atomic model against SWAXS data using SAXS-driven MD simulations. Common technical problems together with appropriate solutions are discussed.作者: Notify 時間: 2025-3-23 09:31 作者: 帳單 時間: 2025-3-23 11:28
1064-3745 es to help further the understanding of key membrane protein structure and function for?students, academics, and industrial researchers..978-1-0716-0726-8978-1-0716-0724-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 極力證明 時間: 2025-3-23 16:32
Book 2020 and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary mate作者: 填料 時間: 2025-3-23 21:02
https://doi.org/10.1007/978-1-349-01728-7ers are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.作者: bypass 時間: 2025-3-24 00:56
Latin America, Media, and Revolutionecule: establishing path independence of this reaction is essential. Here we describe the procedures required to measure and verify path independence for the folding of outer membrane proteins in large unilamellar vesicles.作者: 帽子 時間: 2025-3-24 03:06
Latin America, Media, and Revolutionometry, and interactions with detergents/lipids/drugs. Furthermore, we discuss the sample requirements and experimental considerations unique to integral membrane protein native mass spectrometry research.作者: 妨礙 時間: 2025-3-24 08:36 作者: 詳細(xì)目錄 時間: 2025-3-24 12:47 作者: CRACY 時間: 2025-3-24 16:27
Solution X-Ray Scattering for Membrane Proteins, we describe a complete procedure to build a complete model of a transmembrane protein based on a truncated crystallographic model and experimental SEC-SAXS data using refractometry and UV absorption for internal validation of the measurements.作者: circumvent 時間: 2025-3-24 20:45
Short-Range Distance Measurement by Transition Metal Ion FRET metal such as nickel (Ni.), copper (Cu.), or cobalt (Co.). It can be applied to literally any protein where it is possible to perform site-specific incorporation of a fluorescent molecule. This chapter will explain the use and applications of tmFRET in detail using incorporation of the dye with cysteine chemistry on a purified protein sample.作者: 集合 時間: 2025-3-25 00:03
Quantifying the Interaction of Phosphite with ABC Transporters : MicroScale Thermophoresis and a Novers are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.作者: 藕床生厭倦 時間: 2025-3-25 06:03 作者: bifurcate 時間: 2025-3-25 08:34 作者: 可以任性 時間: 2025-3-25 11:54 作者: granite 時間: 2025-3-25 18:55
Latin America in the International Economyitution have been known for a?long?time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine for the obtention of monodisperse populations of proteoliposomes as well as for the quantitation of protein per liposome.作者: 使出神 時間: 2025-3-25 22:37
Latin America: A New Interpretatione protein. In a relatively short time (. 30?min), the molecular mass and quality of a membrane protein preparation can be determined. Here, I provide a detailed protocol on how to perform a SEC-MALLS run and show exemplary chromatograms and their analysis.作者: single 時間: 2025-3-26 00:18
Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomesitution have been known for a?long?time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine for the obtention of monodisperse populations of proteoliposomes as well as for the quantitation of protein per liposome.作者: ASSET 時間: 2025-3-26 04:26
Determination of the Molecular Mass of Membrane Proteins Using Size-Exclusion Chromatography with Mue protein. In a relatively short time (. 30?min), the molecular mass and quality of a membrane protein preparation can be determined. Here, I provide a detailed protocol on how to perform a SEC-MALLS run and show exemplary chromatograms and their analysis.作者: mediocrity 時間: 2025-3-26 09:29
Book 2020d cutting-edge, .Biophysics of Membrane Proteins: Methods and Protocols. aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for?students, academics, and industrial researchers..作者: Formidable 時間: 2025-3-26 13:08 作者: 繁重 時間: 2025-3-26 18:27 作者: gruelling 時間: 2025-3-27 00:34
Membrane Protein Production and Purification from , and , Insect Cellspecialized expensive equipment. Especially for insect cells, GFP fusions are particularly useful for localization and in-gel fluorescence detection of the proteins on SDS-PAGE. We give detailed protocols that can be used to screen the best expression and purification conditions for membrane protein study.作者: 確定無疑 時間: 2025-3-27 04:20
Examining Membrane Proteins by Neutron Scatteringackground, and a succinct description of the principles of analysis, of the instrumental and sample requirement, and of the practical steps, prior to the experiments, during the experiments and for data analysis.作者: hazard 時間: 2025-3-27 06:08 作者: 果核 時間: 2025-3-27 10:26
978-1-0716-0726-8Springer Science+Business Media, LLC, part of Springer Nature 2020作者: OVER 時間: 2025-3-27 15:10 作者: resuscitation 時間: 2025-3-27 21:41 作者: Culmination 時間: 2025-3-28 00:07
Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule F?rster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10.–10.?s).作者: 光亮 時間: 2025-3-28 03:45
https://doi.org/10.1007/978-1-349-01728-7 and structural studies. To overexpress the target membrane protein heterologously, especially an eukaryotic protein, a key step is to find the optimal host expression system and perform subsequent expression optimization. In this chapter, we describe protocols for screening membrane protein product作者: 刺耳 時間: 2025-3-28 09:15
https://doi.org/10.1007/978-1-349-01728-7f the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determination of the binding affinity of phosphite to three ABC transporter periplasmic-binding proteins from environmental microorganisms. ABC transport作者: 遭遇 時間: 2025-3-28 13:55 作者: notice 時間: 2025-3-28 15:40
The Olive Branch and the Arrows, protocol to the Na.-coupled sugar transporter SGLT1, the organic cation transporter OCT2, the Na./Ca. exchanger NCX, and the neuronal glutamate transporter EAAT3..The assay was developed for the commercially available SURFE.R N1 instrument (Nanion Technologies GmbH) which applies solid supported me作者: Critical 時間: 2025-3-28 22:18 作者: Fibrinogen 時間: 2025-3-28 23:38 作者: Spina-Bifida 時間: 2025-3-29 07:08 作者: Grandstand 時間: 2025-3-29 07:16
https://doi.org/10.1007/978-94-011-4870-2odels, either due to truncations introduced by the scientists or locally weak experimental data. This problem is even more common for transmembrane proteins, owing to the difficulties inherent in their crystallization. By the virtue of operating in solution, SAXS bypasses the problems with crystalli作者: DIS 時間: 2025-3-29 13:50
https://doi.org/10.1007/978-1-4614-0359-3tion. However, the interpretation of SWAXS data remains challenging owing to the low information content of the data and scattering contributions from the solvent. In recent years, methods for the interpretation of SWAXS data based on explicit-solvent molecular dynamics (MD) simulations have become 作者: Patrimony 時間: 2025-3-29 16:07 作者: Condense 時間: 2025-3-29 19:58 作者: 仔細(xì)檢查 時間: 2025-3-29 23:55 作者: 性冷淡 時間: 2025-3-30 07:26
The Making of the Twentieth Century is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule F?rster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10.–10.?s).作者: 引水渠 時間: 2025-3-30 09:31
The Making of the Twentieth Centurying the mechanisms behind protein function. Herein we describe a fluorescence-based method that enables the measurement of distances between specific domains within a protein and how it might change during protein function. The method is transition metal ion F?rster resonance energy transfer (tmFRET作者: 小官 時間: 2025-3-30 12:44 作者: expound 時間: 2025-3-30 19:02
Vincent L. G. Postis,Adrian GoldmanIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 迎合 時間: 2025-3-30 23:19
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/188370.jpg作者: Fracture 時間: 2025-3-31 02:51 作者: 罐里有戒指 時間: 2025-3-31 06:56
Quantifying the Interaction of Phosphite with ABC Transporters : MicroScale Thermophoresis and a Novf the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determination of the binding affinity of phosphite to three ABC transporter periplasmic-binding proteins from environmental microorganisms. ABC transport作者: mediocrity 時間: 2025-3-31 12:45
Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomesbic environment and allows?one to tackle their study from both functional and structural points of view. Methods for their rapid and efficient reconstitution have been known for a?long?time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine 作者: MEAN 時間: 2025-3-31 16:01
Functional Characterization of SLC Transporters Using Solid Supported Membranes protocol to the Na.-coupled sugar transporter SGLT1, the organic cation transporter OCT2, the Na./Ca. exchanger NCX, and the neuronal glutamate transporter EAAT3..The assay was developed for the commercially available SURFE.R N1 instrument (Nanion Technologies GmbH) which applies solid supported me作者: BRIDE 時間: 2025-3-31 18:59
Thermostability Assays: a Generic and Versatile Tool for Studying the Functional and Structural Propa thiol-reactive fluorochrome N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM) can be used. An unfolding profile is obtained when the fluorochrome becomes fluorescent on reaction with cysteine residues that have been exposed during thermal denaturation of the protein population. The
