標(biāo)題: Titlebook: Biomembrane Protocols; I. Isolation and An John M. Graham,Joan A. Higgins Book 1993 Humana Press 1993 Biomembran.Peroxisom.Western Blottin [打印本頁(yè)] 作者: 僵局 時(shí)間: 2025-3-21 18:43
書(shū)目名稱(chēng)Biomembrane Protocols影響因子(影響力)
作者: 心痛 時(shí)間: 2025-3-21 23:22 作者: 相容 時(shí)間: 2025-3-22 03:48 作者: considerable 時(shí)間: 2025-3-22 06:38
Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver,: basolateral and apical. In hepatocytes, the basolateral domain includes the sinusoidal front, which faces the space of Disse and is involved in exchange of metabolites with the circulation, and the lateral surface, which is contiguous to neighboring hepatocytes. The apical or bile canalicular doma作者: 多骨 時(shí)間: 2025-3-22 10:35 作者: artless 時(shí)間: 2025-3-22 15:38
The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components,ort of newly synthesized lysosomal enzymes from the trans-Golgi network, and at the plasma membrane they are responsible for receptor-mediated endocytosis of selected ligands (.). Coated vesicles are the best characterized of all transport organelles, owing in large measure to the ease with which th作者: Truculent 時(shí)間: 2025-3-22 17:49
Isolation of Membranes from Tissue Culture Cells,ticularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue cultur作者: uncertain 時(shí)間: 2025-3-22 22:37 作者: 龍卷風(fēng) 時(shí)間: 2025-3-23 05:17
Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protron flow and phosphorylation, metabolite transport, and protein targeting. Whatever the subsequent use of the chloroplasts, it is preferable to attempt to isolate . chloroplasts, as the organelle envelope will protect the stroma and thylakoids from the deleterious effects of degradative enzymes and作者: 巨碩 時(shí)間: 2025-3-23 08:27
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells,onsiderable expertise. Although it is relatively easy to prepare a crude mitochondrial fraction by differential centrifugation, marked changes in functional capabilities and morphological appearance are often observed in such fractions, suggesting that some degree of structural damage has occurred d作者: 咆哮 時(shí)間: 2025-3-23 10:20
Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption,s (e.g., size and density). In contrast, immunological techniques rely on biological differences, i.e., the expression of different antigens. The most popular immunological method for subcellular fractionation is immunoadsorption onto a solid phase, which offers the prospect of fast purification in 作者: 緯線(xiàn) 時(shí)間: 2025-3-23 16:35 作者: 寬敞 時(shí)間: 2025-3-23 18:58 作者: Immunotherapy 時(shí)間: 2025-3-24 00:18 作者: 小木槌 時(shí)間: 2025-3-24 06:19
Analysis of Fatty Acids by Gas Liquid Chromatography,ngle carboxyl group. FA can be classified as saturated, monounsaturated, or polyunsaturated (.). Saturates have all single carbon-to-carbon bonds. Monounsaturates contain a single . double bond, whereas polyunsaturates have two or more. A list of the commonly occurring natural FA in animal tissues i作者: Inoperable 時(shí)間: 2025-3-24 08:07 作者: reptile 時(shí)間: 2025-3-24 13:33
https://doi.org/10.1385/0896032361Biomembran; Peroxisom; Western Blotting; biology; biomembrane; enzymes; glycoprotein; immunology; membrane; m作者: 自負(fù)的人 時(shí)間: 2025-3-24 16:47
https://doi.org/10.1007/978-3-0346-0439-0anes. In many cases, a study of a particular membrane presupposes that it can be isolated by disruption of the cells and fractionation of the resulting homogenate, and then identified unambiguously. The early chapters of this volume, ., are concerned with this fractionation process. Later chapters a作者: Mendacious 時(shí)間: 2025-3-24 19:13 作者: PALL 時(shí)間: 2025-3-25 02:12
https://doi.org/10.1007/978-3-658-18838-2are the tubular membranes of the smooth and rough endoplasmic reticulum. Almost without exception, these vesicles retain their normal orientation, i.e., the cytoplasmic face is outermost. This is particularly clear in the case of the rough endoplasmic reticulum, for the ribosomes remain attached on 作者: Lipoprotein 時(shí)間: 2025-3-25 07:07 作者: intimate 時(shí)間: 2025-3-25 07:43
Cormac Walsh,Gisela Kangler,Markus Schaffertem through the necessary metabolic processes. Studies of the sequential steps of these events require methods of following the fate of the molecule from its internalization to its degradation in lysosomes or its transfer to other intracellular compartments.作者: 無(wú)聊點(diǎn)好 時(shí)間: 2025-3-25 12:20 作者: 魯莽 時(shí)間: 2025-3-25 16:37
Cormac Walsh,Gisela Kangler,Markus Schaffertticularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue cultur作者: geriatrician 時(shí)間: 2025-3-25 20:44 作者: charisma 時(shí)間: 2025-3-26 02:00 作者: Lipohypertrophy 時(shí)間: 2025-3-26 06:42
Ressourcen Was der Mensch draus macht,onsiderable expertise. Although it is relatively easy to prepare a crude mitochondrial fraction by differential centrifugation, marked changes in functional capabilities and morphological appearance are often observed in such fractions, suggesting that some degree of structural damage has occurred d作者: 六邊形 時(shí)間: 2025-3-26 11:11
Kraft des Eises Gletscher und Inlandeis,s (e.g., size and density). In contrast, immunological techniques rely on biological differences, i.e., the expression of different antigens. The most popular immunological method for subcellular fractionation is immunoadsorption onto a solid phase, which offers the prospect of fast purification in 作者: Minuet 時(shí)間: 2025-3-26 12:59 作者: 鳥(niǎo)籠 時(shí)間: 2025-3-26 18:00 作者: 開(kāi)玩笑 時(shí)間: 2025-3-27 00:20
Partner im All Die Erde im Sonnensystem,owing extraction from tissues or membranes. Before determination, cholesterol esters must be hydrolyzed using either chemical methods or cholesterol ester hydrolase (EC 3.11.13) (..). Enzymic methods for cholesterol determination are more sensitive and convenient and are generally used in preference作者: anarchist 時(shí)間: 2025-3-27 02:09 作者: 肌肉 時(shí)間: 2025-3-27 08:19 作者: STYX 時(shí)間: 2025-3-27 11:42
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/188145.jpg作者: 兇兆 時(shí)間: 2025-3-27 15:27
Zum Nachdenken über LandschaftsarchitekturThis fraction is defined broadly as the material that will sediment at about 3500. for 10 min from a postnuclear supernatant. It contains the nuclei that failed to sediment at 1000. for 5–10 min, the largest mitochondria, and very few other organelles, such as lysosomes and peroxisomes.作者: Cougar 時(shí)間: 2025-3-27 20:48
https://doi.org/10.1007/978-3-658-18838-2Continuous-flow electrophoresis (CFE), which separates particles on the basis of surface charge density, should be regarded as an adjunct to centrifugation rather than as an alternative for membrane fractionation. The equipment is expensive and has been used for relatively specialized tasks, not for the routine isolations from a total homogenate.作者: 眉毛 時(shí)間: 2025-3-27 23:24
Aus dem Inneren Vulkanismus und Erdbeben,The choice of a protein assay for membranes and membrane proteins is dependent on a number of considerations: sensitivity, specificity (both with respect to variation between proteins and interference by nonprotein components), and simplicity.作者: 和平 時(shí)間: 2025-3-28 03:31
Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes froThis fraction is defined broadly as the material that will sediment at about 3500. for 10 min from a postnuclear supernatant. It contains the nuclei that failed to sediment at 1000. for 5–10 min, the largest mitochondria, and very few other organelles, such as lysosomes and peroxisomes.作者: 向前變橢圓 時(shí)間: 2025-3-28 06:52
Continuous-Flow Electrophoresis,Continuous-flow electrophoresis (CFE), which separates particles on the basis of surface charge density, should be regarded as an adjunct to centrifugation rather than as an alternative for membrane fractionation. The equipment is expensive and has been used for relatively specialized tasks, not for the routine isolations from a total homogenate.作者: 下級(jí) 時(shí)間: 2025-3-28 13:01 作者: 歡樂(lè)中國(guó) 時(shí)間: 2025-3-28 16:11
Biomembrane Protocols978-1-59259-506-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Obligatory 時(shí)間: 2025-3-28 21:17
1064-3745 Overview: 978-1-59259-506-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Limpid 時(shí)間: 2025-3-29 00:49 作者: 排名真古怪 時(shí)間: 2025-3-29 05:43
Gisela Kangler,Markus Schaffert,Cormac Walshort of newly synthesized lysosomal enzymes from the trans-Golgi network, and at the plasma membrane they are responsible for receptor-mediated endocytosis of selected ligands (.). Coated vesicles are the best characterized of all transport organelles, owing in large measure to the ease with which they can be purified.作者: 牛的細(xì)微差別 時(shí)間: 2025-3-29 07:27
Cormac Walsh,Gisela Kangler,Markus Schaffertticularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue culture cells, . Graham (.).作者: Handedness 時(shí)間: 2025-3-29 14:49 作者: bourgeois 時(shí)間: 2025-3-29 17:56 作者: helper-T-cells 時(shí)間: 2025-3-29 23:20
The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components,ort of newly synthesized lysosomal enzymes from the trans-Golgi network, and at the plasma membrane they are responsible for receptor-mediated endocytosis of selected ligands (.). Coated vesicles are the best characterized of all transport organelles, owing in large measure to the ease with which they can be purified.作者: Odyssey 時(shí)間: 2025-3-29 23:56
Isolation of Membranes from Tissue Culture Cells,ticularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue culture cells, . Graham (.).作者: 原始 時(shí)間: 2025-3-30 04:51
Measurement of Cholesterol in Membranes,owing extraction from tissues or membranes. Before determination, cholesterol esters must be hydrolyzed using either chemical methods or cholesterol ester hydrolase (EC 3.11.13) (..). Enzymic methods for cholesterol determination are more sensitive and convenient and are generally used in preference to chemical methods.作者: MINT 時(shí)間: 2025-3-30 11:03
Landschaftsarchitektur visualisieren the rate of sedimentation of the nuclei, which is the reason for the use of high relative centrifugal forces (rcf) (normally 100,000.). Furthermore, high viscosity sucrose solutions are difficult to prepare and measure out accurately.作者: 易于交談 時(shí)間: 2025-3-30 15:09 作者: Thyroxine 時(shí)間: 2025-3-30 17:37
The Isolation of Nuclei and Nuclear Membranes from Rat Liver, the rate of sedimentation of the nuclei, which is the reason for the use of high relative centrifugal forces (rcf) (normally 100,000.). Furthermore, high viscosity sucrose solutions are difficult to prepare and measure out accurately.作者: 障礙物 時(shí)間: 2025-3-30 20:53 作者: Genteel 時(shí)間: 2025-3-31 03:18 作者: 鳥(niǎo)籠 時(shí)間: 2025-3-31 06:33 作者: Foreknowledge 時(shí)間: 2025-3-31 09:55 作者: considerable 時(shí)間: 2025-3-31 14:18 作者: 魔鬼在游行 時(shí)間: 2025-3-31 18:35
Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells,patocytes and the basolateral membranes of enterocytes both tend to form vesicles, whereas hepatocyte contiguous membranes and enterocyte microvillar membranes will remain intact, as long as the homogenization is relatively mild (..).作者: Aggrandize 時(shí)間: 2025-3-31 21:52
The Isolation of Membranes from Bacteria,ing membrane rigidity. Although variable, the major lipids are phosphatidylglycerol and phosphatidylethanolamine in gram-positive bacteria, and phosphatidylethanolamine with smaller amounts of phosphatidylglycerol in gram-negative bacteria.作者: Eructation 時(shí)間: 2025-4-1 02:22 作者: 上下連貫 時(shí)間: 2025-4-1 07:26 作者: archaeology 時(shí)間: 2025-4-1 10:51 作者: 無(wú)能性 時(shí)間: 2025-4-1 17:54 作者: 時(shí)代錯(cuò)誤 時(shí)間: 2025-4-1 18:34
https://doi.org/10.1007/978-3-0346-0439-0g homogenate, and then identified unambiguously. The early chapters of this volume, ., are concerned with this fractionation process. Later chapters are concerned with the determination of composition, whereas in the second volume, ., the methods associated with architecture and function predominate.作者: 鬼魂 時(shí)間: 2025-4-2 00:34 作者: ZEST 時(shí)間: 2025-4-2 06:40 作者: 品嘗你的人 時(shí)間: 2025-4-2 10:15 作者: 出來(lái) 時(shí)間: 2025-4-2 11:48
Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver,in is specialized for the transport of bile acids and is separated from the basolateral domain by tight junctions, which prevent lateral diffusion of membrane proteins and outer leaflet lipids. Biochemical analysis of hepatocyte PM constituents requires the isolation of substantial amounts of this organelle.作者: Fermentation 時(shí)間: 2025-4-2 18:55
Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption,high yield under conditions that do not expose organelles to unusual media or to osmotic shock. Immunoisolation techniques also have the advantage of being applicable to situations where only a small amount of cell material is available, e.g., tissue culture cells.
