標(biāo)題: Titlebook: Biomembrane Protocols; II. Architecture and John M. Graham,Joan A. Higgins Book 1994 Springer Science+Business Media New York 1994 [打印本頁] 作者: 悲傷我 時間: 2025-3-21 19:44
書目名稱Biomembrane Protocols影響因子(影響力)
作者: Enliven 時間: 2025-3-22 00:16 作者: Measured 時間: 2025-3-22 03:54
Gerd Lutze,Alfred Schultz,Karin Luzi detergent to be removed following purification. Ionic detergents, such as cholate, usually fall into this category, although the nonionic detergent octylglucoside also has a high critical micelle concentration. Nonionic detergents, such as Triton X-100 and C.E., cannot be removed by conventional di作者: 躺下殘殺 時間: 2025-3-22 08:38 作者: ADAGE 時間: 2025-3-22 10:01 作者: 逃避系列單詞 時間: 2025-3-22 15:01 作者: 北極人 時間: 2025-3-22 18:39
Crystallization of Membrane Proteins for X-Ray Analysis,tudy of bacteriorhodopsin has the resolution of the structural analysis approached that obtainable by X-ray crystallography (.). The formation of two-dimensional crystalline arrays will not be considered in this chapter. The first membrane protein crystal structure, the bacterial photosynthetic reac作者: LEERY 時間: 2025-3-23 00:48 作者: Interstellar 時間: 2025-3-23 03:33 作者: 輕快帶來危險 時間: 2025-3-23 08:15
Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spe been presented (.,.). From such data, straight-line plots of the mobile: immobile lipid ratio against lipid:protein ratio are usually constructed, with the intercept on the lipid:protein ratio axis being taken to be the number of lipid-binding sites on the protein and the slope giving the relative 作者: 青春期 時間: 2025-3-23 10:26 作者: covert 時間: 2025-3-23 16:10 作者: acrimony 時間: 2025-3-23 21:23 作者: 烤架 時間: 2025-3-24 02:12
Olaf Kühne,Karsten Berr,Petra Lohmannwealth of experience accumulated in the course of the crystallization and structural analyses of several hundred soluble globular proteins and their complexes, the process of crystallization still remains something of an art, and is often the rate limiting step of any analysis. For membrane proteins作者: Herd-Immunity 時間: 2025-3-24 04:41
Olaf Kühne,Karsten Berr,Petra Lohmannpartmentalization of function is established by the maintenance of asymmetric structure and function between the apical and basolateral surfaces of the cell. This asymmetry (polarity) extends on a biochemical level to the membrane proteins of the apical and basolateral plasma membrane. Indeed, many 作者: NUL 時間: 2025-3-24 09:47 作者: 神圣將軍 時間: 2025-3-24 11:01 作者: Hyaluronic-Acid 時間: 2025-3-24 18:36
Karl-Otto Wenkel,Alfred Schultz,Gerd Lutzes with a myeloma cell line; selection, cloning, and freezing of hybridoma cell lines; and screening for monoclonal antibody production. Part A of this chapter will be concerned with the production of hybridoma cell lines, and Part B with the screening procedures.作者: craven 時間: 2025-3-24 21:17
Gerd Lutze,Alfred Schultz,Karin Luzition of membrane proteins has been achieved without the use of detergents (e.g., by sonication of membrane proteins with lipids), detergent-based methods are more widely used and appear to be more reproducible (.). If a particular protein is to be purified in an active form, then particular attentio作者: 鉤針織物 時間: 2025-3-24 23:42 作者: 2否定 時間: 2025-3-25 06:44 作者: BIBLE 時間: 2025-3-25 08:16 作者: grudging 時間: 2025-3-25 15:01
Immerfeuchte und Sommerfeuchte Tropen one-for-one exchange in vitro of phospholipid molecules between membranes, lipoproteins, or liposomes. The functions of PL-TP in vivo have not been established; however, it is likely that they are involved in the transfer of phospholipid between organelle membranes during membrane biogenesis and me作者: Manifest 時間: 2025-3-25 16:24
Immerfeuchte und Sommerfeuchte Tropenipids nor membrane proteins are distributed symmetrically over inner and outer aspects of cellular membranes, and knowledge of the topological distribution is a prerequisite for understanding the functional properties of membranes. This chapter will deal with a recently developed, noninvasive techni作者: SMART 時間: 2025-3-25 23:57 作者: 格子架 時間: 2025-3-26 00:42
,Streifzüge in der Kulturlandschaft,s sonicated an aqueous suspension of egg lecithin mixed with a small percentage of a spin-labeled phosphatidylcholine. The spin-labeled lipid had a nitroxide group on its polar head group that was directly exposed to the aqueous phase. Sodium ascorbate at 0°C, a nonpermeant reducing agent, very rapi作者: 有罪 時間: 2025-3-26 04:58
Ebenen landschaftlicher Resilienz,properties of cell membranes (.,.). The ability of protein molecules embedded in the plasma membrane to move laterally from one location to another, for example, in lymphocyte capping, depends on the presence of a low-viscosity (fluid) lipid domain in the membrane. Traffic of membrane materials to a作者: 易彎曲 時間: 2025-3-26 09:27
https://doi.org/10.1007/978-3-662-61029-9 physiological phenomena. Over the last 30 years, a number of techniques have been employed to assess changes in the amounts of these two molecules. Many, however, suffered from lack of sensitivity and/or ease of performance. Since the early 1970s, two methods have been developed that to a large ext作者: HILAR 時間: 2025-3-26 16:37
Die Baukunst der Landschaftsmalerei, that transduce as diverse a range of biological functions as protein synthesis, transmembrane signaling, intracellular trafficking, and cell proliferation (reviewed in .). The application of biochemical and molecular biological techniques has substantially increased our understanding of the structu作者: 小歌劇 時間: 2025-3-26 19:59 作者: transient-pain 時間: 2025-3-26 22:34
https://doi.org/10.1007/978-3-0346-0439-0des, play a central role in cell signal transduction. Activation of a hormone-sensitive phospholipase C (phosphoinositidase C) results in the rapid catabolism of the polyphosphoinositides to form the two second messengers inositol 1,4,5-trisphosphate (Ins(l,4,5)P.), a water soluble phosphoinositol t作者: endure 時間: 2025-3-27 02:55
Landschaftsarchitektur visualisierencribed, together with simple separation procedures to resolve them into their general classes (InsP.; InsP., and so on, and PtdIns, PtdInsP, and PtdInsP.). However the metabolism of phosphoinositols is complex (.,.), leading to the formation of several isomeric forms in each class (.. of . and .).作者: Canary 時間: 2025-3-27 09:15 作者: BUCK 時間: 2025-3-27 13:04 作者: 惰性女人 時間: 2025-3-27 13:47
Biomembrane Protocols978-1-59259-514-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 修正案 時間: 2025-3-27 20:23 作者: GUISE 時間: 2025-3-28 01:03 作者: 凝視 時間: 2025-3-28 02:06 作者: Spinal-Fusion 時間: 2025-3-28 08:06 作者: 無畏 時間: 2025-3-28 12:34
Olaf Kühne,Karsten Berr,Petra Lohmanne cell. This asymmetry (polarity) extends on a biochemical level to the membrane proteins of the apical and basolateral plasma membrane. Indeed, many of the polarized functions of epithelia are mediated by membrane proteins enriched or restricted to one particular cellular domain (.,.).作者: 殺死 時間: 2025-3-28 15:29
https://doi.org/10.1007/978-3-642-55426-1ble in permeable vesicles or only accessible in inside-out vesicles are considered to be in the inner leaflet of the membrane bilayer. However, the characteristics of the membrane preparation under investigation and the enzyme probe are critical in these experiments. The following criteria are important.作者: CHANT 時間: 2025-3-28 22:47 作者: Pericarditis 時間: 2025-3-29 02:12 作者: Commentary 時間: 2025-3-29 06:13 作者: 紡織品 時間: 2025-3-29 07:50 作者: Demonstrate 時間: 2025-3-29 11:28
Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination,e cell. This asymmetry (polarity) extends on a biochemical level to the membrane proteins of the apical and basolateral plasma membrane. Indeed, many of the polarized functions of epithelia are mediated by membrane proteins enriched or restricted to one particular cellular domain (.,.).作者: pulse-pressure 時間: 2025-3-29 16:24
Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as ble in permeable vesicles or only accessible in inside-out vesicles are considered to be in the inner leaflet of the membrane bilayer. However, the characteristics of the membrane preparation under investigation and the enzyme probe are critical in these experiments. The following criteria are important.作者: 立即 時間: 2025-3-29 20:51
Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry,ution is a prerequisite for understanding the functional properties of membranes. This chapter will deal with a recently developed, noninvasive technique to assess the distribution of phospholipids over the two leaflets of biological membranes with emphasis on the distribution of negatively charged phospholipids (.,.).作者: 性別 時間: 2025-3-30 00:56 作者: 尊重 時間: 2025-3-30 04:18 作者: 磨碎 時間: 2025-3-30 12:12
Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Protabolism of the polyphosphoinositides to form the two second messengers inositol 1,4,5-trisphosphate (Ins(l,4,5)P.), a water soluble phosphoinositol that promotes the release of Ca. from intracellular stores, and diacylglycerol (DG), which remains in the plasma membrane and activates protein kinase C (.–.). .. for a summary of the pathways.作者: QUAIL 時間: 2025-3-30 12:52
Landschaft, Identit?t und Gesundheitopology in the membrane, and their isolation, using antipeptide antibodies, since antibodies raised against short peptides (10–20 amino acid residues) frequently recognize the corresponding sequence in intact proteins (.).作者: 食草 時間: 2025-3-30 20:14
https://doi.org/10.1007/978-3-662-61029-9 that, with minor modifications, can be used to detect levels of cyclic nucleotides down to 1 fmol/assay tube; this is discussed in detail below (.,.). The method is equally applicable to the measurement of all cyclic nucleotides; however, for clarity only that for cAMP is described.作者: 柔聲地說 時間: 2025-3-30 20:50 作者: 自傳 時間: 2025-3-31 03:00 作者: 出汗 時間: 2025-3-31 05:33 作者: Polydipsia 時間: 2025-3-31 09:42
Ebenen landschaftlicher Resilienz,nd from the cell surface depends on the ability of membrane vesicles to fuse with and to bud from the plasma membrane. Such processes are essential for the release of neurotransmitters into the synaptic cleft and play a role in a variety of diseases involving infection of cells by enveloped viruses, such as influenza virus (.).作者: 角斗士 時間: 2025-3-31 16:21
Book 1994 has been driven by the development of new techniques for studying membrane components and their interactions. Tradition- ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have作者: Magnitude 時間: 2025-3-31 21:19
Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Prmbrane remodeling. Since PL-TP only exchange the phospholipid of the outer leaflet of membrane bilayers and do not appear to alter the structure of membranes, they provide a unique tool for investigating the transverse distribution of membrane phospholipids.作者: Lethargic 時間: 2025-3-31 23:59 作者: hypnotic 時間: 2025-4-1 03:19 作者: 觀點 時間: 2025-4-1 07:23 作者: inchoate 時間: 2025-4-1 14:06
Biochemical Methods to Determine Cell-Surface Topography,c activity of GAO that oxidizes these residues at the carbon-6 position. The method is generally applicable and may be used in conjunction with methods described in Part B of this chapter and in .. .., Part D in this volume.
