作者: 防水 時間: 2025-3-21 23:37 作者: 不理會 時間: 2025-3-22 02:36 作者: Ischemic-Stroke 時間: 2025-3-22 05:43 作者: Ceramic 時間: 2025-3-22 12:38 作者: Generosity 時間: 2025-3-22 14:03
Microfluidic Device Technologies for Digestion, Disaggregation, and Filtration of Tissue Samples foronal characteristics, or utilized for therapeutic purposes. A major driver is the development of single cell analysis methods, which are best poised to assess cellular heterogeneity and discover rare cells. Current tissue dissociation methods are inefficient, produce variable results, and require ma作者: 基因組 時間: 2025-3-22 20:02 作者: BRIBE 時間: 2025-3-22 21:12 作者: 清楚 時間: 2025-3-23 02:02
Fluorescence Lifetime Imaging Probes for Cell-Based Measurements of Enzyme Activityino acids, often on side chain functional groups, to change the physiochemical landscape of the protein and thus its biophysical behavior. In particular, protein kinases are enzymes that transfer phosphate from ATP to serine, threonine, or tyrosine in protein substrates. They are key regulators of v作者: 施加 時間: 2025-3-23 06:17 作者: inventory 時間: 2025-3-23 11:55
Node-Pore Sensing for Characterizing Cells and Extracellular VesiclesNPS involves measuring the modulated current pulse caused by a cell transiting a microfluidic channel that has been segmented by a series of inserted nodes. As the current pulse reflects the number of nodes and segments of the channel, NPS can achieve exquisite sensitivity. Thus, when used as a Coul作者: guardianship 時間: 2025-3-23 14:38
Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobescific signature wherein a variety of proteins and nucleic acids are selectively packaged. Growing evidence has shown important biological roles and clinical relevance of EVs in diseases. For EV-related studies to thrive, rapid efficient isolation of pure EVs is a prerequisite. However, lengthy proce作者: 巡回 時間: 2025-3-23 20:26 作者: 使無效 時間: 2025-3-23 23:14 作者: HACK 時間: 2025-3-24 03:22 作者: ALT 時間: 2025-3-24 09:35 作者: 流逝 時間: 2025-3-24 13:29 作者: BANAL 時間: 2025-3-24 17:16 作者: configuration 時間: 2025-3-24 22:18 作者: 同時發(fā)生 時間: 2025-3-25 02:12
Introduction: Overview of the Volumenance energy transfer mechanism. We describe the use of a microwell chip to achieve single-cell resolution and demonstrate the procedure for on-chip immunostaining. Finally, we provide a method for data extraction, correction, and processing.作者: 嘲笑 時間: 2025-3-25 06:04 作者: 真繁榮 時間: 2025-3-25 09:53 作者: ascend 時間: 2025-3-25 12:28
Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensorsr GTP quantification in live cells. Therefore, in the current chapter, we describe the methodology for the quantitative assessment of spatiotemporal changes in GTP levels in the cells using genetically encoded fluorescent ratiometric GTP sensors termed GEVALs for .TP .uators.作者: 緩和 時間: 2025-3-25 17:54 作者: 拱形面包 時間: 2025-3-25 20:13
The Path of Traditional Labor Politics,c principles. In conjunction with this platform, we present a streamlined protocol for solid-phase DNA extraction from cells and bisulfite conversion of genomic DNA, minimizing the processing steps and reagent volume for implementation on a compact assay platform.作者: Eeg332 時間: 2025-3-26 00:25 作者: constellation 時間: 2025-3-26 07:42 作者: Instinctive 時間: 2025-3-26 12:25
Book 2022ary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols..?..Authoritative and practical,?.Biomedical Engineering Technologies, Volume 2.?provides technical details in descriptions of major technologies by experts in the field..作者: 開頭 時間: 2025-3-26 12:57
Jutta Allmendinger,Christian Gaggermeierter counter, NPS can measure the sub-micron size increase of antibody-coated colloids to which EVs are specifically bound. By simply inserting between two nodes a “contraction” channel through which cells can squeeze, one can mechanically phenotype cells. We discuss the details of performing these two NPS applications.作者: SOB 時間: 2025-3-26 18:39 作者: 尾巴 時間: 2025-3-26 23:56 作者: 翻布尋找 時間: 2025-3-27 03:11 作者: 高談闊論 時間: 2025-3-27 05:59
978-1-0716-1813-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: expire 時間: 2025-3-27 12:57 作者: 范圍廣 時間: 2025-3-27 17:31 作者: FUSE 時間: 2025-3-27 20:31 作者: Distribution 時間: 2025-3-27 22:47 作者: overrule 時間: 2025-3-28 02:41
Labor Markets in a Globalizing Worldind cancer cells that are clinically undetectable. Recent evidence has shown that subpopulations of these residual cancer cells enter into a prolonged dormant state, remaining quiescent for months to years, and eventually lead to metastases and relapse (Sosa et al. Nat Rev Cancer 14:611–622, 2014). 作者: Stagger 時間: 2025-3-28 08:15 作者: Statins 時間: 2025-3-28 14:06
Binh T. Nguyen,Loi Chi Cu,Chien Thang Nguyen microenvironment drives disease progression as well as therapeutic resistance. A number of challenges arise for researchers examining these cell-microenvironment interactions: (1) Tissue microenvironments are combinatorial and dynamic systems, and in pathological situations like cancer, microenviro作者: engender 時間: 2025-3-28 16:38
Introduction: Overview of the Volume These probes are encapsulated in liposomes and delivered into cells, where they continuously report on AKT signaling activities through a F?ster resonance energy transfer mechanism. We describe the use of a microwell chip to achieve single-cell resolution and demonstrate the procedure for on-chip i作者: AVOW 時間: 2025-3-28 21:05
The Widening and Deepening of Human Capital,onal characteristics, or utilized for therapeutic purposes. A major driver is the development of single cell analysis methods, which are best poised to assess cellular heterogeneity and discover rare cells. Current tissue dissociation methods are inefficient, produce variable results, and require ma作者: Indent 時間: 2025-3-29 01:24
Labor Markets, Migration, and Mobility precision oncology field where tumor genotyping is often essential for the indication of many advanced and targeted therapies. National Comprehensive Cancer Network (NCCN) guidelines now mandate molecular testing for clinically actionable targets in certain malignancies. Utilizing advanced stage lu作者: 無關緊要 時間: 2025-3-29 04:13 作者: CHOIR 時間: 2025-3-29 07:48 作者: Prognosis 時間: 2025-3-29 13:28
Gabriel Felbermayr,Wilhelm Kohlereration of several basal cellular phenotypes including cell motility, invasion, and tumorigenesis. However, until recently, no tools were available for GTP quantification in live cells. Therefore, in the current chapter, we describe the methodology for the quantitative assessment of spatiotemporal c作者: 藕床生厭倦 時間: 2025-3-29 19:09 作者: JOG 時間: 2025-3-29 20:54
Do Values Matter for Intra-EU Migration?cific signature wherein a variety of proteins and nucleic acids are selectively packaged. Growing evidence has shown important biological roles and clinical relevance of EVs in diseases. For EV-related studies to thrive, rapid efficient isolation of pure EVs is a prerequisite. However, lengthy proce作者: Disk199 時間: 2025-3-30 01:45 作者: 懶惰民族 時間: 2025-3-30 07:03 作者: canonical 時間: 2025-3-30 10:26
https://doi.org/10.1057/9780230106284ngle molecule sensitivity, can probe how membrane receptors are organized under both normal and pathological conditions. While a number of receptors have been extensively studied in cultured cells, technical challenges have largely impeded their robust quantification in tissue samples. To rigorously作者: cuticle 時間: 2025-3-30 16:27 作者: 啤酒 時間: 2025-3-30 18:18
Xiaochun Li,Ping He,Yu Zhou,Zheyu Dongthat are toxic and carcinogenic, such as chloroform and methyl-tert-butyl ether for lipidomics, or caustic chaotropic solutions for genomics and transcriptomics, such as guanidine or urea. A common preservation solution for pathology is formaldehyde, which is a carcinogen. Use of acetonitrile as a u作者: 鎮(zhèn)壓 時間: 2025-3-31 00:10
Xiaochun Li,Yuanting Xu,Dianshuang Wangamage and damage to the sugar-phosphate backbone of DNA. Double Strand Breaks (DSBs) are the most severe form of DNA damage and if left unchecked, may precipitate genomic rearrangements, cell death or contribute to malignancy. In clinical contexts, radiation is often used to induce DSBs as a form of作者: Ceramic 時間: 2025-3-31 02:52 作者: obviate 時間: 2025-3-31 06:04
Method to Isolate Dormant Cancer Cells from Heterogeneous Populationsgy 05:1–10, 2017; Reátegui et al. J Mater Chem B 2:7440–7448, 2014). Isolated DCCs can then be recovered from the gel and analyzed. Here we describe this immobilization method that can be used to isolate DCCs from heterogeneous cell populations that may also include dormancy-incapable cancer cells a作者: 遠足 時間: 2025-3-31 11:23
Label-Free Morphological Phenotyping of In Vitro 3D Microtumorsing MATLAB program. With this method, we have been able to show structural and spatial heterogeneity among single cells and surrounding regions in tumor spheroids, and between different cell types in tumor-fibroblast co-cultured spheroids. Our method has the potential to both bridge the gap between 作者: 安心地散步 時間: 2025-3-31 16:28 作者: 陪審團每個人 時間: 2025-3-31 20:09
Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision MedicineSynergy between microdissection and droplet digital PCR (ddPCR) enhances the molecular analysis. We demonstrate here a methodology that illustrates genotyping of a solid tumor from a small tissue biopsy sample in a time- and cost-efficient manner, using immunostain targeting as an example.作者: 違抗 時間: 2025-3-31 23:27 作者: 具體 時間: 2025-4-1 05:25 作者: 迅速成長 時間: 2025-4-1 09:41 作者: notification 時間: 2025-4-1 11:05
