標題: Titlebook: Biomarker Methods in Drug Discovery and Development; Feng Wang Book 2008 Humana Press 2008 Clinical sample handling.DNA.Drug efficacy and [打印本頁] 作者: Ford 時間: 2025-3-21 18:01
書目名稱Biomarker Methods in Drug Discovery and Development影響因子(影響力)
書目名稱Biomarker Methods in Drug Discovery and Development影響因子(影響力)學(xué)科排名
書目名稱Biomarker Methods in Drug Discovery and Development網(wǎng)絡(luò)公開度
書目名稱Biomarker Methods in Drug Discovery and Development網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Biomarker Methods in Drug Discovery and Development被引頻次
書目名稱Biomarker Methods in Drug Discovery and Development被引頻次學(xué)科排名
書目名稱Biomarker Methods in Drug Discovery and Development年度引用
書目名稱Biomarker Methods in Drug Discovery and Development年度引用學(xué)科排名
書目名稱Biomarker Methods in Drug Discovery and Development讀者反饋
書目名稱Biomarker Methods in Drug Discovery and Development讀者反饋學(xué)科排名
作者: Arresting 時間: 2025-3-21 21:30
Gene Expression-Based Biomarkers of Drug Safety, toxicity for use in preclinical and clinical studies. These biomarkers may have diagnostic and/or predictive values and may consist of single gene products or of gene sets or gene expression signatures. Derivation and validation of these molecular markers involves supervised classification methods 作者: Transfusion 時間: 2025-3-22 01:58 作者: 朋黨派系 時間: 2025-3-22 05:50
Real-Time Polymerase Chain Reaction Gene Expression Assays in Biomarker Discovery and Validation,creasingly important role in all phases of drug development as well as regulatory decision making. However, translating biomarker discovery into clinically useful tests in a time- and cost-effective manner remains a significant challenge. Real-time polymerase chain reaction (PCR) technology, in part作者: Aids209 時間: 2025-3-22 11:23 作者: bronchodilator 時間: 2025-3-22 14:49 作者: Blatant 時間: 2025-3-22 19:43
The Use of Two-Dimensional Gel Electrophoresis for Plasma Biomarker Discovery, rapidly growing arsenal of proteomics technologies. 2-DE combines two orthogonal and independent electrophoretic steps: isoelectric focusing (IEF) and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). At present, 2-DE is capable of simultaneously detecting and quantifying up to 作者: Ergots 時間: 2025-3-22 21:39 作者: antenna 時間: 2025-3-23 03:41 作者: COLON 時間: 2025-3-23 07:08 作者: homocysteine 時間: 2025-3-23 10:17 作者: 情節(jié)劇 時間: 2025-3-23 14:39
Sandwich ELISA Microarrays: ,,ltiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of one’s antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the step作者: Tartar 時間: 2025-3-23 21:19 作者: Vldl379 時間: 2025-3-24 02:04
GC-MS-Based Metabolomics,e 1980s. Only during the past few years has the technology been more widely used for metabolomics studies in animals and humans with the aim of toxicology and biomarker discovery, disease diagnosis and classification..GC-MS is ideally suited and has traditionally been used for analysis of nonpolar a作者: 可行 時間: 2025-3-24 05:03
NMR-Based Metabolomics for Biomarker Discovery,arly disease detection, patient stratification for treatment, and information on disease processes. With its ability to discover new metabolic markers, metabolomics (as well as metabolic profiling and metabonomics) is highly effective for drug development by providing early preclinical indications o作者: 談判 時間: 2025-3-24 10:25 作者: 無力更進 時間: 2025-3-24 11:26 作者: Psa617 時間: 2025-3-24 17:45
1557-2153 ndous urgency to address this question. Biomarkers also fit perfectly with the vision of personalized medicine, the new expectation of medical practice. This is why biomarker research has been a central focus i978-1-61737-925-3978-1-59745-463-6Series ISSN 1557-2153 Series E-ISSN 1940-6053 作者: 者變 時間: 2025-3-24 19:42 作者: 赦免 時間: 2025-3-24 23:17
Softwarestruktur eines Funktionsblocks,). LCM is a new technology that has substantially expanded our ability to examine cell-type–specific and region-specific responses in complex cellular tissues. Importantly, this technique allows the retrieval of specific cells from specific morphologic units within the tissue of interest..The primat作者: STALL 時間: 2025-3-25 05:06 作者: Ruptured-Disk 時間: 2025-3-25 08:36
,Konfliktbew?ltigung im V?lkerrecht,omatography–tandem mass spectrometry. Proteins are identified using public domain search engines, such as Mascot (Matrix Science Ltd., London, UK) and quantified using an in-house developed software package. During the fragmentation, the tag-labeled peptides generate a set of low mass reporters that作者: 火海 時間: 2025-3-25 14:29 作者: cocoon 時間: 2025-3-25 16:38
https://doi.org/10.1007/978-3-531-90009-4d metabolism that can improve the understanding of the health and disease processes. Current trends and recent advances in NMR-based metabolomics are focused on the development of advanced NMR methods, improved multivariate statistical data analysis, and a number of efforts to identify altered metab作者: foodstuff 時間: 2025-3-25 23:36 作者: GRAVE 時間: 2025-3-26 00:51
The Use of Two-Dimensional Gel Electrophoresis for Plasma Biomarker Discovery,ions provide detailed information on how to deplete high-abundant proteins from human plasma, apply the depleted plasma proteins to immobilized pH gradient gels, perform IEF and SDS-PAGE separation, and perform silver staining to visualize proteins. The basic 2-DE protocol described here could easil作者: Isolate 時間: 2025-3-26 07:28 作者: 門閂 時間: 2025-3-26 09:08
GC-MS-Based Metabolomics,ition, a brief introduction to gas chromatography and mass spectrometry will be given for readers who are less familiar with these subjects..GC-MS is distinctly different from the two other more established analytical methods that are used in metabolomics: nuclear magnetic resonance (NMR) and liquid作者: 灰心喪氣 時間: 2025-3-26 16:30
NMR-Based Metabolomics for Biomarker Discovery,d metabolism that can improve the understanding of the health and disease processes. Current trends and recent advances in NMR-based metabolomics are focused on the development of advanced NMR methods, improved multivariate statistical data analysis, and a number of efforts to identify altered metab作者: 不確定 時間: 2025-3-26 19:38 作者: Engaging 時間: 2025-3-26 21:15
Schlussbemerkungen und Ausblick, each stage of the measurement of a biomarker from collection through to assay. In this chapter, we present insight into some of these issues with the aim of increasing the general understanding of the use of biomarkers in clinical development.作者: gastritis 時間: 2025-3-27 02:36
Biomarker Sample Collection and Handling in the Clinical Setting to Support Early-Phase Drug Develo each stage of the measurement of a biomarker from collection through to assay. In this chapter, we present insight into some of these issues with the aim of increasing the general understanding of the use of biomarkers in clinical development.作者: Soliloquy 時間: 2025-3-27 06:16
Profiling Gene Expression Signatures Using Fluorescent Microspheres,ed, flow cytometric platform that allows the specific and sensitive screening of up to 100 transcripts per sample in a 96-well plate format. This chapter will outline experimental details of such an experiment using an estrogenic gene expression signature as a specific example.作者: AGATE 時間: 2025-3-27 12:33 作者: 抱狗不敢前 時間: 2025-3-27 16:10
Difference In-Gel Electrophoresis: ,,to perform it are described in detail within. The principles behind consistent gel image acquisition and reliable image analysis are also considered. Within the context of biomarker and drug target discovery, this method simplifies analysis, increases sample throughput, and represents a reliable 2-DE platform.作者: GUILE 時間: 2025-3-27 18:11
SELDI Technology for Identification of Protein Biomarkers,, ProteinChip arrays can be designed to characterize antibody-antigen and other protein-protein interactions. This review will update the reader on the current SELDI methods and protocols that are especially pertinent to protein biomarker discovery.作者: 控訴 時間: 2025-3-28 01:11
LC-MS Metabonomics Methodology in Biomarker Discovery,ivariate statistical methods are used to explore the complex metabolite patterns of biofluids. In this chapter, the methodology and application of high-performance liquid chromatography (HPLC)-MS–based metabonomics in biomarker discovery are reviewed.作者: 嚴重傷害 時間: 2025-3-28 03:06
1557-2153 methods for each of the different molecular levels.Real biomThe pharmaceutical industry has faced many significant challenges since the early 1990s. The fundamental issue that needs to be addressed is how to improve the efficiency of drug discovery and development. The current research and developme作者: Defraud 時間: 2025-3-28 07:18 作者: Pastry 時間: 2025-3-28 13:43 作者: 恃強凌弱 時間: 2025-3-28 14:47
Single Nucleotide Polymorphisms and DNA Methylation Analysis Using Pyrosequencing Methods,l controls for each sample. Pyrosequencing can also be used to identify triallelic, indel, and short-repeat polymorphisms, as well as determining allele percentages for DNA methylation or pooled sample assessment. Assays details for pyrosequencing of clinically relevant polymorphisms and DNA methylations are described in this chapter.作者: intrude 時間: 2025-3-28 21:02 作者: enflame 時間: 2025-3-29 01:25 作者: 使習(xí)慣于 時間: 2025-3-29 03:17
https://doi.org/10.1007/978-3-662-08149-5icular TaqMan.-based real-time PCR gene expression assays, provides a simple, robust, and practical tool that has shown great potential in bridging the gaps between biomarker discovery and clinical practice. In this chapter, we will survey the principle of this technology and its wide applications in biomarker discovery and validation.作者: Munificent 時間: 2025-3-29 08:50 作者: inculpate 時間: 2025-3-29 12:42
Rahmenbedingungen der Europ?isierung, ProteinChip arrays can be designed to characterize antibody-antigen and other protein-protein interactions. This review will update the reader on the current SELDI methods and protocols that are especially pertinent to protein biomarker discovery.作者: ONYM 時間: 2025-3-29 19:03
https://doi.org/10.1007/978-3-531-90009-4ivariate statistical methods are used to explore the complex metabolite patterns of biofluids. In this chapter, the methodology and application of high-performance liquid chromatography (HPLC)-MS–based metabonomics in biomarker discovery are reviewed.作者: Hot-Flash 時間: 2025-3-29 20:59 作者: 精致 時間: 2025-3-30 03:22
https://doi.org/10.1007/b138580capable of handling a large amount of data are essential in order to reduce the cost of discovery and increase the throughput. In this chapter, we will discuss and describe a case study of a unique proteomic method that uses a non-gel, label-free LC/MS-based protein quantification technology.作者: Clumsy 時間: 2025-3-30 04:53 作者: 神化怪物 時間: 2025-3-30 08:34 作者: OTTER 時間: 2025-3-30 14:58 作者: incite 時間: 2025-3-30 20:07 作者: Isthmus 時間: 2025-3-30 20:47
Schlussbemerkungen und Ausblick,the clinic of targeted therapeutics. This unprecedented opportunity to perform measures of human physiology responding to pharmacologic intervention requires an understanding of sample collection, handling (preanalytical variables), and assay qualification for the results to be informative and allow作者: 退出可食用 時間: 2025-3-31 03:24 作者: vascular 時間: 2025-3-31 07:20
Christian D. Middel,Patrick R. Staehlel conditions. Once these gene expression “signatures” have been elucidated, they can be used in screening assays to identify compounds that induce similar gene expression changes. To increase the throughput of these screening assays, we have optimized the development of a fluorescent microsphere–bas作者: 美色花錢 時間: 2025-3-31 09:11
https://doi.org/10.1007/978-3-662-08149-5creasingly important role in all phases of drug development as well as regulatory decision making. However, translating biomarker discovery into clinically useful tests in a time- and cost-effective manner remains a significant challenge. Real-time polymerase chain reaction (PCR) technology, in part作者: glans-penis 時間: 2025-3-31 14:28
Softwarestruktur eines Funktionsblocks,sequencing is a genotyping assay based on sequencing by synthesis. Short runs of sequence around each polymorphism are generated, allowing for internal controls for each sample. Pyrosequencing can also be used to identify triallelic, indel, and short-repeat polymorphisms, as well as determining alle作者: BATE 時間: 2025-3-31 18:07 作者: semble 時間: 2025-3-31 22:40 作者: 泰然自若 時間: 2025-4-1 05:08
Gliederung eines Bedienungssystems,orescent labels, has multiplex attributes, and boasts software-assisted image analysis. Combined, these characteristics offer significant benefits in accuracy and reproducibility to quantify differential protein expression levels between biological samples. The DIGE technique and materials required
