作者: 腐蝕 時(shí)間: 2025-3-22 00:07 作者: laparoscopy 時(shí)間: 2025-3-22 01:58
,Einladung zur Kryptokomplexit?t,nic activities, respectively. The bioreporter strains chromosomally express human estrogen receptor alpha (BLYES) or androgen receptor (BLYAS) and contain a reporter plasmid expressing the complete bacterial luciferase gene cassette (.) under the control of an estrogen- or androgen-responsive promot作者: 動(dòng)機(jī) 時(shí)間: 2025-3-22 06:09 作者: Gene408 時(shí)間: 2025-3-22 12:08 作者: 和藹 時(shí)間: 2025-3-22 14:42
https://doi.org/10.1007/978-3-7091-3416-0operon from . as a tool to analyze the progression of the fish pathogen . during the infection of rainbow trout, as well as the quantification of promoter activity of specific bacterial genes during host colonization.作者: 痛苦一生 時(shí)間: 2025-3-22 17:34 作者: 愚笨 時(shí)間: 2025-3-23 00:34 作者: 吞沒 時(shí)間: 2025-3-23 04:26 作者: 禍害隱伏 時(shí)間: 2025-3-23 07:07 作者: narcissism 時(shí)間: 2025-3-23 11:21
Komplikationen gibt es nicht - oder doch?e can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imagin作者: 可轉(zhuǎn)變 時(shí)間: 2025-3-23 16:42
Komplikationen gibt es nicht - oder doch?iruses in small animal models can allow for real-time analysis of the infection process without sacrificing the host, which has proven invaluable in the longitudinal study of disease events and testing the antiviral efficiencies of vaccine candidates, monoclonal antibodies and small molecule compoun作者: offense 時(shí)間: 2025-3-23 19:59
Lebertoxizit?t und volatile An?sthetikalanted cells within the complex environment of a living organism. However, this task can be daunting for even the most experienced researchers because it requires multiple animal handling steps and produces differential output signal characteristics in response to a number of experimental design var作者: BOAST 時(shí)間: 2025-3-23 23:19
https://doi.org/10.1007/978-3-642-97210-2 carry lipids, proteins, mRNAs, and miRNAs to enable intercellular communication between cells either locally or distantly without direct cell-to-cell contact. Cancer-derived EVs are known to facilitate tumor progression and metastasis by preparing premetastatic niches. Here, we define a strategy to作者: isotope 時(shí)間: 2025-3-24 05:07 作者: 出生 時(shí)間: 2025-3-24 09:42
Komplikationen in der An?sthesiety, excellent biocompatibility and real-time visualization and monitoring. The firefly luciferase (Fluc)/luciferin system, one of the principal bioluminescent systems, has been developed as a sensor for imaging biological processes. However, a limited number of Fluc substrates hamper the further app作者: 在前面 時(shí)間: 2025-3-24 14:44
Steven RippIncludes cutting-edge methods and protocols.Provides step-by-step details essential for reproducible results.Contains key notes and implementation advice from the experts作者: 拱形大橋 時(shí)間: 2025-3-24 18:09 作者: exceed 時(shí)間: 2025-3-24 21:54
Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced , Operon hampers the imaging of single cells. This chapter describes a procedure for the imaging of individual . cells using an improved . operon. The enhanced brightness of . enables long-term bioluminescence imaging of single bacteria with high sensitivity without the requirement for an external luciferin.作者: HEW 時(shí)間: 2025-3-25 00:54 作者: Dri727 時(shí)間: 2025-3-25 05:47 作者: 鈍劍 時(shí)間: 2025-3-25 07:40 作者: 指數(shù) 時(shí)間: 2025-3-25 12:26
Bioluminescent Imaging978-1-4939-9940-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: inspiration 時(shí)間: 2025-3-25 16:02 作者: 惡心 時(shí)間: 2025-3-25 23:27 作者: 消息靈通 時(shí)間: 2025-3-26 02:22
Komplikationen gibt es nicht - oder doch?ost important steps in preclinical research drug discovery before human clinical trials begin. Here we describe the use of the in vivo bioluminescent monitoring of high and low inocula of luciferase-expressing . (.) parasites in traditional and more innovative rodent models of in vivo cutaneous leishmaniasis (CL) drug discovery.作者: 流出 時(shí)間: 2025-3-26 06:33 作者: ESO 時(shí)間: 2025-3-26 11:32 作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-26 15:15 作者: 獸皮 時(shí)間: 2025-3-26 19:33
1064-3745 eadily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?Authoritative and cutting-edge, .Bioluminescent Imaging: Methods and Protocols .to ensure successful results in the further study of this vital field..978-1-4939-9942-2978-1-4939-9940-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 悶熱 時(shí)間: 2025-3-26 21:24 作者: SLAY 時(shí)間: 2025-3-27 03:06
Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Diseasey bioluminescence imaging in an animal model of PD. This method allows noninvasive tracking of the neuroinflammatory process that often precedes neurological signs of disease and represents an alternative to behavioral or histological and biochemical analysis to detect disease.作者: 經(jīng)典 時(shí)間: 2025-3-27 08:48
1064-3745 tation advice from the experts.This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful .Methods in Molec作者: gimmick 時(shí)間: 2025-3-27 09:37
Komplikationen gibt es nicht - oder doch?pression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R’s (replacement, reduction, and refinement) of biomedical animal research.作者: Salivary-Gland 時(shí)間: 2025-3-27 15:22 作者: ANTH 時(shí)間: 2025-3-27 20:00 作者: 中國(guó)紀(jì)念碑 時(shí)間: 2025-3-28 01:19 作者: Tracheotomy 時(shí)間: 2025-3-28 03:12 作者: 剝皮 時(shí)間: 2025-3-28 06:17
Book 2020 protocols, and tips on troubleshooting and avoiding known pitfalls..?Authoritative and cutting-edge, .Bioluminescent Imaging: Methods and Protocols .to ensure successful results in the further study of this vital field..作者: 錫箔紙 時(shí)間: 2025-3-28 13:58 作者: 就職 時(shí)間: 2025-3-28 16:24 作者: cochlea 時(shí)間: 2025-3-28 20:20
Komplexit?tstheorie und Kryptologieth high signal to background ratios. Here, we describe the synthesis and application of bioluminescence resonance energy transfer (BRET)-coupled quantum dots (QDs) for the NIR optical imaging of apoptotic cells.作者: 勉強(qiáng) 時(shí)間: 2025-3-29 00:33
,Einladung zur Kryptokomplexit?t,ose-dependent bioluminescence (i.e., light) emission. These yeast whole-cell bioassays provide rapid, cost-effective, and high-throughput detection of endocrine-disrupting activities in environmental samples. This protocol will provide a detailed description of the standard assay procedures as well as a framework for data analysis.作者: 無(wú)所不知 時(shí)間: 2025-3-29 03:57
Komplikationen gibt es nicht - oder doch? We also describe the widespread organ distribution of pMARV during infection by ex vivo imaging of necropsied tissues. This system can significantly facilitate Marburg virus studies and enable the evaluation of treatments against MARV in BSL-2 containments.作者: Amendment 時(shí)間: 2025-3-29 09:29
Komplikationen in der An?sthesierates (cyaLucs) that produced elevated bioluminescent signals in vitro. Furthermore, we demonstrate the high efficiency of .-cyclobutylaminoluciferin (cybLuc) with high light emission and long duration in deep tissue imaging by diagnosis of cerebral tumors in vivo in a rodent model.作者: Essential 時(shí)間: 2025-3-29 12:12 作者: RECUR 時(shí)間: 2025-3-29 19:02
High-Throughput Analysis of Endocrine-Disrupting Compounds Using BLYES and BLYAS Bioluminescent Yeasose-dependent bioluminescence (i.e., light) emission. These yeast whole-cell bioassays provide rapid, cost-effective, and high-throughput detection of endocrine-disrupting activities in environmental samples. This protocol will provide a detailed description of the standard assay procedures as well as a framework for data analysis.作者: 不再流行 時(shí)間: 2025-3-29 23:43
In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model We also describe the widespread organ distribution of pMARV during infection by ex vivo imaging of necropsied tissues. This system can significantly facilitate Marburg virus studies and enable the evaluation of treatments against MARV in BSL-2 containments.作者: chisel 時(shí)間: 2025-3-30 00:00
Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Modelsrates (cyaLucs) that produced elevated bioluminescent signals in vitro. Furthermore, we demonstrate the high efficiency of .-cyclobutylaminoluciferin (cybLuc) with high light emission and long duration in deep tissue imaging by diagnosis of cerebral tumors in vivo in a rodent model.作者: 我邪惡 時(shí)間: 2025-3-30 05:30 作者: 搏斗 時(shí)間: 2025-3-30 11:56
Komplikationen gibt es nicht - oder doch?ses in mice. The protocols described here are specifically tailored for use with oral streptococci, but the general strategies are adaptable for a wide range of polymicrobial infection studies using other species.作者: Boycott 時(shí)間: 2025-3-30 13:25
Komplikationen gibt es nicht - oder doch? of antifungal treatment. Bioluminescence imaging (BLI) made compatible with small animal models for in vivo biofilm formation is a valuable tool to follow biofilm development and its treatment longitudinally. Due to the noninvasive nature of BLI, the imaging procedure can be repeated in the same an作者: 某人 時(shí)間: 2025-3-30 16:32
Lebertoxizit?t und volatile An?sthetika demonstrate how cells implanted from a single injection can be imaged repeatedly across any post-implantation timescale without the need for further human–animal interaction or signal activation steps. This approach provides a facile means to continuously monitor implanted cellular output signals i作者: 諂媚于人 時(shí)間: 2025-3-31 00:38
Bioluminescent Imaging and Tracking of Bacterial Transport in Soilstribution of bioluminescent bacteria in the entire soil column is imaged. Finally, the images are processed to analyze bacterial migration in the soil under various environmental conditions in comparison with the breakthrough and elution curves of the bacteria obtained by analyzing the effluent samp作者: overreach 時(shí)間: 2025-3-31 00:57 作者: 干涉 時(shí)間: 2025-3-31 08:43
Bioluminescence Imaging to Study Mature Biofilm Formation by , spp. and Antifungal Activity In Vitro of antifungal treatment. Bioluminescence imaging (BLI) made compatible with small animal models for in vivo biofilm formation is a valuable tool to follow biofilm development and its treatment longitudinally. Due to the noninvasive nature of BLI, the imaging procedure can be repeated in the same an作者: 半圓鑿 時(shí)間: 2025-3-31 10:28
Continuous and Real-Time In Vivo Autobioluminescent Imaging in a Mouse Model demonstrate how cells implanted from a single injection can be imaged repeatedly across any post-implantation timescale without the need for further human–animal interaction or signal activation steps. This approach provides a facile means to continuously monitor implanted cellular output signals i作者: EVEN 時(shí)間: 2025-3-31 14:01
High-Throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lion and predictive data. Bioluminescent reporter gene assays and bioluminescence imaging in 3D cell models are very promising bioanalytical tools for several applications..Here we report a very straightforward method for bioluminescence imaging and bioluminescent reporter gene assays in 3D cell-cultu作者: hankering 時(shí)間: 2025-3-31 19:38
Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cellsprobes such as FITC-labeled Annexin V has been widely used for the detection of apoptotic cells. However, such probes cannot be applied to noninvasive imaging in the near-infrared (NIR) region. Compared with visible light, NIR light is highly permeable in turbid biological samples and tissues. In ad作者: PANEL 時(shí)間: 2025-3-31 22:47
