標(biāo)題: Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998 [打印本頁(yè)] 作者: otitis-externa 時(shí)間: 2025-3-21 18:28
書目名稱Bioluminescence Methods and Protocols影響因子(影響力)
書目名稱Bioluminescence Methods and Protocols影響因子(影響力)學(xué)科排名
書目名稱Bioluminescence Methods and Protocols網(wǎng)絡(luò)公開度
書目名稱Bioluminescence Methods and Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioluminescence Methods and Protocols被引頻次
書目名稱Bioluminescence Methods and Protocols被引頻次學(xué)科排名
書目名稱Bioluminescence Methods and Protocols年度引用
書目名稱Bioluminescence Methods and Protocols年度引用學(xué)科排名
書目名稱Bioluminescence Methods and Protocols讀者反饋
書目名稱Bioluminescence Methods and Protocols讀者反饋學(xué)科排名
作者: grovel 時(shí)間: 2025-3-21 21:41 作者: Thyroxine 時(shí)間: 2025-3-22 01:03
Das ?,ynergetic ,ntrepreneurship ,odel‘ plates are recommended for bright samples where reflection into neighboring wells results in “crosstalk.” White plates are recommended for samples that are lower light emitters, since the reflective surface enhances detection. Opaque plates are also available with transparent bottoms. Samples in the作者: 慎重 時(shí)間: 2025-3-22 06:25 作者: indubitable 時(shí)間: 2025-3-22 10:34 作者: 推崇 時(shí)間: 2025-3-22 15:36
Komplexit?t — das gez?hmte Chaosnse has been selected as a useful indicator of biological stress (.–.) because this response is universally found in btological systems and is induced by sublethal levels of a wide variety of cellular insults.作者: Neutropenia 時(shí)間: 2025-3-22 18:30
Visualization of Bioluminescenceplates are recommended for bright samples where reflection into neighboring wells results in “crosstalk.” White plates are recommended for samples that are lower light emitters, since the reflective surface enhances detection. Opaque plates are also available with transparent bottoms. Samples in the作者: 顯而易見 時(shí)間: 2025-3-22 22:36
Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Goneed noninvasively in livmg cells by either fluorescence microscopy, flow cytometry, or macroscopic imaging technrques. GFP has been used as a reporter in a wide range of species, including a number of different mammalian cell lines (.). Moreover, a variety of N-and C-terminal protein fusions with GFP作者: 合唱團(tuán) 時(shí)間: 2025-3-23 04:52
Genotoxic Sensors allows for the detection of agents that interact with DNA without a long incubation period. Many tests use . as the reporter for measuring transcriptional activation of promoters that are induced by DNA damage (.–.). In addition, .-prophage inductton assays (.,.) measures additional effects of the 作者: 采納 時(shí)間: 2025-3-23 07:38 作者: Enthralling 時(shí)間: 2025-3-23 12:21
Komplexit?t und Entrepreneurshipproduct or the disappearance of a substrate. Usually the compound measured is stable so that its concentration can be determined after a specific time. At low adenosine 5′triphosphate (ATP) concentrations, firefly luciferase is a stoichiometric reactant rather than a catalyst. In the case of the fir作者: 耕種 時(shí)間: 2025-3-23 15:40 作者: 不公開 時(shí)間: 2025-3-23 20:06 作者: 好開玩笑 時(shí)間: 2025-3-24 00:54
Abspann – Auszug aus einem Reiseberichtcor responding to few mg tissue). Since most intermediary metabolites are found in a concentratron range of ~50-500 μmol/g in a wide variety of tissues (., e.g., .. .), only nanomolar or subnanomolar quantities can be expected to be present in small samples obtained from isolated cells or cell cultu作者: FILLY 時(shí)間: 2025-3-24 04:21
Das ?,ynergetic ,ntrepreneurship ,odel‘ enzymes (.), and are allosteric regulators for such enzymes as glutamate dehydrogenase (.) and acetyl-CoA carboxylase (.). They regulate IMP dehydrogenase gene expression (.). The guanine nucleotldes are incorporated into DNA and RNA (.), are substrates in the Krebs tricarboxylic acid cycle (.), and作者: 不開心 時(shí)間: 2025-3-24 07:45
https://doi.org/10.1007/978-3-658-13173-9s (., .) and thereafter codified (.). This concept arose from making an analogy between the adenme nucleotide pool of ATP, ADP, and AMP, and a chemical storage battery or accumulator cell. The common elements include: (1) the total amount of material typically remains constant, and (2) chemical ener作者: 大約冬季 時(shí)間: 2025-3-24 10:43
Abspann – Auszug aus einem Reiseberichtbacterial systems, frequently used reporters include . (β-galactostdase), . (alkaline phosphatase), . (chloramphemcol transacetylase), and . (bacterial luciferase). Among these, the . reporter is distinct, because the reporter genes’ ultimate product, light, can be measured without disrupting the ce作者: 未成熟 時(shí)間: 2025-3-24 15:52 作者: 錯(cuò)事 時(shí)間: 2025-3-24 21:58 作者: uncertain 時(shí)間: 2025-3-25 00:04 作者: maudtin 時(shí)間: 2025-3-25 04:01
Komplexit?t von Entscheidungsproblemenve bloluminescenct activity. Although normally localized in peroxisomes, luciferase folds to the native state on expression in bacteria, in mammahan cells In culture (.), and in rabbit reticulocyte lysate (RRL) (.–.). RRL also efficiently facilitates the renaturation of thermally (.–.) or chemically作者: MITE 時(shí)間: 2025-3-25 11:27
Komplexit?t von IT-Architekturenwith carcinogeneity in humans (.). The activation of bacterial DNA repair systems (recently reviewed; .,.) can be used as a measure of mutagenic and genotoxic effects of various chemical as well as physical treatments. Many of the gene products that act to repair DNA, however, are difficult to assay作者: FAZE 時(shí)間: 2025-3-25 14:32
Komplexit?t — das gez?hmte Chaos (.). Several of the induced proteins function to reactivate or degrade denatured proteins, which are the signal initiating the response. The heat-shock response, therefore, functions to maintam the proteinacious component of the cell in an active form. Thus, by inducing heat-shock protein synthesis作者: Crohns-disease 時(shí)間: 2025-3-25 16:19 作者: 輪流 時(shí)間: 2025-3-25 21:00
https://doi.org/10.1007/978-3-658-05670-4based on the detection of adenosine triphosphate (ATP) are particularly useful, since it is possible to detect the ATP present in fewer than 20 cells/mL using optimized reagents. These same reagents can also accurately determine the ATP from 2 × 10. cells/mL in an adjacent well or test tube with a c作者: paleolithic 時(shí)間: 2025-3-26 02:50
Bioluminescence Methods and Protocols978-1-59259-577-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 發(fā)生 時(shí)間: 2025-3-26 06:37
Komplexit?t und Entrepreneurshipene expression in cases where protein product quantification is problematic. Reporters, such as /gbgalactostdase, provide convenient chromogenic assays (.). More recently, bioluminescence has been developed as a monitormg system (.). Reporter gene systems may be located extrachromosomally or integrated into the chromosome.作者: 拔出 時(shí)間: 2025-3-26 09:27
Komplexit?t von Entscheidungsproblemenving microorganisms for more than 50 years. There are numerous variations of the method, and elaborate equipment is available to improve environmental control and to allow large numbers of samples to be processed.作者: patriot 時(shí)間: 2025-3-26 15:56 作者: Pander 時(shí)間: 2025-3-26 20:34 作者: lanugo 時(shí)間: 2025-3-26 23:54
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187776.jpg作者: 預(yù)防注射 時(shí)間: 2025-3-27 04:33 作者: 失誤 時(shí)間: 2025-3-27 09:16
Cryopreservation and Reawakeningving microorganisms for more than 50 years. There are numerous variations of the method, and elaborate equipment is available to improve environmental control and to allow large numbers of samples to be processed.作者: 犬儒主義者 時(shí)間: 2025-3-27 09:37 作者: Fortuitous 時(shí)間: 2025-3-27 16:27
Improvements in the Application of Firefly Luciferase Assaysefly luciferase reaction, AMP, PP., CO., and oxyluciferin are typical products that accumulate, but the product that is most often and most easily determined is light. The photons of light are not accumulated in the measuring technique unless film or some electronic summation procedure is used in photon counting.作者: 分開如此和諧 時(shí)間: 2025-3-27 20:55
Bioluminescent Assay of the Adenylate Energy Chargegy can be stored and recovered by alteration of the ratio of the components. The charge of the three-component adenylate system is given by the mole fraction of ATP plus 1/2 of the mole fraction of ADP. It is shown in the following expression:作者: Ballad 時(shí)間: 2025-3-27 22:40
Promoter Probe Vectorsll. Furthermore, utthzatton of the five-gene . reporter system allows continuous monitoring of light production, An additional advantage of the . reporter is the sensitivity and large dynamic range of light detection equipment, which enables very weak to very strong transcripttonal acttvtty to be measured.作者: 座右銘 時(shí)間: 2025-3-28 06:10 作者: chapel 時(shí)間: 2025-3-28 08:17 作者: tic-douloureux 時(shí)間: 2025-3-28 11:37
Abspann – Auszug aus einem Reiseberichtene can be identified by mapping the transposon insertion site. However, not all genes encode an obvious phenotype or readily assayable product. By coupling transcription or translation to an easily assayed reporter gene, it is possible to monitor expression of any gene, even if its phenotype is not known a ..作者: rheumatology 時(shí)間: 2025-3-28 15:03 作者: Externalize 時(shí)間: 2025-3-28 22:27
Probing for Promoters with Luciferase-Transposonsene can be identified by mapping the transposon insertion site. However, not all genes encode an obvious phenotype or readily assayable product. By coupling transcription or translation to an easily assayed reporter gene, it is possible to monitor expression of any gene, even if its phenotype is not known a ..作者: 產(chǎn)生 時(shí)間: 2025-3-29 01:53
Komplexit?t und Entrepreneurshipefly luciferase reaction, AMP, PP., CO., and oxyluciferin are typical products that accumulate, but the product that is most often and most easily determined is light. The photons of light are not accumulated in the measuring technique unless film or some electronic summation procedure is used in photon counting.作者: CLAIM 時(shí)間: 2025-3-29 04:27
https://doi.org/10.1007/978-3-658-13173-9gy can be stored and recovered by alteration of the ratio of the components. The charge of the three-component adenylate system is given by the mole fraction of ATP plus 1/2 of the mole fraction of ADP. It is shown in the following expression:作者: 牛馬之尿 時(shí)間: 2025-3-29 10:05 作者: Hearten 時(shí)間: 2025-3-29 12:37
https://doi.org/10.1007/978-3-658-05670-4ompletely linear relationship between cell number and light output, provided that the cells maintain a relatively invariant ATP content. Similar methods are used for bacteria and eukaryotic cells, but this chapter is restricted to the consideration of eukaryotic cells. Luminescence measurement of ATP levels uses the following reaction:作者: MOTIF 時(shí)間: 2025-3-29 17:39
Komplexit?t von Entscheidungsproblemenlferase with guani-diniumHCl was thought to generate unfolded luciferase, which closely mimics nascent unfolded luciferase. However, recent results indicate that the folding pathway of chemically denatured luciferase is not identical to that followed by newly synthesized luciferase (.).作者: growth-factor 時(shí)間: 2025-3-29 23:33 作者: corn732 時(shí)間: 2025-3-30 00:37 作者: 儲(chǔ)備 時(shí)間: 2025-3-30 07:16 作者: 高興去去 時(shí)間: 2025-3-30 10:33 作者: subacute 時(shí)間: 2025-3-30 12:27
Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissues (., e.g., .. .), only nanomolar or subnanomolar quantities can be expected to be present in small samples obtained from isolated cells or cell cultures. Such quantities are at best barely detectable by conventional spectrophotometric or fluorimetric procedures作者: 可用 時(shí)間: 2025-3-30 17:23 作者: 半身雕像 時(shí)間: 2025-3-30 23:29
Visualization of Bioluminescenceist of different luminometers and cameras that are available. These instruments have been used to detect bioluminescence in a number of organisms using either bacterial luciferase (.; ., .) or firefly luciferase (.; ., .) as reporters. Sensitivity of the newer luminometers ranges from six to eight l作者: forager 時(shí)間: 2025-3-31 01:35
Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gonen, cell transformation, and cell lineage in VIVO and in real time. Unlike other bioluminescent reporters, the chromophore in GFP is mtrinsic to the primary structure of the protein, and GFP does not require additional factors other than molecular oxygen (..) to fluoresce (., .). GFP emits bright gre
