標(biāo)題: Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre [打印本頁] 作者: 小缺點(diǎn) 時間: 2025-3-21 19:47
書目名稱Bioluminescence影響因子(影響力)
書目名稱Bioluminescence影響因子(影響力)學(xué)科排名
書目名稱Bioluminescence網(wǎng)絡(luò)公開度
書目名稱Bioluminescence網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioluminescence被引頻次
書目名稱Bioluminescence被引頻次學(xué)科排名
書目名稱Bioluminescence年度引用
書目名稱Bioluminescence年度引用學(xué)科排名
書目名稱Bioluminescence讀者反饋
書目名稱Bioluminescence讀者反饋學(xué)科排名
作者: vibrant 時間: 2025-3-21 20:18
Aufbau der Arbeit und Vorgehensweisejugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.作者: Temporal-Lobe 時間: 2025-3-22 01:39
Arbeitsdefinition von Komplexit?tct interpretation of BLI results and validate BLI techniques. This chapter summarizes the use of calibrated light-emitting standards, bioluminescence tomography, and post-mortem validation of luciferase expression for validating quantitative BLI measurements.作者: Optimum 時間: 2025-3-22 04:33
Aufbau der Arbeit und Vorgehensweisesophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.作者: glucagon 時間: 2025-3-22 11:11 作者: 摘要記錄 時間: 2025-3-22 14:12
Günther Schuh,Hans-Peter Wiendahltrate sequence of caspase-3 (Asp-Glu-Val-Asp). When activated caspase-3 digests the substrate sequence, the cyclized luciferase recovers its activity. The indicator provides a general means of evaluating effects of cytotoxic compounds or novel pharmacological chemicals and apoptosis.作者: 使痛苦 時間: 2025-3-22 20:34 作者: 相符 時間: 2025-3-23 00:22 作者: 設(shè)想 時間: 2025-3-23 02:51
,Schlu?betrachtungen und Ausblick,er protein (PIN-G), containing HA, cMyc and GFP epitope, and fluorescence tags. Although originally designed for trafficking studies, pIN technology is a powerful tool applicable to almost every area of biology. Here we describe the methodologies used routinely in analyzing pIN constructs and some of their derivatives.作者: engender 時間: 2025-3-23 07:17 作者: Liability 時間: 2025-3-23 11:04 作者: 節(jié)約 時間: 2025-3-23 17:55
High-Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden,sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.作者: EWE 時間: 2025-3-23 19:40
,Fluorescence Imaging of Tumors with “Smart” pH-Activatable Targeted Probes,argeted probes can be applied to any target molecules on the cell surface that are to be internalized after ligand binding, this imaging strategy can afford a general and powerful method to diagnose and monitor the target tumors.作者: CUB 時間: 2025-3-24 00:22 作者: 助記 時間: 2025-3-24 03:11
Real-Time Bioluminescence Imaging of Viral Pathogenesis,tudies of viral infection in mouse models, we and others are using noninvasive bioluminescence imaging to track viral infection, dissemination, and effects of host immune mediators on disease. In this chapter, we detail experimental protocols for bioluminescence imaging of viral infections in living mice.作者: Rodent 時間: 2025-3-24 08:09 作者: 橡子 時間: 2025-3-24 11:11
PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins,er protein (PIN-G), containing HA, cMyc and GFP epitope, and fluorescence tags. Although originally designed for trafficking studies, pIN technology is a powerful tool applicable to almost every area of biology. Here we describe the methodologies used routinely in analyzing pIN constructs and some of their derivatives.作者: GRATE 時間: 2025-3-24 18:22
Book 2009Latest editionas endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bu作者: 配偶 時間: 2025-3-24 22:42 作者: duplicate 時間: 2025-3-25 03:03
https://doi.org/10.1007/978-3-322-81855-3 imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca. signals at the single-cell level.作者: 征稅 時間: 2025-3-25 06:12 作者: FACT 時間: 2025-3-25 10:35
Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles, imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca. signals at the single-cell level.作者: 古代 時間: 2025-3-25 13:36
Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays,e also show how they can be combined with protein complementation assays such as bimolecular fluorescence complementation (BiFC) to study three- and four-partner interactions. We also describe a BRET assay that uses SNAP-tagged proteins as a fluorescence acceptor molecule for the bioluminescent donor.作者: ventilate 時間: 2025-3-25 19:24 作者: 取回 時間: 2025-3-25 22:29
Günther Schuh,Hans-Peter Wiendahlative luciferases, such as those from the firefly (.) or marine copepods (.), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are .-, .-, or .-positive.作者: keloid 時間: 2025-3-26 00:24 作者: llibretto 時間: 2025-3-26 07:53
J?rn-Henrik von Thun,Joachim Stumpfenase under the control of the same SBE promoter (SBE-lucRT mice). SBE-luc and SBE-lucRT mice can be used to study temporal, tissue-specific activation of Smad2/3-dependent signaling in living mice as well as for the identification of endogenous or synthetic modulators of this pathway.作者: 充氣女 時間: 2025-3-26 10:16 作者: 少量 時間: 2025-3-26 13:47
Bioluminescent Monitoring of In Vivo Colonization and Clearance Dynamics by Light-Emitting Bacteriaative luciferases, such as those from the firefly (.) or marine copepods (.), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are .-, .-, or .-positive.作者: 群島 時間: 2025-3-26 17:54
Quantitative In Vivo Imaging of Non-viral-Mediated Gene Expression and RNAi-Mediated Knockdown,mary advantages of using quantitative BLI in mouse liver and muscle are the sensitivity of the assay, the speed and ease of making measurements, the precision and linearity of the dose–response curves, and the ability to conduct serial sampling of gene expression over many days or months while eliminating the need to euthanize animals.作者: explicit 時間: 2025-3-27 01:00 作者: 螢火蟲 時間: 2025-3-27 03:29
,Analysis of Protein–Protein Interactions Using Bioluminescence Resonance Energy Transfer, these fusion proteins is then detected when they are in close proximity, indicative of association either directly or as part of a complex. BRET is particularly useful for real-time monitoring of ligand-modulated interactions as dynamic changes in protein complex assembly can be observed in a live cell environment.作者: Myosin 時間: 2025-3-27 08:58 作者: 無法取消 時間: 2025-3-27 10:42 作者: 藥物 時間: 2025-3-27 14:41 作者: 我不死扛 時間: 2025-3-27 21:11 作者: abysmal 時間: 2025-3-28 01:00
Preston B. Rich,Christelle DouilletProvides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field.Comprehensive guide to developing, validating, ru作者: 太空 時間: 2025-3-28 06:00
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187775.jpg作者: 厭惡 時間: 2025-3-28 07:35
Aufbau der Arbeit und Vorgehensweisee discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, ., ., and . luciferases, and calcium-activated photoproteins. Accordi作者: ABYSS 時間: 2025-3-28 11:07
Arbeitsdefinition von Komplexit?tesearch, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability a作者: outer-ear 時間: 2025-3-28 18:26 作者: Duodenitis 時間: 2025-3-28 21:21
Aufbau der Arbeit und Vorgehensweisead in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing lit作者: 溫和女人 時間: 2025-3-29 02:04
Komplexit?t interner Dienstleistungenon-target tissues. In this study, a “smart” activatable strategy for specific tumor imaging is proposed in which pH-activatable targeted probes specifically detect tumors after binding to the target cell surface proteins, internalization, and eventual acidic pH activation within the acidic organelle作者: 下垂 時間: 2025-3-29 04:46
https://doi.org/10.1007/978-3-658-11512-8mitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.作者: START 時間: 2025-3-29 09:38
https://doi.org/10.1007/978-3-642-60841-4se, including monitoring the fate of transplanted islets in vivo in real time. In order to advance our understanding of the pathophysiology and immunobiology of islet transplantation as they occur in living animals, islet grafts tagged with light-emitting luciferase can be implanted in a mouse islet作者: 甜瓜 時間: 2025-3-29 14:33 作者: vocation 時間: 2025-3-29 19:16 作者: 牢騷 時間: 2025-3-29 20:41
https://doi.org/10.1007/978-3-642-60841-4imals to determine viral or bacterial distributions and titers. The recent application of the in vivo bioluminescence imaging (BLI) to monitor the replication and tropism of pathogens expressing the luciferase (from firefly or .) reporter proteins has been recently developed. This technology do not 作者: 保留 時間: 2025-3-30 01:05
Kernf?higkeit: Wissensmanagements of viral infection in mice rely upon euthanizing cohorts of animals at multiple time points to identify sites of infection, quantify viral titers, and determine host immune responses. This experimental paradigm precludes longitudinal studies of infection and response to treatment in the same anima作者: 撫慰 時間: 2025-3-30 07:34
Günther Schuh,Hans-Peter Wiendahlrendered bioluminescent, allowing the sensitive detection of bacterial burden and metabolic activity in real-time and in situ in living animals. In this chapter we describe the protocols for characterizing in vivo infection models using bioluminescent bacteria: from real-time imaging in living anima作者: PANEL 時間: 2025-3-30 10:53 作者: 刀鋒 時間: 2025-3-30 14:54 作者: ETCH 時間: 2025-3-30 17:57 作者: 變化 時間: 2025-3-30 21:18 作者: 合乎習(xí)俗 時間: 2025-3-31 02:02 作者: 鋼筆尖 時間: 2025-3-31 05:44
,Schlu?betrachtungen und Ausblick,s in living cells under physiological conditions. Here we describe protocols for these assays including relevant positive and negative controls, and we also show how they can be combined with protein complementation assays such as bimolecular fluorescence complementation (BiFC) to study three- and f作者: 藥物 時間: 2025-3-31 10:30 作者: 商品 時間: 2025-3-31 13:41
Luminescent Probes and Visualization of Bioluminescence,e discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, ., ., and . luciferases, and calcium-activated photoproteins. Accordi作者: 壟斷 時間: 2025-3-31 18:47
Validation of Bioluminescent Imaging Techniques,esearch, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability a作者: 帶子 時間: 2025-3-31 22:59
Assessment of Extracellular ATP Concentrations,l surface purinergic receptors (purinoceptors). Biological responses regulated by purinergic receptors include neurotransmission, smooth muscle relaxation and contraction, epithelial cell ion transport, inflammation, platelet activation, immune responses, cardiac function, endocrine and exocrine sec作者: obnoxious 時間: 2025-4-1 04:32 作者: coalition 時間: 2025-4-1 07:23
,Fluorescence Imaging of Tumors with “Smart” pH-Activatable Targeted Probes,on-target tissues. In this study, a “smart” activatable strategy for specific tumor imaging is proposed in which pH-activatable targeted probes specifically detect tumors after binding to the target cell surface proteins, internalization, and eventual acidic pH activation within the acidic organelle作者: Fibrinogen 時間: 2025-4-1 12:18 作者: cliche 時間: 2025-4-1 17:10 作者: 松軟無力 時間: 2025-4-1 20:56 作者: bibliophile 時間: 2025-4-2 01:47 作者: 金盤是高原 時間: 2025-4-2 05:04
Noninvasive Bioluminescent Imaging of Infections,imals to determine viral or bacterial distributions and titers. The recent application of the in vivo bioluminescence imaging (BLI) to monitor the replication and tropism of pathogens expressing the luciferase (from firefly or .) reporter proteins has been recently developed. This technology do not 作者: electrolyte 時間: 2025-4-2 10:36
Real-Time Bioluminescence Imaging of Viral Pathogenesis,s of viral infection in mice rely upon euthanizing cohorts of animals at multiple time points to identify sites of infection, quantify viral titers, and determine host immune responses. This experimental paradigm precludes longitudinal studies of infection and response to treatment in the same anima
