標題: Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁] 作者: Fatuous 時間: 2025-3-21 18:44
書目名稱Bioluminescence影響因子(影響力)
書目名稱Bioluminescence影響因子(影響力)學(xué)科排名
書目名稱Bioluminescence網(wǎng)絡(luò)公開度
書目名稱Bioluminescence網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioluminescence被引頻次
書目名稱Bioluminescence被引頻次學(xué)科排名
書目名稱Bioluminescence年度引用
書目名稱Bioluminescence年度引用學(xué)科排名
書目名稱Bioluminescence讀者反饋
書目名稱Bioluminescence讀者反饋學(xué)科排名
作者: 哄騙 時間: 2025-3-21 22:37 作者: 除草劑 時間: 2025-3-22 02:16
Visible Light Bioluminescence Imaging Platform for Animal Cell Imagingerase sets. We exemplify to create diverse hues of bioluminescence (BL) ranging from blue to far red with the combination of marine luciferases and the three groups of CTZ analogues. We also show how to characterize the new CTZ analogues in detail such as the kinetic parameters, dose dependency, and作者: 輕推 時間: 2025-3-22 08:03
Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Unfortunately, luciferin racemizes easily and accumulation of nonluminous L-luciferin has negative influences on the light-emitting reaction. By a detailed analysis of luciferin chirality, however, it becomes clarified that L-luciferin is the biosynthetic precursor of D-luciferin in fireflies and un作者: CHOIR 時間: 2025-3-22 12:13 作者: 桶去微染 時間: 2025-3-22 14:11
Production of Copepod Luciferases via Baculovirus Expression Systemazine, with the emission of blue light (.?=?485–488?nm), that is called bioluminescence (BL). The well-studied Gaussia (GLuc) and Metridia (MLuc) luciferases originally cloned from the marine copepods . and . belong to the group of the smallest natural luciferases. Their minimal molecular weight, hi作者: inhibit 時間: 2025-3-22 19:41
Molecular Tension Probe for In Vitro Bioassaysong-term utility, and (iii) bioanalytical versatility. The authors previously introduced a unique bioluminescent imaging probe for illuminating molecular tension appended by protein-protein interactions (PPIs) of interest. In this chapter, we exemplify that a full-length artificial luciferase is san作者: pacifist 時間: 2025-3-22 23:16
Optimized Loop-Mediated Isothermal Amplification (LAMP) Allows Single Copy Detection Using Biolumine The key component for BART is a thermostable luciferase derived from the firefly .. Coupling BART detection with the isothermal amplification method loop-mediated isothermal amplification (LAMP) enables rapid molecular diagnostic results using simple equipment. LAMP-BART provides quantitative resul作者: linear 時間: 2025-3-23 02:40
A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a D-Lucie the inhibition of OAT1 function by a concomitant drug may cause pharmacokinetic drug-drug interactions (DDIs) in clinical practice, an in vitro uptake study to evaluate the inhibition potency of OAT1 is useful for the prediction and avoidance of DDIs and recommended for drug candidates in drug dev作者: Fulsome 時間: 2025-3-23 09:07 作者: 流逝 時間: 2025-3-23 11:06
A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening Against Infective African Trphic and infective (bloodstream) form of .. The assay relies on a bioluminescent transgenic parasite harboring a genetically encoded copy of a thermostable redshifted firefly?luciferase from ...The major advantages of the assay are simplicity and cost efficiency, along with excellent quality parame作者: echnic 時間: 2025-3-23 14:33 作者: 積習(xí)難改 時間: 2025-3-23 21:27 作者: penance 時間: 2025-3-24 00:55 作者: Confound 時間: 2025-3-24 04:27 作者: Ptsd429 時間: 2025-3-24 10:13
Design of an Intron-Retained Bioluminescence Reporter and its Application in Imaging of Pre-mRNA Spl with abnormal splicing of pre-mRNA. Conventional methods require sample lysis and thus cannot be used for monitoring pre-mRNA splicing in real time. This chapter guides how to develop an intron-retained bioluminescence (BL) reporter, which simulates the splicing process of pre-mRNA in vitro and in 作者: 言外之意 時間: 2025-3-24 14:15
Generation of Bi-Reporter-Expressing Tri-Segmented Arenavirusirus life cycle and also for the discovery of antiviral countermeasures. The plasmid-encoded viral ribonucleoprotein components initiate the transcription and replication of a plasmid-driven full-length viral genome, resulting in infectious virus. Thereby, this approach is ideal for the generation o作者: jeopardize 時間: 2025-3-24 16:16 作者: Fulsome 時間: 2025-3-24 20:05 作者: 配置 時間: 2025-3-25 02:37
https://doi.org/10.1007/978-3-658-09972-5cal phenomena both in vitro and in vivo. Coelenterazine (CTZ), the known widespread luciferin found in bioluminescent organisms, develops bioluminescence imaging (BLI). Here, we describe an approach to synthesize a series of novel CTZ derivatives for diversifying the toolbox of the BL substrates. Fu作者: 令人不快 時間: 2025-3-25 06:31
,Implikationen für Praxis und Wissenschaft,erase sets. We exemplify to create diverse hues of bioluminescence (BL) ranging from blue to far red with the combination of marine luciferases and the three groups of CTZ analogues. We also show how to characterize the new CTZ analogues in detail such as the kinetic parameters, dose dependency, and作者: CLOWN 時間: 2025-3-25 09:58 作者: 被告 時間: 2025-3-25 12:35 作者: 輕率的你 時間: 2025-3-25 18:53 作者: 小教堂 時間: 2025-3-25 22:26 作者: 典型 時間: 2025-3-26 01:03 作者: 得意牛 時間: 2025-3-26 05:24
Aufbau der Arbeit und Vorgehensweisee the inhibition of OAT1 function by a concomitant drug may cause pharmacokinetic drug-drug interactions (DDIs) in clinical practice, an in vitro uptake study to evaluate the inhibition potency of OAT1 is useful for the prediction and avoidance of DDIs and recommended for drug candidates in drug dev作者: 牙齒 時間: 2025-3-26 12:31
Aufbau der Arbeit und Vorgehensweiseinescence (BL) signal. The assay uses a reporter cell line of . that expresses constitutively a redshifted luciferase from . and murine macrophages (cell line J774.A1) as host cells for infection. The host cell line was selected because it is a differentiated cell line, easy to manipulate in vitro, 作者: Dri727 時間: 2025-3-26 13:28
https://doi.org/10.1007/978-3-8350-5403-5rphic and infective (bloodstream) form of .. The assay relies on a bioluminescent transgenic parasite harboring a genetically encoded copy of a thermostable redshifted firefly?luciferase from ...The major advantages of the assay are simplicity and cost efficiency, along with excellent quality parame作者: 使?jié)M足 時間: 2025-3-26 20:07
https://doi.org/10.1007/978-3-8350-5403-5oscopic imaging of single cells by their bioluminescence (BL) emission generated without an external luciferin. Imaging is based on the expression of codon-optimized . (co .) genes and does not require manipulation of the cells apart from transfection. Due to the high brightness of the co . system, 作者: 痛恨 時間: 2025-3-26 22:36 作者: monologue 時間: 2025-3-27 03:55 作者: 不適當 時間: 2025-3-27 07:22
Aufbau der Arbeit und VorgehensweiseRs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5′ untranslated region (5′ UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulat作者: 混亂生活 時間: 2025-3-27 12:52 作者: Antecedent 時間: 2025-3-27 14:28
Aufbau der Arbeit und Vorgehensweiseirus life cycle and also for the discovery of antiviral countermeasures. The plasmid-encoded viral ribonucleoprotein components initiate the transcription and replication of a plasmid-driven full-length viral genome, resulting in infectious virus. Thereby, this approach is ideal for the generation o作者: Ergots 時間: 2025-3-27 18:19 作者: 西瓜 時間: 2025-3-28 00:04 作者: 幸福愉悅感 時間: 2025-3-28 04:32
https://doi.org/10.1007/978-1-0716-2453-1Chemiluminescence; Molecular imaging; Bioanalysis; Luciferins; Luciferase; Multiplex imaging platforms作者: Thyroiditis 時間: 2025-3-28 09:11
978-1-0716-2455-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Range-Of-Motion 時間: 2025-3-28 11:12 作者: 燈泡 時間: 2025-3-28 15:22
Production of Metridia Luciferase in Native Form by Oxidative Refolding from , Inclusion Bodies disulfide bonds, and refolding to the native state using a glutathione redox system in the presence of arginine and Cu. ions and (ii)?chromatographic purification of MLuc and its functional assessment in terms of activity. We introduce the empirical, optimal conditions for oxidative refolding and s作者: 法律 時間: 2025-3-28 20:29 作者: Concomitant 時間: 2025-3-28 23:05 作者: Constitution 時間: 2025-3-29 06:49 作者: Indolent 時間: 2025-3-29 07:33 作者: 鴿子 時間: 2025-3-29 12:08
Arbeitsdefinition von Komplexit?t disulfide bonds, and refolding to the native state using a glutathione redox system in the presence of arginine and Cu. ions and (ii)?chromatographic purification of MLuc and its functional assessment in terms of activity. We introduce the empirical, optimal conditions for oxidative refolding and s作者: CIS 時間: 2025-3-29 16:56
https://doi.org/10.1007/978-3-8350-5403-5molecule). Here, we describe the universal protocol for highly effective secreted expression of disulfide-rich CopLucs using their own signal peptide in insect cells and their purification from serum-free culture medium. The suggested protocol allows obtaining high-purity CopLucs folded in their nat作者: Venules 時間: 2025-3-29 21:25 作者: Nomadic 時間: 2025-3-30 00:17 作者: 仔細檢查 時間: 2025-3-30 06:07
1064-3745 ents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories..978-1-0716-2455-5978-1-0716-2453-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 征服 時間: 2025-3-30 11:53 作者: 偉大 時間: 2025-3-30 12:27 作者: 易受騙 時間: 2025-3-30 17:15 作者: 贊美者 時間: 2025-3-30 20:47
https://doi.org/10.1007/978-3-8350-5403-5grown in the presence of the molecules of interest in a 96-well microplate, and 24?h later, BL is determined with a simple protocol lacking washing steps, using cost-efficient reagents with a reasonable readout time for high-throughput applications.作者: 生來 時間: 2025-3-31 01:13
https://doi.org/10.1007/978-3-8350-5403-5tion and then culturing the filter in an agar medium, the beer-derived ATP remaining on the filter can be removed and the ATP of LAB cells is increased. As a result, the signal-to-noise ratio of the RMD method can be dramatically improved.作者: carbohydrate 時間: 2025-3-31 09:06 作者: goodwill 時間: 2025-3-31 09:36
A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules Against the Intracellular For microplate and a 24?h treatment. The assay setup shows excellent balance between simplicity (cell culture manipulation/infection and timing) and quality parameters, as well as potential to detect drug-like molecules acting in a fast and cytotoxic manner.作者: visual-cortex 時間: 2025-3-31 15:04
