標(biāo)題: Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical [打印本頁(yè)] 作者: ONSET 時(shí)間: 2025-3-21 19:17
書目名稱Bioluminescence影響因子(影響力)
書目名稱Bioluminescence影響因子(影響力)學(xué)科排名
書目名稱Bioluminescence網(wǎng)絡(luò)公開度
書目名稱Bioluminescence網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioluminescence被引頻次
書目名稱Bioluminescence被引頻次學(xué)科排名
書目名稱Bioluminescence年度引用
書目名稱Bioluminescence年度引用學(xué)科排名
書目名稱Bioluminescence讀者反饋
書目名稱Bioluminescence讀者反饋學(xué)科排名
作者: 感激小女 時(shí)間: 2025-3-21 22:49
Men’s Issues and Men’s Mental Healthdrugs and to preclinically evaluate them in living animals. Method detects and quantitatively monitors the level of histone methylations in intact cells, is of a prerequisite to screen small molecules that modulate histone lysine methylation. Here, we describe two independent optical imaging sensors作者: 攀登 時(shí)間: 2025-3-22 04:28
1064-3745 ing and avoiding known pitfalls...Authoritative and practical, .Bioluminescence: Methods and Protocols, Third Edition. is a usefulcomplement to the first and second edition for new and experienced researchersalike..?.978-1-4939-8146-5978-1-4939-3813-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Institution 時(shí)間: 2025-3-22 04:55 作者: Entreaty 時(shí)間: 2025-3-22 12:22
Imaging Histone Methylations in Living Animalsdrugs and to preclinically evaluate them in living animals. Method detects and quantitatively monitors the level of histone methylations in intact cells, is of a prerequisite to screen small molecules that modulate histone lysine methylation. Here, we describe two independent optical imaging sensors作者: 鬧劇 時(shí)間: 2025-3-22 13:16 作者: 遺棄 時(shí)間: 2025-3-22 18:55
https://doi.org/10.1007/978-3-531-91140-3ssible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by . luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compou作者: vanquish 時(shí)間: 2025-3-22 23:36 作者: MONY 時(shí)間: 2025-3-23 04:25
Zusammenfassung und Schlussfolgerungenciferase sequences. The made ALucs have unique sequential identities that are phylogenetically distinctive from those of any existing copepod luciferase. Some ALucs exhibited heat stability, and strong and greatly prolonged optical intensities. The made ALucs are applicable to various bioassays as a作者: CLOT 時(shí)間: 2025-3-23 08:11
Elias’ theoretisch-empirischer Rahmenacterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine . according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are i作者: 真實(shí)的你 時(shí)間: 2025-3-23 12:57
Elias’ theoretisch-empirischer Rahmen applied bioluminescence to ligand–receptor binding assays for some protein or peptide hormones based on a newly developed small monomeric Nanoluciferase (NanoLuc) reporter that has the so far brightest bioluminescence. The conventional ligand–receptor binding assays rely on radioligands that have d作者: 原來(lái) 時(shí)間: 2025-3-23 15:01 作者: GUEER 時(shí)間: 2025-3-23 21:50 作者: 潔凈 時(shí)間: 2025-3-24 00:39 作者: Fortuitous 時(shí)間: 2025-3-24 04:12
Michael Snowden,Jamie P. Halsallion assay (PCA) is one of the most widely used methods to detect PPI by splitting the enzyme-coding or fluorescent protein-coding polypeptide, as well as F?rster resonance energy transfer (FRET). Here, we describe a novel PPI assay FlimPIA (.irefly .uminescent .nter.ediate-based .rotein–protein .nte作者: GULLY 時(shí)間: 2025-3-24 06:39
https://doi.org/10.1057/9780230227620minating androgenicity of ligands. In the single-chain probe, a functional peptide in the AR NTD was genetically fused to the ligand-binding domain of AR (AR LBD) via a flexible linker, and then sandwiched between the N- and C-terminal fragments of split-firefly luciferase (FLuc) dissected at D415. 作者: 禍害隱伏 時(shí)間: 2025-3-24 11:00 作者: 慢跑 時(shí)間: 2025-3-24 16:03 作者: RENAL 時(shí)間: 2025-3-24 19:35 作者: 有特色 時(shí)間: 2025-3-24 23:31 作者: 起來(lái)了 時(shí)間: 2025-3-25 04:57
https://doi.org/10.1007/978-3-030-86320-3or GPCR signaling in a high-throughput manner. However, information related to the temporal reaction of GPCR with specific ligands remains poor. We recently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complem作者: Obsequious 時(shí)間: 2025-3-25 10:35 作者: FAWN 時(shí)間: 2025-3-25 15:18
Bioluminescence978-1-4939-3813-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Efflorescent 時(shí)間: 2025-3-25 18:23
https://doi.org/10.1007/978-3-531-91140-3ssible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by . luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC?.作者: 單純 時(shí)間: 2025-3-25 23:15
Elias’ theoretisch-empirischer Rahmenacterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine . according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.作者: 陪審團(tuán)每個(gè)人 時(shí)間: 2025-3-26 02:58 作者: TOXIC 時(shí)間: 2025-3-26 07:41 作者: 深陷 時(shí)間: 2025-3-26 10:31 作者: 撕裂皮肉 時(shí)間: 2025-3-26 15:01
Sung Bae KimIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: 琺瑯 時(shí)間: 2025-3-26 17:52 作者: 小溪 時(shí)間: 2025-3-26 23:44
Synthetic Bioluminescent Coelenterazine Derivativesssible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by . luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC?.作者: 個(gè)人長(zhǎng)篇演說(shuō) 時(shí)間: 2025-3-27 02:16
Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescencacterize luminescence light intensity emitted diffusively from the reaction solution mixture. Here, methods to measure number of photons to determine . according to the techniques of national radiometry standards are described. As an example, experiments using firefly bioluminescence reactions are introduced.作者: 心胸狹窄 時(shí)間: 2025-3-27 07:32 作者: 一個(gè)攪動(dòng)不安 時(shí)間: 2025-3-27 10:37
Book 2016Latest editionreagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practical, .Bioluminescence: Methods and Protocols, Third Edition. is a usefulcomplement to the first and second edition for new and experienced researchersalike..?.作者: Stress 時(shí)間: 2025-3-27 17:26 作者: 胖人手藝好 時(shí)間: 2025-3-27 21:39 作者: cloture 時(shí)間: 2025-3-27 23:07
Marianne Inéz Lien,J?rgen Lorentzenroximation of the adjacent fragments of split-cpLuc recovering the enzyme activity. This probe design greatly improves signal-to-noise (S/N) ratios upon tracing weak protein–protein interactions (PPIs) in mammalian cells.作者: Graduated 時(shí)間: 2025-3-28 04:51
Bioluminescent Ligand–Receptor Binding Assays for Protein or Peptide Hormones protein or peptide hormones for ligand–receptor interaction studies in future. In the present article, we provided detailed protocols for setting up the novel bioluminescent ligand–receptor binding assays using two representative protein hormones as examples.作者: expdient 時(shí)間: 2025-3-28 07:57 作者: concubine 時(shí)間: 2025-3-28 14:13 作者: 擦掉 時(shí)間: 2025-3-28 17:22 作者: 斷斷續(xù)續(xù) 時(shí)間: 2025-3-28 20:47 作者: 四目在模仿 時(shí)間: 2025-3-29 02:16 作者: 生命 時(shí)間: 2025-3-29 04:39 作者: 稱贊 時(shí)間: 2025-3-29 08:21 作者: 淺灘 時(shí)間: 2025-3-29 11:35
Zusammenfassung und Schlussfolgerungeneptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.作者: 王得到 時(shí)間: 2025-3-29 17:59
Michael Snowden,Jamie P. Halsallin exhibits pH dependence in vitro. When expressed in either yeast or mammalian cells, pHlash reports basal pH and cytosolic acidification. In this chapter, we describe an in vitro characterization of pHlash, and also in vivo assays including in yeast cells and in HeLa cells using pHlash as a cytoplasmic pH indicator.作者: faculty 時(shí)間: 2025-3-29 22:14 作者: 畸形 時(shí)間: 2025-3-30 01:31 作者: flutter 時(shí)間: 2025-3-30 04:57
Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivoeptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.作者: homeostasis 時(shí)間: 2025-3-30 10:43
Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor inin exhibits pH dependence in vitro. When expressed in either yeast or mammalian cells, pHlash reports basal pH and cytosolic acidification. In this chapter, we describe an in vitro characterization of pHlash, and also in vivo assays including in yeast cells and in HeLa cells using pHlash as a cytoplasmic pH indicator.作者: 出來(lái) 時(shí)間: 2025-3-30 15:46 作者: 褻瀆 時(shí)間: 2025-3-30 18:38
Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-Coupled Receptor for High-Througcently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complementation. To monitor time-course variation of the interactions, a new imaging system contributes to the accurate evaluation of drugs for GPCRs in a high-throughput manner.作者: Legion 時(shí)間: 2025-3-30 23:23 作者: Foreknowledge 時(shí)間: 2025-3-31 03:48 作者: N防腐劑 時(shí)間: 2025-3-31 07:28
Zusammenfassung und Schlussfolgerungenn optical readout, including live cell imaging, single-chain probes, and bioluminescent tags of antibodies. The present protocol guides on how to fabricate a unique artificial luciferase with designed optical properties and functionalities.作者: 嘮叨 時(shí)間: 2025-3-31 09:26 作者: exceptional 時(shí)間: 2025-3-31 16:07
Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepodstified copepod luciferases from bioluminescent copepod crustaceans. Here, we introduce how to establish a cDNA encoding novel copepod luciferases from a copepod specimen by PCR with degenerated primers.
