標題: Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive [打印本頁] 作者: 極大 時間: 2025-3-21 16:26
書目名稱Bioluminescence影響因子(影響力)
書目名稱Bioluminescence影響因子(影響力)學科排名
書目名稱Bioluminescence網(wǎng)絡公開度
書目名稱Bioluminescence網(wǎng)絡公開度學科排名
書目名稱Bioluminescence被引頻次
書目名稱Bioluminescence被引頻次學科排名
書目名稱Bioluminescence年度引用
書目名稱Bioluminescence年度引用學科排名
書目名稱Bioluminescence讀者反饋
書目名稱Bioluminescence讀者反饋學科排名
作者: 整潔漂亮 時間: 2025-3-21 23:46
https://doi.org/10.1007/978-3-531-91181-6tatively assess the efficacy of NIR-PIT for both in vitro and in vivo experiments, especially in the early phase of testing. Here, we describe the detailed methods of the experiments for NIR-PIT and evaluation of its efficacy using BLI.作者: macrophage 時間: 2025-3-22 01:05 作者: 譏笑 時間: 2025-3-22 08:06
Clare Woolhouse,Laura J. Nicholsonables the screening of modulators of essential PPIs for . survival. Considering the complexity of the evaluated mixture, pure chemical compounds or natural extracts, two approaches are described, BRET in living cells or from lysates.作者: 拖網(wǎng) 時間: 2025-3-22 12:16
Quantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imatatively assess the efficacy of NIR-PIT for both in vitro and in vivo experiments, especially in the early phase of testing. Here, we describe the detailed methods of the experiments for NIR-PIT and evaluation of its efficacy using BLI.作者: 連鎖 時間: 2025-3-22 15:49 作者: 催眠藥 時間: 2025-3-22 17:55
Screening of Protein–Protein Interaction Modulators Using BRET-Based Technologyables the screening of modulators of essential PPIs for . survival. Considering the complexity of the evaluated mixture, pure chemical compounds or natural extracts, two approaches are described, BRET in living cells or from lysates.作者: 陶醉 時間: 2025-3-22 23:09
https://doi.org/10.1007/978-3-531-91181-6ressing enzymes, such as the bioluminescent luciferase, can also be captured to the DNA origami breadboard with similar precision. DNA nanostructure conjugation of the QDs or luciferase is confirmed through imaging and/or energy transfer to organic dyes integrated into the DNA nanostructure.作者: 碎石 時間: 2025-3-23 01:55
Mentoring Diversity for Inclusion and Equitynces without perturbing their natural environment, which we call MERLIN (mitochondria ER length indicator nanosensor). Here, we describe the rationale behind the MERLIN biosensor, detail the experimental setup and methodology, and provide tips for troubleshooting.作者: Fabric 時間: 2025-3-23 07:24
Janette V. Moreno,Jennifer Rangelthe UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.作者: GNAT 時間: 2025-3-23 11:55 作者: Atheroma 時間: 2025-3-23 16:57 作者: 壓艙物 時間: 2025-3-23 19:08
Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Ithe UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.作者: 捐助 時間: 2025-3-23 22:39 作者: ARBOR 時間: 2025-3-24 04:45 作者: Promotion 時間: 2025-3-24 06:54 作者: 大門在匯總 時間: 2025-3-24 11:24
Single-Cell NanoBRET Imaging with Green-Range HaloTag Acceptorapplications, mainly due to the low signal output resulting in poor signal-to-noise ratio. In this chapter, we describe a protocol to optimize spatiotemporal BRET imaging by adopting fluorescent HaloTag acceptors, adapting cell culture conditions and microscopic setup.作者: dragon 時間: 2025-3-24 15:01
Book 2022Latest editionund the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respecti作者: cogent 時間: 2025-3-24 19:37 作者: Talkative 時間: 2025-3-25 01:21
https://doi.org/10.1007/978-3-531-91181-6the host cells. Although BLI has such superior properties over FLI, the available wavelengths in BLI are mostly limited to the visible region. Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a quantum dot (QD) and luciferase–protein conjugate.作者: 遺產(chǎn) 時間: 2025-3-25 06:22 作者: Anticoagulant 時間: 2025-3-25 10:28 作者: 不容置疑 時間: 2025-3-25 14:41
Book 2022Latest editioniding known pitfalls.?.Authoritative and comprehensive, .Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2. presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories..作者: arthroplasty 時間: 2025-3-25 17:42 作者: Blasphemy 時間: 2025-3-25 21:53
https://doi.org/10.1007/978-3-531-91181-6selective and sensitive to all-.-RA (at-RA) in animal cells. This protocol exemplifies quantitative imaging of the RA levels in serum and cerebrospinal fluid with a linear range in two orders. The present protocol is an important addition to conventional techniques on quantitative imaging of endogenous at-RA levels in live mammalian cells.作者: REIGN 時間: 2025-3-26 01:48
https://doi.org/10.1007/978-3-658-16584-0 In the present chapter, we provide detailed protocols for setting up the NanoBiT-based homogenous binding assay using growth hormone secretagogue receptor type 1a (GHSR1a) and its endogenous agonist and antagonist as a representative model system.作者: induct 時間: 2025-3-26 05:29 作者: CLAN 時間: 2025-3-26 09:10 作者: ATRIA 時間: 2025-3-26 13:36 作者: Antarctic 時間: 2025-3-26 18:50
https://doi.org/10.1007/978-1-0716-2473-9Chemiluminescence; Molecular imaging; Heterogeneous conjugates; Protein fragments; BRET-based imaging; Bi作者: OATH 時間: 2025-3-27 01:02
978-1-0716-2475-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 大方不好 時間: 2025-3-27 04:51 作者: 語源學 時間: 2025-3-27 06:59
Sung-Bae KimIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Magnificent 時間: 2025-3-27 10:16 作者: rectocele 時間: 2025-3-27 15:36
https://doi.org/10.1007/978-3-531-91181-6ght to induce rapid immunogenic cell death with minimal off-target effects. In preclinical settings, bioluminescence imaging (BLI) is useful to quantitatively assess the efficacy of NIR-PIT for both in vitro and in vivo experiments, especially in the early phase of testing. Here, we describe the det作者: Flagging 時間: 2025-3-27 21:19
https://doi.org/10.1007/978-3-531-91181-6des. Metastasis of advanced HNSCC nearly always occurs first in neck lymph nodes before the development of distant metastasis. However, the development of preclinical animal models and therapeutic treatments for metastatic HNSCC is lagged from bench to clinic. In this protocol, we exemplify an ortho作者: 谷物 時間: 2025-3-27 23:12 作者: 饑荒 時間: 2025-3-28 04:31
https://doi.org/10.1007/978-3-531-91181-6, TiO., and CuO are synthesized via the sol-gel method. Antibacterial tests are carried out against Gram-positive . and Gram-negative . bacteria using disk diffusion and bioluminescence. To perform antibacterial tests on thin films and to overcome bacterial strains recuperation on the supports, a ne作者: archetype 時間: 2025-3-28 06:45
https://doi.org/10.1007/978-3-531-91181-6rescence imaging (FLI), BLI has a superior advantage that the background signals resulting from autofluorescence are almost zero due to the unnecessity of external excitation. In addition, BLI can permit a long-term observation of living cells because BL results in very low photocytotoxicity toward 作者: intrigue 時間: 2025-3-28 12:24 作者: 容易做 時間: 2025-3-28 17:36 作者: 休戰(zhàn) 時間: 2025-3-28 18:50
https://doi.org/10.1007/978-3-531-91181-6monstrates how to image RA activities indirectly in mammalian cells with ligand-activatable single-chain bioluminescence (BL) probes. We introduce 13 different molecular designs for characterizing an efficient single-chain probe that quantitatively visualizes RA activities with significant sensitivi作者: 不利 時間: 2025-3-28 22:59 作者: obscurity 時間: 2025-3-29 04:28 作者: 漸變 時間: 2025-3-29 08:59
Clare Woolhouse,Laura J. Nicholsoninase cascades are of high importance. Our group has developed a?split-luciferase biosensor system to monitor the activity of the Hippo pathway, a kinase cascade that regulates a wide variety of cellular processes. We have applied this biosensor for high-throughput screening to discover novel upstre作者: noxious 時間: 2025-3-29 11:29 作者: 貝雷帽 時間: 2025-3-29 16:25 作者: 完全 時間: 2025-3-29 20:37
Mentoring Diversity for Inclusion and Equitye of calcium and lipids between both organelles, as well as in apoptosis and in autophagy signaling. Despite their importance, due to their dynamic and heterogeneous nature, we still lack understanding of the molecular composition, structure, and regulation of these structures. In this chapter, we i作者: Ethics 時間: 2025-3-30 03:51
Freida Hopkins Outlaw,Janet Jacksonom a bioluminescent donor (luciferases) to a fluorescent acceptor. Since bioluminescence is an intrinsic phenomenon, BRET excludes the need for external illumination and serves as a powerful alternative to fluorescence-based systems. However, BRET has not been widely adopted for single-cell imaging 作者: 西瓜 時間: 2025-3-30 04:52 作者: Intervention 時間: 2025-3-30 09:48 作者: 爵士樂 時間: 2025-3-30 13:55 作者: SHRIK 時間: 2025-3-30 19:48 作者: 受辱 時間: 2025-3-30 21:03 作者: Thyroiditis 時間: 2025-3-31 04:17
BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase rescence imaging (FLI), BLI has a superior advantage that the background signals resulting from autofluorescence are almost zero due to the unnecessity of external excitation. In addition, BLI can permit a long-term observation of living cells because BL results in very low photocytotoxicity toward 作者: 前面 時間: 2025-3-31 05:00 作者: 悲痛 時間: 2025-3-31 09:28 作者: 浸軟 時間: 2025-3-31 17:23 作者: 蹣跚 時間: 2025-3-31 18:56
Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Co of protein–protein interactions (PPIs). Sensitive and quantitative protein association-based techniques represent a versatile tool to probe the architecture of receptor complexes and receptor–ligand interactions and expand the drug discovery toolbox by facilitating high-throughput screening (HTS) a作者: Terminal 時間: 2025-4-1 00:51
