標(biāo)題: Titlebook: Biological Electron Microscopy; Theory, Techniques, Michael J. Dykstra,Laura E. Reuss Textbook 2003Latest edition Michael J. Dykstra 2003 [打印本頁] 作者: antihistamine 時間: 2025-3-21 18:06
書目名稱Biological Electron Microscopy影響因子(影響力)
書目名稱Biological Electron Microscopy影響因子(影響力)學(xué)科排名
書目名稱Biological Electron Microscopy網(wǎng)絡(luò)公開度
書目名稱Biological Electron Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Biological Electron Microscopy被引頻次
書目名稱Biological Electron Microscopy被引頻次學(xué)科排名
書目名稱Biological Electron Microscopy年度引用
書目名稱Biological Electron Microscopy年度引用學(xué)科排名
書目名稱Biological Electron Microscopy讀者反饋
書目名稱Biological Electron Microscopy讀者反饋學(xué)科排名
作者: 非秘密 時間: 2025-3-21 23:47 作者: 轉(zhuǎn)換 時間: 2025-3-22 01:42
Techniques, The technique was pioneered by ., . and has since become a Standard technique (.). The strength o f the Tokuyasu technique is that it employs minimal fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is qui作者: promote 時間: 2025-3-22 08:11 作者: Customary 時間: 2025-3-22 11:09 作者: Diatribe 時間: 2025-3-22 15:41 作者: Factual 時間: 2025-3-22 20:25
Cytochemistry,h reagent and label penetration of tissues and cells, and problems distinguishing reaction products from other cellular constituents. The aim of this chapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities assoc作者: Offstage 時間: 2025-3-22 21:42 作者: Genteel 時間: 2025-3-23 03:51 作者: Urgency 時間: 2025-3-23 07:21
Techniques,tages of colloidal gold techniques is that the gold particles cannot be confused with any other cellular constituents, unlike the products of previously discussed ferritin and peroxidase/DAB/osmium staining procedures.作者: intoxicate 時間: 2025-3-23 11:17 作者: Aesthete 時間: 2025-3-23 16:59 作者: sultry 時間: 2025-3-23 19:01
Techniques,duration of evaporation, temperature of electrodes during evaporation (amperage used), angle of evaporation, vacuum at the time of evaporation, material being evaporated, and the temperature of the stage holding the specimen or support being coated are all factors for the end-product desired. As sta作者: Meditate 時間: 2025-3-24 00:05 作者: 襲擊 時間: 2025-3-24 05:00
Vacuum Systems, and thus are lost to the image-forming process. In order to have a working understanding of the vacuum System, it is necessary to examine the different types of vacuum sensors, or gauges, and vacuum pumps utilized in electron microscopy. This is especially important because various gauges and pumps作者: 媒介 時間: 2025-3-24 07:37 作者: BLANC 時間: 2025-3-24 11:27
https://doi.org/10.1007/978-1-4419-9244-4Fixation; electron microscopy; microscopy; scanning electron microscope; transmission electron microscop作者: 磨碎 時間: 2025-3-24 18:21
978-1-4613-4856-6Michael J. Dykstra 2003作者: AGOG 時間: 2025-3-24 21:46
Eva Langeland,Hege Forbech Vinjeed. These requirements have resulted in the development of two basic instruments over the years: thermal- and mechanicaladvance ultramicrotomes. An excellent and concise source of information on ultramicrotomy is the booklet “Ultramicrotomy: Frequent Faults and Problems” by (.). A more lengthy discussion of ultramicrotomy can be found in (.) book作者: agitate 時間: 2025-3-25 00:06 作者: 攀登 時間: 2025-3-25 06:10
https://doi.org/10.1007/978-1-59259-326-2. Dupouy in 1960 (.). In 1969, Dupouy installed a 3,000-kV instrument, which offered improved specimen penetration and reduced chromatic aberration, but did not offer significant advantages over the original 1,000-kV instrument.作者: Integrate 時間: 2025-3-25 07:49 作者: BOGUS 時間: 2025-3-25 14:39
https://doi.org/10.1007/978-94-007-5195-8ixation ideally instantaneously stops biological activity, immobilizes cellular components, and enables them to withstand any further processing procedures. In principle, biological materials can be fixed with either chemical or physical techniques (cold, heat). Good chemical fixation requires the f作者: 淡紫色花 時間: 2025-3-25 17:18
Eva Langeland,Hege Forbech Vinje The technique was pioneered by ., . and has since become a Standard technique (.). The strength o f the Tokuyasu technique is that it employs minimal fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is qui作者: 侵略主義 時間: 2025-3-25 21:33 作者: 不在灌木叢中 時間: 2025-3-26 03:19 作者: Lipoprotein(A) 時間: 2025-3-26 06:24
Mental Wellness Programs for Employeesre other resins such as the acrylic resins (Lowicryls, LR White and LR Gold) in common use that will have different staining responses from epoxides because of their partial miscibility with water. In addition, semithin frozen sections have still other staining characteristics resulting from their s作者: DEMN 時間: 2025-3-26 11:12
Mental Distress as a Problem for Industryh reagent and label penetration of tissues and cells, and problems distinguishing reaction products from other cellular constituents. The aim of this chapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities assoc作者: 吵鬧 時間: 2025-3-26 15:37 作者: obligation 時間: 2025-3-26 19:45 作者: 在駕駛 時間: 2025-3-26 21:41
Deutung und Diskussion der Ergebnisse,tages of colloidal gold techniques is that the gold particles cannot be confused with any other cellular constituents, unlike the products of previously discussed ferritin and peroxidase/DAB/osmium staining procedures.作者: 溫順 時間: 2025-3-27 04:37
Workload and Workload Measurementia, viruses). The films serve as electron-transparent Substrates for these samples. In addition, grids with large amounts of open space (bar, hole, or slot grids) that otherwise would provide inadequate support for plastic sections can be coated with films so that large areas of sections, or the ent作者: 性行為放縱者 時間: 2025-3-27 07:59 作者: Resistance 時間: 2025-3-27 12:55 作者: 公式 時間: 2025-3-27 14:18
Ann M. Graybiel PhD,Yasuo Kubota PhDelectron microscope (TEM). The scanning electron microscope involves circuitry that processes electronic Signals into an indirect image seen on a cathode ray tube or digital screen. All of the direct visualization methods involve light or electrons interacting with specimens and utilize either glass作者: Deference 時間: 2025-3-27 19:40
Assessing Cognition in Movement Disorders and thus are lost to the image-forming process. In order to have a working understanding of the vacuum System, it is necessary to examine the different types of vacuum sensors, or gauges, and vacuum pumps utilized in electron microscopy. This is especially important because various gauges and pumps作者: capsule 時間: 2025-3-28 00:16
https://doi.org/10.1007/978-1-59259-326-2. Dupouy in 1960 (.). In 1969, Dupouy installed a 3,000-kV instrument, which offered improved specimen penetration and reduced chromatic aberration, but did not offer significant advantages over the original 1,000-kV instrument.作者: 邊緣 時間: 2025-3-28 05:40 作者: Coronation 時間: 2025-3-28 08:33
Mental Distress as a Problem for IndustryThis procedure is used to stain 0.25- to 0.5-μm epoxide or acrylic resin sections for light microscopy and to impart contrast to the originally low-contrast semithin sections of these resin-embedded materials.作者: LEERY 時間: 2025-3-28 12:18
Workload and Workload MeasurementPolyvinyl formaldehyde resin (Formvar) is the most widely used support film material for coating grids because of its stability under the electron beam and the consistency of the films produced.作者: B-cell 時間: 2025-3-28 16:51 作者: myalgia 時間: 2025-3-28 20:33
Techniques,This procedure is used to stain 0.25- to 0.5-μm epoxide or acrylic resin sections for light microscopy and to impart contrast to the originally low-contrast semithin sections of these resin-embedded materials.作者: 啞劇 時間: 2025-3-29 02:48
Techniques,Polyvinyl formaldehyde resin (Formvar) is the most widely used support film material for coating grids because of its stability under the electron beam and the consistency of the films produced.作者: Hypopnea 時間: 2025-3-29 05:11
Michael J. Dykstra,Laura E. ReussCovers a wide range of topics including conventional light microscopy, transmission electron microscopy, and scanning electron microscopy.Includes a survey of each subject, and a techniques section, w作者: 平 時間: 2025-3-29 07:48 作者: 爭議的蘋果 時間: 2025-3-29 13:53
Ultramicrotomy,ed. These requirements have resulted in the development of two basic instruments over the years: thermal- and mechanicaladvance ultramicrotomes. An excellent and concise source of information on ultramicrotomy is the booklet “Ultramicrotomy: Frequent Faults and Problems” by (.). A more lengthy discussion of ultramicrotomy can be found in (.) book作者: Arboreal 時間: 2025-3-29 16:44
Techniques,tages of colloidal gold techniques is that the gold particles cannot be confused with any other cellular constituents, unlike the products of previously discussed ferritin and peroxidase/DAB/osmium staining procedures.作者: 可商量 時間: 2025-3-29 20:52 作者: 絕種 時間: 2025-3-30 03:56 作者: 經(jīng)典 時間: 2025-3-30 04:05
and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated 978-1-4613-4856-6978-1-4419-9244-4作者: 欲望 時間: 2025-3-30 11:48 作者: Deduct 時間: 2025-3-30 15:02
https://doi.org/10.1007/978-94-007-5195-8ontrast to chemical fixation, which consistently fixes samples to a depth of at least 0.5 mm. Cryofixation is often inferior for structural studies because of the limited sample size available but is vastly superior to chemical fixation for many microanalytical or immunocytochemical procedures becau作者: expeditious 時間: 2025-3-30 20:14 作者: Benign 時間: 2025-3-31 00:05
Staining Methods for Semithins and Ultrathins,ignificant hydrophilicity. For the purposes of this chapter, however, we will limit our discussion almost exclusively to the epoxide sections, since these are the most commonly used resins for biological work. Any of the stains for these resins will work with much reduced staining time on more water-miscible sections.作者: Junction 時間: 2025-3-31 02:54
Replicas, Shadowing, and Negative Staining,s, cells, and tissues with thin metal films that have areas of differential beam-stopping capability (replicas produced by shadowing). This chapter will discuss these two added techniques for building subtractive contrast, pointing out the commonly used techniques and a few remedies to specific problems that may be encountered.作者: compassion 時間: 2025-3-31 05:36 作者: 使人煩燥 時間: 2025-3-31 11:16 作者: ADORN 時間: 2025-3-31 15:07
Eva Langeland,Hege Forbech Vinje fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is quick and is one of the best ways to produce materials for consequent intracellular immunolabeling.作者: Nonflammable 時間: 2025-3-31 18:48
Mental Distress as a Problem for Industrychapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities associated with cellular surfaces and cytoplasmic contents.作者: EXUDE 時間: 2025-3-31 22:33 作者: 防御 時間: 2025-4-1 02:50 作者: 饑荒 時間: 2025-4-1 07:30
Techniques, fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is quick and is one of the best ways to produce materials for consequent intracellular immunolabeling.作者: 土坯 時間: 2025-4-1 12:00
Cytochemistry,chapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities associated with cellular surfaces and cytoplasmic contents.作者: NORM 時間: 2025-4-1 16:38 作者: 音樂等 時間: 2025-4-1 19:42
Vacuum Systems,nt types of vacuum sensors, or gauges, and vacuum pumps utilized in electron microscopy. This is especially important because various gauges and pumps can sustain damage if connected to the system being evacuated at an improper vacuum level.作者: AGGER 時間: 2025-4-2 01:00
Anne Kiefhaber,Willis B. Goldbeckor stick, as follows: heat dissecting needle tip to red hot with a Bunsen burner and . burn a small Channel in the tip of the applicator stick (Fig. 101C). Next, insert the end of the nichrome wire into the burned Channel and secure it by applying a small drop of 5-min epoxy to the assembly (Fig. 101D).
