標(biāo)題: Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2013Latest edition Springer Science+Business Media, New York 2013 Biologic [打印本頁(yè)] 作者: Malnutrition 時(shí)間: 2025-3-21 19:09
書目名稱Biological Aging影響因子(影響力)
書目名稱Biological Aging影響因子(影響力)學(xué)科排名
書目名稱Biological Aging網(wǎng)絡(luò)公開度
書目名稱Biological Aging網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Biological Aging被引頻次
書目名稱Biological Aging被引頻次學(xué)科排名
書目名稱Biological Aging年度引用
書目名稱Biological Aging年度引用學(xué)科排名
書目名稱Biological Aging讀者反饋
書目名稱Biological Aging讀者反饋學(xué)科排名
作者: 充滿人 時(shí)間: 2025-3-21 22:21
,Digital Image Analysis of Cells Stained with the Senescence-Associated β-Galactosidase Assay,an be used to identify senescent cells, of which the most widely used is the senescence-associated β-galactosidase (SABG) activity. Here we describe a procedure for digital image analysis of cells stained by the SABG staining technique at pH 6 or at pH 4. This analysis is highly reproducible and sen作者: Free-Radical 時(shí)間: 2025-3-22 01:59
Analysis of Biomarkers of Caloric Restriction in Aging Cells,wever, the accurate mechanisms, especially at the cellular level, for CR-induced aging delay are still under intense investigation. An emerging technique, recently explored in our laboratory, provides precisely controllable caloric intake in a cultured cellular system that allows real-time observati作者: archenemy 時(shí)間: 2025-3-22 07:50 作者: Commemorate 時(shí)間: 2025-3-22 10:43 作者: 定點(diǎn) 時(shí)間: 2025-3-22 14:43 作者: Asperity 時(shí)間: 2025-3-22 19:22 作者: 舊石器時(shí)代 時(shí)間: 2025-3-22 21:20
The Use of Calorie Restriction Mimetics to Study Aging, established experimental antiaging intervention. Several pharmacological agents that can replicate the beneficial effects of CR, called calorie restriction mimetics (CRMs), have been identified. The nutrient-sensing pathways including those involving sirtuins (especially SIRT1) and mammalian target作者: 金桌活畫面 時(shí)間: 2025-3-23 02:18 作者: 精美食品 時(shí)間: 2025-3-23 09:36 作者: 撕裂皮肉 時(shí)間: 2025-3-23 10:53
Methods of Cellular Senescence Induction Using Oxidative Stress, termed cell senescence. Human diploid fibroblasts offer a typical model for studying cell senescence in vitro. Various approaches to evoke oxidative stresses, such as the exposures of cells to ultraviolet light, ethanol, tert-butyl hydroperoxide (t-BHP), and peroxide hydrogen (H.O.), have been used作者: Dawdle 時(shí)間: 2025-3-23 13:58
Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice, includes the selection of mouse strain, sex, age at start of treatment, housing conditions, design of the long-term study, some noninvasive methods of assessment of biomarkers of aging, life-span parameters, pathology examination, and statistical treatment of the results.作者: 擔(dān)心 時(shí)間: 2025-3-23 21:12 作者: 娘娘腔 時(shí)間: 2025-3-23 23:44 作者: Enzyme 時(shí)間: 2025-3-24 03:22
Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging,ted by the large number of metabolites that are often detected. Overcoming this limitation requires high-resolution analytical separation techniques, coupled with high-power deconvolution software. Additionally, much care must be taken in metabolomic sample preparation to quench active enzymes and a作者: AMOR 時(shí)間: 2025-3-24 07:58
P.J.E. Bindels,C.M.F. Kneepkens as an important goal for gerontological research. As cellular senescence is characterized as the basis for aging in organisms, culturing and subculturing of normal human diploid fibroblasts to mimic the in vivo aging processes have been developed as major methods to investigate molecular events inv作者: 知識(shí)分子 時(shí)間: 2025-3-24 12:52 作者: 極端的正確性 時(shí)間: 2025-3-24 16:35 作者: 調(diào)味品 時(shí)間: 2025-3-24 22:14
Uwe Steffens,Ankatrin La?-Lentzschick and Moorhead in 1961, it has since become a popular model to study cellular aging. The replicative lifespan of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing with each population doubling (PD). Thus, the study of individual cells in作者: 自戀 時(shí)間: 2025-3-25 01:28 作者: Mortal 時(shí)間: 2025-3-25 06:38 作者: rectum 時(shí)間: 2025-3-25 10:18
https://doi.org/10.1007/978-3-642-47596-2describe methods commonly used in . aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of . in aging, such 作者: Fillet,Filet 時(shí)間: 2025-3-25 13:41 作者: inchoate 時(shí)間: 2025-3-25 15:48
,Ern?hrung und Ern?hrungsst?rungen,generated unique diploid genome is doomed with the individual’s inevitable demise. Since it was first reported in 1997 that Dolly the sheep had been cloned, many mammalian species have been cloned successfully using somatic-cell nuclear transfer (SCNT). The success of SCNT in mammals enables us not 作者: Conserve 時(shí)間: 2025-3-25 20:01
,Ern?hrung und Ern?hrungsst?rungen,ive genes that promote survival and proliferation of cancer cells. Paradoxically, introduction of an activated oncogene leads to activation of a state similar to cellular senescence, which is referred to as oncogene-induced senescence. Studies have shown that oncogene-induced senescence function as 作者: 頌揚(yáng)本人 時(shí)間: 2025-3-26 00:28 作者: 高腳酒杯 時(shí)間: 2025-3-26 06:25 作者: 包庇 時(shí)間: 2025-3-26 10:49 作者: 劇毒 時(shí)間: 2025-3-26 15:42
Herz- und Kreislauferkrankungen, understand the healing process. Using transgenic mice coupled with gene-modified cells, one can employ this method to monitor host and graft activity in various models of tissue regeneration. We implemented real-time bioluminescence functional imaging to analyze bone formation by following a unique作者: 合唱團(tuán) 時(shí)間: 2025-3-26 18:58
Erkrankungen des Verdauungstraktes,ted by the large number of metabolites that are often detected. Overcoming this limitation requires high-resolution analytical separation techniques, coupled with high-power deconvolution software. Additionally, much care must be taken in metabolomic sample preparation to quench active enzymes and a作者: 休閑 時(shí)間: 2025-3-26 22:14 作者: 歌曲 時(shí)間: 2025-3-27 03:47
978-1-4939-6313-3Springer Science+Business Media, New York 2013作者: craving 時(shí)間: 2025-3-27 06:35
Trygve O. TollefsbolFocuses on contemporary approaches and established protocols to study aging cell culture.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr作者: AWE 時(shí)間: 2025-3-27 12:32 作者: 群島 時(shí)間: 2025-3-27 16:11 作者: 圍巾 時(shí)間: 2025-3-27 19:11 作者: 并入 時(shí)間: 2025-3-28 00:35 作者: 變態(tài) 時(shí)間: 2025-3-28 03:39
Aging Studies in Drosophila Melanogaster,describe methods commonly used in . aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of . in aging, such as stress resistance and locomotor activity.作者: 小官 時(shí)間: 2025-3-28 09:35
Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice, includes the selection of mouse strain, sex, age at start of treatment, housing conditions, design of the long-term study, some noninvasive methods of assessment of biomarkers of aging, life-span parameters, pathology examination, and statistical treatment of the results.作者: 不愛防注射 時(shí)間: 2025-3-28 11:27
Uwe Steffens,Ankatrin La?-Lentzsch separation of cells based on different characteristics which can be measured by flow cytometry. Here, we describe various methods by which senescent cells can be sorted from mixed cultures and discuss how different methods impact on the posterior analysis of sorted populations.作者: acrophobia 時(shí)間: 2025-3-28 18:27
https://doi.org/10.1007/978-3-642-47596-2 using animal models. In this chapter, we focus on candidate CRMs including sirtuin-activating compounds (STACs) and mTOR inhibitors, their slowing of aging, and methods for evaluation of lifespan and metabolic disorders.作者: Collected 時(shí)間: 2025-3-28 22:22 作者: 中世紀(jì) 時(shí)間: 2025-3-29 00:44 作者: Chandelier 時(shí)間: 2025-3-29 04:12 作者: HIKE 時(shí)間: 2025-3-29 08:51 作者: 相一致 時(shí)間: 2025-3-29 11:28
Uwe Steffens,Ankatrin La?-Lentzschoach in .. A mutant selected from such a population is stabilized to recover a pure homozygous strain. In this chapter, we categorize the protocol into mutagenesis, phenotype screen, and outcross and provide time-tested procedures for their implementation to create long-lived worm mutants.作者: PHONE 時(shí)間: 2025-3-29 16:10 作者: LAVA 時(shí)間: 2025-3-29 20:16 作者: 修正案 時(shí)間: 2025-3-30 01:13 作者: Generic-Drug 時(shí)間: 2025-3-30 04:32
Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors,we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.作者: BLUSH 時(shí)間: 2025-3-30 09:43 作者: Irascible 時(shí)間: 2025-3-30 15:05 作者: enormous 時(shí)間: 2025-3-30 18:57 作者: Discrete 時(shí)間: 2025-3-30 21:00
Induction of Cellular Senescence by Oncogenic RAS,a barrier to neoplastic transformation. Here, we describe a protocol for inducing cellular senescence by oncogenic RAS in primary human fibroblast. The approach that we describe here is of general utility and can be applied to study senescence induction by other defined genetic elements, for example, other activated oncogenes.作者: 生來(lái) 時(shí)間: 2025-3-31 04:28 作者: 通知 時(shí)間: 2025-3-31 05:10 作者: Hamper 時(shí)間: 2025-3-31 10:39 作者: 才能 時(shí)間: 2025-3-31 13:26 作者: Frequency-Range 時(shí)間: 2025-3-31 19:57
Erkrankungen des Verdauungstraktes,d using the nematode ., an organism which has been widely exploited in aging studies; however, with only minimal modification, our technique is extendible to other sample types, and indeed we have successfully used it both to perform yeast footprinting and to study the excreted metabolic end products of human kidney cancer cell lines.作者: GROVE 時(shí)間: 2025-3-31 22:25
Analysis of Biomarkers of Caloric Restriction in Aging Cells,rtant clinical implications for future preventive approaches for aging and aging-related degeneration diseases in humans. Hence, we will discuss the detailed procedures of this novel technique as well as the analysis of relevant aging biomarkers and its broad application in the field.作者: strain 時(shí)間: 2025-4-1 04:19
