標題: Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2007 Humana Press 2007 DNA.Evolution.Microarray.Mutation.Proteomics.Telome [打印本頁] 作者: 遠見 時間: 2025-3-21 17:40
書目名稱Biological Aging影響因子(影響力)
書目名稱Biological Aging影響因子(影響力)學科排名
書目名稱Biological Aging網(wǎng)絡(luò)公開度
書目名稱Biological Aging網(wǎng)絡(luò)公開度學科排名
書目名稱Biological Aging被引頻次
書目名稱Biological Aging被引頻次學科排名
書目名稱Biological Aging年度引用
書目名稱Biological Aging年度引用學科排名
書目名稱Biological Aging讀者反饋
書目名稱Biological Aging讀者反饋學科排名
作者: bioavailability 時間: 2025-3-21 20:38 作者: 收到 時間: 2025-3-22 01:33
,Andere Leistungen für Kinder (§ 65 EStG),us, the unicellular . is a simple model system that can provide significant insights into the human genetics and molecular biology of aging. Here, we describe the standard procedures to measure the chronological life span, including both the normal and calorie restriction paradigms.作者: 散開 時間: 2025-3-22 06:08
,Andere Leistungen für Kinder (§ 65 EStG), methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in . (.–.).作者: 我怕被刺穿 時間: 2025-3-22 10:32
https://doi.org/10.1007/978-3-8349-9205-5steps in our bud-scar-sorting system are the use of small microbeads, which allows successive rounds of purification and regrowth of the mother cells (M-cell), and utilization of flow cytometry to sort and isolate cells with a longer lifespan based on the number of bud scars specifically labeled with WGA.作者: 不可侵犯 時間: 2025-3-22 13:29
E.G. Haarman,G.A. van Essen,A.M. van Furthicroarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.作者: 狗舍 時間: 2025-3-22 17:32
1064-3745 lifespan using high-throughput screening systems.Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protoco作者: APEX 時間: 2025-3-22 21:49 作者: notification 時間: 2025-3-23 04:23 作者: 有限 時間: 2025-3-23 06:54 作者: Ingratiate 時間: 2025-3-23 12:56 作者: lipoatrophy 時間: 2025-3-23 15:47
A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems,y from .-adenosyl-.-methionine (SAM) to the 5-position of cytosines in the CpG dinucleotide. Several studies illustrate changes in activity and transcription of DNMT1 during aging and here we show a comprehensive method of detection of DNMT1 mRNA transcription from senescing cells in culture.作者: Magisterial 時間: 2025-3-23 19:32
Caloric Restriction Mimetics,aging effects of CR without the need to reduce food intake significantly. To date, a number of candidate CR mimetics including glycolytic inhibitors, antioxidants and specific gene-modulators have been investigated and appear to validate the potential of this approach.作者: 使痛苦 時間: 2025-3-24 02:06
Methods of Cellular Senescence Induction Using Oxidative Stress,rogen peroxide is widely used to achieve oxidative stress-induced premature senescence within a short period of time. Such induced premature senescent cells display many markers that are indistinguishable from replicative senescent cells. Thus, oxidative stress-induced senescent cells serve as an excellent in vitro tool for aging research.作者: Tidious 時間: 2025-3-24 04:58
Nuclear Transfer Methods to Study Aging,r embryos. This protocol first focuses on use of nuclear transfer to study nucleus versus cytoplasmic origin in agingassociated meiosis defects in oocytes at the germinal vesicle (GV) stage. Then, nuclear transfer is used at the zygote stage to study nuclear and cytoplasmic abnormality and apoptosis in early development.作者: HERE 時間: 2025-3-24 08:51
Book 2007to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging. .作者: 極力證明 時間: 2025-3-24 11:16 作者: Fillet,Filet 時間: 2025-3-24 17:16 作者: Flustered 時間: 2025-3-24 20:03 作者: 徹底明白 時間: 2025-3-24 23:52
The Chronological Life Span of ,,us, the unicellular . is a simple model system that can provide significant insights into the human genetics and molecular biology of aging. Here, we describe the standard procedures to measure the chronological life span, including both the normal and calorie restriction paradigms.作者: Fulsome 時間: 2025-3-25 03:27
Caloric Restriction and Life Span Determination of Yeast Cells, methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in . (.–.).作者: JUST 時間: 2025-3-25 11:06
Identifying Genes that Extend Life Span Using a High-Throughput Screening System,steps in our bud-scar-sorting system are the use of small microbeads, which allows successive rounds of purification and regrowth of the mother cells (M-cell), and utilization of flow cytometry to sort and isolate cells with a longer lifespan based on the number of bud scars specifically labeled with WGA.作者: Magisterial 時間: 2025-3-25 12:32
Application of DNA Microarray Technology to Gerontological Studies,icroarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.作者: 反復無常 時間: 2025-3-25 15:50
,Anspruchsberechtigte (§ 62 EStG),different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.作者: 極小 時間: 2025-3-25 23:24 作者: 水槽 時間: 2025-3-26 02:49
,Andere Leistungen für Kinder (§ 65 EStG),tein expression because studies illustrate that it is possible for the enzymes to undergo posttranslational physical changes in response to stimulation even though gene transcription remains unchanged. Here, we discuss an in vitro method to study protein expression of DNMTs in aging systems.作者: 減至最低 時間: 2025-3-26 04:46
Methods for Cell Sorting of Young and Senescent Cells,different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.作者: 小爭吵 時間: 2025-3-26 08:47
Telomere Length Analysis,on methods have improved greatly in accuracy and sensitivity since the development of the conventional telomeric Southern blot. Here, we describe the different methodologies recently developed for telomere length quantification, as well as their potential applications for human aging studies.作者: 吝嗇性 時間: 2025-3-26 13:17
A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro,tein expression because studies illustrate that it is possible for the enzymes to undergo posttranslational physical changes in response to stimulation even though gene transcription remains unchanged. Here, we discuss an in vitro method to study protein expression of DNMTs in aging systems.作者: 仔細閱讀 時間: 2025-3-26 20:15
https://doi.org/10.1007/978-1-59745-361-5DNA; Evolution; Microarray; Mutation; Proteomics; Telomere; Termination; age; gene transcription; genes; hybri作者: Grandstand 時間: 2025-3-26 23:26 作者: legitimate 時間: 2025-3-27 01:27 作者: Restenosis 時間: 2025-3-27 05:24
,Andere Leistungen für Kinder (§ 65 EStG),The methodology of testing anti-aging drugs in laboratory mice is presented. It includes the selection of mouse strain, sex, age at start of treatment, housing conditions, design of the long-term study, some noninvasive methods of assessment, pathology examination, and statistical treatment of the results.作者: 抑制 時間: 2025-3-27 10:18 作者: 鞭打 時間: 2025-3-27 17:33
Biological Aging978-1-59745-361-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: synovitis 時間: 2025-3-27 17:57 作者: 過度 時間: 2025-3-28 00:38
,Familienleistungsausgleich (§ 31 EStG),ess is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibroblasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have作者: 臥虎藏龍 時間: 2025-3-28 05:59
https://doi.org/10.1007/978-3-8349-9205-5ogenic stimuli, in culture and most likely in vivo. Cellular senescence is characterized by an irreversible growth arrest and certain altered functions. Senescent cells in culture are identified by their inability to undergo DNA synthesis, a property also shared by quiescent cells. Several years ago作者: Rejuvenate 時間: 2025-3-28 06:53
,Anspruchsberechtigte (§ 62 EStG), aging process. However, the replicative life span of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing at each population doubling, rather than all cells entering senescence simultaneously. Thus, the study of individual cells in a mass cu作者: cardiopulmonary 時間: 2025-3-28 12:11
,Andere Leistungen für Kinder (§ 65 EStG),sues, which has been related to alteration of tissue function and loss of regenerative capabilities in aging and aging-related diseases. Hence, telomere length is an important biomarker for aging and can be used in the prognosis of aging diseases. These facts highlight the importance of developing m作者: 不法行為 時間: 2025-3-28 16:01 作者: 漂白 時間: 2025-3-28 21:25 作者: 施加 時間: 2025-3-29 00:43 作者: 無政府主義者 時間: 2025-3-29 05:10 作者: 硬化 時間: 2025-3-29 11:02 作者: ETHER 時間: 2025-3-29 13:33 作者: Etymology 時間: 2025-3-29 18:14
,Anspruchsberechtigte (§ 62 EStG),ny human cell types is regulated by the length and status of telomeric sequences, which is shortened after each round of DNA replication. Telomeres can be rejuvenated by telomerase, an enzyme which carries out de novo synthesis of telomeric DNA. Telomerase is a ribonucleoprotein complex composed min作者: 詞匯表 時間: 2025-3-29 23:08
,übergangsregelungen (§ 78 EStG),Cellular senescence can be induced through several different mechanisms. Primary mammalian cells display a finite life span, suggesting a mechanism that counts cell divisions. Those cells initially proliferate but eventually enter a state of permanent growth arrest, called replicative senescence. Er作者: 裝入膠囊 時間: 2025-3-30 00:48
,übergangsregelungen (§ 78 EStG),, which is commonly known as replicative senescence. This led to the suggestion that cellular senescence might be a cellular basis of human aging. Indeed, cells with the characteristics of senescence accumulate with age in multiple tissues from both humans and rodents, thus implying a role of cellul作者: Fibrin 時間: 2025-3-30 05:41 作者: 水槽 時間: 2025-3-30 11:18 作者: Certainty 時間: 2025-3-30 14:50 作者: 角斗士 時間: 2025-3-30 19:32 作者: 悲痛 時間: 2025-3-30 21:00 作者: gentle 時間: 2025-3-31 03:10
https://doi.org/10.1007/978-3-8349-9205-5ells even in heterogeneous cell populations and aging tissues, such as skin biopsies from older individuals. Because it is easy to detect, SA-β-gal is currently a widely used biomarker of senescence. Here we describe a method to detect SA-β-gal in detail, including some recent modifications.作者: seroma 時間: 2025-3-31 05:20 作者: DOTE 時間: 2025-3-31 09:41 作者: 繞著哥哥問 時間: 2025-3-31 15:48
,übergangsregelungen (§ 78 EStG),rphological features. Thereby, p53 seems to be essential for the senescence response. Many of these senescence inducing mechanisms can be experimentally recapitulated by the introduction of defined genetic elements. Replicative senescence due to telomere shortening can, for example, be induced by a 作者: Androgen 時間: 2025-3-31 21:00 作者: critique 時間: 2025-4-1 01:09
Methods to Detect Biomarkers of Cellular Senescence,ells even in heterogeneous cell populations and aging tissues, such as skin biopsies from older individuals. Because it is easy to detect, SA-β-gal is currently a widely used biomarker of senescence. Here we describe a method to detect SA-β-gal in detail, including some recent modifications.作者: 狼群 時間: 2025-4-1 04:21 作者: intrigue 時間: 2025-4-1 08:03 作者: STEER 時間: 2025-4-1 12:30 作者: Trochlea 時間: 2025-4-1 17:42 作者: 取回 時間: 2025-4-1 20:39
Techniques for Analysis of Biological Aging,des, a popular mode of studying biological aging has been the analysis of cells cultured in vitro that display cellular senescence. Current interest is also focused on models of senescence that include organismal aging such as aging of yeast and .. The number of techniques applied to biological agin作者: 走調(diào) 時間: 2025-4-2 01:19
Aging Cell Culture,ess is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibroblasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have作者: 啜泣 時間: 2025-4-2 05:45
Methods to Detect Biomarkers of Cellular Senescence,ogenic stimuli, in culture and most likely in vivo. Cellular senescence is characterized by an irreversible growth arrest and certain altered functions. Senescent cells in culture are identified by their inability to undergo DNA synthesis, a property also shared by quiescent cells. Several years ago
