標(biāo)題: Titlebook: Biolistic DNA Delivery in Plants; Methods and Protocol Sachin Rustgi,Hong Luo Book 2020 Springer Science+Business Media, LLC, part of Sprin [打印本頁] 作者: purulent 時(shí)間: 2025-3-21 16:44
書目名稱Biolistic DNA Delivery in Plants影響因子(影響力)
書目名稱Biolistic DNA Delivery in Plants影響因子(影響力)學(xué)科排名
書目名稱Biolistic DNA Delivery in Plants網(wǎng)絡(luò)公開度
書目名稱Biolistic DNA Delivery in Plants網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Biolistic DNA Delivery in Plants被引頻次
書目名稱Biolistic DNA Delivery in Plants被引頻次學(xué)科排名
書目名稱Biolistic DNA Delivery in Plants年度引用
書目名稱Biolistic DNA Delivery in Plants年度引用學(xué)科排名
書目名稱Biolistic DNA Delivery in Plants讀者反饋
書目名稱Biolistic DNA Delivery in Plants讀者反饋學(xué)科排名
作者: 商業(yè)上 時(shí)間: 2025-3-21 22:21
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187279.jpg作者: Ringworm 時(shí)間: 2025-3-22 00:49 作者: Cervical-Spine 時(shí)間: 2025-3-22 05:02
https://doi.org/10.1007/978-3-322-85792-7bination also plays mechanical roles in mediating pairing of homologous chromosomes during prophase and in ensuring regular segregation of homologous pairs at the first meiotic division. The laboratory directed by D. von Wettstein identified six key steps in the meiotic process: (1) Recombination me作者: Cytokines 時(shí)間: 2025-3-22 12:30 作者: recession 時(shí)間: 2025-3-22 13:40 作者: 神圣不可 時(shí)間: 2025-3-22 20:09
,Mikrowelten: Brutk?sten für Wissen,not dependent on the cell type, species, and genotype. The success of any transformation technique depends on the starting experimental materials or the explants. Here, we describe the factors that have influenced the choice of explants in biolistic transformation. Many general factors in the select作者: Chipmunk 時(shí)間: 2025-3-23 00:08
,Gro?e Ideen in geistgerechten Bissen,roparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research. Here, we review the technical aspects and the major applications of the biolistic method for studies involving transient gene expression in plant cells. These studies cover multiple areas of pla作者: 不理會(huì) 時(shí)間: 2025-3-23 02:35
Kinder, Kindheiten, Konstruktionene relatively large micron-scale biolistic projectiles can result in tissue damage, low regeneration efficiency, and create difficulties for the biolistic transformation of isomorphic small cells or subcellular target organelles (i.e., mitochondria and plastids). As an alternative to micron-sized car作者: Circumscribe 時(shí)間: 2025-3-23 08:37
Doris Bühler-Niederberger,Heinz Sünker. One of the methodologies for genetic improvement is biolistic delivery of genetic components into plant cells. In this chapter, we describe steps involved in introducing plasmid DNA carrying gene of interest into rice mature embryos using Biolistic. PDS-1000/He particle delivery system. We also pr作者: Suppository 時(shí)間: 2025-3-23 13:03 作者: Gyrate 時(shí)間: 2025-3-23 15:47
https://doi.org/10.1007/978-3-531-90483-2y, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since th作者: Employee 時(shí)間: 2025-3-23 20:43 作者: Toxoid-Vaccines 時(shí)間: 2025-3-24 01:34
https://doi.org/10.1007/978-3-663-08069-5ted 12–16?days post-anthesis, the embryonic axis is removed, and the immature scutella are precultured for 1–2?days prior to particle bombardment. Gold particles are coated with plasmid DNA containing the gene(s) of interest plus a selectable marker gene, in this instance . (bialaphos resistance), a作者: patriarch 時(shí)間: 2025-3-24 05:15 作者: CONE 時(shí)間: 2025-3-24 09:16 作者: 可忽略 時(shí)間: 2025-3-24 12:56 作者: 任命 時(shí)間: 2025-3-24 14:59
https://doi.org/10.1007/978-3-531-90904-2ists, especially in the era of genome editing. The ability to deliver proteins or protein/RNA complexes into cells allows for producing gene-edited plants that are free of transgene integration in the genome. Here we describe a protocol for the delivery of a protein/gold particle mixture in plant ce作者: 睨視 時(shí)間: 2025-3-24 19:12 作者: PAEAN 時(shí)間: 2025-3-24 23:24 作者: 單獨(dú) 時(shí)間: 2025-3-25 04:33
978-1-0716-0358-1Springer Science+Business Media, LLC, part of Springer Nature 2020作者: 令人作嘔 時(shí)間: 2025-3-25 11:18
1064-3745 ation advice from the experts.This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, a作者: oxidant 時(shí)間: 2025-3-25 13:52 作者: wreathe 時(shí)間: 2025-3-25 18:02
Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seedssecting microscope and subjected to bombardment with plasmid DNA containing a bialaphos-resistance (.) gene. PCR, Southern, and Northern blot analyses are used to confirm the transgene integration and expression.作者: BATE 時(shí)間: 2025-3-25 22:26
Book 2020oubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Biolistic DNA Delivery: Methods and Protocols .aims to. .provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation..作者: 陶醉 時(shí)間: 2025-3-26 03:30 作者: 惡名聲 時(shí)間: 2025-3-26 04:57 作者: 卵石 時(shí)間: 2025-3-26 11:31
Jugendarbeit als Subjektbildung 6?months to obtain the viable transformants in the spring wheat background. In the present communication, we demonstrated the use of this method to produce the reduced immunogenicity wheat genotypes.作者: 連累 時(shí)間: 2025-3-26 15:17
Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applicationshis chapter. His life is an inspiration to the next generation of biologists. Despite difficult situations, his persistent efforts and keen desire to learn enabled him to overcome obstacles. He always tried to attain the best, excelling in translating fundamental knowledge into applications.作者: 禁止 時(shí)間: 2025-3-26 16:54
Biolistic DNA Delivery and Its Applications in l parameters of DNA delivery. In this chapter, the history, application, and current development of biolistic DNA delivery in sorghum are reviewed, and the prospect of sorghum genetic engineering is discussed.作者: GEN 時(shí)間: 2025-3-26 22:53
Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat 6?months to obtain the viable transformants in the spring wheat background. In the present communication, we demonstrated the use of this method to produce the reduced immunogenicity wheat genotypes.作者: 敘述 時(shí)間: 2025-3-27 04:37 作者: inquisitive 時(shí)間: 2025-3-27 08:44 作者: 許可 時(shí)間: 2025-3-27 11:49 作者: Torrid 時(shí)間: 2025-3-27 15:24
Bildung als p?dagogisches Anliegentroduction of undesirable plasmid derived genes, delivering low-copy transgenic events. In this chapter, we describe the method for efficient delivery of genes into sugarcane cells using a biolistic approach.作者: BARK 時(shí)間: 2025-3-27 21:01 作者: 藐視 時(shí)間: 2025-3-28 00:21 作者: Measured 時(shí)間: 2025-3-28 05:29 作者: NADIR 時(shí)間: 2025-3-28 07:54 作者: 易達(dá)到 時(shí)間: 2025-3-28 13:46
Biolistic DNA Delivery in Maize Immature Embryosryos of maize Hi II hybrid genotype, using Biolistic. PDS-1000/He particle delivery system. While we focus on the biolistic delivery process in the protocol presented here, we also provide step-by-step information required for successful regeneration of transgenic maize plants.作者: 撫育 時(shí)間: 2025-3-28 15:35 作者: 熔巖 時(shí)間: 2025-3-28 21:39 作者: 描繪 時(shí)間: 2025-3-28 23:49 作者: etiquette 時(shí)間: 2025-3-29 05:33
https://doi.org/10.1007/978-3-663-08069-5n of PPT (phosphinothricin tripeptide), the active ingredient of glufosinate-ammonium containing herbicides, to help select transformants. This updated method introduces selection earlier in the regeneration process which provides a shortened protocol while maintaining high transformation efficiencies.作者: 酷熱 時(shí)間: 2025-3-29 07:51
Biolistic Transformation of Japonica Rice Varietiesical 50–70 putative independent transgenic callus lines can be generated from 100 bombarded embryos. Transgenic rice plantlets can be produced within 2?months after the initiation of seed germination for transformation.作者: 嬰兒 時(shí)間: 2025-3-29 14:04
Genetic Transformation of Common Wheat (, L.) Using Biolisticsn of PPT (phosphinothricin tripeptide), the active ingredient of glufosinate-ammonium containing herbicides, to help select transformants. This updated method introduces selection earlier in the regeneration process which provides a shortened protocol while maintaining high transformation efficiencies.作者: Airtight 時(shí)間: 2025-3-29 17:59
Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applicationso the development of science and education. His contributions spanned various fields of science such as genetics, physiology, ultrastructural analysis, molecular biology, genomics, and biotechnology including genome editing. He performed and promoted pioneering research in the fields of epigenetics,作者: 逢迎白雪 時(shí)間: 2025-3-29 20:27
Diter von Wettstein and The Meiotic Program of Pairing and Recombinationbination also plays mechanical roles in mediating pairing of homologous chromosomes during prophase and in ensuring regular segregation of homologous pairs at the first meiotic division. The laboratory directed by D. von Wettstein identified six key steps in the meiotic process: (1) Recombination me作者: 不滿分子 時(shí)間: 2025-3-30 01:54
A Short History and Perspectives on Plant Genetic Transformationinto plant biology but also started a new era in crop improvement and commercial farming. However, for many crop plants, efficient transformation and regeneration still remain a challenge even after more than 30?years of technical developments in this field. Recently, .I endonuclease-based genome ed作者: 遺留之物 時(shí)間: 2025-3-30 05:28
Choice of the Promoter for Tissue and Developmental Stage-Specific Gene Expression of genes of interest, change metabolic and regulatory pathways, or result in accumulation of valuable proteins or metabolites. However, to be efficient in targeted genetic modification, the chimeric gene construct should be designed properly. In particular, the promoters used to control transgene e作者: 生氣的邊緣 時(shí)間: 2025-3-30 10:23
Choice of Explant for Plant Genetic Transformationnot dependent on the cell type, species, and genotype. The success of any transformation technique depends on the starting experimental materials or the explants. Here, we describe the factors that have influenced the choice of explants in biolistic transformation. Many general factors in the select作者: 碎石頭 時(shí)間: 2025-3-30 15:41 作者: Dorsal 時(shí)間: 2025-3-30 17:37
Nanobiolistics: An Emerging Genetic Transformation Approache relatively large micron-scale biolistic projectiles can result in tissue damage, low regeneration efficiency, and create difficulties for the biolistic transformation of isomorphic small cells or subcellular target organelles (i.e., mitochondria and plastids). As an alternative to micron-sized car作者: Audiometry 時(shí)間: 2025-3-31 00:45
Biolistic Transformation of Japonica Rice Varieties. One of the methodologies for genetic improvement is biolistic delivery of genetic components into plant cells. In this chapter, we describe steps involved in introducing plasmid DNA carrying gene of interest into rice mature embryos using Biolistic. PDS-1000/He particle delivery system. We also pr作者: 矛盾心理 時(shí)間: 2025-3-31 03:53 作者: HUMP 時(shí)間: 2025-3-31 05:31
Biolistic DNA Delivery and Its Applications in y, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since th
