作者: 矛盾 時間: 2025-3-21 22:10
Characterization of Endothelial Internalization and Targeting of Antibody—Enzyme Conjugates in Cell es and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting. This chapter describes methodologies for evaluating the binding and internalization of labeled conjugates directed to endothelial surface adhesion molecules in c作者: 擦掉 時間: 2025-3-22 01:43 作者: rheumatism 時間: 2025-3-22 07:31 作者: intolerance 時間: 2025-3-22 10:19 作者: ovation 時間: 2025-3-22 14:31 作者: indicate 時間: 2025-3-22 19:39
Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminaseloitation of the catalytic properties of the microbial transglutaminase from . species (MTGase), that is, acyl transfer between γ-carboxamide groups and various primary amines, new techniques for the enzymatic modification of proteins were developed. One example of bioconjugation is the biotinylatio作者: Sarcoma 時間: 2025-3-22 22:59
Fluorescent Sample Labeling for DNA Microarray Analysesrom total ribonucleic acid (RNA) samples. Two procedures are based on first-strand complementary DNA synthesis by reverse transcription. Label is introduced either by direct incorporation of fluorescently labeled nucleotides or indirectly by incorporation of aminoally-dUTP and subsequent coupling of作者: GORGE 時間: 2025-3-23 04:28
High-Density Labeling of DNA for Single Molecule Sequencingtarget into complementary flurophore-labeled DNA, and an exonuclease for the successive hydrolysis of the completely dye-labeled DNA. Recently, we found that the wild-type Klenow fragment of . DNA polymerase I is well-suited for the synthesis of DNA in a reaction set-up that contains exclusively spe作者: CRAFT 時間: 2025-3-23 07:03 作者: Lime石灰 時間: 2025-3-23 10:24 作者: Thymus 時間: 2025-3-23 14:15 作者: 障礙 時間: 2025-3-23 18:55 作者: incredulity 時間: 2025-3-24 01:24
Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodiesndothelial cells by anti-platelet endothelial cell adhesion molecule antibodies. However because of the modular nature of the streptavidin-biotin crosslinker system, the techniques herein can be easily adapted for the preparation of nanoscale immunoconjugates delivering other protein drugs to divers作者: 600 時間: 2025-3-24 04:45
Smart Polymer-Streptavidin Conjugatesded coil and a more hydrophobic, collapsed state that is smaller in volume. The differences in the polymer steric properties serve to block or allow ligand access to the binding site. The control of polymer molecular weight is a particularly important design parameter for these molecular gates.作者: arthroscopy 時間: 2025-3-24 08:45
Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties a functionally important amino group in the antigen-binding region of an antibody causes impairment or loss of the antigen binding function, resulting in a defective antibody conjugate that lacks one of its component functions. Furthermore, even if the chemical derivatization does not affect the an作者: CRANK 時間: 2025-3-24 11:02 作者: A保存的 時間: 2025-3-24 18:08
Sequence-Specific DNA Labeling Using Methyltransferasesically coupled with DNA in a DNA MTase-catalyzed reaction. By attaching interesting reporter groups to a suitable position of .-adenosylaziridine a large variety of new synthetic cofactors are obtained for sequence-specific labeling of DNA. This method is illustrated by coupling primary amino groups作者: Protein 時間: 2025-3-24 20:55
1064-3745 ion and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical dia978-1-59259-813-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Repetitions 時間: 2025-3-25 03:13 作者: figure 時間: 2025-3-25 03:40 作者: Synovial-Fluid 時間: 2025-3-25 10:38
Erratum zu: Krebs rechtzeitig erkennen,ded coil and a more hydrophobic, collapsed state that is smaller in volume. The differences in the polymer steric properties serve to block or allow ligand access to the binding site. The control of polymer molecular weight is a particularly important design parameter for these molecular gates.作者: 捏造 時間: 2025-3-25 13:26 作者: Legion 時間: 2025-3-25 17:57
https://doi.org/10.1007/978-3-662-59277-9ethod is thus particularly suitable when only a few biotin molecules need to be attached. Another example for the modification of proteins is the synthesis of hapten-protein conjugates used in competitive-type immunoassays. Methods for the synthesis of 2,4-.-casein conjugates (2,4-diclorophenoxyacet作者: Erythropoietin 時間: 2025-3-25 20:58 作者: 禮節(jié) 時間: 2025-3-26 03:15
R. Süss,V. Kinzel,J. D. Scribner Ph. D.ntibody-enzyme conjugates, which is controlled by the extent of biotinylation and molar ratio between the conjugate components, represents an important parameter that in some cases dictates subcellular addressing of drugs. This chapter describes the methodology of formation and characterization of p作者: insightful 時間: 2025-3-26 04:33
Wirtsfaktoren bei der Tumorentstehung,es and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting. This chapter describes methodologies for evaluating the binding and internalization of labeled conjugates directed to endothelial surface adhesion molecules in c作者: Urea508 時間: 2025-3-26 10:45 作者: 細(xì)菌等 時間: 2025-3-26 15:38 作者: 羊齒 時間: 2025-3-26 19:32 作者: 艦旗 時間: 2025-3-26 23:20 作者: 赤字 時間: 2025-3-27 03:53
https://doi.org/10.1007/978-3-662-59277-9loitation of the catalytic properties of the microbial transglutaminase from . species (MTGase), that is, acyl transfer between γ-carboxamide groups and various primary amines, new techniques for the enzymatic modification of proteins were developed. One example of bioconjugation is the biotinylatio作者: calumniate 時間: 2025-3-27 08:29 作者: ineluctable 時間: 2025-3-27 09:54 作者: 四目在模仿 時間: 2025-3-27 14:51 作者: 我要威脅 時間: 2025-3-27 18:22 作者: 公式 時間: 2025-3-27 22:31 作者: 枯萎將要 時間: 2025-3-28 04:09
Walter Felix Jungi,Hans-J?rg Sennotide-peptide and oligonucleotide-protein conjugates are described. These hybrid molecules can be used deliver chemical functionality to specific sequences within deoxyribonucleic acid and ribonucleic acid. The peptides and proteins can also be used to enhance the hybridization properties of the att作者: Overstate 時間: 2025-3-28 07:23 作者: cortisol 時間: 2025-3-28 14:17 作者: finite 時間: 2025-3-28 14:45
Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugatesotide-peptide and oligonucleotide-protein conjugates are described. These hybrid molecules can be used deliver chemical functionality to specific sequences within deoxyribonucleic acid and ribonucleic acid. The peptides and proteins can also be used to enhance the hybridization properties of the attached oligonucleotides.作者: 推延 時間: 2025-3-28 20:03 作者: animated 時間: 2025-3-29 02:24
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/186766.jpg作者: MODE 時間: 2025-3-29 04:25 作者: cajole 時間: 2025-3-29 07:30
Walter Felix Jungi,Hans-J?rg SennThis chapter describes the hydrophobic modification of peripheral membrane-anchored proteins by isoprenylation and .-acylation. The coupling of bacterially expressed protein moieties with chemically synthesized lipopeptides is described as an in vitro alternative for the generation of lipoproteins作者: RACE 時間: 2025-3-29 14:47
R. Obrist,D. P. Berger,J. P. ObrechtThis chapter describes general methodologies for the synthesis of lipidated, that is, prenylated and/or palmitoylated peptides. Standard operating procedures are given for peptide synthesis both on the polymeric support and in solution.作者: filial 時間: 2025-3-29 17:05 作者: Malcontent 時間: 2025-3-29 21:19 作者: 反饋 時間: 2025-3-30 02:35
Synthesis of Lipidated PeptidesThis chapter describes general methodologies for the synthesis of lipidated, that is, prenylated and/or palmitoylated peptides. Standard operating procedures are given for peptide synthesis both on the polymeric support and in solution.作者: Amorous 時間: 2025-3-30 04:15 作者: Heretical 時間: 2025-3-30 10:40
Immunologische Aspekte des Alterns, be adapted to the detection of other antibodies or antigens by exchanging the antigen-specific antibody. Several modifications of the method as well as optimization steps, potential error sources, and countermeasures are discussed.作者: 磨坊 時間: 2025-3-30 16:20
Characterization of Endothelial Internalization and Targeting of Antibody—Enzyme Conjugates in Cell adhesion molecule/tPA conjugates as an example. The experimental procedures, results, and notes provided may help in investigations of vascular immunotargeting of reporter, experimental, diagnostic, or therapeutic enzymes to endothelial and, perhaps, other cell types, both in vitro and in vivo.作者: tendinitis 時間: 2025-3-30 19:48 作者: PAEAN 時間: 2025-3-30 21:59 作者: etiquette 時間: 2025-3-31 03:42 作者: 不滿分子 時間: 2025-3-31 05:32 作者: BILK 時間: 2025-3-31 10:43
Erratum zu: Krebs rechtzeitig erkennen,e critically discussed on the basis of the properties of the protein to be modified and those desired for the final product. Methods for product purification and characterization are also provided. The overall information provided will guide the reader toward all of the critical steps involved in the preparation of PEG-protein adducts.作者: HUMID 時間: 2025-3-31 17:23 作者: Erythropoietin 時間: 2025-3-31 20:29
Conjugates of Peptides and Proteins to Polyethylene Glycolse critically discussed on the basis of the properties of the protein to be modified and those desired for the final product. Methods for product purification and characterization are also provided. The overall information provided will guide the reader toward all of the critical steps involved in the preparation of PEG-protein adducts.作者: Flavouring 時間: 2025-3-31 22:47 作者: 國家明智 時間: 2025-4-1 02:05
https://doi.org/10.1007/978-3-642-85368-5plementary DNA synthesis. While the first two methods are applied mainly in analyses on microarrays made from spotted polymerase chain reaction products or long oligonucleotides, the last procedure is mostly used for experiments on . synthesized oligonucleotide arrays.
