標(biāo)題: Titlebook: Bioconjugation; Methods and Protocol Sam Massa,Nick Devoogdt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 P [打印本頁] 作者: angiotensin-I 時間: 2025-3-21 19:32
書目名稱Bioconjugation影響因子(影響力)
書目名稱Bioconjugation影響因子(影響力)學(xué)科排名
書目名稱Bioconjugation網(wǎng)絡(luò)公開度
書目名稱Bioconjugation網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bioconjugation被引頻次
書目名稱Bioconjugation被引頻次學(xué)科排名
書目名稱Bioconjugation年度引用
書目名稱Bioconjugation年度引用學(xué)科排名
書目名稱Bioconjugation讀者反饋
書目名稱Bioconjugation讀者反饋學(xué)科排名
作者: 掃興 時間: 2025-3-21 20:54
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/186765.jpg作者: 毛細(xì)血管 時間: 2025-3-22 02:42 作者: confide 時間: 2025-3-22 07:45 作者: AUGER 時間: 2025-3-22 09:34
978-1-4939-9656-8Springer Science+Business Media, LLC, part of Springer Nature 2019作者: Influx 時間: 2025-3-22 13:30
https://doi.org/10.1007/978-3-531-18989-5ical ingredients (HPAPIs) selectively to tumor tissue. So far, HPAPIs have been mainly attached to the antibody via a chemical reaction of the payload with lysine or cysteine side chains of the antibody backbone. However, these conventional conjugation technologies result in formation of rather hete作者: Servile 時間: 2025-3-22 17:33 作者: Synapse 時間: 2025-3-23 00:54 作者: Affectation 時間: 2025-3-23 02:48
https://doi.org/10.1007/978-3-662-63117-1rmacological characteristics. We have developed a strategy to produce site-specific ADCs using a highly reactive lysine residue embedded in a dual-variable-domain (DVD) format. Here we provide protocols for the engineering, expression, and purification of the DVDs used for this strategy. We also pro作者: 商議 時間: 2025-3-23 08:41
,Künstliche Stimulanzien der Kreativit?t, drug-to-antibody ratios. One approach for generating homogeneous antibody conjugates utilizes enzymatic transfer of payloads onto a specific glycan or amino acid residue. Microbial transglutaminase (MTG) is an enzyme that catalyzes the formation of a stable isopeptide bond between a glutamine and a作者: Palate 時間: 2025-3-23 12:32 作者: Stable-Angina 時間: 2025-3-23 15:07
Teil 4: Ergebnisse des Theorietransfers,stics, and more. Most nonspecific chemical conjugation methods ligate onto any of a number of sites on the antibody, leading to multiple conjugated species, many of which perturb antibody function. To solve these problems, we used CRISPR/Cas9-edited hybridomas to introduce a Sortase tag (LPXTG) and 作者: 忘川河 時間: 2025-3-23 20:46 作者: Flawless 時間: 2025-3-23 22:35
https://doi.org/10.1007/978-3-658-31142-1ediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody–intein–chitin binding domain (CBD) fusion protein in . SHuffle. T7 cells, in the commonly used Luria–Bertani (LB) medium. The combination of these factors results in a high yield作者: watertight, 時間: 2025-3-24 02:59
https://doi.org/10.1007/978-3-658-31142-1y, pharmacokinetics, efficacy, and safety as well as improve manufacturing consistency. The SMARTag. technology platform offers a practical and efficient chemoenzymatic solution for site-specific protein modifications. A bioorthogonal aldehyde handle is introduced through the oxidation of a cysteine作者: conference 時間: 2025-3-24 08:15 作者: Angioplasty 時間: 2025-3-24 12:22
https://doi.org/10.1007/978-3-658-06581-2nzyme tubulin tyrosine ligase incorporates coumarin-amino acids to the terminal carboxylic acid of proteins containing a short peptidic recognition sequence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluoresce作者: CODE 時間: 2025-3-24 16:40
RaumFragen: Stadt – Region – Landschaftnstructs) largely due to facile library preparation and high throughput screening. Positron-emitting fluorine-18 (half-life?=?109.7?min) has a unique set of properties that match well with the pharmacokinetics of smaller sized constructs. Several indirect fluorine-18 labeling approaches have been de作者: 消息靈通 時間: 2025-3-24 20:07 作者: 發(fā)生 時間: 2025-3-25 00:49 作者: Nonporous 時間: 2025-3-25 06:40
Ergebnisse: Wirkungen im Umfeld, a powerful way to site-specifically introduce nonnatural chemical functionalities into recombinant proteins, which can be subsequently functionalized in a chemoselective manner. Even though several strategies currently exist to selectively label recombinant proteins in this manner, there is conside作者: 細(xì)胞膜 時間: 2025-3-25 08:10
,Problem – ein weiterer wichtiger Begriff,molecules for cell killing, or half-life extension modules for optimized pharmacokinetics. Although many conjugation strategies are straightforward to apply, most of them do not enable site-specific and orthogonal conjugation, and do not yield a defined stoichiometry. Moreover, techniques offering t作者: Medicaid 時間: 2025-3-25 12:06
Intelligenzforschung und Intelligenzmodelle,h, diagnostic and therapeutic applications. Many of these applications require the antibody to be labeled with additional chemical or biological moieties. Here, we describe a method for the rapid and site-specific labeling of nearly any “off-the-shelf” IgG. Our method utilizes small photoreactive an作者: BRAVE 時間: 2025-3-25 16:31
https://doi.org/10.1007/978-3-662-68927-1d architecture via conjugation. Telechelic proteins bearing the SpyTag and SpyCatcher reactive sequences can be expressed and purified for bioconjugation in vitro, giving protein conjugates, branched proteins, and circular proteins. By encoding both reactive sequences?in the same construct for expre作者: 偽善 時間: 2025-3-25 23:38
1064-3745 oubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Bioconjugation: Methods and Protocols. is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery..978-1-4939-9656-8978-1-4939-9654-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 自愛 時間: 2025-3-26 00:46 作者: 駕駛 時間: 2025-3-26 06:40
,Neurosen und psychosomatische St?rungen,acterial trans-peptidase Sortase A (Srt A). Srt A efficiently mediates sequence-specific peptide ligation under mild conditions and has few undesirable side reactions. We first describe the production, purification and characterization of Srt A enzyme and a scFv construct which targets activated pla作者: 有節(jié)制 時間: 2025-3-26 09:19 作者: 打折 時間: 2025-3-26 15:34 作者: 遺傳學(xué) 時間: 2025-3-26 20:13 作者: Harpoon 時間: 2025-3-26 21:09
Kreativit?t, Innovation, Entrepreneurship Here we present methods and protocols to express and label proteins of interest at the N-terminus with azide derivatives of myristic acid, a small, soluble, 14-carbon fatty acid, and conjugate the labeled protein to fluorophores and gold nanoparticle substrates. These methods can be extended to lab作者: 含鐵 時間: 2025-3-27 02:47
https://doi.org/10.1007/978-3-322-89806-7 C-terminal CaaX motif at the C-terminus of a protein substrate. The addition of only four amino acid residues minimizes the influence on the native protein structure. In addition, a variety of isoprenoid analogs containing different bioorthogonal functional groups, including azides, alkynes, and al作者: Polydipsia 時間: 2025-3-27 07:10
https://doi.org/10.1007/978-3-322-89806-7ties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload. These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-sp作者: offense 時間: 2025-3-27 10:29 作者: 祝賀 時間: 2025-3-27 17:35 作者: Nausea 時間: 2025-3-27 18:48
Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutamina a single lysine point mutation in an antibody to be efficiently transamidated. Here we describe the molecular positioning of these single engineered lysine residues and the conjugation conditions for producing homogeneous antibody conjugates exemplified using azido- and auristatin F-based acyl dono作者: floodgate 時間: 2025-3-28 00:06
Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine,acterial trans-peptidase Sortase A (Srt A). Srt A efficiently mediates sequence-specific peptide ligation under mild conditions and has few undesirable side reactions. We first describe the production, purification and characterization of Srt A enzyme and a scFv construct which targets activated pla作者: curriculum 時間: 2025-3-28 04:29
Site-Specific Modification of Proteins via Trypsiligase,ctionality exclusively at the desired position. We detail protocols for the expression and purification of trypsiligase as well as the construction of peptide or acyl donor ester probes used as substrates for the biocatalyst. In addition, we provide instructions how to perform the ultimate bioconjug作者: 昆蟲 時間: 2025-3-28 08:52
Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovativations that demand for homogeneously oriented nanobodies having their active site fully accessible for target (e.g., biomarker) binding, and (2) innovative applications such as localized drug delivery and image guided surgery by covalent “click” chemistry coupling of these alkynated nanobodies to a 作者: 繁重 時間: 2025-3-28 12:38 作者: 女歌星 時間: 2025-3-28 14:44 作者: VEIL 時間: 2025-3-28 21:59
Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransfe C-terminal CaaX motif at the C-terminus of a protein substrate. The addition of only four amino acid residues minimizes the influence on the native protein structure. In addition, a variety of isoprenoid analogs containing different bioorthogonal functional groups, including azides, alkynes, and al作者: geometrician 時間: 2025-3-29 02:53
,Homogeneous Antibody–Drug Conjugates via Glycoengineering,ties using sodium periodate provided an aldehyde handle on the glycans for conjugation with an aminooxy-assembled payload. These two strategies both enable gsADCs with high homogeneity in their conjugation sites, payload numbers, and glycoforms, which are characterized of a single mass under mass-sp作者: 鉆孔 時間: 2025-3-29 04:56
Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria,, a non-IgG binding scaffold, in a specific . strain to obtain high yields of protein carrying both a thiol and an azide group. We provide straightforward protocols for strain-promoted azide–alkyne cycloaddition (SPAAC) and thiol–maleimide conjugation, and furthermore compare these conjugation chemi作者: 熄滅 時間: 2025-3-29 08:43 作者: Apoptosis 時間: 2025-3-29 14:49 作者: 構(gòu)成 時間: 2025-3-29 16:52
https://doi.org/10.1007/978-3-658-06581-2quence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluorescently labeled proteins for advanced applications in imaging and diagnostics.作者: 思想上升 時間: 2025-3-29 21:40
,Engineering Dual Variable Domains for the Generation of Site-Specific Antibody–Drug Conjugates,iable-domain (DVD) format. Here we provide protocols for the engineering, expression, and purification of the DVDs used for this strategy. We also provide a protocol for DVD–drug conjugation and describe methods for their biochemical characterization, including a catalytic assay to monitor conjugation efficiency.作者: anthropologist 時間: 2025-3-30 03:58 作者: 人造 時間: 2025-3-30 07:02 作者: 浪費(fèi)時間 時間: 2025-3-30 12:10 作者: 形容詞詞尾 時間: 2025-3-30 15:02 作者: 繁重 時間: 2025-3-30 17:56 作者: 空氣傳播 時間: 2025-3-30 23:23 作者: arbiter 時間: 2025-3-31 04:11
RaumFragen: Stadt – Region – Landschaftite-specific method for coupling fluorine-18-labeled carboxylate substrates with biomolecules. Methods for preparation of two [.F]fluorocarboxylates and their ligation to a specific peptide sequence (LAP peptide) are presented herein.作者: 精密 時間: 2025-3-31 07:51
Ergebnisse: Wirkungen im Umfeld,ible with existing conjugation chemistries. Here we describe a method to express recombinant proteins in . site-specifically incorporating 5-hydroxytryptophan, followed by the chemoselective labeling of this residue using a chemoselective rapid azo-coupling reaction.作者: figment 時間: 2025-3-31 13:06 作者: commute 時間: 2025-3-31 17:09 作者: Paleontology 時間: 2025-3-31 20:30
CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow for Sie LPXTG is then acted on by the bacterial transpeptidase Sortase to site-specifically add on any of a number of chemical moieties that possess a triglycine repeat. This technique thus allows rapid production of an antibody onto which a wide array of chemical moieties can be site-specifically conjugated.作者: 忘川河 時間: 2025-3-31 22:31
Enzymatically Catalyzed Radiofluorination of Biomolecules,ite-specific method for coupling fluorine-18-labeled carboxylate substrates with biomolecules. Methods for preparation of two [.F]fluorocarboxylates and their ligation to a specific peptide sequence (LAP peptide) are presented herein.作者: 突變 時間: 2025-4-1 03:28
Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselecible with existing conjugation chemistries. Here we describe a method to express recombinant proteins in . site-specifically incorporating 5-hydroxytryptophan, followed by the chemoselective labeling of this residue using a chemoselective rapid azo-coupling reaction.
