標(biāo)題: Titlebook: Bifidobacteria; Methods and Protocol Douwe van Sinderen,Marco Ventura Book 2021 Springer Science+Business Media, LLC, part of Springer Natu [打印本頁(yè)] 作者: postpartum 時(shí)間: 2025-3-21 16:31
書(shū)目名稱Bifidobacteria影響因子(影響力)
作者: 奴才 時(shí)間: 2025-3-21 22:12
Transformation,ing selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we 作者: 漂白 時(shí)間: 2025-3-22 01:32 作者: INTER 時(shí)間: 2025-3-22 04:57 作者: 臭了生氣 時(shí)間: 2025-3-22 11:12
Site-Directed Mutagenesis of , Strains,jority of bifidobacterial strains represents a significant roadblock for the study of gene function and expression in these potential probiotics. Genetic tools for generating mutants are difficult to develop for bifidobacteria, as they require workarounds for obstacles such as low transformation eff作者: Exterior 時(shí)間: 2025-3-22 14:27 作者: 異常 時(shí)間: 2025-3-22 18:52 作者: MILK 時(shí)間: 2025-3-22 23:33 作者: COMMA 時(shí)間: 2025-3-23 02:05 作者: 浮夸 時(shí)間: 2025-3-23 05:32
Determination of Bifidobacterial Carbohydrate Utilization Abilities and Associated Metabolic End Prd polysaccharides. Different bifidobacterial strains may possess different carbohydrate utilization abilities. These metabolic abilities can be studied using classical bacterial growth assessment methods, such as measurement of changes in optical density or acidity of the culture in the presence of 作者: allergen 時(shí)間: 2025-3-23 12:40 作者: VEN 時(shí)間: 2025-3-23 16:44
Identification of Bifidobacteria by the Phosphoketolase Assay,g to their health and well-being. An important taxonomic marker for the identification of members of the bifidobacterial group is the presence of the fructose-6-phosphate phosphoketolase (F6PPK) activity. The F6PPK enzyme is involved in the bifidus shunt based on the ability of F6PPK to split fructo作者: Euthyroid 時(shí)間: 2025-3-23 19:37
Determination of Bile Salt Hydrolase Activity in Bifidobacteria,salt hydrolysis is considered to represent a bile detoxification mechanism for gut commensal bacteria and thus the presence of this activity was believed to be a predictor of bile tolerance of putative probiotic strains. On the other hand, it has recently been revealed that chemical modifications of作者: eardrum 時(shí)間: 2025-3-23 22:11 作者: 磨碎 時(shí)間: 2025-3-24 04:44
Metagenomic Analyses of Bifidobacterial Communities,s have been shown to be correlated with many aspects of host health by means of direct interactions with the host and cohabiting microbes. Metagenomic sequencing of fecal DNA represents a valuable approach for taxonomic and functional profiling of bacterial populations, and has allowed us to appreci作者: ambivalence 時(shí)間: 2025-3-24 08:00
Resistance of Bifidobacteria Toward Antibiotics,t safety record. However, in the context of the increasing worldwide problems associate to the rise of pathogenic microorganisms with acquired resistance to antibiotics, the risk associated to the presence of antibiotic resistance determinants should always be a key starting point for the introducti作者: Dorsal 時(shí)間: 2025-3-24 14:35 作者: Protein 時(shí)間: 2025-3-24 17:47 作者: 搜集 時(shí)間: 2025-3-24 19:54
Methods for Isolation and Recovery of Bifidobacteria, be encountered within the sample and the particular metabolic traits of the bifidobacterial species adapted to such inhabitation..A wide array of culture media recipes have been described in the literature to routinely isolate and grow bifidobacteria under laboratory conditions. However, there is n作者: Diaphragm 時(shí)間: 2025-3-25 01:49
Site-Directed Mutagenesis of , Strains, achieved using a nonreplicating vector, harboring a DNA fragment corresponding to an internal part of the gene to be mutated. This vector is introduced into a bifidobacterial cell of the strain in question by electroporation. Through homologous recombination, this vector is integrated into the geno作者: Certainty 時(shí)間: 2025-3-25 04:55 作者: 細(xì)微差別 時(shí)間: 2025-3-25 10:48
A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Ton) host. In this context, developing appropriate molecular tools to generate and investigate recombinant strains is necessary. While bifidobacteria have long remained recalcitrant to genetic manipulation, a wide array of .-specific replicating vectors and genetic modification procedures have been de作者: 肉身 時(shí)間: 2025-3-25 13:51
Resistance of Bifidobacteria Toward Antibiotics,tance pattern of the strain and the comparison with the susceptibility breakpoints for that species, allowing identifying the presence of atypical resistances in the strain. In this chapter we discuss the many efforts done to harmonize the methods used for the evaluation of antimicrobial susceptibil作者: 不愿 時(shí)間: 2025-3-25 18:47 作者: 設(shè)想 時(shí)間: 2025-3-25 21:38
https://doi.org/10.1007/978-3-642-66069-6 be encountered within the sample and the particular metabolic traits of the bifidobacterial species adapted to such inhabitation..A wide array of culture media recipes have been described in the literature to routinely isolate and grow bifidobacteria under laboratory conditions. However, there is n作者: attenuate 時(shí)間: 2025-3-26 00:11
Sex Reversal in the , (Polychaeta: Annelida) achieved using a nonreplicating vector, harboring a DNA fragment corresponding to an internal part of the gene to be mutated. This vector is introduced into a bifidobacterial cell of the strain in question by electroporation. Through homologous recombination, this vector is integrated into the geno作者: 搜集 時(shí)間: 2025-3-26 07:02
Kazuo Shirouzu MD, PhD,Yutaka Ogata MD, PhDe yet to be fully elucidated. Reports pertaining to CLAs have been attributed to suppressing tumor growth, delaying the onset of diabetes mellitus and reducing body fat in obese individuals. Given the increased attention for their bioactive properties, we describe in this chapter the qualitative and作者: peak-flow 時(shí)間: 2025-3-26 12:25 作者: 存心 時(shí)間: 2025-3-26 13:51
Polarization Profile near the 4430 Bandtance pattern of the strain and the comparison with the susceptibility breakpoints for that species, allowing identifying the presence of atypical resistances in the strain. In this chapter we discuss the many efforts done to harmonize the methods used for the evaluation of antimicrobial susceptibil作者: Amplify 時(shí)間: 2025-3-26 20:11
Kazuo Shirouzu MD, PhD,Yutaka Ogata MD, PhDorts to isolate novel and potentially beneficial strains. For this purpose, development of effective and selective isolation protocols in concert with knowledge on the physiological characteristics of bifidobacterial are fundamental requirements for their recovery and discovery from their natural environments, in particular from fecal samples.作者: seruting 時(shí)間: 2025-3-26 21:49 作者: 羞辱 時(shí)間: 2025-3-27 03:03
Protocol to Select Bifidobacteria from Fecal and Environmental Samples,orts to isolate novel and potentially beneficial strains. For this purpose, development of effective and selective isolation protocols in concert with knowledge on the physiological characteristics of bifidobacterial are fundamental requirements for their recovery and discovery from their natural environments, in particular from fecal samples.作者: CORD 時(shí)間: 2025-3-27 07:37 作者: 要素 時(shí)間: 2025-3-27 12:59 作者: Palter 時(shí)間: 2025-3-27 14:04
1064-3745 ation advice from the experts.This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial is作者: decipher 時(shí)間: 2025-3-27 19:17
The Sexuality of Pelecypod Molluscss. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 10.?transformants/μg of DNA.作者: START 時(shí)間: 2025-3-27 22:33 作者: 頭腦冷靜 時(shí)間: 2025-3-28 02:27 作者: ferment 時(shí)間: 2025-3-28 09:01
Dust Grains in Reflection Nebulae sequencing of fecal DNA represents a valuable approach for taxonomic and functional profiling of bacterial populations, and has allowed us to appreciate the relevance of bifidobacterial taxa in such complex bacterial communities, especially during the first stages of life.作者: Urgency 時(shí)間: 2025-3-28 11:26 作者: 性學(xué)院 時(shí)間: 2025-3-28 15:10
Isolation of Chromosomal and Plasmid DNA from Bifidobacteria,ted provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from . strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.作者: 毛細(xì)血管 時(shí)間: 2025-3-28 20:34 作者: 無(wú)彈性 時(shí)間: 2025-3-29 00:36
Metagenomic Analyses of Bifidobacterial Communities, sequencing of fecal DNA represents a valuable approach for taxonomic and functional profiling of bacterial populations, and has allowed us to appreciate the relevance of bifidobacterial taxa in such complex bacterial communities, especially during the first stages of life.作者: CHIP 時(shí)間: 2025-3-29 05:57 作者: Indelible 時(shí)間: 2025-3-29 07:30 作者: 羊齒 時(shí)間: 2025-3-29 12:09
Determination of Bifidobacterial Carbohydrate Utilization Abilities and Associated Metabolic End Pruid chromatography (HPLC) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) are extensively used in carbohydrate and metabolic end-product analysis due to their versatility and separation capabilities.作者: 秘方藥 時(shí)間: 2025-3-29 16:12 作者: 恩惠 時(shí)間: 2025-3-29 22:07
Measurements of Interstellar Extinctionization of SPF mice with human isolates of this genus through prior depletion of the gut resident microbiota with antibiotics. This chapter outlines the technical details for performing efficient microbiota depletion with antibiotics and subsequent administration of bifidobacteria for colonization.作者: invade 時(shí)間: 2025-3-30 03:00
Visible/UV Scattering by Interstellar Dustse-6-phosphate into erythrose-4-phosphate and acetyl phosphate. Here, we describe the two main methods utilized to detect the presence of F6PPK activity, that is, the enzymatic assay and the presence of the .-xylulose-5-phosphate/fructose-6-phosphate phosphoketolase bifidobacterial gene.作者: Astigmatism 時(shí)間: 2025-3-30 06:21
Polarization Profile near the 4430 Band used to predict active restriction–modification (RM) systems in . strains. The presence of active RM systems and knowledge on their target motifs may guide selection of suitable cloning or mutagenesis vectors for bifidobacteria.作者: 廣告 時(shí)間: 2025-3-30 11:30 作者: 四指套 時(shí)間: 2025-3-30 16:03 作者: photophobia 時(shí)間: 2025-3-30 19:31 作者: excrete 時(shí)間: 2025-3-30 22:47
Genome Assembly and Methylome Analysis Using Pacbio SMRT Sequencing, used to predict active restriction–modification (RM) systems in . strains. The presence of active RM systems and knowledge on their target motifs may guide selection of suitable cloning or mutagenesis vectors for bifidobacteria.作者: heterogeneous 時(shí)間: 2025-3-31 01:02 作者: 流浪 時(shí)間: 2025-3-31 05:17
Kazuo Shirouzu MD, PhD,Yutaka Ogata MD, PhDrt, by bacteriophage activity. Metagenomic studies of these populations is a crucial step in understanding this important interaction. This chapter outlines the technical instructions required to analyze the virome of a bifidobacteria-rich sample, for example, an infant fecal sample.作者: 我說(shuō)不重要 時(shí)間: 2025-3-31 12:05
