標(biāo)題: Titlebook: Beta-Arrestins; Methods and Protocol Mark G. H. Scott,Stéphane A. Laporte Book 2019 Springer Science+Business Media, LLC, part of Springer [打印本頁(yè)] 作者: Maculate 時(shí)間: 2025-3-21 19:17
書(shū)目名稱(chēng)Beta-Arrestins影響因子(影響力)
作者: Forehead-Lift 時(shí)間: 2025-3-21 22:59
Institutional Diversity in Bankingfic receptor activation occurred. In this arrangement PDEs acted as local “sinks” for cAMP and this enforced receptor-specific function by allowing the correct activation of a distinct pool of cAMP effectors in precise localizations. The discovery that β-arrestin could shuttle cAMP hydrolyzing activ作者: 躲債 時(shí)間: 2025-3-22 02:41 作者: dissolution 時(shí)間: 2025-3-22 07:54
Book 2019ctions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .Beta-Arrestins: Methods and Protocols .is a valuable resource for resear作者: 牽索 時(shí)間: 2025-3-22 08:59 作者: 秘方藥 時(shí)間: 2025-3-22 12:55 作者: 大方不好 時(shí)間: 2025-3-22 20:13 作者: 牌帶來(lái) 時(shí)間: 2025-3-22 21:50 作者: Optimum 時(shí)間: 2025-3-23 02:57
Mark G. H. Scott,Stéphane A. LaporteIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 沉著 時(shí)間: 2025-3-23 09:30
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/184350.jpg作者: Uncultured 時(shí)間: 2025-3-23 13:16 作者: MUTE 時(shí)間: 2025-3-23 15:47 作者: coddle 時(shí)間: 2025-3-23 22:05 作者: intimate 時(shí)間: 2025-3-23 23:35 作者: 殘忍 時(shí)間: 2025-3-24 03:37
Institutional Development PerformanceRs). They are now also known to act as multifunctional adaptor proteins binding many non-receptor protein partners to control multiple signalling pathways. β-arrs therefore act as key regulatory hubs at the crossroads of external cell inputs and functional outputs in cellular processes ranging from 作者: Progesterone 時(shí)間: 2025-3-24 07:01 作者: wreathe 時(shí)間: 2025-3-24 12:41 作者: Kernel 時(shí)間: 2025-3-24 16:11
Institutional Development Performanceegration of cellular signalling. We previously found that the angiotensin II (Ang?II) type 1 receptor (AT1R) and the prostaglandin F2α (PGF2α) receptor (FP), both important in the control of smooth muscle contractility, form such a functional heterodimeric complex in HEK 293 and vascular smooth musc作者: 標(biāo)準(zhǔn) 時(shí)間: 2025-3-24 21:54
Defining Institutional Development (ID) trafficking of internalized GPCRs to lysosomes. Ubiquitination of GPCRs is mediated by specific E3 ubiquitin ligases that are scaffolded by the adaptor proteins called β-arrestins. Traditionally, detection of GPCR ubiquitination is achieved by using ubiquitin antibodies to Western blot immunoprecip作者: AGOG 時(shí)間: 2025-3-25 01:59
https://doi.org/10.1007/978-981-13-7717-4proteins. Here we describe the methods used to identify the interaction sites of arrestin-binding partners on arrestin-3 and the use of monofunctional individual arrestin-3 elements in cells. Our in vitro pull-down assay with purified proteins demonstrates that relatively few elements in arrestin en作者: indifferent 時(shí)間: 2025-3-25 05:37 作者: 殺菌劑 時(shí)間: 2025-3-25 09:02
https://doi.org/10.1007/978-3-319-42073-8ed to stimulated G protein-coupled receptors (GPCRs), regulating their desensitization and internalization. The discovery that β-arrs could also?interact with more than 400 non-GPCR protein partners brought to light their central roles as multifunctional scaffold proteins regulating multiple signall作者: 保守黨 時(shí)間: 2025-3-25 13:33 作者: 多樣 時(shí)間: 2025-3-25 19:05
https://doi.org/10.1057/9780230620131teins can activate small G proteins both directly and indirectly. The activation of a variety of GPCRs leads to the translocation of Ral GDP dissociation stimulator (RalGDS) to the plasma membrane, where it functions as a guanine nucleotide exchange factor of RalA to promote membrane blebbing. The t作者: 相反放置 時(shí)間: 2025-3-25 23:40
Accessing Early-Stage Risk Capital in India,r biological function. The importance of this process in G protein-coupled receptor (GPCR) signalling has been fully demonstrated for many different receptors. For direct interactions, determining the interface regions, on β-arrestins and on the partners, is crucial for understanding the function of作者: CLAIM 時(shí)間: 2025-3-26 01:03
https://doi.org/10.1057/9780230620131k, in-depth exploration, and confrontation with kinetic biological data. Despite its standardization, dynamic modeling of signaling networks still requires successive technical steps that need to be carefully performed. Here, we detail these steps by going through the mathematical and statistical fr作者: –FER 時(shí)間: 2025-3-26 06:00
Dutch Rural Policies at a Turning Point,rotein–protein interactions in the past decades. In recent years, mass spectrometry (MS)-based proteomic methods have emerged as powerful tools to identify protein binding partners in a global and high-throughput manner. In this chapter, we describe the proteomic methods used to characterize the who作者: 輕信 時(shí)間: 2025-3-26 09:37
A Reconsideration of Holistic Economicsts, agonists may drive the receptor to preferentially engage one of these effectors over the other. Such “l(fā)igand bias” may present a means to impart pathway-selective signaling downstream of this class of receptors. In cases where physiological responses are mediated by diverse pathways, this could,作者: overture 時(shí)間: 2025-3-26 15:24 作者: 蝕刻 時(shí)間: 2025-3-26 19:27
Institutional Development Performance2 to AT1R and the AT1R/FP dimer in response to Ang II. Surprisingly, β-arrestin-1 and -2 were recruited to the dimer, in response to PGF2α as well, even though FP alone cannot recruit either β-arrestin-1 and -2.作者: OASIS 時(shí)間: 2025-3-26 22:51 作者: FECT 時(shí)間: 2025-3-27 03:58 作者: 洞察力 時(shí)間: 2025-3-27 08:43 作者: 束縛 時(shí)間: 2025-3-27 09:39 作者: enterprise 時(shí)間: 2025-3-27 17:22 作者: covert 時(shí)間: 2025-3-27 18:30
Proteomic Analysis of the β-Arrestin Interactomesestin signaling complexes. To investigate changes in the amounts of binding partners under different conditions, we also describe a SILAC (stable isotope labeling by amino acids in cell culture) method to obtain quantitative information for β-arrestin interactomes.作者: antecedence 時(shí)間: 2025-3-28 00:50
Defining Institutional Development (ID)ion, methodology has evolved accordingly over the last three decades since the discovery of the arrestin family. Herein we describe state-of-the-art approaches for studying the role of arrestins (β-arrestin1 . arrestin 2, β-arrestin2 . arrestin 3) in GPCR function in a primary cell type, cultured airway smooth muscle cells.作者: 音樂(lè)等 時(shí)間: 2025-3-28 04:31
Defining Institutional Development (ID) (BRET)-based techniques can reveal ubiquitination of GPCRs in intact cells and in real time. This chapter describes a step-by-step protocol to evaluate ubiquitination of GPCRs using the BRET methodology.作者: 時(shí)代 時(shí)間: 2025-3-28 06:50 作者: occult 時(shí)間: 2025-3-28 10:51 作者: adhesive 時(shí)間: 2025-3-28 15:10 作者: 石墨 時(shí)間: 2025-3-28 21:08 作者: 清楚說(shuō)話 時(shí)間: 2025-3-28 23:03
Book 2019ng cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in ?MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analys作者: HARP 時(shí)間: 2025-3-29 04:15
https://doi.org/10.1057/9780230620131ranslocation of RalGDS is β-arrestin-dependent and can be inhibited by either the expression of the β-arrestin1 amino-terminal domain or the expression of RalGDS clone 284 (amino acid residues 616–768 of RalGDS). We describe here a methodology for assessing GPCR-dependent stimulation of RalGDS plasma membrane translocation.作者: nonchalance 時(shí)間: 2025-3-29 11:04
https://doi.org/10.1057/9780230620131amework. We explain how it can be applied to the understanding of β-arrestin-dependent signaling networks. We illustrate our methodology through the modeling of β-arrestin recruitment kinetics at the follicle-stimulating hormone (FSH) receptor supported by in-house bioluminescence resonance energy transfer (BRET) data.作者: 窒息 時(shí)間: 2025-3-29 11:45 作者: 猛擊 時(shí)間: 2025-3-29 19:07
Workflow Description to Dynamically Model β-Arrestin Signaling Networksamework. We explain how it can be applied to the understanding of β-arrestin-dependent signaling networks. We illustrate our methodology through the modeling of β-arrestin recruitment kinetics at the follicle-stimulating hormone (FSH) receptor supported by in-house bioluminescence resonance energy transfer (BRET) data.作者: 令人苦惱 時(shí)間: 2025-3-29 23:36 作者: 洞穴 時(shí)間: 2025-3-30 01:41 作者: Limousine 時(shí)間: 2025-3-30 04:03 作者: Vulnerable 時(shí)間: 2025-3-30 12:07 作者: capsaicin 時(shí)間: 2025-3-30 15:19 作者: 使混合 時(shí)間: 2025-3-30 19:57
Using In Vitro Pull-Down and In-Cell Overexpression Assays to Study Protein Interactions with Arrest individual arrestin-3 elements in cells. Our in vitro pull-down assay with purified proteins demonstrates that relatively few elements in arrestin engage each partner, whereas cell-based functional assays indicate that certain arrestin elements devoid of other functionalities can perform individual functions in living cells.作者: 冷淡周邊 時(shí)間: 2025-3-30 23:48
