標(biāo)題: Titlebook: Basophils and Mast Cells; Methods and Protocol Bernhard F. Gibbs,Franco H. Falcone Book 2014 Springer Science+Business Media New York 2014 [打印本頁(yè)] 作者: 誤解 時(shí)間: 2025-3-21 16:32
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書目名稱Basophils and Mast Cells讀者反饋學(xué)科排名
作者: 易改變 時(shí)間: 2025-3-22 00:13
Purification of Basophils from Peripheral Human Bloodin 1879. From the first published attempts in the late 1960s, it took half a century to develop robust protocols able to provide sufficient numbers of pure, functionally unimpaired basophils. The existing protocols for basophil purification exploit those properties of basophils which distinguish the作者: 解決 時(shí)間: 2025-3-22 00:29
Mast Cell Purification Protocolsd from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason作者: START 時(shí)間: 2025-3-22 04:46
Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cellsral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phe作者: Alcove 時(shí)間: 2025-3-22 10:28 作者: 蓋他為秘密 時(shí)間: 2025-3-22 15:26 作者: 怪物 時(shí)間: 2025-3-22 20:11 作者: 眨眼 時(shí)間: 2025-3-22 21:31
Mast Cell and Basophil Cell Lines: A Compendiumnesis of allergy and inflammation, our understanding of mast cell and basophil biology is still relatively scarce. The practical difficulty in obtaining a sufficient number of purified primary cells from biological samples has slowed down the process of reaching a full understanding of the physiolog作者: yohimbine 時(shí)間: 2025-3-23 04:02
Detection of Mast Cells and Basophils by Immunohistochemistryntification of these cell types using such methods. These techniques have now been largely supplanted by immunohistochemical procedures with monoclonal antibodies directed against unique constituents of these cell types. Immunohistochemistry with antibodies specific for the granule protease tryptase作者: Tractable 時(shí)間: 2025-3-23 05:40
Measuring Histamine and Cytokine Release from Basophils and Mast Cellscribe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detecti作者: 縱火 時(shí)間: 2025-3-23 10:30
Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques Allergy 34: 332–339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor作者: 梯田 時(shí)間: 2025-3-23 15:45
Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxissential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemota作者: conquer 時(shí)間: 2025-3-23 19:07 作者: Amenable 時(shí)間: 2025-3-23 22:34 作者: 單調(diào)性 時(shí)間: 2025-3-24 04:01
Basophil Stimulation and Signaling Pathways their ability to elaborate various inflammatory mediators and cytokines remain comparatively obscure. Additionally, some studies require the analysis of pro-allergic and inflammatory mediators, such as histamine, LTC., and various basophil-derived cytokines (e.g., IL-4 and IL-13). Elucidation of in作者: Bureaucracy 時(shí)間: 2025-3-24 08:32
Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells situations, MCs are usually present at relatively low frequencies in most tissues. Since their first description, MCs in tissues were identified mostly using their morphological characteristics and their typical coloration when stained with aniline dyes. However, increasing availability of highly s作者: Meander 時(shí)間: 2025-3-24 13:01
John Lascaratos,Spyros Marketosnt studies that shed new light on the role of mast cells and basophils in health and disease. What we gain from these studies is a new appreciation for mast cells and basophils as sentinels in host defense and a further understanding that dysregulation of mast cell and basophil function can be a com作者: 頑固 時(shí)間: 2025-3-24 15:50
Ophthalmology according to Aetius Amidenus,in 1879. From the first published attempts in the late 1960s, it took half a century to develop robust protocols able to provide sufficient numbers of pure, functionally unimpaired basophils. The existing protocols for basophil purification exploit those properties of basophils which distinguish the作者: Melanoma 時(shí)間: 2025-3-24 20:13 作者: 出處 時(shí)間: 2025-3-25 01:45 作者: grotto 時(shí)間: 2025-3-25 04:08
Toichiro Kuwabara on Dave Cogan,. This method describes the isolation of mast cell progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medium. After 4 weeks, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE rec作者: conscience 時(shí)間: 2025-3-25 11:21
John Lascaratos,Spiros Marketoss mediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell i作者: 侵略 時(shí)間: 2025-3-25 15:33
https://doi.org/10.1007/978-94-011-2732-5tomated hematology analyzers and fluorescence flow cytometry. Manual basophil counting of peripheral blood smears is currently regarded as the reference method, although the limitations of this method (distribution, observer, and statistical errors) are widely recognized. Automated hematology analyz作者: 草率女 時(shí)間: 2025-3-25 16:12 作者: Eosinophils 時(shí)間: 2025-3-25 20:16 作者: 頑固 時(shí)間: 2025-3-26 02:13
A New Museum of Astronomy in Koreacribe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detecti作者: 弄污 時(shí)間: 2025-3-26 07:33
Kevin D. Pang,Kevin K. Yau,Hung-hsiang Chou Allergy 34: 332–339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor作者: Ordnance 時(shí)間: 2025-3-26 12:16 作者: CHURL 時(shí)間: 2025-3-26 16:40
Moral Philosophy in Australasia,he presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hex作者: Diatribe 時(shí)間: 2025-3-26 18:18 作者: Agronomy 時(shí)間: 2025-3-26 23:43 作者: Aids209 時(shí)間: 2025-3-27 04:18
Issues Affecting Policing 1850–1920 situations, MCs are usually present at relatively low frequencies in most tissues. Since their first description, MCs in tissues were identified mostly using their morphological characteristics and their typical coloration when stained with aniline dyes. However, increasing availability of highly s作者: 原來(lái) 時(shí)間: 2025-3-27 07:23 作者: 小說(shuō) 時(shí)間: 2025-3-27 11:20 作者: LUCY 時(shí)間: 2025-3-27 16:29
Basophils and Mast Cells978-1-4939-1173-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: musicologist 時(shí)間: 2025-3-27 18:19 作者: 滴注 時(shí)間: 2025-3-28 01:59
John Lascaratos,Spiros Marketoss mediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell interactions with fibroblasts and keratinocytes.作者: Blemish 時(shí)間: 2025-3-28 03:42 作者: 比目魚 時(shí)間: 2025-3-28 09:03
Bernhard F. Gibbs,Franco H. FalconeIncludes cutting-edge methods and protocols involving mast cells and basophils.Provides step-by-step details essential for reproducible results.Contains key notes and implementation advice from the ex作者: aggressor 時(shí)間: 2025-3-28 13:50 作者: 下垂 時(shí)間: 2025-3-28 15:47
Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cellsral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.作者: CHECK 時(shí)間: 2025-3-28 20:00 作者: 品牌 時(shí)間: 2025-3-28 23:46 作者: 軌道 時(shí)間: 2025-3-29 05:54
John Lascaratos,Spyros Marketos and basophils can function as immunoregulatory cells that modulate the immune response in health and disease. Collectively, the recent knowledge provides new challenges and opportunities towards the development of novel therapeutic strategies to augment host protection and modify disease through manipulation of mast cell and basophil function.作者: Innocence 時(shí)間: 2025-3-29 09:23
Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulatio and basophils can function as immunoregulatory cells that modulate the immune response in health and disease. Collectively, the recent knowledge provides new challenges and opportunities towards the development of novel therapeutic strategies to augment host protection and modify disease through manipulation of mast cell and basophil function.作者: 慌張 時(shí)間: 2025-3-29 15:17 作者: 殺蟲劑 時(shí)間: 2025-3-29 17:59 作者: 大暴雨 時(shí)間: 2025-3-29 23:48
Moral Philosophy in Australasia,osaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines which offer higher sensitivity and are amenable to high-throughput-scale experiments.作者: 輕浮思想 時(shí)間: 2025-3-30 01:25 作者: ICLE 時(shí)間: 2025-3-30 05:10
Ophthalmology according to Aetius Amidenus,or downstream studies. The arduous way leading up to the modern protocols is summarized in this historical retrospective. A fast protocol for purification of basophils to near homogeneity is also described.作者: 變形 時(shí)間: 2025-3-30 09:08
Purification of Basophils from Peripheral Human Bloodor downstream studies. The arduous way leading up to the modern protocols is summarized in this historical retrospective. A fast protocol for purification of basophils to near homogeneity is also described.作者: Corral 時(shí)間: 2025-3-30 12:34
Book 2014tical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also
