標題: Titlebook: Basophils and Mast Cells; Methods and Protocol Bernhard F. Gibbs,Franco H. Falcone Book 2020Latest edition Springer Science+Business Media, [打印本頁] 作者: DART 時間: 2025-3-21 17:47
書目名稱Basophils and Mast Cells影響因子(影響力)
書目名稱Basophils and Mast Cells影響因子(影響力)學科排名
書目名稱Basophils and Mast Cells網(wǎng)絡公開度
書目名稱Basophils and Mast Cells網(wǎng)絡公開度學科排名
書目名稱Basophils and Mast Cells被引頻次
書目名稱Basophils and Mast Cells被引頻次學科排名
書目名稱Basophils and Mast Cells年度引用
書目名稱Basophils and Mast Cells年度引用學科排名
書目名稱Basophils and Mast Cells讀者反饋
書目名稱Basophils and Mast Cells讀者反饋學科排名
作者: entrance 時間: 2025-3-21 23:36
The Krylov-Bogolyubov Method: ,downstream studies. The arduous way leading up to the modern protocols is summarized in this historical retrospective. A fast protocol for purification of basophils to near homogeneity is also described.作者: In-Situ 時間: 2025-3-22 02:14 作者: Certainty 時間: 2025-3-22 05:00 作者: 罐里有戒指 時間: 2025-3-22 12:07 作者: demote 時間: 2025-3-22 13:22
The Mandel’shtam-Papalexi School: , that, in this chapter, we provide detailed methods for the isolation and purification of human lung mast cells. However, it should be noted that the methods that are described in this chapter are generally applicable to the isolation of mast cells from different tissues, and this will also be discussed.作者: alabaster 時間: 2025-3-22 17:26 作者: GRILL 時間: 2025-3-23 00:19 作者: interior 時間: 2025-3-23 02:31 作者: placebo 時間: 2025-3-23 07:40 作者: 逢迎白雪 時間: 2025-3-23 10:47
Generation of Mast Cells from Murine Stem Cell Progenitorsum. After 4?weeks in culture, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE receptor expression by flow cytometry is also described. These cells are a useful tool in the determination of in vitro and in vivo mast cell function in innate and adaptive immunity.作者: Flatus 時間: 2025-3-23 15:02 作者: Muffle 時間: 2025-3-23 18:25 作者: 要素 時間: 2025-3-23 22:29
1064-3745 ation advice from the experts.This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34.+. stem ce作者: Tinea-Capitis 時間: 2025-3-24 03:17
https://doi.org/10.1007/978-3-319-17145-6 for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.作者: 小卷發(fā) 時間: 2025-3-24 06:42
Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE Fc,RI Receptor Engagement for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.作者: 分開如此和諧 時間: 2025-3-24 13:53 作者: 持續(xù) 時間: 2025-3-24 18:13
https://doi.org/10.1007/978-81-322-1961-3 and basophils can function as immunoregulatory cells that modulate the immune response in health and disease. Collectively, the recent knowledge provides new challenges and opportunities toward the development of novel therapeutic strategies to augment host protection and modify disease through manipulation of mast cell and basophil function.作者: 信條 時間: 2025-3-24 22:20
Christian Rovsing A/S from 1970 to 1984ization of the fluorescent protein in granules.??As?NPY-mRFP is preformed in granules, the reporter system?activation can be assessed using fluorescence measurements after as soon as 45–60?min, as described in this chapter, without the need to add any substrates.作者: 眉毛 時間: 2025-3-25 02:51 作者: Additive 時間: 2025-3-25 03:38
NPY-mRFP Rat Basophilic Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranization of the fluorescent protein in granules.??As?NPY-mRFP is preformed in granules, the reporter system?activation can be assessed using fluorescence measurements after as soon as 45–60?min, as described in this chapter, without the need to add any substrates.作者: 現(xiàn)實 時間: 2025-3-25 09:41
Basophils and Mast Cells978-1-0716-0696-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AIL 時間: 2025-3-25 15:22
https://doi.org/10.1007/978-3-319-55239-2ral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here, eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450?mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.作者: anaerobic 時間: 2025-3-25 18:56
The Mandel’shtam-Papalexi School: ,l functions have allowed better understanding of the function of mast cells and mast cell-mediated disorders. In this unit, we describe a protocol used for the generation and culture of peripheral CD34. stem cell-derived mast cells (PSCMCs). It provides a useful tool for the investigation of the biology of human MCs in vitro.作者: Incommensurate 時間: 2025-3-25 23:26
Computer Fiction: “A Logic Named Joe”ediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell interactions with fibroblasts and keratinocytes.作者: cataract 時間: 2025-3-26 01:29
https://doi.org/10.1007/978-1-0716-0696-4Purification; Cell culture; Signaling pathways; Nanomaterials; Knockout models作者: FRAUD 時間: 2025-3-26 08:02
978-1-0716-0698-8Springer Science+Business Media, LLC, part of Springer Nature 2020作者: chuckle 時間: 2025-3-26 09:35 作者: 陶器 時間: 2025-3-26 15:21 作者: GILD 時間: 2025-3-26 18:25 作者: Feckless 時間: 2025-3-26 23:01 作者: xanthelasma 時間: 2025-3-27 01:22
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/181439.jpg作者: Vldl379 時間: 2025-3-27 08:20
https://doi.org/10.1007/978-81-322-1961-3ent studies that shed new light on the role of mast cells and basophils in health and disease. What we gain from these studies is a new appreciation for mast cells and basophils as sentinels in host defense and a further understanding that dysregulation of mast cell and basophil function can be a co作者: grudging 時間: 2025-3-27 12:14 作者: CHURL 時間: 2025-3-27 16:01 作者: 得體 時間: 2025-3-27 18:39
https://doi.org/10.1007/978-3-319-55239-2ral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here, eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450?mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic ph作者: CRANK 時間: 2025-3-28 01:24 作者: Esophagitis 時間: 2025-3-28 03:15
https://doi.org/10.1007/978-3-319-55239-2sophil life cycle related to IgE-mediated function occurs in the blood. The same evidence suggests that IgE-mediated functionality is present for 5–7?days in the bone marrow (or other tissues) during which the cell modulates its phenotype according to local conditions. It is suggested that to proper作者: Herpetologist 時間: 2025-3-28 09:01
Reflections, Thoughts, and Episodes,mast cells. They can be sourced from wild-type, knockout, and transgenic mice and are used to repopulate mast cell-deficient mice. This method describes the isolation of mast cell hematopoietic progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medi作者: 我不重要 時間: 2025-3-28 11:50
Computer Fiction: “A Logic Named Joe”ediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell inte作者: hedonic 時間: 2025-3-28 14:55
John Impagliazzo,Timo J?rvi,Petri Pajutomated hematology analyzers and fluorescence flow cytometry. Manual basophil counting of peripheral blood smears is currently regarded as the reference method, although the limitations of this method (distribution, observer, and statistical errors) are widely recognized. Automated hematology analyz作者: 高貴領導 時間: 2025-3-28 21:39 作者: blithe 時間: 2025-3-29 00:44 作者: 被詛咒的人 時間: 2025-3-29 04:21
https://doi.org/10.1007/978-3-319-17145-6ning the allergens to be tested. These can be complex extracts, purified or recombinant allergens, or peptides. Older methods, such as the IgE CAP test, only allow sIgE measurements to multiple allergens in individual measurements. Newer technologies such as the ImmunoCAP. ISAC test allows semiquant作者: GENUS 時間: 2025-3-29 07:26 作者: 減少 時間: 2025-3-29 12:13
https://doi.org/10.1007/978-3-319-17145-6e of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosamini作者: 類人猿 時間: 2025-3-29 19:17 作者: 他日關稅重重 時間: 2025-3-29 22:01 作者: Herbivorous 時間: 2025-3-30 02:57 作者: left-ventricle 時間: 2025-3-30 04:59
Mast Cell and Basophil Cell Lines: A CompendiumFcεRI), and to test cell-stabilizing compounds. In this chapter, we review the most widely used and better-characterized MC and basophil cell lines, highlighting their advantages and drawbacks. It must be pointed out, however, that while cell lines represent a useful in vitro tool due to their easy 作者: 別炫耀 時間: 2025-3-30 11:21 作者: cumber 時間: 2025-3-30 15:37
John Impagliazzo,Timo J?rvi,Petri Paju and statistical superiority. Emerging innovative technologies for absolute cell counts include imaging flow cytometry, mass cytometry, and on-chip blood counting, but their analytical performance for absolute basophil counts is yet to be established. Here, we describe various techniques for absolut作者: 連累 時間: 2025-3-30 20:03
https://doi.org/10.1007/978-3-642-23315-9FcεRI), and to test cell-stabilizing compounds. In this chapter, we review the most widely used and better-characterized MC and basophil cell lines, highlighting their advantages and drawbacks. It must be pointed out, however, that while cell lines represent a useful in vitro tool due to their easy 作者: 招惹 時間: 2025-3-30 20:58 作者: insightful 時間: 2025-3-31 04:56
Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulatioent studies that shed new light on the role of mast cells and basophils in health and disease. What we gain from these studies is a new appreciation for mast cells and basophils as sentinels in host defense and a further understanding that dysregulation of mast cell and basophil function can be a co作者: 使絕緣 時間: 2025-3-31 08:46 作者: ovation 時間: 2025-3-31 12:53
Mast Cell Purification Protocolsd from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason作者: parasite 時間: 2025-3-31 14:54 作者: 樂器演奏者 時間: 2025-3-31 20:25
