標(biāo)題: Titlebook: Basic Techniques in Molecular Biology; Stefan Surzycki Book 2000 Springer-Verlag Berlin Heidelberg 2000 DNA.DNA Isolierung.DNA sequencing. [打印本頁] 作者: 類屬 時(shí)間: 2025-3-21 17:33
書目名稱Basic Techniques in Molecular Biology影響因子(影響力)
書目名稱Basic Techniques in Molecular Biology影響因子(影響力)學(xué)科排名
書目名稱Basic Techniques in Molecular Biology網(wǎng)絡(luò)公開度
書目名稱Basic Techniques in Molecular Biology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Basic Techniques in Molecular Biology被引頻次
書目名稱Basic Techniques in Molecular Biology被引頻次學(xué)科排名
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書目名稱Basic Techniques in Molecular Biology年度引用學(xué)科排名
書目名稱Basic Techniques in Molecular Biology讀者反饋
書目名稱Basic Techniques in Molecular Biology讀者反饋學(xué)科排名
作者: PIZZA 時(shí)間: 2025-3-21 20:26 作者: 漸變 時(shí)間: 2025-3-22 02:08
Preparation of Genomic DNA from Plant Cells, from animal or bacterial cells. Plant cells have a very tough cell wall, the breakage of which requires the application of vigorous physical force that can shear large DNA molecules. Also, plant cells accumulate large amounts of sencondarymetabolities in their vacuoles that either co-purify with nu作者: 他日關(guān)稅重重 時(shí)間: 2025-3-22 07:20
Isolation of Plasmid DNA, In most instances, plasmid DNA, isolated by these procedures, is of sufficient quality and quantity for cloning and PCR analysis. However, the plasmid DNA obtained by many of these methods is a poor template for double-stranded dideoxy DNA sequencing, largely due to inconsistency of template purity作者: Host142 時(shí)間: 2025-3-22 09:32
Isolation and Purification of RNA,, intact RNA is one of the central techniques in molecular biology and represents an important step in Northern analysis, nuclease protection assays, RNA mapping, RT-PCR, cDNA library construction and in vitro translation experiments.作者: 使服水土 時(shí)間: 2025-3-22 13:56
Isolation of PolyA+ RNA,emaining being ribosomal RNA and various species ofsmall stable RNAs. For some applications, such as construction of cDNA libraries or analysis of low-abundance mRNAs, isolation of mRNA instead oftotal cellular RNA is essential.作者: 托人看管 時(shí)間: 2025-3-22 19:13
Agarose Gel Electrophoresis of DNA,influencing the optimal separation of DNA bands in agarose gels. Protocols for running standard agarose gels and high resolution agarose gels are given, as well as a method for recovery of DNA fragments from the gel.作者: Pandemic 時(shí)間: 2025-3-22 21:38
Preparation of Probes for Hybridization,t one or both ends of nucleic acid molecules, giving specific, low density labeled probes. High density labeling is usually achieved by incorporating the reporters uniformly throughout entire length of nucleic acid molecules. For hybridization work internally labeled probes are preferred since they 作者: 組裝 時(shí)間: 2025-3-23 01:50 作者: COMMA 時(shí)間: 2025-3-23 08:33
DNA Transfer and Hybridization, technique permits hybridization of various probes to immobilized target DNA under controlled conditions, coupled with fast detection of the hybrid. Because of the sensitivity, speed, and convenience of this “solid state” hybridization procedure, it has been widely applied in applied and basic resea作者: 紅潤 時(shí)間: 2025-3-23 10:39 作者: Inelasticity 時(shí)間: 2025-3-23 16:00
DNA Cloning. General Consideration,ification of DNA fragments that can be used in subsequent experiments. Cloning involves construction of hybrid DNA molecules that are able to self-replicate in a host cell, usually bacteria. This is accomplished by inserting DNA fragments into a plasmid or bacteriophage cloning vector, introducing t作者: 加入 時(shí)間: 2025-3-23 21:27
DNA Sequencing, generates data that are not biased by previous assumption, hypotheses or experimental design. It is therefore not surprising that DNA sequencing has revealed many unexpected facts concerning gene structure, regulation of gene expression, organization of genomes, as well as, discovered new genes nev作者: urethritis 時(shí)間: 2025-3-23 23:36 作者: Abnormal 時(shí)間: 2025-3-24 05:06 作者: Acquired 時(shí)間: 2025-3-24 09:07
https://doi.org/10.1007/978-1-4615-5611-4ridized with a specific, labeled probe and results are visualized by autoradiography. The method is sensitive enough to detect mRNA present in the cell in 5 to 10 copies when analyzing 10 μg of total RNA. As little as one copy of mRNA per cell can be detected when 1 to 2 μg of poly-A. mRNA is xanalyzed(Davis et all, 1994).作者: FER 時(shí)間: 2025-3-24 12:20
Abhinav Sarje,Xiaoye S. Li,Alexander Hexemering whole genomes and their function (Hieter and Boguski, 1997). Knowing the complete sequences of a dozen microbial genomes and expected completion of sequencing of many other organisms, including humans, will without doubt change how biology is done in the future (Doolittle, 1998; DeRisi et al. 1997; Tatusow et al., 1997).作者: 爆米花 時(shí)間: 2025-3-24 17:03 作者: Intercept 時(shí)間: 2025-3-24 22:35 作者: JECT 時(shí)間: 2025-3-25 03:06 作者: 畫布 時(shí)間: 2025-3-25 04:06 作者: glucagon 時(shí)間: 2025-3-25 07:41 作者: fertilizer 時(shí)間: 2025-3-25 11:48
Challenges of Future High-End Computingrch. The Southern hybridization procedure described in this chapter is separated into two general protocols: transfer of the DNA to the membrane and hybridization of immobilized DNA to DNA or RNA probes. A description of agarose gel electrophoresis of DNA is given in Chapter 8.作者: MORT 時(shí)間: 2025-3-25 16:38
https://doi.org/10.1007/978-1-4615-5611-4he vector into bacterial cells and amplifying vector DNA using bacterial DNA replication machinery. The DNA fragment, or insert, can be derived from any organism and obtained from genomic DNA, cDNA, previously cloned DNA, PCR or synthesized in vitro.作者: 侵略主義 時(shí)間: 2025-3-25 22:25 作者: inveigh 時(shí)間: 2025-3-26 00:54 作者: Serenity 時(shí)間: 2025-3-26 04:40
PCR Analysis,st. The technique has revolutionized molecular biology and is used in virtually every area of natural sciences and medicine. The technique has cut across the boundaries separating basic and applied research, commercial technology and medicine in a way few techniques ever have.作者: seroma 時(shí)間: 2025-3-26 08:58
Book 2000hanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems..Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.作者: 才能 時(shí)間: 2025-3-26 15:42 作者: motor-unit 時(shí)間: 2025-3-26 16:54
DNA Transfer and Hybridization,rch. The Southern hybridization procedure described in this chapter is separated into two general protocols: transfer of the DNA to the membrane and hybridization of immobilized DNA to DNA or RNA probes. A description of agarose gel electrophoresis of DNA is given in Chapter 8.作者: ELUC 時(shí)間: 2025-3-26 21:35
DNA Cloning. General Consideration,he vector into bacterial cells and amplifying vector DNA using bacterial DNA replication machinery. The DNA fragment, or insert, can be derived from any organism and obtained from genomic DNA, cDNA, previously cloned DNA, PCR or synthesized in vitro.作者: EXTOL 時(shí)間: 2025-3-27 01:55 作者: Vulnerary 時(shí)間: 2025-3-27 06:36 作者: neoplasm 時(shí)間: 2025-3-27 13:04
Ahmad Afsahi,Nikitas J. Dimopoulos separated from the cytoplasm by a membrane. The nucleus contains about 90% of the total cellular DNA, the remaining DNA is in other organelles like mitochondria, chloroplasts or kinetochores. In viruses and bacteriophages, the DNA is encapsulated by a protein coat, and constitutes between 30 and 50作者: 殘廢的火焰 時(shí)間: 2025-3-27 16:11
Simulating Galaxy Formation on SMPSrids follows the hybridization reaction. Membrane hybridization is used in many different applications such as Southern and Northern blot hybridization, dot blot hybridization and phage plaque or bacterial colony hybridization. This chapter will briefly consider theoretical aspects of membrane hybri作者: GULLY 時(shí)間: 2025-3-27 19:51 作者: 沖突 時(shí)間: 2025-3-28 01:46
Ahmad Afsahi,Nikitas J. Dimopoulosand for DNA fingerprinting, restriction fragment length polymorphism (RFLP), construction of genomic or sequencing libraries and PCR analysis in research laboratories and industry. Also, DNA isolation is the first step in the study of specific DNA sequences within a complex DNA population, and in th作者: prosthesis 時(shí)間: 2025-3-28 03:23 作者: 生氣的邊緣 時(shí)間: 2025-3-28 08:58
The Future of Advanced Networks in Canada from animal or bacterial cells. Plant cells have a very tough cell wall, the breakage of which requires the application of vigorous physical force that can shear large DNA molecules. Also, plant cells accumulate large amounts of sencondarymetabolities in their vacuoles that either co-purify with nu作者: ADAGE 時(shí)間: 2025-3-28 11:19 作者: 翻動(dòng) 時(shí)間: 2025-3-28 15:05
HPTC - What Really Restrains Our Progress, intact RNA is one of the central techniques in molecular biology and represents an important step in Northern analysis, nuclease protection assays, RNA mapping, RT-PCR, cDNA library construction and in vitro translation experiments.作者: 使痛苦 時(shí)間: 2025-3-28 19:25
The Future of Advanced Networks in Canadaemaining being ribosomal RNA and various species ofsmall stable RNAs. For some applications, such as construction of cDNA libraries or analysis of low-abundance mRNAs, isolation of mRNA instead oftotal cellular RNA is essential.作者: BALE 時(shí)間: 2025-3-29 01:38 作者: hermetic 時(shí)間: 2025-3-29 04:46 作者: 迅速飛過 時(shí)間: 2025-3-29 08:43 作者: 毗鄰 時(shí)間: 2025-3-29 13:21 作者: FIS 時(shí)間: 2025-3-29 17:54
https://doi.org/10.1007/978-1-4615-5611-4orthern blot (Abwine et al, 1977). The technique is primarily used for studying gene expression, quantification of transcription, and analysis of RNA processing.It essentially consists of RNA:DNA or sense RNA and antisense RNA blot hybridization. RNA molecules are fractionated by size, using denatur作者: 啞劇 時(shí)間: 2025-3-29 21:02 作者: innate 時(shí)間: 2025-3-30 01:59
Abhinav Sarje,Xiaoye S. Li,Alexander Hexemer generates data that are not biased by previous assumption, hypotheses or experimental design. It is therefore not surprising that DNA sequencing has revealed many unexpected facts concerning gene structure, regulation of gene expression, organization of genomes, as well as, discovered new genes nev作者: myalgia 時(shí)間: 2025-3-30 06:53 作者: 初次登臺 時(shí)間: 2025-3-30 10:02
https://doi.org/10.1007/978-3-642-56968-5DNA; DNA Isolierung; DNA sequencing; Nucleic acids; Nukleins?uren; PCR; Polymerasekettenreaktion; RNA; Seuqe作者: Tailor 時(shí)間: 2025-3-30 14:28
978-3-540-66678-3Springer-Verlag Berlin Heidelberg 2000作者: Ballerina 時(shí)間: 2025-3-30 20:35 作者: Armada 時(shí)間: 2025-3-30 21:07
Isolation and Purification of RNA,, intact RNA is one of the central techniques in molecular biology and represents an important step in Northern analysis, nuclease protection assays, RNA mapping, RT-PCR, cDNA library construction and in vitro translation experiments.作者: Monotonous 時(shí)間: 2025-3-31 03:02
Isolation of PolyA+ RNA,emaining being ribosomal RNA and various species ofsmall stable RNAs. For some applications, such as construction of cDNA libraries or analysis of low-abundance mRNAs, isolation of mRNA instead oftotal cellular RNA is essential.
