標(biāo)題: Titlebook: Basic Protein and Peptide Protocols; John M. Walker Book 1994 Humana Press 1994 [打印本頁(yè)] 作者: 軍械 時(shí)間: 2025-3-21 17:16
書(shū)目名稱Basic Protein and Peptide Protocols影響因子(影響力)
書(shū)目名稱Basic Protein and Peptide Protocols影響因子(影響力)學(xué)科排名
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書(shū)目名稱Basic Protein and Peptide Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Basic Protein and Peptide Protocols被引頻次
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書(shū)目名稱Basic Protein and Peptide Protocols讀者反饋
書(shū)目名稱Basic Protein and Peptide Protocols讀者反饋學(xué)科排名
作者: 四牛在彎曲 時(shí)間: 2025-3-21 23:20 作者: Type-1-Diabetes 時(shí)間: 2025-3-22 02:34 作者: 遭遇 時(shí)間: 2025-3-22 06:29
Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins,2-D gel system has no serious rivals, with the possible exception of the Kaltschmidt and Wittmann (.) gel system for analyzing ribosomal proteins. Ribosomal proteins, however, may be adequately separated with NEPHGE.作者: cringe 時(shí)間: 2025-3-22 10:30 作者: hemophilia 時(shí)間: 2025-3-22 14:12
Relentlessly Pursuing Opportunities,trophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the “pore limit” is reached, the protein banding pattern does not change appreciably with time, although migration does not cease completely. There are three main advantages of gradient gels over linear gels:作者: Tincture 時(shí)間: 2025-3-22 18:41
The Lowry Method for Protein Quantitation, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely acceptable alternative to a rigorous absolute determination in almost all circumstances where protein mixtures or crude extracts are involved.作者: Munificent 時(shí)間: 2025-3-23 00:44
Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins,trophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the “pore limit” is reached, the protein banding pattern does not change appreciably with time, although migration does not cease completely. There are three main advantages of gradient gels over linear gels:作者: 清楚說(shuō)話 時(shí)間: 2025-3-23 04:05
Michael Matthesius,Jan-Philipp Büchler the very high electroendosmotic flow caused by charged groups on the glass walls of the gel tubes. As a consequence, pH gradients are unstable and rarely extend above pH 8.0, leading to loss of basic proteins from 2-D maps. Gradients can be extended to pH 10 by special treatment of the glass IEF tubes (.) or by using horizontal flat-bed IEF (..).作者: Emasculate 時(shí)間: 2025-3-23 06:31 作者: 鳥(niǎo)籠 時(shí)間: 2025-3-23 13:33 作者: 小丑 時(shí)間: 2025-3-23 14:13 作者: Antioxidant 時(shí)間: 2025-3-23 21:15
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dime the very high electroendosmotic flow caused by charged groups on the glass walls of the gel tubes. As a consequence, pH gradients are unstable and rarely extend above pH 8.0, leading to loss of basic proteins from 2-D maps. Gradients can be extended to pH 10 by special treatment of the glass IEF tubes (.) or by using horizontal flat-bed IEF (..).作者: BLAND 時(shí)間: 2025-3-24 01:44
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the Firstsing (IEF) gels containing 9. urea and 2% (w/v) of the detergent NP-40 with discontinuous polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE) in the second dimension. This technique is described in . and is reviewed in refs. .–..作者: Maximize 時(shí)間: 2025-3-24 05:20 作者: prosthesis 時(shí)間: 2025-3-24 07:56 作者: Urea508 時(shí)間: 2025-3-24 13:59 作者: 肌肉 時(shí)間: 2025-3-24 18:54 作者: MITE 時(shí)間: 2025-3-24 19:07 作者: 裂口 時(shí)間: 2025-3-25 00:28
Financial Aspects of HCs Business Models,taining of proteins following gel electrophoresis was a major advance providing a detection sensitivity between 20–200 times higher than methods using CBB R-250, being able to detect about 0.1 ng protein/band.作者: Thyroxine 時(shí)間: 2025-3-25 05:29
SDS Polyacrylamide Gel Electrophoresis of Proteins,d or rod of length roughly proportionate to the protein’s mol wt. Thus, proteins of either acidic or basic p. form negatively charged complexes that can be separated on the bases of differences in charges and sizes by electrophoresis through a sieve-like matrix of polyacrylamide gel.作者: 信條 時(shí)間: 2025-3-25 09:18 作者: 友好 時(shí)間: 2025-3-25 13:40 作者: DUST 時(shí)間: 2025-3-25 17:44
Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique,taining of proteins following gel electrophoresis was a major advance providing a detection sensitivity between 20–200 times higher than methods using CBB R-250, being able to detect about 0.1 ng protein/band.作者: 對(duì)手 時(shí)間: 2025-3-25 22:58 作者: 絕緣 時(shí)間: 2025-3-26 03:18 作者: 緩和 時(shí)間: 2025-3-26 07:43 作者: 貞潔 時(shí)間: 2025-3-26 08:49 作者: SHRIK 時(shí)間: 2025-3-26 16:05
Identification of Glycoproteins on Nitrocellulose Membranes and Gels,uction of disulfide bonds yields a major fragment .. 2–3 × 10.), which we term a subunit. Proteolytic digestion of subunits gives rise to large glycopeptides (.. 300–500,000), which correspond to the very highly-substituted regions of the protein core.作者: licence 時(shí)間: 2025-3-26 18:21
The Release of Oligosaccharides from Glycoproteins,ype of oligosaccharide present. .-linked chains are more difficult to release as sequential glycosidase digestions (e.g., neuraminidase and .-glycosidase) will remove some but not all types of .-linked chain. For the release of all .-linked chains for further analysis a chemical method is required, 作者: Exclude 時(shí)間: 2025-3-27 00:04 作者: carbohydrate 時(shí)間: 2025-3-27 02:02
https://doi.org/10.1007/b100335n carboxyl groups of aspartic and glutamic acids are about 3.8 and 4.2, respectively, even these amino acids will contribute little to the negative charge on a protein at this pH. Thus at pH 3, all proteins are likely to be positively charged and to travel toward the cathode in an electric field.作者: filial 時(shí)間: 2025-3-27 08:56 作者: fructose 時(shí)間: 2025-3-27 12:49
Delphine Maugards,Amine Nait-alids to be either treated for fluorography or quantified by gel slicing and scintillation counting. Thus, the higher energy [.C]- and [.S]-labeled amino acids are commonly used as protein labels since they can be directly detected by autoradiography or on the phosphorimager. The low specific activity 作者: 發(fā)微光 時(shí)間: 2025-3-27 15:13
Dafina Turkeshi Ballanca,Florentina Dushiuction of disulfide bonds yields a major fragment .. 2–3 × 10.), which we term a subunit. Proteolytic digestion of subunits gives rise to large glycopeptides (.. 300–500,000), which correspond to the very highly-substituted regions of the protein core.作者: GRATE 時(shí)間: 2025-3-27 20:42
Slavica Singer,Sun?ica Oberman Peterkaype of oligosaccharide present. .-linked chains are more difficult to release as sequential glycosidase digestions (e.g., neuraminidase and .-glycosidase) will remove some but not all types of .-linked chain. For the release of all .-linked chains for further analysis a chemical method is required, 作者: 閹割 時(shí)間: 2025-3-28 01:19
The Bradford Method for Protein Quantitation, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (..).作者: 把…比做 時(shí)間: 2025-3-28 03:32 作者: 輕浮思想 時(shí)間: 2025-3-28 08:34 作者: 合法 時(shí)間: 2025-3-28 11:40
,Cultivating “No Bossing” Leadership, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of b作者: 散布 時(shí)間: 2025-3-28 15:19 作者: 名字的誤用 時(shí)間: 2025-3-28 19:09
https://doi.org/10.1007/b100335reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Commonly, about 1.4 g SDS is bound per 1 g protein, although th作者: 平庸的人或物 時(shí)間: 2025-3-29 02:01
Relentlessly Pursuing Opportunities,creasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the “pore limit” is reached, the protein 作者: machination 時(shí)間: 2025-3-29 05:26 作者: tangle 時(shí)間: 2025-3-29 08:12 作者: 行業(yè) 時(shí)間: 2025-3-29 15:15
https://doi.org/10.1007/b100335 gradient. The method involves casting a layer of support media (usually a polyacrylamide gel but agarose can also be used) containing a mixture of carrier ampholytes (low-mol-wt synthetic polyamino-polycarboxylic acids). When an electric field is applied across such a gel, the carrier ampholytes ar作者: 吞下 時(shí)間: 2025-3-29 17:27
Delphine Maugards,Amine Nait-aliick and cheap means of quantifying proteins, or, if access to a machine is available, by using a phosphorimager. In both cases, for metabolic experiments, proteins must be labeled with a radioactive isotope in vivo prior to isolation and subsequent electrophoretic analysis. The isotope chosen, of co作者: micronized 時(shí)間: 2025-3-29 21:47 作者: corn732 時(shí)間: 2025-3-30 01:47 作者: 紋章 時(shí)間: 2025-3-30 06:57 作者: A簡(jiǎn)潔的 時(shí)間: 2025-3-30 10:19
Jan-Philipp Büchler,Christian Waltertein in a sample, such as total amino acid analysis, the Biuret reaction, and the Lowry method (. ref. .), but these do not allow quantification of one protein in a mixture of several. This may be done by chromatography and estimation of the content of the appropriate peak in the elution profile by 作者: 費(fèi)解 時(shí)間: 2025-3-30 14:07 作者: BLANC 時(shí)間: 2025-3-30 19:20 作者: 色情 時(shí)間: 2025-3-30 22:03
Slavica Singer,Sun?ica Oberman Peterkao elucidate the structure of the oligosaccharide moeities present. For further studies of the protein to be carried out it is essential that the amino acid peptide bonds remain intact during the release process, whereas this is not essential for further studies on the released oligosaccharides. Here作者: 眨眼 時(shí)間: 2025-3-31 01:22
Hidden Champions and Export Success effect of glycosylation on the function of the glycoprotein. However, to fully characterize the diversity of oligosaccharide structures within a glycoprotein it is necessary to assign each oligosaccharide structure to a particular glycosylation site (i.e., the site-specific glycosylation pattern). 作者: 幾何學(xué)家 時(shí)間: 2025-3-31 08:27
Austrian and Swiss Hidden Championsult of the experiment. Second, drying a gel that is fragile may make it easier to handle (say, during optical scanning or display on an overhead projector). Third, and most important, it may be necessary to dry a gel to allow the most efficient detection of radioactive samples on it by autoradiograp作者: 小溪 時(shí)間: 2025-3-31 11:34
Basic Protein and Peptide Protocols978-1-59259-519-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: mortuary 時(shí)間: 2025-3-31 16:52 作者: Picks-Disease 時(shí)間: 2025-3-31 21:21
,Cultivating “No Bossing” Leadership, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (..).作者: forthy 時(shí)間: 2025-4-1 01:35 作者: 滲入 時(shí)間: 2025-4-1 04:51
The Lowry Method for Protein Quantitation,itive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (.) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely ac作者: foodstuff 時(shí)間: 2025-4-1 06:00
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation,to Cu. under alkaline conditions (..). The Cu. is then detected by reaction with BCA. The two assays are of similar sensitivity, but since BCA is stable under alkali conditions, this assay has the advantage that it can be carried out as a one-step process compared to the two steps needed in the Lowr作者: 彎曲的人 時(shí)間: 2025-4-1 13:03
The Bradford Method for Protein Quantitation, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of b作者: Provenance 時(shí)間: 2025-4-1 14:20 作者: 向下 時(shí)間: 2025-4-1 21:15
