標(biāo)題: Titlebook: Basic Exercises in Immunochemistry; A Laboratory Manual Alois Nowotny Book 1979Latest edition Springer-Verlag Berlin Heidelberg 1979 Assay. [打印本頁(yè)] 作者: clannish 時(shí)間: 2025-3-21 16:37
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry影響因子(影響力)
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry被引頻次
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry被引頻次學(xué)科排名
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry年度引用
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry年度引用學(xué)科排名
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry讀者反饋
書(shū)目名稱(chēng)Basic Exercises in Immunochemistry讀者反饋學(xué)科排名
作者: 收到 時(shí)間: 2025-3-22 00:11
,Henry James’s Permanent Adolescence,trophenyl sulfonate instead of dinitrofluorobenzene, more selective dinitrophenylation could be achieved. This reagent is water soluble; its removal requires simple dialysis (or gel filtration). The number of DNP groups introduced on a protein can be measured photometrically.作者: Toxoid-Vaccines 時(shí)間: 2025-3-22 00:38
Henry James and Queer Filiationwards the water phase, in the same way as lipids are oriented in cell membranes. According to Bangham (1968), the liquid crystal or liposome is a preferred phase structure of many biologic lipids in the presence of water or salt solutions.作者: concise 時(shí)間: 2025-3-22 05:46
Separation and Isolation of H and L Chains of IgGet free by the reductive cleavage, alkylation with iodoacetamide has to be carried out. The H and L chains can be separated by gel electrophoresis in 8 M urea-formic acid buffer, or by column chromatography on Sephadex G 100 equilibrated with 8 M urea in pH 6 Triscitrate buffer.作者: 象形文字 時(shí)間: 2025-3-22 10:10
Preparation of Soluble Dinitrophenyl Proteinstrophenyl sulfonate instead of dinitrofluorobenzene, more selective dinitrophenylation could be achieved. This reagent is water soluble; its removal requires simple dialysis (or gel filtration). The number of DNP groups introduced on a protein can be measured photometrically.作者: LARK 時(shí)間: 2025-3-22 13:57
Preparation and Use of Synthetic Liposomeswards the water phase, in the same way as lipids are oriented in cell membranes. According to Bangham (1968), the liquid crystal or liposome is a preferred phase structure of many biologic lipids in the presence of water or salt solutions.作者: 壁畫(huà) 時(shí)間: 2025-3-22 18:08 作者: TEM 時(shí)間: 2025-3-23 00:19
Preparation of Dinitrophenyl- and Trinitrophenyl-Labeled Erythrocytesill proceed smoothly at room temperature or at slightly elevated temperatures. The treatment of a carrier molecule or cell with these reagents will not introduce considerable unwanted changes in their structure.作者: 排名真古怪 時(shí)間: 2025-3-23 04:16
https://doi.org/10.1007/978-1-349-23891-0such procedures, but discussion of these would be beyond the scope of this Manual. Those who wish to understand the background of this phenomenon should read the relevant chapter in the book by H.R. Maurer (1971) on disc electrophoresis. . of this Exercise describes the separation of serum protein components by disc electrophoresis.作者: 埋伏 時(shí)間: 2025-3-23 06:44
James and the Limitations of Self-Therapy, the procedure used by Dr. Ah-kau Ng (NIH, National Cancer Institute, Bethesda, MD) to iodinate exposed proteins on human tumor cell surfaces, based on the publication of Phillips and Morrison (1971).作者: 松緊帶 時(shí)間: 2025-3-23 11:45
From , to ,: Jamesian Metamorphosis,atment of the enzyme does not interfere with its enzymatic activity and a high percentage of the oxidized enzyme can be bound to antibodies without interfering with the reactivity of the antibody. This procedure is given here, based on the description of Nakane and Kawaoi (1974).作者: 鄙視讀作 時(shí)間: 2025-3-23 16:13 作者: 冷淡周邊 時(shí)間: 2025-3-23 19:24
Enzymatic Labeling of Proteins with 125I the procedure used by Dr. Ah-kau Ng (NIH, National Cancer Institute, Bethesda, MD) to iodinate exposed proteins on human tumor cell surfaces, based on the publication of Phillips and Morrison (1971).作者: 占卜者 時(shí)間: 2025-3-23 23:12
Preparation of Peroxidase-Labeled Antibodyatment of the enzyme does not interfere with its enzymatic activity and a high percentage of the oxidized enzyme can be bound to antibodies without interfering with the reactivity of the antibody. This procedure is given here, based on the description of Nakane and Kawaoi (1974).作者: BLANC 時(shí)間: 2025-3-24 04:04 作者: malign 時(shí)間: 2025-3-24 09:20
Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Columnfree in the liquid that fills the spaces between the swollen gel granules (void volume). For a detailed description of the gel filtration processes the students should consult specified literature (Flodin, 1962).作者: Override 時(shí)間: 2025-3-24 12:02 作者: pineal-gland 時(shí)間: 2025-3-24 18:13 作者: Coordinate 時(shí)間: 2025-3-24 20:01 作者: RLS898 時(shí)間: 2025-3-25 02:08 作者: 食品室 時(shí)間: 2025-3-25 03:38
https://doi.org/10.1007/978-1-349-17466-9s said to be different from the native globulin. This recombination may be prevented by the addition of SH-blocking agents such as iodoacetate to the phosphate buffer in which the dialysis of the 2-mercaptoethanol and macroglobulin mixture is carried out.作者: ANTE 時(shí)間: 2025-3-25 09:00 作者: pellagra 時(shí)間: 2025-3-25 13:15 作者: 不近人情 時(shí)間: 2025-3-25 16:44 作者: extinct 時(shí)間: 2025-3-25 20:17
https://doi.org/10.1007/978-1-349-17466-9ining biologic activity. Such an attempt was made by Porter (1959) who, in his classical work, described the cleavage of rabbit antisera by papain and the Chromatographic fractionation of the fragments obtained. In the present exercise the work of Porter will be reproduced.作者: terazosin 時(shí)間: 2025-3-26 00:49
https://doi.org/10.1007/978-1-349-23891-0rophoresis. If a mixture of different components, such as serum proteins, or amino acids, is placed in this electric field, the components will migrate toward the oppositely charged electrodes. The direction and velocity of their migration is determined mainly by their net electric charge.作者: 神圣在玷污 時(shí)間: 2025-3-26 06:26
,Henry James’s Critical Fortunes,tions. The rather high specificity of the antigen-antibody reaction permits discovering antigens in situ without isolating them from the surrounding components. For these purposes, the antibody produced in vivo against those sought for antigenic macromolecules has to be labeled with a tracer.作者: Flustered 時(shí)間: 2025-3-26 09:35 作者: Torrid 時(shí)間: 2025-3-26 14:28
https://doi.org/10.1007/978-3-642-67356-6Assay; Basic; Diffusion; Immunchemie; Immunology; Isolation; Titration; hydrolysis作者: TEM 時(shí)間: 2025-3-26 20:34
978-3-540-09453-1Springer-Verlag Berlin Heidelberg 1979作者: FOR 時(shí)間: 2025-3-26 21:58 作者: 我不明白 時(shí)間: 2025-3-27 04:09 作者: Adrenal-Glands 時(shí)間: 2025-3-27 06:04 作者: 猛擊 時(shí)間: 2025-3-27 09:42
,Shakespeare and the ‘Long Arras’,The products of H-2 genes are among the most potent transplantation antigens in mice. Much attention has been devoted to the isolation and chemical characterization of these antigens, particularly because of their importance in immunogenetic studies.作者: Ascendancy 時(shí)間: 2025-3-27 16:54
Chromatographic Separation of the IgG FragmentsCarboxymethyl cellulose has been shown to be one of the most useful ion exchangers in protein chromatography, The procedure elaborated by Peterson and Sober (1956) as well as by Sober et al. (1956) gives good resolution of different proteins. This method has been applied by Porter (1959) for the separation of breakdown products of IgG globulin,作者: CAMEO 時(shí)間: 2025-3-27 21:29 作者: 嬰兒 時(shí)間: 2025-3-27 23:54
Isolation of Cell Membranes From Ascites TumorsVarious methods are described in the literature for the isolation of membranes of nucleated cells. The procedure described in this exercise is based on the method of Boone et al. (1973) with minor modifications as applied to the isolation of TA3-Ha murine ascites tumor membrane in the author’s laboratory (Nowotny et al., 1976).作者: 晚來(lái)的提名 時(shí)間: 2025-3-28 05:51
Isolation of Murine Transplantation Antigen PreparationThe products of H-2 genes are among the most potent transplantation antigens in mice. Much attention has been devoted to the isolation and chemical characterization of these antigens, particularly because of their importance in immunogenetic studies.作者: Foregery 時(shí)間: 2025-3-28 06:16
Honour, debt, the rejection and St Paul procedure for gamma globulin isolation, this is still the simplest and one of the most efficient methods if one does not wish to separate the different Immunoglobulin fractions from each other. This method gives a preparation which contains almost all of the Immunoglobulins together with other noni作者: LAVA 時(shí)間: 2025-3-28 12:17 作者: Comedienne 時(shí)間: 2025-3-28 16:15 作者: LANCE 時(shí)間: 2025-3-28 20:19 作者: Tempor 時(shí)間: 2025-3-29 01:38
https://doi.org/10.1007/978-1-349-17466-9ining biologic activity. Such an attempt was made by Porter (1959) who, in his classical work, described the cleavage of rabbit antisera by papain and the Chromatographic fractionation of the fragments obtained. In the present exercise the work of Porter will be reproduced.作者: Ledger 時(shí)間: 2025-3-29 05:43
The Altar of Henry James (1943),ld reducing agents such as dithio-threitol, no further degradation and denaturation of the split products will occur. To block the sulfhydryl groups set free by the reductive cleavage, alkylation with iodoacetamide has to be carried out. The H and L chains can be separated by gel electrophoresis in 作者: uncertain 時(shí)間: 2025-3-29 09:02
,The Fearful Self Henry James’s , (1965),gand may be an antigen, or an enzyme substrate or enzyme inhibitor, or other molecule which can react specifically with certain molecules. The column filled with such insoluble matrix containing a covalently bound antigen can be used to separate the specific antibodies by passing serum through this 作者: 單挑 時(shí)間: 2025-3-29 14:38
https://doi.org/10.1007/978-1-349-23891-0rophoresis. If a mixture of different components, such as serum proteins, or amino acids, is placed in this electric field, the components will migrate toward the oppositely charged electrodes. The direction and velocity of their migration is determined mainly by their net electric charge.作者: 內(nèi)部 時(shí)間: 2025-3-29 18:10
https://doi.org/10.1007/978-1-349-23891-0 resolving electrophoresis procedures in gel. While regular paper or cellulose acetate paper electrophoresis detected a maximum of 6 to 7 components in a human serum sample, electrophoresis in Polyacrylamide gel separates 15 or more components with ease. Particularly sharp electrophoretic separation作者: Pigeon 時(shí)間: 2025-3-29 23:43 作者: 哀悼 時(shí)間: 2025-3-30 02:16
James and the Limitations of Self-Therapy,e changes in the structure of the labeled preparations. To circumvent this, iodination by peroxidase enzyme-containing or peroxide generating systems was applied by numerous authors since the first short report on the feasibility of this by Keston (1944), It has been used for Immunoglobulin labeling作者: outrage 時(shí)間: 2025-3-30 08:05 作者: Arthr- 時(shí)間: 2025-3-30 10:16 作者: Extricate 時(shí)間: 2025-3-30 13:18 作者: PURG 時(shí)間: 2025-3-30 16:38 作者: 種子 時(shí)間: 2025-3-30 22:57
Isolation of IgG by DEAE Cellulose co-workers (1956). This procedure is especially useful for isolation of IgG antibodies, which leave the column in a relatively pure form right after the void volume. This method is slightly modified as described here in ., using a continuous gradient elution.作者: 愛(ài)管閑事 時(shí)間: 2025-3-31 02:45 作者: 使?jié)M足 時(shí)間: 2025-3-31 05:54
Cellulose Acetate Paper Electrophoresis of Human Serumrophoresis. If a mixture of different components, such as serum proteins, or amino acids, is placed in this electric field, the components will migrate toward the oppositely charged electrodes. The direction and velocity of their migration is determined mainly by their net electric charge.作者: reaching 時(shí)間: 2025-3-31 13:15
Conjugation of Antibodies with Fluorescein and Rhodaminetions. The rather high specificity of the antigen-antibody reaction permits discovering antigens in situ without isolating them from the surrounding components. For these purposes, the antibody produced in vivo against those sought for antigenic macromolecules has to be labeled with a tracer.作者: Corral 時(shí)間: 2025-3-31 17:22 作者: LANCE 時(shí)間: 2025-3-31 19:13 作者: 骨 時(shí)間: 2025-3-31 23:45
Isolation of IgG by DEAE Cellulose co-workers (1956). This procedure is especially useful for isolation of IgG antibodies, which leave the column in a relatively pure form right after the void volume. This method is slightly modified as described here in ., using a continuous gradient elution.作者: Mindfulness 時(shí)間: 2025-4-1 03:58 作者: 土坯 時(shí)間: 2025-4-1 06:52 作者: 敲竹杠 時(shí)間: 2025-4-1 10:27 作者: 圓錐體 時(shí)間: 2025-4-1 16:58
Affinity Chromatography and Its Application to Antibody Isolationgand may be an antigen, or an enzyme substrate or enzyme inhibitor, or other molecule which can react specifically with certain molecules. The column filled with such insoluble matrix containing a covalently bound antigen can be used to separate the specific antibodies by passing serum through this 作者: fatuity 時(shí)間: 2025-4-1 20:57
Cellulose Acetate Paper Electrophoresis of Human Serumrophoresis. If a mixture of different components, such as serum proteins, or amino acids, is placed in this electric field, the components will migrate toward the oppositely charged electrodes. The direction and velocity of their migration is determined mainly by their net electric charge.作者: 祖?zhèn)髫?cái)產(chǎn) 時(shí)間: 2025-4-1 23:33
