標(biāo)題: Titlebook: Basic DNA and RNA Protocols; Adrian J. Harwood Book 1996 Humana Press 1996 [打印本頁(yè)] 作者: GUST 時(shí)間: 2025-3-21 20:07
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書(shū)目名稱(chēng)Basic DNA and RNA Protocols讀者反饋學(xué)科排名
作者: 拖債 時(shí)間: 2025-3-21 22:02 作者: coddle 時(shí)間: 2025-3-22 03:54 作者: 愚蠢人 時(shí)間: 2025-3-22 05:35 作者: 廣大 時(shí)間: 2025-3-22 11:24 作者: 縱火 時(shí)間: 2025-3-22 16:54
Hybridization in Flow Cytometry) have been described in the preceding chapters (.. and .). This chapter describes the detection of specific DNA sequences by hybridization to a labeled probe of complementary sequence. This method is suitable for the detection of a wide range of DNA concentrations down to single-copy genes within m作者: 富足女人 時(shí)間: 2025-3-22 18:45
Methods in Leukocyte Cytochemistryhese methods offer an attractive alternative to “radioactively tagged” probes in terms of safety, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nit作者: Minatory 時(shí)間: 2025-3-22 23:44
Quantitative Fluorescence Cytometryn. Such molecules are generally coupled to a nucleoside triphosphate, such as dUTP, via a linker group. The modified nucleotide is then incorporated into the probe by an enzyme-mediated reaction, such as random prime labeling for long probes or terminal transferase-catalyzed 3′-end labeling for olig作者: Suggestions 時(shí)間: 2025-3-23 03:17 作者: Feckless 時(shí)間: 2025-3-23 08:54
Methods in Leukocyte Cytochemistry to the researcher. The labels generally fall into one of two categories: primary labels, in which a detectable signal, such as a radioisotope or a fluorophore, is introduced directly into the probe, and enzymatic labels, in which an enzyme is used to catalyze a signal-generating reaction. In the la作者: Lament 時(shí)間: 2025-3-23 12:03 作者: exorbitant 時(shí)間: 2025-3-23 15:54 作者: CBC471 時(shí)間: 2025-3-23 20:08
Myeloproliferative Neoplasms (MPNs)this range, fragments are both difficult to separate and hard to visualize because of diffusion within the gel matrix. These problems are solved by native polyacrylamide gel electrophoresis (PAGE). Using native PAGE, fragments as small as 10 bp and up to 1 kb can be separated with a resolution of as作者: ENDOW 時(shí)間: 2025-3-23 23:43 作者: 表示向下 時(shí)間: 2025-3-24 04:13
Techniques in Molecular Hematology, to visualize by ethidium bromide staining. This can be a serious problem when separating small fragments by polyacrylamide gel electrophoresis (..). End-labeling has the second advantage that it can be used to produce fragments labeled at one end. Because all of the enzymes employed are specific to作者: FLASK 時(shí)間: 2025-3-24 08:50 作者: DEBT 時(shí)間: 2025-3-24 12:41
https://doi.org/10.1007/978-3-642-82734-1in RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.作者: Emmenagogue 時(shí)間: 2025-3-24 16:57 作者: 凹處 時(shí)間: 2025-3-24 19:10
Basic DNA and RNA Protocols978-1-59259-251-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 托運(yùn) 時(shí)間: 2025-3-25 02:42 作者: arthroplasty 時(shí)間: 2025-3-25 06:14
Kapil Bhalla,Celalettin Ustun,Warren Fiskusf nucleic acid at the expense of the other. Frequently, when cellular material is limiting, it is desirable to isolate both RNA and DNA from the same source. Such is the case for biopsy specimens, primary cell lines, or manipulated embryonic stem cells.作者: ascend 時(shí)間: 2025-3-25 07:54 作者: 刺耳 時(shí)間: 2025-3-25 13:24 作者: 里程碑 時(shí)間: 2025-3-25 18:09
https://doi.org/10.1007/978-3-642-82734-1in RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.作者: NAVEN 時(shí)間: 2025-3-25 22:50 作者: 兩棲動(dòng)物 時(shí)間: 2025-3-26 03:27 作者: Diastole 時(shí)間: 2025-3-26 06:27
Native Polyacrylamide Gel Electrophoresisthis range, fragments are both difficult to separate and hard to visualize because of diffusion within the gel matrix. These problems are solved by native polyacrylamide gel electrophoresis (PAGE). Using native PAGE, fragments as small as 10 bp and up to 1 kb can be separated with a resolution of as little as 1 in 500 bp.作者: Oligarchy 時(shí)間: 2025-3-26 11:48 作者: 去世 時(shí)間: 2025-3-26 13:31
RNA Slot Blottingin RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.作者: 言外之意 時(shí)間: 2025-3-26 19:25
3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detectioner sensitivity applications, and the combination of fluorescein-labeling and enhanced chemiluminescent detection allows as little as 20 × 10. mol of homologous target to be detected within a 1-h exposure.作者: Nefarious 時(shí)間: 2025-3-26 23:14
Northern Blot Analysislar in vivo system being examined. This chapter details the method for analyzing RNA by Northern blotting, which basically involves the isolation of RNA, its size fractionation by electrophoresis, transfer to membrane, and detection by nucleic acid hybridization and autoradiography.作者: 極少 時(shí)間: 2025-3-27 02:43 作者: 貝雷帽 時(shí)間: 2025-3-27 06:34
Techniques in Molecular Hematology, the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.作者: Dawdle 時(shí)間: 2025-3-27 12:35
H. J. Deeg,D. T. Bowen,C. Niemeyer-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).作者: 水獺 時(shí)間: 2025-3-27 17:39 作者: Conduit 時(shí)間: 2025-3-27 18:33
The Preparation of Riboprobes the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.作者: 有效 時(shí)間: 2025-3-28 01:48
The RNase Protection Assay-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).作者: 人充滿活力 時(shí)間: 2025-3-28 02:30
https://doi.org/10.1385/1592590748er sensitivity applications, and the combination of fluorescein-labeling and enhanced chemiluminescent detection allows as little as 20 × 10. mol of homologous target to be detected within a 1-h exposure.作者: facetious 時(shí)間: 2025-3-28 09:40 作者: 組裝 時(shí)間: 2025-3-28 14:01
Hematologic Cytology of Storage Diseasesatured by sequential soaking of the gel in solutions containing HCl and NaOH, respectively. The denatured DNA fragments are then transferred to a solid matrix or filter (usually a nylon membrane) for subsequent hybridization to a specific labeled probe (.).作者: Ganglion 時(shí)間: 2025-3-28 17:00 作者: 老巫婆 時(shí)間: 2025-3-28 20:46 作者: Shuttle 時(shí)間: 2025-3-28 23:10 作者: Misnomer 時(shí)間: 2025-3-29 06:25
Hybridization and Competition Hybridization of Southern Blotsed probe of complementary sequence. This method is suitable for the detection of a wide range of DNA concentrations down to single-copy genes within mammalian genomic DNA (little more than 1 pg of hybridizing DNA in a total of 10 μg).作者: interrogate 時(shí)間: 2025-3-29 09:26 作者: BRAND 時(shí)間: 2025-3-29 13:28
https://doi.org/10.1007/978-94-007-5028-9o different enzymes may have the same recognition sequence, but cleave the DNA at different points within that sequence. The cleavage sites fall into three different categories, either flush (or blunt) in which the recognition site is cut in the middle, or with either 5′- or 3′-over-hangs, in which 作者: Judicious 時(shí)間: 2025-3-29 17:00 作者: KIN 時(shí)間: 2025-3-29 21:09
Methods in Leukocyte Cytochemistrye phosphatase, resulting in the production of light, which will expose a standard X-ray film. Probes produced by methods analogous to those used for NBT detection are also usable in this process. The membranes with the bound alkaline phosphatase are soaked in a dilute solution of AMPPD and then expo作者: vascular 時(shí)間: 2025-3-30 03:03 作者: 六個(gè)才偏離 時(shí)間: 2025-3-30 06:39
Methods in Leukocyte Cytochemistrych as fluorescein, biotin, and digoxygenin. An example of such a system using fluorescein-labeled probes detected by an antifluorescein alkaline phosphatase conjugate, which in turn catalyzes highly sensitive chemiluminescent detection, is described in ..作者: 造反,叛亂 時(shí)間: 2025-3-30 12:03
Techniques in Molecular Hematology, molecules can help to order restriction enzyme fragments and are a prerequisite for Maxam-Gilbert DNA sequencing (..). End-labeled synthetic oligonucleotides have numerous applications, such as short DNA sequence-specific probes for mutation screening (.); probes for gel retardation and Southwester作者: 受人支配 時(shí)間: 2025-3-30 14:11
Restriction Endonuclease Digestion of DNAo different enzymes may have the same recognition sequence, but cleave the DNA at different points within that sequence. The cleavage sites fall into three different categories, either flush (or blunt) in which the recognition site is cut in the middle, or with either 5′- or 3′-over-hangs, in which 作者: 證明無(wú)罪 時(shí)間: 2025-3-30 18:19
Agarose Gel Electrophoresisbuffer solution. However, today, the “submarine” gel system is almost universally used. In this method, the agarose gel is formed on a supporting plate, and then the plate is submerged into a tank containing a suitable electrophoresis buffer. Wells are preformed in the agarose gel with the aid of a 作者: Gentry 時(shí)間: 2025-3-31 00:30
Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizationse phosphatase, resulting in the production of light, which will expose a standard X-ray film. Probes produced by methods analogous to those used for NBT detection are also usable in this process. The membranes with the bound alkaline phosphatase are soaked in a dilute solution of AMPPD and then expo作者: 本能 時(shí)間: 2025-3-31 02:51 作者: fender 時(shí)間: 2025-3-31 08:45
The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhach as fluorescein, biotin, and digoxygenin. An example of such a system using fluorescein-labeled probes detected by an antifluorescein alkaline phosphatase conjugate, which in turn catalyzes highly sensitive chemiluminescent detection, is described in ..作者: 裝入膠囊 時(shí)間: 2025-3-31 09:41 作者: Canvas 時(shí)間: 2025-3-31 14:25 作者: 無(wú)聊的人 時(shí)間: 2025-3-31 18:13
Restriction Endonuclease Digestion of DNA enzymes that cleave duplex DNA at specific target sequences with the production of defined fragments. These enzymes can be purchased from the many manufacturers of biotechnology products. The nomenclature of enzymes is based on a simple system, proposed by Smith and Nathans (.). The name of the enz作者: DUST 時(shí)間: 2025-3-31 22:12 作者: heckle 時(shí)間: 2025-4-1 03:15
Capillary Blotting of Agarose Gelsheir size in an agarose gel (.). The DNA is then partially cleaved by depurination (to facilitate the transfer of larger DNA fragments) and alkali denatured by sequential soaking of the gel in solutions containing HCl and NaOH, respectively. The denatured DNA fragments are then transferred to a soli作者: Pastry 時(shí)間: 2025-4-1 09:23
Random Primed 32P-Labeling of DNA and Vogelstein (.), is a method of incorporating radioactive nucleotides along the length of a fragment of DNA. Random primed labeling can give specific activities of between 2 × 10. and 5 × 10. dpm/μg (..). The method below is essentially that described by Feinberg and Vogelstein (.) in which a DN作者: 不如樂(lè)死去 時(shí)間: 2025-4-1 13:47
Hybridization and Competition Hybridization of Southern Blots) have been described in the preceding chapters (.. and .). This chapter describes the detection of specific DNA sequences by hybridization to a labeled probe of complementary sequence. This method is suitable for the detection of a wide range of DNA concentrations down to single-copy genes within m作者: Cantankerous 時(shí)間: 2025-4-1 14:51
Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizationshese methods offer an attractive alternative to “radioactively tagged” probes in terms of safety, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nit
