作者: 揭穿真相 時間: 2025-3-21 21:40
http://image.papertrans.cn/b/image/180996.jpg作者: 平靜生活 時間: 2025-3-22 01:11 作者: Vaginismus 時間: 2025-3-22 04:48 作者: Loathe 時間: 2025-3-22 12:38 作者: LAVE 時間: 2025-3-22 14:51 作者: Dealing 時間: 2025-3-22 19:46
Faalangst/examenvrees van (t)huis uit,croscope. The most important modifications are (1) a strong illumination system that provides suitable wavelengths for exciting a specific fluorochrome, (2) some mechanism to limit the illumination beam to specific wavelengths so that only the fluorochrome of choice is excited, and (3) a means to im作者: Abrupt 時間: 2025-3-23 01:10 作者: chassis 時間: 2025-3-23 02:11
Onderhandelen in partnerrelatietherapie,ent when visualized in brightfield or various types of interference-based light microscopy (LM). However, for the unambiguous identification and localization of most biological molecular or macromolecular elements within a structural framework, some type of staining/labeling must be employed. This i作者: APNEA 時間: 2025-3-23 07:43 作者: 發(fā)炎 時間: 2025-3-23 13:40 作者: 平躺 時間: 2025-3-23 15:21 作者: obscurity 時間: 2025-3-23 21:59 作者: HEDGE 時間: 2025-3-23 23:09
Assessing the Couple’s Adjustment to Cancerr the purpose of reconstructing microscopic objects in three dimensions (Carlsson and Aslund Appl. Opt. 26:3232–3238, 1987). There are many ways, and reasons, to reconstruct confocal image data. As an example, consider the sample of embryonic mouse heart shown in Fig. . reconstructed using a variety作者: Customary 時間: 2025-3-24 05:55 作者: 主動脈 時間: 2025-3-24 09:05
Help for People Coping with Physical Change,standing of how the system works. Unfortunately, an additional factor affecting image collection is that often students and technologists are given a preconceived idea of what the final image or data should look like. With the variety of mechanisms available on a confocal system for changing the app作者: dry-eye 時間: 2025-3-24 13:50 作者: 帽子 時間: 2025-3-24 15:46 作者: 座右銘 時間: 2025-3-24 20:46
Expanding the Couple’s Coping Skills disk confocal systems, and super-resolution systems. New developments are continually being added to the hardware and software of these microscopes to improve their performance, but the majority of confocal systems will fall into one of these groups.作者: 寵愛 時間: 2025-3-25 00:58 作者: arthrodesis 時間: 2025-3-25 07:17
Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages, disk confocal systems, and super-resolution systems. New developments are continually being added to the hardware and software of these microscopes to improve their performance, but the majority of confocal systems will fall into one of these groups.作者: intangibility 時間: 2025-3-25 10:54 作者: Reverie 時間: 2025-3-25 14:26
Analysis of Image Similarity and Relationship, determining image similarity including our perception of color in images. In addition, the correct use of several commonly accepted methods such as Pearson’s correlation coefficient, Manders’ overlap coefficient, and Spearman’s ranked correlation coefficient is discussed.作者: nephritis 時間: 2025-3-25 17:43
Faalangst/examenvrees van (t)huis uit,ses, these basic principles are also applicable to wide-field fluorescence microscopy. The chapter also reviews factors that alter or limit the fluorescent signal generated by the sample and discusses new fluorescent probes being developed to overcome some issues related to low signal yield.作者: 旋轉(zhuǎn)一周 時間: 2025-3-25 21:32 作者: Commemorate 時間: 2025-3-26 02:32
Help for People Coping with Physical Change,eir data. This chapter provides 12 guiding principles that need to be addressed when collecting and processing digital images with confocal and other microscopes and a list of resources available for assistance with confocal imaging.作者: Pathogen 時間: 2025-3-26 08:13
The Theory of Fluorescence,ses, these basic principles are also applicable to wide-field fluorescence microscopy. The chapter also reviews factors that alter or limit the fluorescent signal generated by the sample and discusses new fluorescent probes being developed to overcome some issues related to low signal yield.作者: Small-Intestine 時間: 2025-3-26 09:57 作者: 散布 時間: 2025-3-26 13:39 作者: CLAN 時間: 2025-3-26 18:54 作者: Bouquet 時間: 2025-3-26 22:24 作者: 孵卵器 時間: 2025-3-27 02:55
Introduction and Historical Perspective,icroscopy Workshop at several venues, we have found that students and technicians who are novice users of confocal microscopes are often instructed by their mentors to go to the confocal system and collect some images. Often the implied message is that it should be easy and quick since it is only a 作者: Creatinine-Test 時間: 2025-3-27 07:06
The Theory of Fluorescence,confocal microscope is used in conjunction with fluorescent samples. Fluorescence imaging provides a specific, high-contrast signal that maximally exploits the ability of the confocal microscope to remove out-of-focus light. For this reason, it is imperative for confocal microscopists to have a basi作者: 小臼 時間: 2025-3-27 10:56 作者: Celiac-Plexus 時間: 2025-3-27 16:36
Specimen Preparation,hips along the optical (.) axis as well as in the .-. plane. In other words, the investigation of spatial relationships is being evaluated in at least three dimensions. Often, this involves the collection of a series of planar images along the .axis and the reconstruction of the data into an image t作者: Stable-Angina 時間: 2025-3-27 21:45 作者: MORPH 時間: 2025-3-27 22:02 作者: Irrepressible 時間: 2025-3-28 03:46
Confocal Digital Image Capture, this goal is to match, as closely as possible, the parameters of all hardware and software in the image collection train. For reasons outlined in this chapter, it is particularly important to match the resolution of your digital image to the output resolution from the microscope. In Chap. ., we dis作者: 壕溝 時間: 2025-3-28 10:20
Types of Confocal Instruments: Basic Principles and Advantages and Disadvantages,oken down into four basic categories: single-photon point-scanning confocal systems, multiphoton (nonlinear) point-scanning confocal systems, spinning disk confocal systems, and super-resolution systems. New developments are continually being added to the hardware and software of these microscopes t作者: HPA533 時間: 2025-3-28 10:24 作者: 激怒某人 時間: 2025-3-28 17:28 作者: Glossy 時間: 2025-3-28 19:46
Analysis of Image Similarity and Relationship,organization and colocalization in fixed and live samples. However, a number of factors could?affect these analyses, and colocalization is a misnomer. We propose that?image similarity coefficient?as a better and more descriptive term. In this chapter we will discuss many of the factors involved with作者: Panacea 時間: 2025-3-29 01:53 作者: 潛移默化 時間: 2025-3-29 06:22 作者: harangue 時間: 2025-3-29 07:34
Verbinding via de begeleiding door derden,roperly and a lack of understanding of how to operate the confocal microscope in a way that maintains the fidelity of the specimen information. In too many instances, this lack of user training is exacerbated further because there is little or no daily oversight of the setup and maintenance of the m作者: 一大塊 時間: 2025-3-29 13:50
Faalangst/examenvrees van (t)huis uit,ed than in standard wide-field fluorescence. For this reason, understanding what limits the signal projected by the specimen and how to minimize signal loss is even more critical in confocal microscopy. In this chapter we review how to optimize instrument setup to minimize signal loss.作者: 神秘 時間: 2025-3-29 19:09
https://doi.org/10.1007/978-90-313-6406-0ng the .-axis is merged into a single 2-D image. Because of the added need to preserve the 3-D structure for confocal images, methods suitable for preparing samples for widefield microscopy must often be altered to accommodate the additional demand of preserving relationships in the third dimension.作者: Calculus 時間: 2025-3-29 21:23 作者: Barter 時間: 2025-3-30 00:20
What Is the Stage Climbing Process?,mage capture hardware to optimize confocal image acquisition. Two rapidly advancing areas of confocal microscopy are development of newer methods of producing excitation beams and advances in image capture equipment. We discuss these advances and compare methodologies as they relate to confocal micr作者: 放棄 時間: 2025-3-30 04:10
Enhancing Sexuality and Body Imagenction is hidden in the multiple menus necessary to operate the system. Once the basic principles are understood, it is possible to quickly learn to operate any confocal microscope and to obtain publication quality images.
