作者: Ossification 時(shí)間: 2025-3-21 21:41 作者: 符合規(guī)定 時(shí)間: 2025-3-22 01:10 作者: LAIR 時(shí)間: 2025-3-22 08:18 作者: hermitage 時(shí)間: 2025-3-22 10:16 作者: 歡樂(lè)中國(guó) 時(shí)間: 2025-3-22 13:50
?An dem Webstuhl des Gedankens“ gel electrophoresis and then transferred from the gel to a filter so that their relative positions are preserved. The fragments of interest are identified by hybridization with a complementary nucleic acid probe.作者: Optometrist 時(shí)間: 2025-3-22 17:03
https://doi.org/10.1007/978-3-662-64170-5ion mix and used for subsequent studies. Commercially available in vitro translation extracts also allow the co-translation of several different proteins simultaneously, thus providing a system to study protein:protein interactions as well as protein:DNA and protein: RNA interactions.作者: 雀斑 時(shí)間: 2025-3-22 23:46 作者: 紀(jì)念 時(shí)間: 2025-3-23 02:52
Protein Synthesis in Cell-Free Systems,ion mix and used for subsequent studies. Commercially available in vitro translation extracts also allow the co-translation of several different proteins simultaneously, thus providing a system to study protein:protein interactions as well as protein:DNA and protein: RNA interactions.作者: Interdict 時(shí)間: 2025-3-23 08:35
cDNA Synthesis and Cloning,alysis and in situ hybridization. Another important application of RNA templates is the construction of representa-tive cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukaryotic cells (Belyaysky 1989).作者: scoliosis 時(shí)間: 2025-3-23 13:35
PCR-Based Site-Specific Mutagenesis,on of DNA for different needs to be made more rapidly and easily than was previously possible. In the course of mutagenesis the relevant sequence changes can be introduced more readily by chemically synthesized oligonucleotide primers than by manipulating DNA fragments with restriction and ligation enzymes.作者: attenuate 時(shí)間: 2025-3-23 17:15
Immunological Methods for Analysis of Recombinant Proteins,translational products of cloned genes can be used. Selected clones could be chosen for further studies such as determination of their primary structure by DNA sequencing and for characterization of an appropriate expressed protein.作者: labile 時(shí)間: 2025-3-23 21:19
Book 1998interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.作者: 工作 時(shí)間: 2025-3-24 01:44
Adornos ambivalente Heine-Rezeptionalysis and in situ hybridization. Another important application of RNA templates is the construction of representa-tive cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukaryotic cells (Belyaysky 1989).作者: 袖章 時(shí)間: 2025-3-24 03:54 作者: 婚姻生活 時(shí)間: 2025-3-24 06:52
Heine’s Flowers in Schumann’s ?Myrthen“translational products of cloned genes can be used. Selected clones could be chosen for further studies such as determination of their primary structure by DNA sequencing and for characterization of an appropriate expressed protein.作者: 碳水化合物 時(shí)間: 2025-3-24 13:05 作者: Anterior 時(shí)間: 2025-3-24 17:27
Adornos ambivalente Heine-Rezeptionng DNA. Both of these methods depend on analytical polyacrylamide gel electrophoresis to resolve oligonucleotides with one identical end and one end varying in length by a single nucleotide. The enzymatic method has been improved over recent years. The Sanger method was developed as a basic method f作者: 糾纏 時(shí)間: 2025-3-24 21:18
Zwischen Europa und ?Nazionalkatzenjammer“ant DNA technology. Nucleotide changes are necessary not only for the analysis of the structural basis of gene and corresponding protein function, but also for the generation of novel gene products. The availability of the polymerase chain reaction (PCR) in the last decade has enabled the modificati作者: cylinder 時(shí)間: 2025-3-24 23:29
Zwischen Europa und ?Nazionalkatzenjammer“ication, recombination and DNA condensation in chromatin are steered by binding of regulatory protein ligands to specific sites in DNA. Numerous methods have been developed to study protein-DNA interactions. In this chapter we discuss two widely used and straightforward approaches to address this pr作者: 犬儒主義者 時(shí)間: 2025-3-25 06:26
?An dem Webstuhl des Gedankens“derable ease and speed. In an electric field nucleic acids move through the pores in the gel towards the anode according to their molecular weight. After electrophoresis, the location of nucleic acids within the gel can be directly visualized in ultraviolet light by a dye ethidium bromide. If desire作者: RACE 時(shí)間: 2025-3-25 09:39 作者: 好開(kāi)玩笑 時(shí)間: 2025-3-25 14:30 作者: 膠水 時(shí)間: 2025-3-25 17:45 作者: 腐敗 時(shí)間: 2025-3-25 21:42 作者: 不能和解 時(shí)間: 2025-3-26 02:40
https://doi.org/10.1007/978-3-642-71965-3DNA; RNA; gene; nucleic acid; protein; protein synthesis; sequencing; synthesis作者: WATER 時(shí)間: 2025-3-26 05:23 作者: voluble 時(shí)間: 2025-3-26 11:27 作者: squander 時(shí)間: 2025-3-26 15:16 作者: 未成熟 時(shí)間: 2025-3-26 20:01 作者: diabetes 時(shí)間: 2025-3-26 21:49
RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase,The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.作者: CLAM 時(shí)間: 2025-3-27 03:01 作者: Ankylo- 時(shí)間: 2025-3-27 08:14 作者: acclimate 時(shí)間: 2025-3-27 09:58
PCR-Based Site-Specific Mutagenesis,ant DNA technology. Nucleotide changes are necessary not only for the analysis of the structural basis of gene and corresponding protein function, but also for the generation of novel gene products. The availability of the polymerase chain reaction (PCR) in the last decade has enabled the modificati作者: 白楊魚 時(shí)間: 2025-3-27 15:25 作者: Foreshadow 時(shí)間: 2025-3-27 19:20
Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids,derable ease and speed. In an electric field nucleic acids move through the pores in the gel towards the anode according to their molecular weight. After electrophoresis, the location of nucleic acids within the gel can be directly visualized in ultraviolet light by a dye ethidium bromide. If desire作者: hankering 時(shí)間: 2025-3-28 01:39
Immunological Methods for Analysis of Recombinant Proteins,inant DNA in . cells many clones are usually produced, and the same situation appears if recombinant DNA expression libraries are available. Furthermore, if appropriate monoclonal or polyclonal antibodies are available, the method of immunoscreening of colonies for direct immunological detection of 作者: constitutional 時(shí)間: 2025-3-28 03:12 作者: 事物的方面 時(shí)間: 2025-3-28 08:37
10樓作者: Graduated 時(shí)間: 2025-3-28 14:05
10樓作者: Compassionate 時(shí)間: 2025-3-28 16:57
10樓作者: 不可救藥 時(shí)間: 2025-3-28 22:18
10樓
