作者: intoxicate 時間: 2025-3-21 23:21 作者: 拋棄的貨物 時間: 2025-3-22 01:15 作者: 破裂 時間: 2025-3-22 05:34
Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts,m a single primary culture derived from a biopsy. This bank of stable diploid cells thus has many attributes usually only found in transformed cell lines where homogeneity of cell population over a prolonged period of time is often crucial to the experimenter. Unlike a transformed line however, huma作者: 喚醒 時間: 2025-3-22 11:47
Scale-Up of Suspension and Anchorage-Dependent Animal Cells,complete expression and contamination with bacterial toxins, and more importance is now being put on expression of recombinant proteins from mammalian cells. This has allowed the use of faster growing and less fastidious cell lines, such as CHO, and amplification of product expression by multiple co作者: 減去 時間: 2025-3-22 14:04
Book 19972nd editionce and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo‘s valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ................................................................................作者: Myofibrils 時間: 2025-3-22 17:15
,Sexualit?t im Alter — ein Tabu?,d the isolation of genetically pure strains. The cells described are all derived from Chinese hamsters since to date, these cells have proved to be the most useful for somatic cell genetics (.,.). The techniques, however, are generally applicable to most fibroblast cell types.作者: cataract 時間: 2025-3-22 21:41
Nachruf auf Professor Pierre Grappinrrow. This layer, termed “stromal” layer because of its likely derivation from the supportive elements in the marrow, has been extensively characterised (.). Stromal cells have been shown to exert positive and negative regulatory effects on hematopoietic cells through cell-cell interaction and by the secretion of cytokines (.,.).作者: 枕墊 時間: 2025-3-23 01:55 作者: 上漲 時間: 2025-3-23 08:01 作者: 沙漠 時間: 2025-3-23 11:31
Primary Kidney Cells,ral reasons. First of all, kidney cells can be grown in vitro from the animal of choice. Thus the results of tissue-culture studies can be more closely correlated with animal studies. Second, new tissue-culture systems can be developed that more closely resemble the kidney cells in vivo than presently available established kidney cell lines.作者: 躲債 時間: 2025-3-23 16:44
Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Ceproliferation and differentiation of early B-lymphocyte lineage cells. This method can be used to obtain heterogeneous populations of immature precursors of the B-cell lineage greatly enriched from other hematopoietic cell types. Clonal populations can also be obtained by extension of this method to limiting dilution culture.作者: Multiple 時間: 2025-3-23 19:20
1064-3745 The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the m作者: 叢林 時間: 2025-3-23 23:38 作者: Between 時間: 2025-3-24 03:37
https://doi.org/10.1007/978-3-476-05543-9ies. In humans, current theories are that B-lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T- and B-cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).作者: 胰臟 時間: 2025-3-24 09:05
https://doi.org/10.1007/978-3-476-03711-4dy of skin epithelial stem cells because the epithelial cells of the outer root sheaths of the hair follicle are pluri-potential in their phenotype, and can re-epithelialize the denuded epidermis to heal wounds (.).作者: 使聲音降低 時間: 2025-3-24 14:27
Ideenkampf und ?pacifìke Mission?eover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37°C, for up to a few days, and when obtained in suspension, are easy to freeze (with 10% dimethyl sulfoxide [DMSO] as a cryoprotectant) and store in liquid nitrogen.作者: defendant 時間: 2025-3-24 17:43
https://doi.org/10.1007/978-3-476-03651-3ryonic stem cell lines is that they can be manipulated to differentiate into a diversity of cell types either directly in the tissue-culture dish or within the context of the normal developing embryo following their return to the embryonic environment.作者: 格言 時間: 2025-3-24 21:40 作者: 頭腦冷靜 時間: 2025-3-24 23:21
Hollow-Fiber Cell Culture,nd removes CO. and other waste products. This description applies equally to hollow-fiber culture systems but with culture medium in the place of blood, and with capillaries made from ultrafiltration or microfiltration membranes.作者: 男生戴手銬 時間: 2025-3-25 04:34
Separation and Maintenance of Primary T- and B-Lymphocytes,ies. In humans, current theories are that B-lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T- and B-cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).作者: 利用 時間: 2025-3-25 09:11 作者: 百科全書 時間: 2025-3-25 12:19 作者: blackout 時間: 2025-3-25 17:37 作者: Isolate 時間: 2025-3-25 22:24
Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived from Boogy, elaborate cell culture techniques allow the investigation of regulatory processes and cellular interactions in in vitro systems, in which the experimental conditions can be defined as in no other experimental paradigm.作者: Cabg318 時間: 2025-3-26 02:55 作者: 進入 時間: 2025-3-26 04:28 作者: sed-rate 時間: 2025-3-26 09:52
Book 19972nd edition we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the many books 作者: 刺穿 時間: 2025-3-26 15:38
,Sexualit?t im Alter — ein Tabu?, It is not intended to describe the establishment of a tissue culture laboratory, nor to provide a historical or theoretical survey of cell culture. There are several books that adequately cover these areas, including the now somewhat dated, but still valuable volume by Paul (.), that of Freshney (.作者: 夸張 時間: 2025-3-26 19:36
,Demographische Entwicklung in ?sterreich,Hayflick (.). He established a reliable protocol for the maintenance of fibroblast strains that was also favorable for stimulating cell proliferation. The reproducibility of the cell cultivation system he developed allowed the realization that normal diploid cells exhibit a limited proliferative pot作者: MAZE 時間: 2025-3-26 21:02
https://doi.org/10.1007/978-3-476-01375-0d dynamically interacting sets of mechanisms: Mechanisms associated with epithelial-mesenchymal interactions, a relationship that is universal and that constitutes the organizational basis for all metazoan tissues; and mechanisms associated with stem cell biology and with stem cell-fed maturational 作者: gimmick 時間: 2025-3-27 03:00 作者: 寄生蟲 時間: 2025-3-27 06:14
https://doi.org/10.1007/978-3-476-05543-9h density, and are perfused via capillaries having semipermeable walls. Fluid (blood) circulating within the capillaries brings oxygen and nutrients and removes CO. and other waste products. This description applies equally to hollow-fiber culture systems but with culture medium in the place of bloo作者: 補充 時間: 2025-3-27 09:52
https://doi.org/10.1007/978-3-476-05543-9 Bursa Fabricus of birds. Mammals do not have an equivalent structure, and there are varying opinions as to the similarity of these cells between species. In humans, current theories are that B-lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T- and B-cells are mo作者: Feigned 時間: 2025-3-27 17:08 作者: 花爭吵 時間: 2025-3-27 21:32 作者: Abominate 時間: 2025-3-27 23:16
Ideenkampf und ?pacifìke Mission?which represent 60–65% of the cells of the liver and about 80% of the volume of the organ (since they are larger than the other cells) (.–.). Two years after Howard et. al. (.) discovered that collagenase was a very important tool in the separation of liver cells, Berry and Friend (.) introduced the作者: Peak-Bone-Mass 時間: 2025-3-28 02:52 作者: alcoholism 時間: 2025-3-28 08:57
https://doi.org/10.1007/978-3-476-03651-3 system for fundamental research into the cellular differentiation processes occuring in the normal embryo. Perhaps the most attractive feature of embryonic stem cell lines is that they can be manipulated to differentiate into a diversity of cell types either directly in the tissue-culture dish or w作者: 廚師 時間: 2025-3-28 14:12 作者: CANE 時間: 2025-3-28 17:37
Spannungen als autorspezifischer Strukturzugiation with bone marrow-derived stromal cells, as in long-term cultures, or by establishing factor-dependent (FDCP-Mix) primitive hemopoietic cell lines. The long-term culture technique and a reproducible method for establishing FDCP-Mix cell lines are described here (.,.).作者: 同音 時間: 2025-3-28 22:48
Rückblicke auf Personen und Zust?ndeolid culture system. These progenitor cells, initially called “Colony Forming Units in Culture” (CFU-C) developed into colonies after several days of culture in the presence of different types of conditioned media containing “hematopoietic growth factors.” They are found in bone marrow, blood, fetal作者: RODE 時間: 2025-3-29 01:00 作者: 嗎啡 時間: 2025-3-29 05:40 作者: 干旱 時間: 2025-3-29 08:23
,Sexualit?t im Alter – ein Tabu?,Since the seminal observations of Hayflick and Moorhead (.) that cultured human fibroblasts have a finite replicative lifespan, study of in vitro cellular senescence has provided a foundation for understanding organismal aging.作者: 嚴(yán)峻考驗 時間: 2025-3-29 15:00 作者: 仔細(xì)閱讀 時間: 2025-3-29 19:38 作者: Mediocre 時間: 2025-3-29 21:46
Aging of Cultured Human Skin Fibroblasts,Since the seminal observations of Hayflick and Moorhead (.) that cultured human fibroblasts have a finite replicative lifespan, study of in vitro cellular senescence has provided a foundation for understanding organismal aging.作者: ASSET 時間: 2025-3-30 01:25 作者: ligature 時間: 2025-3-30 05:04
Human Thyroid Epithelial Cells,The thyroid gland contains two populations of epithelial cells, of quite different embryological origin and function. Only the major component—the follicular cells—is considered here. The minor C cell population, which forms only a few percent, can be ignored for the purposes of primary culture.作者: BUST 時間: 2025-3-30 09:51 作者: Cognizance 時間: 2025-3-30 15:15 作者: Phenothiazines 時間: 2025-3-30 20:08
Ideenkampf und ?pacifìke Mission?which represent 60–65% of the cells of the liver and about 80% of the volume of the organ (since they are larger than the other cells) (.–.). Two years after Howard et. al. (.) discovered that collagenase was a very important tool in the separation of liver cells, Berry and Friend (.) introduced the technique of collagenase perfusion of the liver.作者: Indurate 時間: 2025-3-30 22:50 作者: 苦笑 時間: 2025-3-31 01:46
Rückblicke auf Personen und Zust?ndeolid culture system. These progenitor cells, initially called “Colony Forming Units in Culture” (CFU-C) developed into colonies after several days of culture in the presence of different types of conditioned media containing “hematopoietic growth factors.” They are found in bone marrow, blood, fetal liver, and spleen of most mammalian species.作者: committed 時間: 2025-3-31 05:38 作者: AVOID 時間: 2025-3-31 09:26
Ex Vivo Maintenance of Differentiated Mammalian Cells,d dynamically interacting sets of mechanisms: Mechanisms associated with epithelial-mesenchymal interactions, a relationship that is universal and that constitutes the organizational basis for all metazoan tissues; and mechanisms associated with stem cell biology and with stem cell-fed maturational lineages.作者: 高腳酒杯 時間: 2025-3-31 14:18 作者: 顛簸地移動 時間: 2025-3-31 21:35 作者: jeopardize 時間: 2025-4-1 00:16
Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM, CFU-E, and BFU-E,olid culture system. These progenitor cells, initially called “Colony Forming Units in Culture” (CFU-C) developed into colonies after several days of culture in the presence of different types of conditioned media containing “hematopoietic growth factors.” They are found in bone marrow, blood, fetal liver, and spleen of most mammalian species.作者: Intractable 時間: 2025-4-1 05:50
Basic Cell Culture, It is not intended to describe the establishment of a tissue culture laboratory, nor to provide a historical or theoretical survey of cell culture. There are several books that adequately cover these areas, including the now somewhat dated, but still valuable volume by Paul (.), that of Freshney (.作者: 尊敬 時間: 2025-4-1 08:20
Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts,Hayflick (.). He established a reliable protocol for the maintenance of fibroblast strains that was also favorable for stimulating cell proliferation. The reproducibility of the cell cultivation system he developed allowed the realization that normal diploid cells exhibit a limited proliferative pot作者: 談判 時間: 2025-4-1 11:57
Ex Vivo Maintenance of Differentiated Mammalian Cells,d dynamically interacting sets of mechanisms: Mechanisms associated with epithelial-mesenchymal interactions, a relationship that is universal and that constitutes the organizational basis for all metazoan tissues; and mechanisms associated with stem cell biology and with stem cell-fed maturational 作者: CANDY 時間: 2025-4-1 17:39
Scale-Up of Suspension and Anchorage-Dependent Animal Cells, that cells are now grown industrially in unit volumes of up to 10,000 L for vaccine, interferon, and monoclonal antibody production. The need to scale-up cell cultures has been expanded from the historical requirement for vaccine manufacture to include not only interferon and antibodies, but many i
