標題: Titlebook: Base Editors; Methods and Protocol Sangsu Bae,Beomjong Song Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice [打印本頁] 作者: concession 時間: 2025-3-21 17:56
書目名稱Base Editors影響因子(影響力)
書目名稱Base Editors影響因子(影響力)學科排名
書目名稱Base Editors網(wǎng)絡公開度
書目名稱Base Editors網(wǎng)絡公開度學科排名
書目名稱Base Editors被引頻次
書目名稱Base Editors被引頻次學科排名
書目名稱Base Editors年度引用
書目名稱Base Editors年度引用學科排名
書目名稱Base Editors讀者反饋
書目名稱Base Editors讀者反饋學科排名
作者: 嬉耍 時間: 2025-3-21 21:29 作者: 打折 時間: 2025-3-22 00:47
Delivering Base Editors In Vivo by Adeno-Associated Virus Vectorstissues. We provide step by step protocols for (i) designing and validating base editing systems, (ii) packaging base editors into recombinant AAV vector particles, (iii) delivering AAV to the central nervous system via intrathecal injection, and (iv) quantifying base editing frequencies by next-generation sequencing.作者: Estimable 時間: 2025-3-22 08:04 作者: Anthropoid 時間: 2025-3-22 08:46
Maximum Likelihood Hebbian Learningning-based computational models to predict the efficiencies and outcome frequencies of ABE and CBE at given target DNA sites, in silico. Here, we describe the step-by-step procedure for the accurate determination of specific target nucleotides for ABE or CBE editing on the online available web tool, (DeepBaseEditor, .).作者: 啜泣 時間: 2025-3-22 15:50 作者: 容易懂得 時間: 2025-3-22 18:08 作者: anthropologist 時間: 2025-3-22 22:38 作者: 使服水土 時間: 2025-3-23 02:52
Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seqving uracil in vitro. Digested genomic DNA is subjected to WGS, and then sequencing reads are aligned to the reference genome, resulting in straight alignments at on-target and off-target sites. The in vitro cleavage sites related to the straight alignments can be identified using the Digenome-seq computer tool.作者: 窒息 時間: 2025-3-23 08:08
Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors introduced the target nucleotide substitution in living cells and identified variants whose functions were not defined. Here, we describe the methods for the functional appraisal of BRCA1 variants using CRISPR-based base editors.作者: 他一致 時間: 2025-3-23 11:58
Book 2023an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, o作者: harrow 時間: 2025-3-23 14:55 作者: 吹牛大王 時間: 2025-3-23 19:31 作者: Lice692 時間: 2025-3-23 23:38 作者: Fatten 時間: 2025-3-24 02:31
https://doi.org/10.1007/978-3-030-28475-6tissues. We provide step by step protocols for (i) designing and validating base editing systems, (ii) packaging base editors into recombinant AAV vector particles, (iii) delivering AAV to the central nervous system via intrathecal injection, and (iv) quantifying base editing frequencies by next-generation sequencing.作者: 包租車船 時間: 2025-3-24 08:31
https://doi.org/10.1007/978-3-030-28475-6s (LNPs), as a non-viral delivery, are able to deliver the ABE mRNAs and gRNA to the target tissues (Newby and Liu, Mol Ther 29:3107–3124, 2021). This chapter mainly introduces the production and LNP delivery of ABE mRNA and gRNA.作者: MEEK 時間: 2025-3-24 11:27 作者: inferno 時間: 2025-3-24 18:22 作者: jumble 時間: 2025-3-24 20:57
Maximum Likelihood Hebbian Learningools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.作者: 整理 時間: 2025-3-25 01:59 作者: 縮短 時間: 2025-3-25 05:28 作者: 飲料 時間: 2025-3-25 07:41
https://doi.org/10.1007/978-3-030-28475-6 inherited retinal disease. The majority of retinal genome editing requires intravitreal and subretinal injection delivery of the therapeutic vector in order to transduce the target cells. Here, we provide an application guide of base editor as performed in the mouse retina.作者: CHAR 時間: 2025-3-25 13:10 作者: 幾何學家 時間: 2025-3-25 18:51
Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Micete genetically modified animals that harbor disease-causing pathogenic point mutations. In this chapter, I describe the basic protocol used to introduce disease-relevant pathogenic mutations into mice by Target-AID.作者: 哎呦 時間: 2025-3-25 20:43
Targeted Mutagenesis in Mice Using a Base Editorools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.作者: GEST 時間: 2025-3-26 00:39 作者: Audiometry 時間: 2025-3-26 07:06
Ex Vivo Base Editing Therapy with Chemically Derived Hepatic Progenitorss a safe and efficient strategy for ex vivo gene therapy. Here, we described how to generate hepatic progenitors from terminally differentiated hepatocytes, deliver base/prime editors into the cells, select corrected hepatic progenitors, and transplant them into mice of inborn error of metabolism.作者: Habituate 時間: 2025-3-26 09:22
Application of Base Editor-Mediated Genome Editing in Mouse Retina inherited retinal disease. The majority of retinal genome editing requires intravitreal and subretinal injection delivery of the therapeutic vector in order to transduce the target cells. Here, we provide an application guide of base editor as performed in the mouse retina.作者: 生命 時間: 2025-3-26 12:44 作者: Heart-Rate 時間: 2025-3-26 17:53 作者: Indolent 時間: 2025-3-26 23:55 作者: Expostulate 時間: 2025-3-27 04:58
Kernel and Nonlinear Correlationsysis of NGS data developed by Luca Pinello group, DeepBaseEditor for prediction of target efficiency developed by Hyongbum Henry Kim group, and BE-Hive for prediction of target outcome developed by David Liu group.作者: 領先 時間: 2025-3-27 07:39
Introduction and Perspectives of DNA Base Editorse fact that they can perform efficient and precise gene editing without generating a DNA double-strand break (DSB) or requiring a donor DNA template. Since they were first developed, significant efforts have been made to improve DNA base editors in order to overcome problems such as off-target edits作者: 鉤針織物 時間: 2025-3-27 11:14
Web-Based Computational Tools for Base Editorsrs with different substitution patterns, editing windows, and protospacer adjacent motif (PAM) sequences. For the design of target sequences, consideration of off-target sequences is required. In addition, for assessment of base editing outcomes in bulk populations, the analysis of high-throughput s作者: Pillory 時間: 2025-3-27 17:40 作者: insolence 時間: 2025-3-27 18:29 作者: fender 時間: 2025-3-28 01:08
A/C Simultaneous Conversion Using the Dual Base Editor in Human Cellseditors can only edit either adenines or cytosines. Thus, our lab has developed a dual base editor (A&C-BEmax) through the fusion of cytidine and adenosine deaminases to Cas9n to achieve both C?G to T?A and A?T to G?C mutations, which enables A/C simultaneous conversion in the same allele (up to 30%作者: 載貨清單 時間: 2025-3-28 04:40
Functional Analysis of Variants in BRCA1 Using CRISPR Base Editorsed to evaluate the function of the variants of uncertain significance (VUS) of the BRCA genes. However, these methods have limitations as they are associated with overexpression and do not apply to post-transcriptional regulation. Therefore, there are several VUS whose functions are unclear. Recentl作者: CAGE 時間: 2025-3-28 09:32
Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Micestranded DNA breaks. Target-AID (activation-induced cytidine deaminase) is a representative base editing tool and may serve as a potent option to create genetically modified animals that harbor disease-causing pathogenic point mutations. In this chapter, I describe the basic protocol used to introdu作者: BADGE 時間: 2025-3-28 14:19
Targeted Mutagenesis in Mice Using a Base Editored Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other 作者: 套索 時間: 2025-3-28 18:05 作者: Exterior 時間: 2025-3-28 21:53 作者: Indolent 時間: 2025-3-29 00:42 作者: 過去分詞 時間: 2025-3-29 07:00
Ex Vivo Base Editing Therapy with Chemically Derived Hepatic Progenitorsand treatment of hereditary intractable diseases and models. Small molecule-mediated reprogramming of hepatocytes into bi-potent hepatic progenitors is a safe and efficient strategy for ex vivo gene therapy. Here, we described how to generate hepatic progenitors from terminally differentiated hepato作者: 使顯得不重要 時間: 2025-3-29 10:04
Application of Base Editor-Mediated Genome Editing in Mouse Retinaintains a low rate of insertion-deletion (INDEL) errors. With these flexibility and safety, base editor has been widely used in many fields, including inherited retinal disease. The majority of retinal genome editing requires intravitreal and subretinal injection delivery of the therapeutic vector i作者: 北極熊 時間: 2025-3-29 11:48 作者: 晚來的提名 時間: 2025-3-29 19:26
Base Editors978-1-0716-2879-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: uncertain 時間: 2025-3-29 20:57 作者: NOMAD 時間: 2025-3-30 01:50 作者: CANT 時間: 2025-3-30 05:30 作者: 罵人有污點 時間: 2025-3-30 10:32
Base Editing of Human Hematopoietic Stem CellsBase editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to remedy blood disorders. Here we describe the ex vivo base editing of human CD34+ hematopoietic stem and progenitor cells (HSPCs) by electroporation of base editor mRNA or protein.作者: 萬神殿 時間: 2025-3-30 16:20 作者: 追逐 時間: 2025-3-30 19:34
Kernel and Nonlinear Correlationsrs with different substitution patterns, editing windows, and protospacer adjacent motif (PAM) sequences. For the design of target sequences, consideration of off-target sequences is required. In addition, for assessment of base editing outcomes in bulk populations, the analysis of high-throughput s作者: 支架 時間: 2025-3-30 22:44
Maximum Likelihood Hebbian Learningf interest. However, the introduction of point mutations using base editors can be difficult due to low editing efficiencies and/or the existence of multiple target nucleotides within the base editing window at the target site. Thus, previous works have relied heavily on experimentally evaluating th作者: 合并 時間: 2025-3-31 02:32
Multicollinearity and Partial Least Squaresnown as Cas12a) and programmable deaminase including cytosine base editors (CBEs) and adenine base editors (ABEs). To define the genome-wide specificity of dLbCpf1-BE (also known as dLbCas12a-BE), genomic DNA is first incubated with dLbCpf1-BE, which induces C-to-U conversion at on-target and off-ta作者: hypotension 時間: 2025-3-31 05:50 作者: Unsaturated-Fat 時間: 2025-3-31 12:55 作者: Adenoma 時間: 2025-3-31 13:47
Multicollinearity and Partial Least Squaresstranded DNA breaks. Target-AID (activation-induced cytidine deaminase) is a representative base editing tool and may serve as a potent option to create genetically modified animals that harbor disease-causing pathogenic point mutations. In this chapter, I describe the basic protocol used to introdu作者: FIR 時間: 2025-3-31 20:47
Maximum Likelihood Hebbian Learninged Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other
