標(biāo)題: Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2016Latest edition Springer Science+Business Media, LLC 2016 BEV [打印本頁(yè)] 作者: 召喚 時(shí)間: 2025-3-21 20:06
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols影響因子(影響力)
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols被引頻次
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols被引頻次學(xué)科排名
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols年度引用
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols年度引用學(xué)科排名
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols讀者反饋
書(shū)目名稱(chēng)Baculovirus and Insect Cell Expression Protocols讀者反饋學(xué)科排名
作者: chondromalacia 時(shí)間: 2025-3-21 20:41
Annika Wilhelmy,Martin KleinmannThis chapter lists the known cell lines from Lepidoptera, largely based on previous compilations of insect cell lines published by W. Fred Hink. More than 320 lines from 65 species are listed. The official designation is given for each cell line as well as the species, tissue source, and, when known, the susceptibilities to baculoviruses.作者: 變色龍 時(shí)間: 2025-3-22 02:34
Annika Wilhelmy,Martin KleinmannThis chapter describes procedures for initiating new cell lines from lepidopteran larval tissues and embryos. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed.作者: Monocle 時(shí)間: 2025-3-22 06:26
Available Lepidopteran Insect Cell LinesThis chapter lists the known cell lines from Lepidoptera, largely based on previous compilations of insect cell lines published by W. Fred Hink. More than 320 lines from 65 species are listed. The official designation is given for each cell line as well as the species, tissue source, and, when known, the susceptibilities to baculoviruses.作者: DOTE 時(shí)間: 2025-3-22 08:59 作者: 健忘癥 時(shí)間: 2025-3-22 14:17 作者: Intervention 時(shí)間: 2025-3-22 20:38
Heinz Kindler,Adelheid Unterstallernsect cell system. The production and scale-up of these particles, which are mostly sought as vaccine candidates, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. The use of host insect cells allows mass production of VLPs in a proven scalable system.作者: 逗留 時(shí)間: 2025-3-23 00:33
Karl Poppers Sp?twerk und seine ?Welt 3“ model system (e.g., for studying apoptosis). The fundamental techniques involved in these applications are described throughout this book. In this chapter the most widely used techniques are summarized and the reader is directed to detailed information found elsewhere in this book. Furthermore, man作者: Hangar 時(shí)間: 2025-3-23 04:42
Karl Popper und Friedrich August von Hayektion in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure and their replication in insect larvae. Baculovirus gene expression occurs in an ordered cascade, regulated by early, late作者: 強(qiáng)制令 時(shí)間: 2025-3-23 06:31 作者: Vital-Signs 時(shí)間: 2025-3-23 11:36 作者: Laconic 時(shí)間: 2025-3-23 15:21 作者: incontinence 時(shí)間: 2025-3-23 19:32
https://doi.org/10.1007/978-3-531-18997-0 and has recently gained momentum as a commercial manufacturing platform for human and veterinary vaccines. The three key components of ICT are the Lepidopteran insect cell line, the baculovirus vector, and the growth medium. Insect cell growth media have evolved significantly in the past five decad作者: 公豬 時(shí)間: 2025-3-23 23:23 作者: FID 時(shí)間: 2025-3-24 06:17 作者: 協(xié)奏曲 時(shí)間: 2025-3-24 08:25
Quantitative Befragung mit Kindernchapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore作者: 利用 時(shí)間: 2025-3-24 12:49 作者: 大約冬季 時(shí)間: 2025-3-24 15:22 作者: Wallow 時(shí)間: 2025-3-24 21:06 作者: FRONT 時(shí)間: 2025-3-25 00:04 作者: 無(wú)動(dòng)于衷 時(shí)間: 2025-3-25 06:41 作者: 生意行為 時(shí)間: 2025-3-25 08:39 作者: 漫不經(jīng)心 時(shí)間: 2025-3-25 14:00
https://doi.org/10.1007/978-1-4939-3043-2BEVS; RNAi; baculovirus molecular biology; biopesticides; express genes; generating envelop-modified bacu作者: MORT 時(shí)間: 2025-3-25 17:35
978-1-4939-7978-3Springer Science+Business Media, LLC 2016作者: Bronchial-Tubes 時(shí)間: 2025-3-25 20:50 作者: BUCK 時(shí)間: 2025-3-26 04:06 作者: 我正派 時(shí)間: 2025-3-26 08:05
Management pers?nlicher beruflicher Zieled using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.作者: GOAT 時(shí)間: 2025-3-26 11:15 作者: 吼叫 時(shí)間: 2025-3-26 13:31 作者: 豐滿(mǎn)有漂亮 時(shí)間: 2025-3-26 18:56 作者: Carbon-Monoxide 時(shí)間: 2025-3-26 23:20
Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus Systemd using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.作者: Antarctic 時(shí)間: 2025-3-27 04:39
Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression reparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed.作者: antecedence 時(shí)間: 2025-3-27 05:49 作者: Dealing 時(shí)間: 2025-3-27 10:38
Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculovirusesre) and long-term (frozen in liquid nitrogen) storage of cell lines, as well as quality control procedures for the cultures. Methods for storing baculoviruses for use in cell cultures and issues of concern when using cell cultures for their production and study are also described.作者: AND 時(shí)間: 2025-3-27 14:19
Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System protocols for titer estimation in 96-well plates, expression optimization in 24-well plates, and recombinant protein expression from adherent and suspension cultures in six-well plates and in 50 mL insect cell cultures.作者: 影響深遠(yuǎn) 時(shí)間: 2025-3-27 18:07
Book 2016Latest editionprocedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with 作者: 玩笑 時(shí)間: 2025-3-28 00:34 作者: conflate 時(shí)間: 2025-3-28 02:59 作者: Ganglion 時(shí)間: 2025-3-28 10:16
1064-3745 atory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Baculovirus and Insect Cell Expression Protocols, Third Edition. aims to not only aid the user in succ978-1-4939-7978-3978-1-4939-3043-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Decongestant 時(shí)間: 2025-3-28 14:06
Book 2016Latest editions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Baculovirus and Insect Cell Expression Protocols, Third Edition. aims to not only aid the user in succ作者: 踉蹌 時(shí)間: 2025-3-28 15:23 作者: 發(fā)炎 時(shí)間: 2025-3-28 19:52 作者: 金絲雀 時(shí)間: 2025-3-28 23:34 作者: antedate 時(shí)間: 2025-3-29 04:28 作者: Agnosia 時(shí)間: 2025-3-29 09:43
Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors by the bioreactor unit. The Cellbag can be filled with medium and cells and maintained at a set temperature. The rocking motion, which is adjusted through angle and rock speed settings, provides mixing of oxygen (and CO., which is used to control pH in mammalian cell cultures) from the headspace cr作者: ventilate 時(shí)間: 2025-3-29 12:09 作者: 不如屎殼郎 時(shí)間: 2025-3-29 15:59 作者: 與野獸博斗者 時(shí)間: 2025-3-29 20:23 作者: Mangle 時(shí)間: 2025-3-30 03:54
Laura Marie Wingender,Hans-Georg Wolff the isolation of recombinant baculoviruses (Chapter . provides a full account of the different systems and transfer vectors available). The practical details cover: transfection of insect cells with either virus DNA or virus DNA and plasmid transfer vector; a reliable plaque-assay method that can b作者: 易改變 時(shí)間: 2025-3-30 04:19 作者: Fsh238 時(shí)間: 2025-3-30 11:11
https://doi.org/10.1007/978-3-531-90142-8 by the bioreactor unit. The Cellbag can be filled with medium and cells and maintained at a set temperature. The rocking motion, which is adjusted through angle and rock speed settings, provides mixing of oxygen (and CO., which is used to control pH in mammalian cell cultures) from the headspace cr作者: FAZE 時(shí)間: 2025-3-30 15:44 作者: photopsia 時(shí)間: 2025-3-30 19:47 作者: WAX 時(shí)間: 2025-3-31 00:13 作者: amenity 時(shí)間: 2025-3-31 01:46 作者: sterilization 時(shí)間: 2025-3-31 06:54
Recombinant Baculovirus Isolationge in which insect cells are transfected with either the virus genome alone (Bac-to-Bac. or BaculoDirect?, Invitrogen) or virus genome and transfer vector. In the latter case, this allows the natural process of homologous recombination to transfer the foreign gene, under control of the polyhedrin or作者: MAG 時(shí)間: 2025-3-31 09:11 作者: 樹(shù)膠 時(shí)間: 2025-3-31 15:29 作者: 玉米 時(shí)間: 2025-3-31 18:36
Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculovirusesare presented that are appropriate for different cell lines dependent upon the growth characteristics (in particular, cell attachment properties) of the cells of interest. In addition to the routine maintenance of cells in active culture, methods are also described for both short-term (low temperatu作者: 傲慢物 時(shí)間: 2025-3-31 21:51
