標(biāo)題: Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2007Latest edition Humana Press 2007 Expression.Protein.cell lin [打印本頁] 作者: HAVEN 時(shí)間: 2025-3-21 17:10
書目名稱Baculovirus and Insect Cell Expression Protocols影響因子(影響力)
書目名稱Baculovirus and Insect Cell Expression Protocols影響因子(影響力)學(xué)科排名
書目名稱Baculovirus and Insect Cell Expression Protocols網(wǎng)絡(luò)公開度
書目名稱Baculovirus and Insect Cell Expression Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Baculovirus and Insect Cell Expression Protocols被引頻次
書目名稱Baculovirus and Insect Cell Expression Protocols被引頻次學(xué)科排名
書目名稱Baculovirus and Insect Cell Expression Protocols年度引用
書目名稱Baculovirus and Insect Cell Expression Protocols年度引用學(xué)科排名
書目名稱Baculovirus and Insect Cell Expression Protocols讀者反饋
書目名稱Baculovirus and Insect Cell Expression Protocols讀者反饋學(xué)科排名
作者: grandiose 時(shí)間: 2025-3-21 22:00
Matthias Vogelgesang,Andrea Schauertion of a recombinant gene into the virus genome without the requirement for a transfer vector. The information provided should enable new users of the system to choose those reagents most suitable for their purposes.作者: Reclaim 時(shí)間: 2025-3-22 00:28 作者: 易改變 時(shí)間: 2025-3-22 06:30 作者: 手術(shù)刀 時(shí)間: 2025-3-22 09:17
Leasing, Factoring und Forfaitierung,on transfection of larvae with recombinant viral DNA and does not require cultured insect cells as an intermediate stage. We suggest that small-to mid-scale recombinant protein production (mg-g level) can be achieved in . larvae with relative ease.作者: BIAS 時(shí)間: 2025-3-22 16:10
Introduction to Baculovirus Molecular Biologyst cell has innate defenses against baculoviruses in the form of an apoptotic response to virus invasion. Baculoviruses counter this by encoding apoptotic-suppressors, which also appear to have a role in determining the host range of the virus. Also of importance to our understanding of baculovirus 作者: HEDGE 時(shí)間: 2025-3-22 19:17
Baculovirus Transfer Vectorstion of a recombinant gene into the virus genome without the requirement for a transfer vector. The information provided should enable new users of the system to choose those reagents most suitable for their purposes.作者: 抱負(fù) 時(shí)間: 2025-3-22 22:14
Recombinant Baculovirus Isolationthe different systems and transfer vectors available). The practical details cover: transfection of insect cells with either virus DNA or virus DNA and plasmid transfer vector; a reliable plaque-assay method that can be used to separate recombinant virus from parental (nonrecombinant) virus where th作者: RAFF 時(shí)間: 2025-3-23 04:00
Development of Serum-Free Media for Lepidopteran Insect Cell Lineszed substances (e.g., surfactants and shear damage protecting chemicals). These supplements need to complement the defined, synthetic basal medium to ensure that the fundamental nutritional needs of the cells are satisfied. Although there is a significant number of proprietary serum-free and low-pro作者: guardianship 時(shí)間: 2025-3-23 09:28 作者: 斗爭(zhēng) 時(shí)間: 2025-3-23 10:24 作者: 把…比做 時(shí)間: 2025-3-23 14:45
Matthias Vogelgesang,Andrea Schauering advanced online monitoring of physiological parameters such as biovolume and respiration activity for batch and fed-batch insect cell cultures along with their role in operating high cell density cultures.作者: ABASH 時(shí)間: 2025-3-23 18:24 作者: Trigger-Point 時(shí)間: 2025-3-24 00:32 作者: 險(xiǎn)代理人 時(shí)間: 2025-3-24 04:34 作者: ERUPT 時(shí)間: 2025-3-24 07:26 作者: Outspoken 時(shí)間: 2025-3-24 12:49
Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insec insect cells allows mass production of VLPs in a proven scaleable system. This chapter focuses on the methodology, based on the work done in our lab, for the production of AAV-like particles and vectors in a BEVS/insect cell system.作者: 雇傭兵 時(shí)間: 2025-3-24 15:57 作者: 制定法律 時(shí)間: 2025-3-24 21:13
Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus Systemf the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.作者: 適宜 時(shí)間: 2025-3-25 00:40 作者: Bombast 時(shí)間: 2025-3-25 04:44 作者: oxidize 時(shí)間: 2025-3-25 11:22 作者: groggy 時(shí)間: 2025-3-25 14:44
Handbuch Innovative Wirtschaftsf?rderungf the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.作者: Affectation 時(shí)間: 2025-3-25 19:51
Handbuch Innovative Wirtschaftsf?rderung and long-term (frozen in liquid nitrogen) storage of cell lines, as well as quality control procedures for the cultures. Methods for storing baculoviruses for use in cell cultures and issues of concern when using cell cultures for their production and study are also described.作者: 變化無常 時(shí)間: 2025-3-25 23:04 作者: Inexorable 時(shí)間: 2025-3-26 01:36
1064-3745 l lines and provides methods for isolating new cell lines.Nu.Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides a作者: inchoate 時(shí)間: 2025-3-26 04:26 作者: 步履蹣跚 時(shí)間: 2025-3-26 11:44
Introduction to Baculovirus Molecular Biologyation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in insect larvae. Baculovirus gene expression occurs in an ordered cascade, regulated by early, la作者: Protein 時(shí)間: 2025-3-26 13:44
Baculovirus Transfer Vectorshen cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available. It is impractical to list every transfer vector that has ever been used. Instead, we focus on those that are available commer作者: 珊瑚 時(shí)間: 2025-3-26 19:48
Recombinant Baculovirus Isolationge in which insect cells are transfected with either the virus genome alone (Bac-to-Bac? or BaculoDirect?, Invitrogen) or virus genome and transfer vector. In the latter case, this allows the natural process of homologous recombination to transfer the foreign gene, under control of the polyhedrin or作者: 無力更進(jìn) 時(shí)間: 2025-3-27 00:57 作者: Conquest 時(shí)間: 2025-3-27 03:44
Development of Serum-Free Media for Lepidopteran Insect Cell Linesion systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring high yield of baculovirus as well as the strong expression of recombinant proteins. Vertebrate 作者: 提名的名單 時(shí)間: 2025-3-27 08:31 作者: Assemble 時(shí)間: 2025-3-27 13:08
Small-Scale Protein Production With the Baculovirus Expression Vector Systemtem (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and express作者: 舊式步槍 時(shí)間: 2025-3-27 14:39
Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression . Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, metho作者: debacle 時(shí)間: 2025-3-27 19:16 作者: 壕溝 時(shí)間: 2025-3-28 00:45 作者: gruelling 時(shí)間: 2025-3-28 04:36
Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insecnsect cell system. The production and scale-up of these particles, which are mostly sought as candidate vaccines, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. Recently, adeno-associated viral (AAV) vectors, which can be pot作者: affluent 時(shí)間: 2025-3-28 09:52
Baculovirus and Insect Cell Expression Protocols978-1-59745-457-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: interlude 時(shí)間: 2025-3-28 13:51 作者: 污穢 時(shí)間: 2025-3-28 18:19
https://doi.org/10.1007/978-3-658-22797-5 model system (e.g., for studying apoptosis). The fundamental techniques involved in these applications are described throughout this book. In this chapter, the most widely techniques are summarized and the reader is directed to detailed information found elsewhere in this book. Furthermore, many us作者: 演繹 時(shí)間: 2025-3-28 18:47
Jennifer Olearczyk,Simone Kauffeldation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in insect larvae. Baculovirus gene expression occurs in an ordered cascade, regulated by early, la作者: 悶熱 時(shí)間: 2025-3-29 00:17
Matthias Vogelgesang,Andrea Schauerhen cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available. It is impractical to list every transfer vector that has ever been used. Instead, we focus on those that are available commer作者: Canvas 時(shí)間: 2025-3-29 05:50
Matthias Vogelgesang,Philip Pongratzge in which insect cells are transfected with either the virus genome alone (Bac-to-Bac? or BaculoDirect?, Invitrogen) or virus genome and transfer vector. In the latter case, this allows the natural process of homologous recombination to transfer the foreign gene, under control of the polyhedrin or作者: analogous 時(shí)間: 2025-3-29 10:01 作者: inhumane 時(shí)間: 2025-3-29 12:16
Matthias Vogelgesang,Philip Pongratzion systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring high yield of baculovirus as well as the strong expression of recombinant proteins. Vertebrate 作者: 慢慢流出 時(shí)間: 2025-3-29 16:48 作者: 砍伐 時(shí)間: 2025-3-29 21:11
Entwicklung der Wirtschaftsf?rderungtem (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and express作者: 噴油井 時(shí)間: 2025-3-30 02:45 作者: circumvent 時(shí)間: 2025-3-30 04:55
Leasing, Factoring und Forfaitierung,ailable to support expression needs from small to large scale. They offer many advantages over stirred tank and airlift reactors, including simple operation, ease of setup and clean-up, and minimal utility requirements. The design consists of a platform rocker onto which presterilized and assembled 作者: 集合 時(shí)間: 2025-3-30 08:33
