作者: indigenous 時(shí)間: 2025-3-21 20:55 作者: FEAS 時(shí)間: 2025-3-22 03:40
Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencingy where each genomic fragment has an equal chance of being represented is critical to the success of the WGSA. For many phages, there are regions of the genome likely to be under-represented in the shotgun library, which results in more gaps in the shotgun assembly than predicted by the Poisson dist作者: rectum 時(shí)間: 2025-3-22 04:37 作者: 施舍 時(shí)間: 2025-3-22 09:12 作者: calorie 時(shí)間: 2025-3-22 16:39 作者: 終端 時(shí)間: 2025-3-22 19:36 作者: antiandrogen 時(shí)間: 2025-3-22 22:03
Molecular Phylogenetics: Testing Evolutionary Hypothesesevolutionary tree. This methodology is known as molecular phylogenetics and may be the most informative means for exploring phage evolution, since there are few morphological features that can be used to differentiate between these tiny biological entities. In addition, phage genomes can be mosaic, 作者: diskitis 時(shí)間: 2025-3-23 01:26 作者: 炸壞 時(shí)間: 2025-3-23 05:39 作者: 吵鬧 時(shí)間: 2025-3-23 10:35 作者: Scintigraphy 時(shí)間: 2025-3-23 14:21 作者: 就職 時(shí)間: 2025-3-23 18:51 作者: Feckless 時(shí)間: 2025-3-23 23:24
Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinttify the diversity of natural viral communities. Currently, two different methodological approaches are widely used to obtain genetic fingerprints of natural phage communities. While the separation of different viral genomes with pulsed field gel electrophoresis (PFGE) is based on the size of the ge作者: Small-Intestine 時(shí)間: 2025-3-24 04:13
Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenomethin the universally conserved gene for small subunit ribosomal RNA (16S rDNA). A plethora of methodologies for characterizing the diversity and composition of bacterial communities is based on sequence polymorphisms within this single gene. By comparison, no gene is universally shared among viruses作者: 專橫 時(shí)間: 2025-3-24 08:27
Armin Lechler,Jan Schlechtendahletween phages which infect fairly divergent taxa. As more sequence data become available through both metagenomic approaches and the sequencing of complete bacteriophage genomes, PCR primers can be further refined and thus it should be an increasingly useful tool for bacteriophage biology.作者: 呼吸 時(shí)間: 2025-3-24 14:12
Stefan Feldmann,Birgit Vogel-Heuserion and quantification of high quality RNA from infected bacterial cells, using the marine cyanobacterium WH7803 and the phage S-PM2 as a case study. This protocol can be modified to extract RNA from the host/bacteriophage of interest.作者: 僵硬 時(shí)間: 2025-3-24 15:13
Kay Fürstenberg,Christopher Kirscho how probe length, secondary structure, free energy, probe orientation and amplification all have to be taken into account. As means of an example, the conditions used for the hybridisation of an array designed to be specific to the cyanophage S-PM2 is detailed.作者: stress-test 時(shí)間: 2025-3-24 19:00 作者: 潰爛 時(shí)間: 2025-3-24 23:36 作者: 殺死 時(shí)間: 2025-3-25 06:04 作者: dura-mater 時(shí)間: 2025-3-25 08:23
Dieter Steegmüller,Michael ZürnBasic Local Alignment Search Tool (BLASTX) to identify genes through homologs, a variety of tools are discussed by their creators. These include for genome annotation: GeneMark, Artemis, and BASys; and, for genome comparisons: Artemis Comparison Tool (ACT), Mauve, CoreGenes, and GeneOrder.作者: Palatial 時(shí)間: 2025-3-25 12:47 作者: 誹謗 時(shí)間: 2025-3-25 15:52
Plug&Play-F?rdertechnik in der Industrie 4.0ocuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.作者: 瘙癢 時(shí)間: 2025-3-25 22:08 作者: 小說(shuō) 時(shí)間: 2025-3-26 00:21
Characterization of DNA Motifs with Particular Reference to Promoters and Terminatorsand resources to identify regulatory elements are listed and discussed. Combining the available web-resources and literature data optimizes these predictions and can thus aid in a more directed experimental identification of these regulatory elements.作者: 災(zāi)難 時(shí)間: 2025-3-26 07:50 作者: 褲子 時(shí)間: 2025-3-26 10:57
PCR and Partial Sequencing of Bacteriophage Genomesetween phages which infect fairly divergent taxa. As more sequence data become available through both metagenomic approaches and the sequencing of complete bacteriophage genomes, PCR primers can be further refined and thus it should be an increasingly useful tool for bacteriophage biology.作者: mighty 時(shí)間: 2025-3-26 14:51 作者: 善于 時(shí)間: 2025-3-26 20:39
Michael ten Hompel,Michael Henkeacteriophage DNA from one or two 9?cm L-agar plates. For these preps, the use of agarose in the top agar is recommended to avoid any restriction inhibitors that may be present in some agar preparations.作者: 忍受 時(shí)間: 2025-3-26 22:46 作者: 優(yōu)雅 時(shí)間: 2025-3-27 01:40
Kleinskalige, cyber-physische F?rdertechnikal viral communities. Finger printing techniques are a relatively fast and cheap tool to assess the diversity of environmental viruses. Together, PFGE and DGGE provide useful tools to study viral ecology in natural habitats.作者: CHIDE 時(shí)間: 2025-3-27 09:01 作者: Basilar-Artery 時(shí)間: 2025-3-27 10:35
Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriopcomplete genome sequence determination does not by itself elucidate the nature of these ends, so directed experimental analysis is usually required to understand the nature of the virion chromosome ends. This chapter discusses these methods.作者: 社團(tuán) 時(shí)間: 2025-3-27 16:26 作者: 財(cái)產(chǎn) 時(shí)間: 2025-3-27 18:34
Dieter Steegmüller,Michael ZürnStandard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40–50?kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200?bp to 12?Mb.作者: Cardiac-Output 時(shí)間: 2025-3-28 00:18
Determination of Bacteriophage Genome Size by Pulsed-Field Gel ElectrophoresisStandard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40–50?kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200?bp to 12?Mb.作者: Gyrate 時(shí)間: 2025-3-28 03:26
Michael ten Hompel,Michael Henkevery or subject to significant genomic contamination. Recently though, new methods have come along that allow the purification of DNA from plate lysates that are not only capable of high yield but also, for all intents and purposes, free of genomic contamination (i.e. no visible genomic contaminatio作者: 條街道往前推 時(shí)間: 2025-3-28 07:54
Armin Lechler,Jan Schlechtendahlhniques are described including hydrolysis of the DNA to the level of bases or nucleosides and separation by paper chromatography or HPLC. Spectroscopic and spectrofluorometric procedures are also outlined.作者: Lament 時(shí)間: 2025-3-28 13:13
Wilhelm Bauer,Bernd Dworschak,Helmut Zaisery where each genomic fragment has an equal chance of being represented is critical to the success of the WGSA. For many phages, there are regions of the genome likely to be under-represented in the shotgun library, which results in more gaps in the shotgun assembly than predicted by the Poisson dist作者: 富足女人 時(shí)間: 2025-3-28 15:17
Armin Lechler,Jan Schlechtendahlacterial cells or purified viruses. The limitations are that you have to know what you are looking for in order to find it. Although the bacteriophage world does not have the advantage of a conserved gene, present in all members, there are many phage genes that do show nucleotide conservation even b作者: 極為憤怒 時(shí)間: 2025-3-28 19:15
Dieter Steegmüller,Michael Zürn which encode tRNAs and those which produce proteins. After a general introduction on the properties of protein-encoding genes and the utility of the Basic Local Alignment Search Tool (BLASTX) to identify genes through homologs, a variety of tools are discussed by their creators. These include for g作者: Arrhythmia 時(shí)間: 2025-3-29 00:59
Dieter Steegmüller,Michael Zürnypes. These linear chromosomes can have one of several known types of termini as follows: cohesive ends (.- or .-single-strand extensions), circularly permuted direct terminal repeats, short or long exact direct terminal repeats, terminal host DNA sequences, or covalently bound terminal proteins. Th作者: 絆住 時(shí)間: 2025-3-29 06:49 作者: 疲憊的老馬 時(shí)間: 2025-3-29 08:48
Daniel Regulin,Birgit Vogel-Heuserevolutionary tree. This methodology is known as molecular phylogenetics and may be the most informative means for exploring phage evolution, since there are few morphological features that can be used to differentiate between these tiny biological entities. In addition, phage genomes can be mosaic, 作者: Amplify 時(shí)間: 2025-3-29 14:39 作者: frivolous 時(shí)間: 2025-3-29 18:14 作者: 陳腐思想 時(shí)間: 2025-3-29 20:44
Kay Fürstenberg,Christopher Kirschreports to date of microarrays being used to investigate phage biology. This chapter aims to provide an overview of how to design and implement a microarray experiment to investigate phage biology..Given the nature of microarrays being specific to an organism, each will provide a number of unique is作者: anniversary 時(shí)間: 2025-3-30 02:29 作者: Innovative 時(shí)間: 2025-3-30 05:32
Plug&Play-F?rdertechnik in der Industrie 4.0tein sequences within databases..This chapter provides a short overview of MS techniques used in the identification of phage structural proteins and focuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. 作者: maroon 時(shí)間: 2025-3-30 11:08 作者: 半圓鑿 時(shí)間: 2025-3-30 14:59 作者: 秘密會(huì)議 時(shí)間: 2025-3-30 18:33 作者: 語(yǔ)言學(xué) 時(shí)間: 2025-3-30 22:47
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180354.jpg作者: Euphonious 時(shí)間: 2025-3-31 02:22 作者: Organonitrile 時(shí)間: 2025-3-31 05:12
Armin Lechler,Jan Schlechtendahlhniques are described including hydrolysis of the DNA to the level of bases or nucleosides and separation by paper chromatography or HPLC. Spectroscopic and spectrofluorometric procedures are also outlined.作者: Defense 時(shí)間: 2025-3-31 10:29
https://doi.org/10.1007/978-1-60327-565-1DNA; Laboratory; Microarray; PCR; Polyacrylamid-Gelelektrophorese; Promoter; Termination; bacteria; molecula作者: 維持 時(shí)間: 2025-3-31 16:37
