標(biāo)題: Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski Book 2009 Humana Press 2009 Bacteria.Microbial ecology.Microbia [打印本頁] 作者: 譴責(zé) 時間: 2025-3-21 19:45
書目名稱Bacteriophages影響因子(影響力)
書目名稱Bacteriophages影響因子(影響力)學(xué)科排名
書目名稱Bacteriophages網(wǎng)絡(luò)公開度
書目名稱Bacteriophages網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bacteriophages被引頻次
書目名稱Bacteriophages被引頻次學(xué)科排名
書目名稱Bacteriophages年度引用
書目名稱Bacteriophages年度引用學(xué)科排名
書目名稱Bacteriophages讀者反饋
書目名稱Bacteriophages讀者反饋學(xué)科排名
作者: dissolution 時間: 2025-3-21 23:05 作者: infantile 時間: 2025-3-22 02:03
Handbuch Human Resource Managementplaques is a central technique to phage studies, yet appears to be under appreciated by phage workers in terms of its complexity. Third, selective pressures acting on phage during plaque growth differ from those seen during broth growth..In this chapter we will discuss just what a plaque is, how one作者: 女歌星 時間: 2025-3-22 06:26
Methods for the Isolation of Viruses from Environmental Samplesbtaining a concentrate of viruses from an environmental sample. Culture-dependent methods also rely on viruses being present at a high enough abundance to detect. Here, methodological details for the isolation and concentration of viruses from water, soil, and aquatic sediment samples are covered in作者: chronicle 時間: 2025-3-22 11:44 作者: 厭煩 時間: 2025-3-22 15:20
Bacteriophage Plaques: Theory and Analysisplaques is a central technique to phage studies, yet appears to be under appreciated by phage workers in terms of its complexity. Third, selective pressures acting on phage during plaque growth differ from those seen during broth growth..In this chapter we will discuss just what a plaque is, how one作者: ovation 時間: 2025-3-22 18:52
Book 2009cols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls...Thorough and cutting-edge, .Bacteriophages: Methods and Protocols. is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject作者: 弓箭 時間: 2025-3-22 23:50
1064-3745 s...Thorough and cutting-edge, .Bacteriophages: Methods and Protocols. is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject978-1-61737-715-0978-1-60327-164-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 欲望小妹 時間: 2025-3-23 02:34 作者: ITCH 時間: 2025-3-23 08:20 作者: 提升 時間: 2025-3-23 12:54 作者: 左右連貫 時間: 2025-3-23 16:35
1064-3745 y-to-use style.Designed for modular use, providing a potenti.Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have?inspired increased interest within the scientific community. .Bacteriophages:作者: Nerve-Block 時間: 2025-3-23 20:33
https://doi.org/10.1007/978-3-658-25314-1ws the detection and discrimination of a wide variety of viruses of different morphology, genome type, and size. The present paper describes an optimized protocol for the enumeration of bacteriophages using a standard benchtop flow cytometer.作者: Negligible 時間: 2025-3-24 00:52
Hochtemperatur-Korrosionsschutzschichten,ues and results. In describing viruses, the choice of methods depends on structure and taxonomical position of viruses. The study of isometric, filamentous, and pleomorphic viruses requires more detailed investigations than that of tailed species.作者: Pepsin 時間: 2025-3-24 05:14
Handbuch Hochtemperatur-Werkstofftechnikndonucleases, the relative efficiency of plating (EOP), that is, the titer of the phage on a given bacterial cell line compared to the maximum titer observed, may vary considerably. This chapter describes rapid procedures for determining the host range and relative EOP on each host of any phage.作者: instill 時間: 2025-3-24 07:21
Enumeration of Bacteriophages Using Flow Cytometryws the detection and discrimination of a wide variety of viruses of different morphology, genome type, and size. The present paper describes an optimized protocol for the enumeration of bacteriophages using a standard benchtop flow cytometer.作者: 燒瓶 時間: 2025-3-24 12:28 作者: Cytokines 時間: 2025-3-24 16:52
Phage Host Range and Efficiency of Platingndonucleases, the relative efficiency of plating (EOP), that is, the titer of the phage on a given bacterial cell line compared to the maximum titer observed, may vary considerably. This chapter describes rapid procedures for determining the host range and relative EOP on each host of any phage.作者: Accrue 時間: 2025-3-24 20:02 作者: hypertension 時間: 2025-3-25 00:27 作者: Mundane 時間: 2025-3-25 03:32
Isolation of Viruses from High Temperature Environmentses are problematic because of our rudimentary knowledge of viral diversity in these environments and the lack of universally conserved signatures that can be used for virus detection and isolation. Here we discuss both culture-based and culture-independent techniques for detection and isolation of viruses from acidic thermal features.作者: incision 時間: 2025-3-25 09:21
Book 2009olution have?inspired increased interest within the scientific community. .Bacteriophages: Methods and Protocols .pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its wel作者: 高度表 時間: 2025-3-25 15:29 作者: extemporaneous 時間: 2025-3-25 16:22
Isolation of Cyanophages from Aquatic Environmentstwo dominant genera .s and . contribute significantly to primary production in the oceans. Cyanophages that infect marine cyanobacteria were first isolated in the early 1990s and it is now known that by lysing their host cells they play an important role in the microbial loop and alter biogeochemica作者: gorgeous 時間: 2025-3-25 23:31 作者: Insensate 時間: 2025-3-26 03:00 作者: Breach 時間: 2025-3-26 08:22 作者: 兵團(tuán) 時間: 2025-3-26 11:56 作者: indoctrinate 時間: 2025-3-26 13:06 作者: Assemble 時間: 2025-3-26 19:28
Phage Classification and Characterizationkaryote viruses have been examined in the electron microscope. Classification has a predictive value and is invaluable to control experimental techniques and results. In describing viruses, the choice of methods depends on structure and taxonomical position of viruses. The study of isometric, filame作者: jaunty 時間: 2025-3-26 22:09
Phage Host Range and Efficiency of Platingistics of a particular bacterial virus. Because of host factors such as masking by O antigens that affects injection and the presence of restriction endonucleases, the relative efficiency of plating (EOP), that is, the titer of the phage on a given bacterial cell line compared to the maximum titer o作者: 營養(yǎng) 時間: 2025-3-27 03:32
Bacteriophage Plaques: Theory and Analysismay be distinguished in terms of their spatial structure, i.e., the degree to which they limit diffusion, motility, and environmental mixing. Well-mixed broth, for example, represents the microbiological ideal of a non-spatially structured environment. Agar, by contrast, imposes significant limitati作者: explicit 時間: 2025-3-27 08:27
9 Autor und Erz?hler – Frühe Neuzeitic and porous media environments (soils and sediments) viral abundance exceeds that of co-occurring host populations by 10–100-fold. If current estimates based on metagenome DNA sequence data are correct, then viruses represent the largest reservoir of unknown genetic diversity on Earth. Microscopy 作者: Hemiparesis 時間: 2025-3-27 12:32 作者: AXIOM 時間: 2025-3-27 17:25
,Reinigung der Mauerwerksoberfl?chen,g. These extreme environments tend to have a low biomass (typically <10. cells/ml) and low free viral abundance (10.-10.particles/ml) compared to eutrophic freshwater lakes (.) or near- shore marine environments (.). Establishing laboratory cultures from these environments pose challenges due to the作者: 驚呼 時間: 2025-3-27 20:00
https://doi.org/10.1007/978-3-642-25468-0ng microscopy and DNA sequence derived from community sequencing. The assumption is that these procedures will succeed for most, if not all, viruses. However, we have found that some bacteriophages are in a category of viruses that are not detected by any of these classical procedures. Given that th作者: 聲音刺耳 時間: 2025-3-28 00:43
Handbuch Hochtemperatur-Werkstofftechniky. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.作者: 大氣層 時間: 2025-3-28 02:20
Handbuch Hochtemperatur-Werkstofftechnikticles by collection on a filter. The nucleic acid in the virus particles is then stained with a fluorescent stain and the sample viewed with an epifluorescence microscope. The method was originally developed to determine the abundance of virus particles in water samples, however the protocol has be作者: 大看臺 時間: 2025-3-28 07:45
https://doi.org/10.1007/978-3-658-25314-1y. The development of sensitive nucleic acid stains, in combination with flow cytometric techniques, has changed this. The flow cytometric method allows the detection and discrimination of a wide variety of viruses of different morphology, genome type, and size. The present paper describes an optimi作者: cipher 時間: 2025-3-28 13:33
Hochtemperatur-Korrosionsschutzschichten,karyote viruses have been examined in the electron microscope. Classification has a predictive value and is invaluable to control experimental techniques and results. In describing viruses, the choice of methods depends on structure and taxonomical position of viruses. The study of isometric, filame作者: Rustproof 時間: 2025-3-28 17:45 作者: Painstaking 時間: 2025-3-28 19:06 作者: right-atrium 時間: 2025-3-28 23:25
Enumeration of Bacteriophages Using the Small Drop Plaque Assay Systemy. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.作者: 書法 時間: 2025-3-29 06:16 作者: 恃強(qiáng)凌弱 時間: 2025-3-29 08:10 作者: Dealing 時間: 2025-3-29 12:49 作者: Silent-Ischemia 時間: 2025-3-29 15:59
Handbuch Historisches MauerwerkMost bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.作者: OGLE 時間: 2025-3-29 23:28
Hochtemperaturfestigkeit und -verformung,The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.作者: 縮短 時間: 2025-3-30 01:30
https://doi.org/10.1007/978-3-8348-9907-1A method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.作者: 煩憂 時間: 2025-3-30 07:04
Zyklische Festigkeit und Verformung,Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.作者: Offensive 時間: 2025-3-30 08:57 作者: 發(fā)誓放棄 時間: 2025-3-30 14:57 作者: Myosin 時間: 2025-3-30 17:32 作者: 功多汁水 時間: 2025-3-30 20:58 作者: AMEND 時間: 2025-3-31 03:02
Enumeration of Bacteriophages by Double Agar Overlay Plaque AssayThe determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.作者: 羞辱 時間: 2025-3-31 05:40
Enumeration of Bacteriophages by the Direct Plating Plaque AssayA method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.作者: 打火石 時間: 2025-3-31 12:24
Basic Phage Electron MicroscopyNegative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.作者: Endearing 時間: 2025-3-31 14:07 作者: 令人發(fā)膩 時間: 2025-3-31 19:28
